David Baker Lab Plasmids


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Title Authors Publication
Principles for designing ideal protein structures. Koga N, Tatsumi-Koga R, Liu G, Xiao R, Acton TB, Montelione GT, Baker D Nature. 2012 Nov 8;491(7423):222-7. doi: 10.1038/nature11600.
Community-Wide Assessment of Protein-Interface Modeling Suggests Improvements to Design Methodology. Fleishman SJ, Whitehead TA, Strauch EM, Corn JE, Qin S, Zhou HX, Mitchell JC, Demerdash ON, Takeda-Shitaka M, Terashi G, Moal IH, Li X, Bates PA, Zacharias M, Park H, Ko JS, Lee H, Seok C, Bourquard T, Bernauer J, Poupon A, Aze J, Soner S, Ovali SK, Ozbek P, Tal NB, Haliloglu T, Hwang H, Vreven T, Pierce BG, Weng Z, Perez-Cano L, Pons C, Fernandez-Recio J, Jiang F, Yang F, Gong X, Cao L, Xu X, Liu B, Wang P, Li C, Wang C, Robert CH, Guharoy M, Liu S, Huang Y, Li L, Guo D, Chen Y, Xiao Y, London N, Itzhaki Z, Schueler-Furman O, Inbar Y, Patapov V, Cohen M, Schreiber G, Tsuchiya Y, Kanamori E, Standley DM, Nakamura H, Kinoshita K, Driggers CM, Hall RG, Morgan JL, Hsu VL, Zhan J, Yang Y, Zhou Y, Kastritis PL, Bonvin AM, Zhang W, Camacho CJ, Kilambi KP, Sircar A, Gray JJ, Ohue M, Uchikoga N, Matsuzaki Y, Ishida T, Akiyama Y, Khashan R, Bush S, Fouches D, Tropsha A, Esquivel-Rodriguez J, Kihara D, Stranges PB, Jacak R, Kuhlman B, Huang SY, Zou X, Wodak SJ, Janin J, Baker D J Mol Biol. 2011 Sep 29.
A de novo protein binding pair by computational design and directed evolution. Karanicolas J, Corn JE, Chen I, Joachimiak LA, Dym O, Peck SH, Albeck S, Unger T, Hu W, Liu G, Delbecq S, Montelione GT, Spiegel CP, Liu DR, Baker D Mol Cell. 2011 Apr 22;42(2):250-60. Epub 2011 Mar 31.
Design of a novel globular protein fold with atomic-level accuracy. Kuhlman B, Dantas G, Ireton GC, Varani G, Stoddard BL, Baker D Science. 2003 Nov 21. 302(5649):1364-8.
A large scale test of computational protein design: folding and stability of nine completely redesigned globular proteins. Dantas G, Kuhlman B, Callender D, Wong M, Baker D J Mol Biol. 2003 Sep 12. 332(2):449-60.
ferre_variation_Amanda (unpublished)
Mutations_Koga_Nature (unpublished)
Expression plasmids for designed HEL-binding proteins (unpublished)
Other David Baker Plasmids (unpublished)
Top7 plasmids (unpublished)
ID Plasmid Gene/Insert Vector Type Publication Hidden Extra Search Info  
12393 Top7 V48V Top7 V48V Bacterial Expression Kuhlman et al Science. 2003 Nov 21. 302(5649):1364-8. pet29 b(+)
12394 PL1 PL1 Bacterial Expression Dantas et al J Mol Biol. 2003 Sep 12. 332(2):449-60. pet29 b(+)
12395 PL2 PL2 Bacterial Expression Dantas et al J Mol Biol. 2003 Sep 12. 332(2):449-60. pet29 b(+)
12396 ACY ACY Bacterial Expression Dantas et al J Mol Biol. 2003 Sep 12. 332(2):449-60. pet29 b(+)
12398 Top7 F15Q Y17L V48V Top7 F15Q Y17L V48V Bacterial Expression Top7 plasmids (unpublished) pet29 b(+)
12399 Top7 V48V K55E F61A Top7 V48V K55E F61A Bacterial Expression Top7 plasmids (unpublished) pet29 b(+)
12400 Top7 Y17L K39E K40E V48V K55E Top7 Y19L K39E K40E V48V K55E Bacterial Expression Top7 plasmids (unpublished) pet29 b(+)
