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Carolyn Bertozzi Lab Plasmids


The Carolyn Bertozzi Lab has deposited plasmids at Addgene for distribution to the research community. Addgene is a non-profit plasmid repository dedicated to improving life science research.

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Title Authors Publication
A riboswitch-based inducible gene expression system for mycobacteria. Seeliger JC, Topp S, Sogi KM, Previti ML, Gallivan JP, Bertozzi CR PLoS One. 2012;7(1):e29266. Epub 2012 Jan 18.
Site-specific chemical modification of recombinant proteins produced in mammalian cells by using the genetically encoded aldehyde tag. Wu P, Shui W, Carlson BL, Hu N, Rabuka D, Lee J, Bertozzi CR Proc Natl Acad Sci U S A. 2009 Mar 3. 106(9):3000-5.
Conditional glycosylation in eukaryotic cells using a biocompatible chemical inducer of dimerization. Czlapinski JL, Schelle MW, Miller LW, Laughlin ST, Kohler JJ, Cornish VW, Bertozzi CR J Am Chem Soc. 2008 Oct 8. 130(40):13186-7.
Introducing genetically encoded aldehydes into proteins. Carrico IS, Carlson BL, Bertozzi CR Nat Chem Biol. 2007 Jun . 3(6):321-2.
Plasmid Gene/Insert Vector Type Publication Hidden Extra Search Info  
pBAD/myc-his A Rv0712 (FGE) Formylglycine-generating enzyme Bacterial Expression Introducing genetically encoded aldehydes into proteins. Carrico et al Nat Chem Biol. 2007 Jun . 3(6):321-2. Bacterial expression of Mycobacterium tuberculosis FGE pBAD/myc-his A Add to Cart
FKBP-myc FKBP (Homo sapiens) Mammalian Expression Conditional glycosylation in eukaryotic cells using a biocompatible chemical inducer of dimerization. Czlapinski et al J Am Chem Soc. 2008 Oct 8. 130(40):13186-7. pcDNA3.1/Zeo(+) FKBP1A RP11-314N13.2, FKBP-12, FKBP-1A, FKBP1, FKBP12, PKC12, PKCI2, PPIASE Add to Cart
2xFKBP-myc 2xFKBP (Homo sapiens) Mammalian Expression Conditional glycosylation in eukaryotic cells using a biocompatible chemical inducer of dimerization. Czlapinski et al J Am Chem Soc. 2008 Oct 8. 130(40):13186-7. pcDNA3.1/Zeo(+) FKBP1A RP11-314N13.2, FKBP-12, FKBP-1A, FKBP1, FKBP12, PKC12, PKCI2, PPIASE Add to Cart
3xFKBP-myc 3xFKBP (Homo sapiens) Mammalian Expression Conditional glycosylation in eukaryotic cells using a biocompatible chemical inducer of dimerization. Czlapinski et al J Am Chem Soc. 2008 Oct 8. 130(40):13186-7. pcDNA3.1/Zeo(+) FKBP1A RP11-314N13.2, FKBP-12, FKBP-1A, FKBP1, FKBP12, PKC12, PKCI2, PPIASE Add to Cart
DHFR-myc DHFR Mammalian Expression Conditional glycosylation in eukaryotic cells using a biocompatible chemical inducer of dimerization. Czlapinski et al J Am Chem Soc. 2008 Oct 8. 130(40):13186-7. pcDNA3.1/Zeo(+) folA b0048, ECK0049, JW0047, tmrA Add to Cart
2xDHFR-myc 2xDHFR Mammalian Expression Conditional glycosylation in eukaryotic cells using a biocompatible chemical inducer of dimerization. Czlapinski et al J Am Chem Soc. 2008 Oct 8. 130(40):13186-7. pcDNA3.1/Zeo(+) folA b0048, ECK0049, JW0047, tmrA Add to Cart
3xDHFR-myc 3xDHFR Mammalian Expression Conditional glycosylation in eukaryotic cells using a biocompatible chemical inducer of dimerization. Czlapinski et al J Am Chem Soc. 2008 Oct 8. 130(40):13186-7. pcDNA3.1/Zeo(+) folA b0048, ECK0049, JW0047, tmrA Add to Cart
Vh-HA-DHFR DHFR Mammalian Expression Conditional glycosylation in eukaryotic cells using a biocompatible chemical inducer of dimerization. Czlapinski et al J Am Chem Soc. 2008 Oct 8. 130(40):13186-7. pcDNA3.1(+) folA b0048, ECK0049, JW0047, tmrA Add to Cart
Vh-HA-2xDHFR 2xDHFR Mammalian Expression Conditional glycosylation in eukaryotic cells using a biocompatible chemical inducer of dimerization. Czlapinski et al J Am Chem Soc. 2008 Oct 8. 130(40):13186-7. pcDNA3.1(+) folA b0048, ECK0049, JW0047, tmrA Add to Cart
Vh-HA-3xDHFR 3xDHFR Mammalian Expression Conditional glycosylation in eukaryotic cells using a biocompatible chemical inducer of dimerization. Czlapinski et al J Am Chem Soc. 2008 Oct 8. 130(40):13186-7. pcDNA3.1(+) folA b0048, ECK0049, JW0047, tmrA Add to Cart
Vh-HA-FKBP FKBP (Homo sapiens) Mammalian Expression Conditional glycosylation in eukaryotic cells using a biocompatible chemical inducer of dimerization. Czlapinski et al J Am Chem Soc. 2008 Oct 8. 130(40):13186-7. pcDNA3.1(+) FKBP1A RP11-314N13.2, FKBP-12, FKBP-1A, FKBP1, FKBP12, PKC12, PKCI2, PPIASE Add to Cart
