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Danwei Huangfu Lab Plasmids


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Title Authors Publication
An iCRISPR Platform for Rapid, Multiplexable, and Inducible Genome Editing in Human Pluripotent Stem Cells. Gonzalez F, Zhu Z, Shi ZD, Lelli K, Verma N, Li QV, Huangfu D Cell Stem Cell. 2014 Jun 11. pii: S1934-5909(14)00205-7. doi: 10.1016/j.stem.2014.05.018.
A CRISPR/Cas-Mediated Selection-free Knockin Strategy in Human Embryonic Stem Cells. Zhu Z, Verma N, Gonzalez F, Shi ZD, Huangfu D Stem Cell Reports. 2015 May 28. pii: S2213-6711(15)00128-9. doi: 10.1016/j.stemcr.2015.04.016.
Genome Editing of Lineage Determinants in Human Pluripotent Stem Cells Reveals Mechanisms of Pancreatic Development and Diabetes. Zhu Z, Li QV, Lee K, Rosen BP, Gonzalez F, Soh CL, Huangfu D Cell Stem Cell. 2016 Apr 27. pii: S1934-5909(16)30002-9. doi: 10.1016/j.stem.2016.03.015.
Genome Editing in hPSCs Reveals GATA6 Haploinsufficiency and a Genetic Interaction with GATA4 in Human Pancreatic Development. Shi ZD, Lee K, Yang D, Amin S, Verma N, Li QV, Zhu Z, Soh CL, Kumar R, Evans T, Chen S, Huangfu D Cell Stem Cell. 2017 Feb 8. pii: S1934-5909(17)30001-2. doi: 10.1016/j.stem.2017.01.001.
ID Plasmid Gene/Insert Vector Type Publication Hidden Extra Search Info  
58409Puro-Cas9 donorhSpCas9 (Other)Mammalian Expression, CRISPRAn iCRISPR Platform for Rapid, Multiplexable, and Inducible Genome Editing in Human Pluripotent Stem Cells. Cell Stem Cell. 2014 Jun 11. pii: S1934-5909(14)00205-7. doi: 10.1016/j.stem.2014.05.018. Donor vector for genomic targeting of a Tetracycline-inducible Cas9 cassette to the human AAVS1/PPP1R12C locus N/A Add to Cart
58904piCRg EntryhSpCas9Mammalian ExpressionAn iCRISPR Platform for Rapid, Multiplexable, and Inducible Genome Editing in Human Pluripotent Stem Cells. Cell Stem Cell. 2014 Jun 11. pii: S1934-5909(14)00205-7. doi: 10.1016/j.stem.2014.05.018. Cas9/gRNA Entry expression plasmid Low copy Add to Cart
59025AAVS1-TALEN-LAAVS1-TALEN-L (Synthetic)Mammalian Expression, AAV, TALENAn iCRISPR Platform for Rapid, Multiplexable, and Inducible Genome Editing in Human Pluripotent Stem Cells. Cell Stem Cell. 2014 Jun 11. pii: S1934-5909(14)00205-7. doi: 10.1016/j.stem.2014.05.018. TALEN designed to target the human AAVS1/PPP1R12C locus pTALEN Add to Cart
59026AAVS1-TALEN-RAAVS1-TALEN-R (Synthetic)Mammalian Expression, AAV, TALENAn iCRISPR Platform for Rapid, Multiplexable, and Inducible Genome Editing in Human Pluripotent Stem Cells. Cell Stem Cell. 2014 Jun 11. pii: S1934-5909(14)00205-7. doi: 10.1016/j.stem.2014.05.018. TALEN designed to target the human AAVS1/PPP1R12C locus pTALEN Add to Cart
66964PDX1-2A-eGFPpancreatic and duodenal homeobox 1 (Homo sapiens)UnspecifiedA CRISPR/Cas-Mediated Selection-free Knockin Strategy in Human Embryonic Stem Cells. Stem Cell Reports. 2015 May 28. pii: S2213-6711(15)00128-9. doi: 10.1016/j.stemcr.2015.04.016. PDX1-eGFP mini-vector donor for integration of the eGFP reporter into the PDX1 locus pBlueScript SKII (+) PDX1 GSF, IDX-1, IPF1, IUF1, MODY4, PAGEN1, PDX-1, STF-1 Add to Cart