12464 Top7 K39E K40E V48V K55E Top7 K39E K40E V48V K55E Bacterial Expression Top7 plasmids (unpublished) pet29 b(+)
12465 Top7 K39E K40E V48V K55E V91A Top7 K39E K40E V48V K55E V91A Bacterial Expression Top7 plasmids (unpublished) pet29 b(+)
12466 Top7 K39E K40E V48V K55E V82A Top7 K39E K40E V48V K55E V82A Bacterial Expression Top7 plasmids (unpublished) pet29 b(+)
12467 Top7 N32G K39E K40E V48V K55E Top7 N32G K39E K40E V48V K55E Bacterial Expression Top7 plasmids (unpublished) pet29 b(+)
12468 Top7 L27A K39E K40E V48V K55E Top7 L27A K39E K40E V48V K55E Bacterial Expression Top7 plasmids (unpublished) pet29 b(+)
12469 Top7 K39E K40E V48V K55E G86A Top7 K39E K40E V48V K55E G86A Bacterial Expression Top7 plasmids (unpublished) pet29 b(+)
12470 Top7 V30A K39E K40E V48V K55E Top7 V30A K39E K40E V48V K55E Bacterial Expression Top7 plasmids (unpublished) pet29 b(+)
12471 Top7 K39E K40E V48V K55E L75A Top7 K39E K40E V48V K55E L75A Bacterial Expression Top7 plasmids (unpublished) pet29 b(+)
12472 Top7 F15Q Y17L K39E K40E V48V K55E Top7 F17Q Y17L K39E K40E V48V K55E Bacterial Expression Top7 plasmids (unpublished) pet29 b(+)
12473 Top7 D9T D10V N14T Top7 D9T D10V N14T Bacterial Expression Top7 plasmids (unpublished) pet29 b(+)
12474 Top7 N7K D9T D10V N14T F15Y D16E Y17I Y37L K39E K40E Q41L Top7 N7K D9T D10V N14T F15Y D16E Y17I Y37L K39E K40E Q41L Bacterial Expression Top7 plasmids (unpublished) pet29 b(+)
12475 Top7 Eb13 triple Top7 Eb13 triple Bacterial Expression Top7 plasmids (unpublished) pet29 b(+)
12476 Top7 Eb8_2 triple Top7 Eb8_2 triple Bacterial Expression Top7 plasmids (unpublished) pet29 b(+)
12477 Top7 S2-H2 Top7 S2-H2 Bacterial Expression Top7 plasmids (unpublished) pet29 b(+)
12478 Top7 S2-S4 Top7 S2-S4 Bacterial Expression Top7 plasmids (unpublished) pet29 b(+)
12479 Top7 H1-H2 Top7 H1-H2 Bacterial Expression Top7 plasmids (unpublished) pet29 b(+)
12480 Top7 H1-S4 Top7 H1-S4 Bacterial Expression Top7 plasmids (unpublished) pet29 b(+)
12481 ssCFr ssCFr Bacterial Expression Other David Baker Plasmids (unpublished) pet29 b(+)
12482 CFr CFr Bacterial Expression Other David Baker Plasmids (unpublished) pet29 b(+)
12483 VJQ_Nterm VJQ Nterm Bacterial Expression Other David Baker Plasmids (unpublished) pet29 b(+)
12484 AYE_Nterm AYE Nterm Bacterial Expression Dantas et al J Mol Biol. 2003 Sep 12. 332(2):449-60. pet29 b(+)
12485 TOP8 Top8 Bacterial Expression Other David Baker Plasmids (unpublished) pet29 b(+)
12498 TEN TEN Bacterial Expression Dantas et al J Mol Biol. 2003 Sep 12. 332(2):449-60. pet29 b(+)
12499 FKB FKB Bacterial Expression Dantas et al J Mol Biol. 2003 Sep 12. 332(2):449-60. pet29 b(+)
12502 LMB LMB Bacterial Expression Dantas et al J Mol Biol. 2003 Sep 12. 332(2):449-60. pet29 b(+)
24785 Prb Prb Bacterial Expression Karanicolas et al Mol Cell. 2011 Apr 22;42(2):250-60. Epub 2011 Mar 31. pET29
24786 Pdar Pdar Bacterial Expression Karanicolas et al Mol Cell. 2011 Apr 22;42(2):250-60. Epub 2011 Mar 31. pET29
24787 PrbC5 Prb Bacterial Expression Karanicolas et al Mol Cell. 2011 Apr 22;42(2):250-60. Epub 2011 Mar 31. pET29LIC