Vh-HA-2xFKBP 2xFKBP (Homo sapiens) Mammalian Expression Conditional glycosylation in eukaryotic cells using a biocompatible chemical inducer of dimerization. Czlapinski et al J Am Chem Soc. 2008 Oct 8. 130(40):13186-7. pcDNA3.1(+) FKBP1A RP11-314N13.2, FKBP-12, FKBP-1A, FKBP1, FKBP12, PKC12, PKCI2, PPIASE Add to Cart
Vh-HA-3xFKBP 3xFKBP (Homo sapiens) Mammalian Expression Conditional glycosylation in eukaryotic cells using a biocompatible chemical inducer of dimerization. Czlapinski et al J Am Chem Soc. 2008 Oct 8. 130(40):13186-7. pcDNA3.1(+) FKBP1A RP11-314N13.2, FKBP-12, FKBP-1A, FKBP1, FKBP12, PKC12, PKCI2, PPIASE Add to Cart
Vh-HA-3xFRB 3xFRB (Homo sapiens) Mammalian Expression Conditional glycosylation in eukaryotic cells using a biocompatible chemical inducer of dimerization. Czlapinski et al J Am Chem Soc. 2008 Oct 8. 130(40):13186-7. pcDNA3.1(+) MTOR FRAP, FRAP1, FRAP2, RAFT1, RAPT1 Add to Cart
Vh-HA-2xFRB 2xFRB (Homo sapiens) Mammalian Expression Conditional glycosylation in eukaryotic cells using a biocompatible chemical inducer of dimerization. Czlapinski et al J Am Chem Soc. 2008 Oct 8. 130(40):13186-7. pcDNA3.1(+) MTOR FRAP, FRAP1, FRAP2, RAFT1, RAPT1 Add to Cart
Vh-HA-FRB FRB (Homo sapiens) Mammalian Expression Conditional glycosylation in eukaryotic cells using a biocompatible chemical inducer of dimerization. Czlapinski et al J Am Chem Soc. 2008 Oct 8. 130(40):13186-7. pcDNA3.1(+) MTOR FRAP, FRAP1, FRAP2, RAFT1, RAPT1 Add to Cart
3xFRB-myc 3xFRB (Homo sapiens) Mammalian Expression Conditional glycosylation in eukaryotic cells using a biocompatible chemical inducer of dimerization. Czlapinski et al J Am Chem Soc. 2008 Oct 8. 130(40):13186-7. pcDNA3.1/Zeo(+) MTOR FRAP, FRAP1, FRAP2, RAFT1, RAPT1 Add to Cart
2xFRB-myc 2xFRB (Homo sapiens) Mammalian Expression Conditional glycosylation in eukaryotic cells using a biocompatible chemical inducer of dimerization. Czlapinski et al J Am Chem Soc. 2008 Oct 8. 130(40):13186-7. pcDNA3.1/Zeo(+) MTOR FRAP, FRAP1, FRAP2, RAFT1, RAPT1 Add to Cart
FRB-myc FRB (Homo sapiens) Mammalian Expression Conditional glycosylation in eukaryotic cells using a biocompatible chemical inducer of dimerization. Czlapinski et al J Am Chem Soc. 2008 Oct 8. 130(40):13186-7. pcDNA3.1/Zeo(+) MTOR FRAP, FRAP1, FRAP2, RAFT1, RAPT1 Add to Cart
pcDNA3.1 Neo(+) hSUMF1 hSUMF1 (Homo sapiens) Mammalian Expression Site-specific chemical modification of recombinant proteins produced in mammalian cells by using the genetically encoded aldehyde tag. Wu et al Proc Natl Acad Sci U S A. 2009 Mar 3. 106(9):3000-5. pcDNA3.1 (+) SUMF1 UNQ3037, AAPA3037, FGE Add to Cart
pRibo BsaHind Bacterial Expression, Synthetic Biology ; Mycobacteria replicating vector A riboswitch-based inducible gene expression system for mycobacteria. Seeliger et al PLoS One. 2012;7(1):e29266. Epub 2012 Jan 18. pMV261 Add to Cart
pRibo EcoHind Bacterial Expression, Synthetic Biology ; Mycobacteria replicating vector A riboswitch-based inducible gene expression system for mycobacteria. Seeliger et al PLoS One. 2012;7(1):e29266. Epub 2012 Jan 18. pMV261 Add to Cart
pRiboS BsaHind Bacterial Expression, Synthetic Biology ; Mycobacteria replicating vector A riboswitch-based inducible gene expression system for mycobacteria. Seeliger et al PLoS One. 2012;7(1):e29266. Epub 2012 Jan 18. pMV261 Add to Cart
pRiboS EcoHind Bacterial Expression, Synthetic Biology A riboswitch-based inducible gene expression system for mycobacteria. Seeliger et al PLoS One. 2012;7(1):e29266. Epub 2012 Jan 18. pMV261 Add to Cart
pST5552 eGFP Bacterial Expression, Synthetic Biology ; Mycobacteria replicating vector A riboswitch-based inducible gene expression system for mycobacteria. Seeliger et al PLoS One. 2012;7(1):e29266. Epub 2012 Jan 18. Mycobacteria replicating vector expressing eGFP. pMV261 Add to Cart
pST5832 lacZ Bacterial Expression, Synthetic Biology ; Mycobacteria replicating vector A riboswitch-based inducible gene expression system for mycobacteria. Seeliger et al PLoS One. 2012;7(1):e29266. Epub 2012 Jan 18. pMV261 Add to Cart