66986OCT4-2A-mOrange2A-mOrangeA CRISPR/Cas-Mediated Selection-free Knockin Strategy in Human Embryonic Stem Cells. Stem Cell Reports. 2015 May 28. pii: S2213-6711(15)00128-9. doi: 10.1016/j.stemcr.2015.04.016. Generate OCT4 mOrange reporter line without selection cassette OCT4-2A-eGFP-PGKPuro plasmid (Addgene: 31938) Add to Cart
66989Oct4-Cr1gRNA for Crispr targeting the OCT4 stop codon (Homo sapiens)Mammalian ExpressionA CRISPR/Cas-Mediated Selection-free Knockin Strategy in Human Embryonic Stem Cells. Stem Cell Reports. 2015 May 28. pii: S2213-6711(15)00128-9. doi: 10.1016/j.stemcr.2015.04.016. Expresses gRNA that targets stop codon for OCT4 piCRg Entry (Addgene plasmid #58904) Add to Cart
75336Puro-iDESTTRE-DEST (Other)Mammalian ExpressionGenome Editing of Lineage Determinants in Human Pluripotent Stem Cells Reveals Mechanisms of Pancreatic Development and Diabetes. Cell Stem Cell. 2016 Apr 27. pii: S1934-5909(16)30002-9. doi: 10.1016/j.stem.2016.03.015. GATEWAY destination vector for site-specific integration into the human AAVS1/PPP1R12C locus for doxycycline-inducible gene over-expression N/A Add to Cart
75337Puro-iNgn3neurogenin 3 (Mus musculus)Mammalian ExpressionGenome Editing of Lineage Determinants in Human Pluripotent Stem Cells Reveals Mechanisms of Pancreatic Development and Diabetes. Cell Stem Cell. 2016 Apr 27. pii: S1934-5909(16)30002-9. doi: 10.1016/j.stem.2016.03.015. doxycycline inducible Ngn3 over-expression N/A Neurog3 Atoh5, Math4B, bHLHa7, ngn3 Add to Cart
75338Puro-iNotch1ICNotch1 Intracellular domain (Mus musculus)Mammalian ExpressionGenome Editing of Lineage Determinants in Human Pluripotent Stem Cells Reveals Mechanisms of Pancreatic Development and Diabetes. Cell Stem Cell. 2016 Apr 27. pii: S1934-5909(16)30002-9. doi: 10.1016/j.stem.2016.03.015. doxycycline inducible Notch1 intracellular domain gene over-expression N/A Notch1 9930111A19Rik, Mis6, N1, Tan1, lin-12 Add to Cart
75339Hygro-iDESTTRE-DEST (Other)Mammalian ExpressionGenome Editing of Lineage Determinants in Human Pluripotent Stem Cells Reveals Mechanisms of Pancreatic Development and Diabetes. Cell Stem Cell. 2016 Apr 27. pii: S1934-5909(16)30002-9. doi: 10.1016/j.stem.2016.03.015. GATEWAY destination vector for site-specific integration into the human AAVS1/PPP1R12C locus for doxycycline-inducible gene over-expression N/A Add to Cart
75340Hygro-iNGN3NGN3 (Homo sapiens)Mammalian ExpressionGenome Editing of Lineage Determinants in Human Pluripotent Stem Cells Reveals Mechanisms of Pancreatic Development and Diabetes. Cell Stem Cell. 2016 Apr 27. pii: S1934-5909(16)30002-9. doi: 10.1016/j.stem.2016.03.015. doxycycline inducible NGN3 gene over-expression N/A Add to Cart
86883Hygro-Cas9 donorhSpCas9 (Other)Mammalian Expression, CRISPRGenome Editing in hPSCs Reveals GATA6 Haploinsufficiency and a Genetic Interaction with GATA4 in Human Pancreatic Development. Cell Stem Cell. 2017 Feb 8. pii: S1934-5909(17)30001-2. doi: 10.1016/j.stem.2017.01.001. Donor vector for genomic targeting of a Tetracycline-inducible Cas9 cassette to the human AAVS1/PPP1R12C locus with hygromycin selection N/A Add to Cart