24788 PrbC10 Prb Bacterial Expression Karanicolas et al Mol Cell. 2011 Apr 22;42(2):250-60. Epub 2011 Mar 31. pET29LIC
24830 PH1109 PH1109 Bacterial Expression Karanicolas et al Mol Cell. 2011 Apr 22;42(2):250-60. Epub 2011 Mar 31. pET29 PH1109
24833 Prb(Asp83Asn) Prb(Asp83Asn) Bacterial Expression Karanicolas et al Mol Cell. 2011 Apr 22;42(2):250-60. Epub 2011 Mar 31. pET
24834 Pdar(Asn34Asp) Pdar Asn34Asp Mammalian Expression Karanicolas et al Mol Cell. 2011 Apr 22;42(2):250-60. Epub 2011 Mar 31. pCTCON2
24835 PdarC1 PdarC1 Yeast Expression Karanicolas et al Mol Cell. 2011 Apr 22;42(2):250-60. Epub 2011 Mar 31. pCTCON2
24893 sankE3_5 sank E3_5 Bacterial Expression Karanicolas et al Mol Cell. 2011 Apr 22;42(2):250-60. Epub 2011 Mar 31. pET29
30229 pETCON-E32/design_15 Mtb ACP2 designed binding protein E32 Yeast Expression Fleishman et al J Mol Biol. 2011 Sep 29. pETCON
30230 pETCON-E34/design_51 Mtb ACP2 designed binding protein E34 Yeast Expression Fleishman et al J Mol Biol. 2011 Sep 29. pETCON
30231 pETCON-E36/design_49 Mtb ACP2 designed binding protein E36 Yeast Expression Fleishman et al J Mol Biol. 2011 Sep 29. pETCON
30232 pETCON-E37/design_71 Mtb ACP2 designed binding protein E37 Yeast Expression Fleishman et al J Mol Biol. 2011 Sep 29. pETCON
30233 pETCON-E39/design_28 Mtb ACP2 designed binding protein E39 Yeast Expression Fleishman et al J Mol Biol. 2011 Sep 29. pETCON
30234 pETCON-E40/design_68 Mtb ACP2 designed binding protein E40 Yeast Expression Fleishman et al J Mol Biol. 2011 Sep 29. pETCON
30235 pETCON-E41/design_8 Mtb ACP2 designed binding protein E41 Yeast Expression Fleishman et al J Mol Biol. 2011 Sep 29. pETCON
30236 pETCON-E42/design_37 Mtb ACP2 designed binding protein E42 Yeast Expression Fleishman et al J Mol Biol. 2011 Sep 29. pETCON
30237 pETCON-E43/design_55 Mtb ACP2 designed binding protein E43 Yeast Expression Fleishman et al J Mol Biol. 2011 Sep 29. pETCON
30238 pETCON-E45/design_33 Mtb ACP2 designed binding protein E45 Yeast Expression Fleishman et al J Mol Biol. 2011 Sep 29. pETCON
30239 pETCON-E47/design_20 Mtb ACP2 designed binding protein E47 Yeast Expression Fleishman et al J Mol Biol. 2011 Sep 29. pETCON
30241 pETCON-E49/design_82 Mtb ACP2 designed binding protein E49 Yeast Expression Fleishman et al J Mol Biol. 2011 Sep 29. pETCON
30242 pETCON-E51/design_83 Mtb ACP2 designed binding protein E51 Yeast Expression Fleishman et al J Mol Biol. 2011 Sep 29. pETCON
30243 pETCON-E54/design_29 Mtb ACP2 designed binding protein E54 Yeast Expression Fleishman et al J Mol Biol. 2011 Sep 29. pETCON
30244 pETCON-E59/design_75 Mtb ACP2 designed binding protein E59 Yeast Expression Fleishman et al J Mol Biol. 2011 Sep 29. pETCON
30245 pETCON-E62/design_19 Mtb ACP2 designed binding protein E62 Yeast Expression Fleishman et al J Mol Biol. 2011 Sep 29. pETCON
30246 pETCON-E64/design_38 Mtb ACP2 designed binding protein E64 Yeast Expression Fleishman et al J Mol Biol. 2011 Sep 29. pETCON
30247 pETCON-E66/design_62 Mtb ACP2 designed binding protein E66 Yeast Expression Fleishman et al J Mol Biol. 2011 Sep 29. pETCON
30249 pETCON-HB14/design_53 SC1918/H1 HA designed binding protein HB14 Yeast Expression Fleishman et al J Mol Biol. 2011 Sep 29. pETCON
30250 pETCON-HB15/design_17 SC1918/H1 HA designed binding protein HB15 Yeast Expression Fleishman et al J Mol Biol. 2011 Sep 29. pETCON
30251 pETCON-HB16/design_76 SC1918/H1 HA designed binding protein HB16 Yeast Expression Fleishman et al J Mol Biol. 2011 Sep 29. pETCON
30253 pETCON-HB19/design_63 SC1918/H1 HA designed binding protein HB19 Yeast Expression Fleishman et al J Mol Biol. 2011 Sep 29. pETCON
30254 pETCON-HB20/design_70 SC1918/H1 HA designed binding protein HB20 Yeast Expression Fleishman et al J Mol Biol. 2011 Sep 29. pETCON
30255 pETCON-HB1/design_36 SC1918/H1 HA designed binding protein HB1 Yeast Expression Fleishman et al J Mol Biol. 2011 Sep 29. pETCON
30256 pETCON-HB21/design_48 SC1918/H1 HA designed binding protein HB21 Yeast Expression Fleishman et al J Mol Biol. 2011 Sep 29. pETCON
30257 pETCON-HB22/design_4 SC1918/H1 HA designed binding protein HB22 Yeast Expression Fleishman et al J Mol Biol. 2011 Sep 29. pETCON
30258 pETCON-HB23/design_64 SC1918/H1 HA designed binding protein HB23 Yeast Expression Fleishman et al J Mol Biol. 2011 Sep 29. pETCON
30259 pETCON-HB27/design_66 SC1918/H1 HA designed binding protein HB27 Yeast Expression Fleishman et al J Mol Biol. 2011 Sep 29. pETCON
30260 pETCON-HB28/design_25 SC1918/H1 HA designed binding protein HB28 Yeast Expression Fleishman et al J Mol Biol. 2011 Sep 29. pETCON
30261 pETCON-HB30/design_65 SC1918/H1 HA designed binding protein HB30 Yeast Expression Fleishman et al J Mol Biol. 2011 Sep 29. pETCON
30262 pETCON-HB31/design_69 SC1918/H1 HA designed binding protein HB31 Yeast Expression Fleishman et al J Mol Biol. 2011 Sep 29. pETCON
30263 pETCON-HB32/design_46 SC1918/H1 HA designed binding protein HB32 Yeast Expression Fleishman et al J Mol Biol. 2011 Sep 29. pETCON
30264 pETCON-HB37/design_87 SC1918/H1 HA designed binding protein HB37 Yeast Expression Fleishman et al J Mol Biol. 2011 Sep 29. pETCON
30265 pETCON-HB39/design_12 SC1918/H1 HA designed binding protein HB39 Yeast Expression Fleishman et al J Mol Biol. 2011 Sep 29. pETCON
30266 pETCON-HB3/design_86 SC1918/H1 HA designed binding protein HB3 Yeast Expression Fleishman et al J Mol Biol. 2011 Sep 29. pETCON
30267 pETCON-HB43/design_1 SC1918/H1 HA designed binding protein HB43 Yeast Expression Fleishman et al J Mol Biol. 2011 Sep 29. pETCON
30268 pETCON-HB44/design_80 SC1918/H1 HA designed binding protein HB44 Yeast Expression Fleishman et al J Mol Biol. 2011 Sep 29. pETCON
30269 pETCON-HB48/design_73 SC1918/H1 HA designed binding protein HB48 Yeast Expression Fleishman et al J Mol Biol. 2011 Sep 29. pETCON
30270 pETCON-HB50/design_43 SC1918/H1 HA designed binding protein HB50 Yeast Expression Fleishman et al J Mol Biol. 2011 Sep 29. pETCON
30271 pETCON-HB4/design_2 SC1918/H1 HA designed binding protein HB4 Yeast Expression Fleishman et al J Mol Biol. 2011 Sep 29. pETCON
30272 pETCON-HB51/design_77 SC1918/H1 HA designed binding protein HB51 Yeast Expression Fleishman et al J Mol Biol. 2011 Sep 29. pETCON
30273 pETCON-HB53/design_9 SC1918/H1 HA designed binding protein HB53 Yeast Expression Fleishman et al J Mol Biol. 2011 Sep 29. pETCON
30274 pETCON-HB54/design_7 SC1918/H1 HA designed binding protein HB54 Yeast Expression Fleishman et al J Mol Biol. 2011 Sep 29. pETCON
30275 pETCON-HB55/design_85 SC1918/H1 HA designed binding protein HB55 Yeast Expression Fleishman et al J Mol Biol. 2011 Sep 29. pETCON
30276 pETCON-HB56/design_34 SC1918/H1 HA designed binding protein HB56 Yeast Expression Fleishman et al J Mol Biol. 2011 Sep 29. pETCON
30277 pETCON-HB58/design_26 SC1918/H1 HA designed binding protein HB58 Yeast Expression Fleishman et al J Mol Biol. 2011 Sep 29. pETCON
30278 pETCON-HB5/design_58 SC1918/H1 HA designed binding protein HB5 Yeast Expression Fleishman et al J Mol Biol. 2011 Sep 29. pETCON
30279 pETCON-HB62/design_5 SC1918/H1 HA designed binding protein HB62 Yeast Expression Fleishman et al J Mol Biol. 2011 Sep 29. pETCON
30280 pETCON-HB63/design_11 SC1918/H1 HA designed binding protein HB63 Yeast Expression Fleishman et al J Mol Biol. 2011 Sep 29. pETCON
30281 pETCON-HB64/design_81 SC1918/H1 HA designed binding protein HB64 Yeast Expression Fleishman et al J Mol Biol. 2011 Sep 29. pETCON
30282 pETCON-HB65/design_72 SC1918/H1 HA designed binding protein HB65 Yeast Expression Fleishman et al J Mol Biol. 2011 Sep 29. pETCON
30283 pETCON-HB67/design_32 SC1918/H1 HA designed binding protein HB67 Yeast Expression Fleishman et al J Mol Biol. 2011 Sep 29. pETCON
30284 pETCON-HB68/design_60 SC1918/H1 HA designed binding protein HB68 Yeast Expression Fleishman et al J Mol Biol. 2011 Sep 29. pETCON
30285 pETCON-HB69/design_61 SC1918/H1 HA designed binding protein HB69 Yeast Expression Fleishman et al J Mol Biol. 2011 Sep 29. pETCON
30286 pETCON-HB70/design_27 SC1918/H1 HA designed binding protein HB70 Yeast Expression Fleishman et al J Mol Biol. 2011 Sep 29. pETCON
30287 pETCON-HB6/design_16 SC1918/H1 HA designed binding protein HB6 Yeast Expression Fleishman et al J Mol Biol. 2011 Sep 29. pETCON
30288 pETCON-HB71/design_10 SC1918/H1 HA designed binding protein HB71 Yeast Expression Fleishman et al J Mol Biol. 2011 Sep 29. pETCON
30289 pETCON-HB72/design_31 SC1918/H1 HA designed binding protein HB72 Yeast Expression Fleishman et al J Mol Biol. 2011 Sep 29. pETCON
30290 pETCON-HB73/design_74 SC1918/H1 HA designed binding protein HB73 Yeast Expression Fleishman et al J Mol Biol. 2011 Sep 29. pETCON
30291 pETCON-HB74/design_56 SC1918/H1 HA designed binding protein HB74 Yeast Expression Fleishman et al J Mol Biol. 2011 Sep 29. pETCON
30292 pETCON-HB75/design_18 SC1918/H1 HA designed binding protein HB75 Yeast Expression Fleishman et al J Mol Biol. 2011 Sep 29. pETCON
30293 pETCON-HB76/design_13 SC1918/H1 HA designed binding protein HB76 Yeast Expression Fleishman et al J Mol Biol. 2011 Sep 29. pETCON
30294 pETCON-HB77/design_47 SC1918/H1 HA designed binding protein HB77 Yeast Expression Fleishman et al J Mol Biol. 2011 Sep 29. pETCON
30295 pETCON-HB78/design_44 SC1918/H1 HA designed binding protein HB78 Yeast Expression Fleishman et al J Mol Biol. 2011 Sep 29. pETCON
30297 pETCON-HB81/design_35 SC1918/H1 HA designed binding protein HB81 Yeast Expression Fleishman et al J Mol Biol. 2011 Sep 29. pETCON
30298 pETCON-HB82/design_21 SC1918/H1 HA designed binding protein HB82 Yeast Expression Fleishman et al J Mol Biol. 2011 Sep 29. pETCON
30299 pETCON-HB7/design_52 SC1918/H1 HA designed binding protein HB7 Yeast Expression Fleishman et al J Mol Biol. 2011 Sep 29. pETCON
30300 pETCON-HB9/design_41 SC1918/H1 HA designed binding protein HB9 Yeast Expression Fleishman et al J Mol Biol. 2011 Sep 29. pETCON
30301 pETCON-IgG1/design_50 IgG Fc Designed Binder IgG1 Yeast Expression Fleishman et al J Mol Biol. 2011 Sep 29. pETCON
30302 pETCON-IgG3/design_30 IgG Fc Designed Binder IgG3 Yeast Expression Fleishman et al J Mol Biol. 2011 Sep 29. pETCON
30303 pETCON-IgG9/design_59 IgG Fc Designed Binder IgG9 Yeast Expression Fleishman et al J Mol Biol. 2011 Sep 29. pETCON
45120 pETCON-HtsptLB12 HtsptLB12 (Synthetic) Yeast Expression Expression plasmids for designed HEL-binding proteins (unpublished) pETCON
45121 pETCON-HtsptLB12v.3 HtsptLB12v.3 (Synthetic) Yeast Expression Expression plasmids for designed HEL-binding proteins (unpublished) pETCON
45122 pET29b-HtsptLB12 HtsptLB12 (Synthetic) Bacterial Expression Expression plasmids for designed HEL-binding proteins (unpublished) pET29b(+)
45123 pET29b-HtsptLB12v.3 HtsptLB12v.3 (Synthetic) Bacterial Expression Expression plasmids for designed HEL-binding proteins (unpublished) pET29b(+)
  • Tag / Fusion Protein
    • 6xHis (C terminal on backbone)
  • 45124 pETCON-DnvLB16 DnvLB16 (Synthetic) Yeast Expression Expression plasmids for designed HEL-binding proteins (unpublished) pETCON
    45125 pETCON-DnvLB16 M40R;W41H;H42S DnvLB16 M40R;W41H;H42S (Synthetic) Yeast Expression Expression plasmids for designed HEL-binding proteins (unpublished) pETCON
    45484 Di-I_5_FR28 Di-I_5_FR28 (Synthetic) Bacterial Expression Koga et al Nature. 2012 Nov 8;491(7423):222-7. doi: 10.1038/nature11600. pET29b(+)
    45485 Di-II_10_R2x2_769 Di-II_10_R2x2_769 (Synthetic) Bacterial Expression Koga et al Nature. 2012 Nov 8;491(7423):222-7. doi: 10.1038/nature11600. pET29b(+)
    45486 Di-III_14_IF3_14 Di-III_14_IF3_14 (Synthetic) Bacterial Expression Koga et al Nature. 2012 Nov 8;491(7423):222-7. doi: 10.1038/nature11600. pET29b(+)
    45487 Di-IV_5_PL2x2_6 Di-IV_5_PL2x2_6 (Synthetic) Bacterial Expression Koga et al Nature. 2012 Nov 8;491(7423):222-7. doi: 10.1038/nature11600. pET29b(+)
    45488 Di-V_7_R3x1_7 Di-V_7_R3x1_7 (Synthetic) Bacterial Expression Koga et al Nature. 2012 Nov 8;491(7423):222-7. doi: 10.1038/nature11600. pET29b(+)
    45489 FR52re FR52re (Synthetic) Bacterial Expression Mutations_Koga_Nature (unpublished) pET29b(+)
    45490 FR55 FR55 (Synthetic) Bacterial Expression Mutations_Koga_Nature (unpublished) pET29b(+)
    45491 PL2x2_5 PL2x2_5 (Synthetic) Bacterial Expression Mutations_Koga_Nature (unpublished) pET29b(+)
    45606 FDA60 FDA60 (Synthetic) Bacterial Expression ferre_variation_Amanda (unpublished) peT21b(+)