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David McKay Lab Plasmids


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Title Authors Publication
Destabilization of peptide binding and interdomain communication by an E543K mutation in the bovine 70-kDa heat shock cognate protein, a molecular chaperone. Ha JH, Hellman U, Johnson ER, Li L, McKay DB, Sousa MC, Takeda S, Wernstedt C, Wilbanks SM J Biol Chem. 1997 Oct 31. 272(44):27796-803.
Crystal and solution structures of an HslUV protease-chaperone complex. Sousa MC, Trame CB, Tsuruta H, Wilbanks SM, Reddy VS, McKay DB Cell. 2000 Nov 10. 103(4):633-43.
Structure of Haemophilus influenzae HslU protein in crystals with one-dimensional disorder twinning. Trame CB, McKay DB Acta Crystallogr D Biol Crystallogr. 2001 Aug . 57(Pt 8):1079-90.
Crystallographic structure of SurA, a molecular chaperone that facilitates folding of outer membrane porins. Bitto E, McKay DB Structure. 2002 Nov . 10(11):1489-98.
The periplasmic molecular chaperone protein SurA binds a peptide motif that is characteristic of integral outer membrane proteins. Bitto E, McKay DB J Biol Chem. 2003 Dec 5. 278(49):49316-22.
YxiN is a modular protein combining a DEx(D/H) core and a specific RNA-binding domain. Karginov FV, Caruthers JM, Hu Y, McKay DB, Uhlenbeck OC J Biol Chem. 2005 Oct 21. 280(42):35499-505.
Structure of the second domain of the Bacillus subtilis DEAD-box RNA helicase YxiN. Caruthers JM, Hu Y, McKay DB Acta Crystallograph Sect F Struct Biol Cryst Commun. 2006 Dec 1. 62(Pt 12):1191-5.
The Periplasmic Bacterial Molecular Chaperone SurA Adapts its Structure to Bind Peptides in Different Conformations to Assert a Sequence Preference for Aromatic Residues. Xu X, Wang S, Hu YX, McKay DB J Mol Biol. 2007 Oct 19. 373(2):367-81.
The Bacillus subtilis RNA helicase YxiN is distended in solution. Wang S, Overgaard MT, Hu Y, McKay DB Biophys J. 2008 Jan 1. 94(1):L01-3.
Structure and reactivity of an asymmetric complex between HslV and I-domain deleted HslU, a prokaryotic homolog of the eukaryotic proteasome. Kwon AR, Kessler BM, Overkleeft HS, McKay DB J Mol Biol. 2003 Jul 4. 330(2):185-95.
Retention of core catalytic functions by a conserved minimal ribonuclease E peptide that lacks the domain required for tetramer formation. Caruthers JM, Feng Y, McKay DB, Cohen SN J Biol Chem. 2006 Sep 15. 281(37):27046-51.
Plasmids for expression of wt and E258Q mutant HslU (unpublished)
Structure of the RNA Binding Domain of a DEAD-Box Helicase Bound to Its Ribosomal RNA Target Reveals a Novel Mode of Recognition by an RNA Recognition Motif. Hardin JW, Hu YX, McKay DB J Mol Biol. 2010 Jul 29. ():.
Structural basis of the 70-kilodalton heat shock cognate protein ATP hydrolytic activity. I. Kinetic analyses of active site mutants. Wilbanks SM, DeLuca-Flaherty C, McKay DB J Biol Chem. 1994 Apr 29. 269(17):12893-8.
ID Plasmid Gene/Insert Vector Type Publication Hidden Extra Search Info  
12532Bov.hsc70.RSET.FL.wt(NarI)bovine 70 kDa heat shock cognate protein full length wild type (Bos taurus)Bacterial ExpressionDestabilization of peptide binding and interdomain communication by an E543K mutation in the bovine 70-kDa heat shock cognate protein, a molecular chaperone. J Biol Chem. 1997 Oct 31. 272(44):27796-803. pRSET_A Add to Cart
12533Bov.hsc70.RSET.a1-554.wt(NarI)Bovine 70 kDa heat shock cognate protein fragment amino acids 1-554 (Bos taurus)Bacterial ExpressionDestabilization of peptide binding and interdomain communication by an E543K mutation in the bovine 70-kDa heat shock cognate protein, a molecular chaperone. J Biol Chem. 1997 Oct 31. 272(44):27796-803. pRSET_A Add to Cart
12534Bov.hsc70.T77.A1-386.wtBovine 70 kDa heat shock protein ATPase fragment (Bos taurus)Bacterial ExpressionStructural basis of the 70-kilodalton heat shock cognate protein ATP hydrolytic activity. I. Kinetic analyses of active site mutants. J Biol Chem. 1994 Apr 29. 269(17):12893-8. pT7-7 Add to Cart
12538HI.HslUV.RSET.FL.wtHaemophilus influenzae heat shock loci U and VBacterial ExpressionCrystal and solution structures of an HslUV protease-chaperone complex. Cell. 2000 Nov 10. 103(4):633-43. pRSET_B Add to Cart
12556HI.HslU.RSETB.fl.wtHaemophilus influenzae heat shock locus UBacterial ExpressionStructure of Haemophilus influenzae HslU protein in crystals with one-dimensional disorder twinning. Acta Crystallogr D Biol Crystallogr. 2001 Aug . 57(Pt 8):1079-90. pRSET_B Add to Cart
12606EC.SurA.pTYB1.a21-428.wtEscherichia coli surABacterial ExpressionCrystallographic structure of SurA, a molecular chaperone that facilitates folding of outer membrane porins. Structure. 2002 Nov . 10(11):1489-98. pTYB1 surA b0053, ECK0054, JW0052 Add to Cart
12607EC.SurA.pTYB1.a21-281&390-428.wtEscherichia coli surA delta PPIase domain 2Bacterial ExpressionThe periplasmic molecular chaperone protein SurA binds a peptide motif that is characteristic of integral outer membrane proteins. J Biol Chem. 2003 Dec 5. 278(49):49316-22. pTYB1 Add to Cart
13527BS.YxiN.Twin1.a1-479.wtB. subtilis DEAD-box helicase YxiNBacterial ExpressionYxiN is a modular protein combining a DEx(D/H) core and a specific RNA-binding domain. J Biol Chem. 2005 Oct 21. 280(42):35499-505. pTWIN1 Add to Cart
13528BS.YxiN.Twin1.a1-368.wtB. subtilis DEAD-box helicase YxiN first two domainsBacterial ExpressionYxiN is a modular protein combining a DEx(D/H) core and a specific RNA-binding domain. J Biol Chem. 2005 Oct 21. 280(42):35499-505. pTWIN1 Add to Cart
13529BS.YxiN.Twin1.a404-479.wtB. subtilis DEAD-box helicase YxiN specific RNA binding domainBacterial ExpressionYxiN is a modular protein combining a DEx(D/H) core and a specific RNA-binding domain. J Biol Chem. 2005 Oct 21. 280(42):35499-505. pTWIN1 Add to Cart
13531BS.YxiN.Twin1.a207-368.wtB. subtilis DEAD-box helicase YxiN second domainBacterial ExpressionStructure of the second domain of the Bacillus subtilis DEAD-box RNA helicase YxiN. Acta Crystallograph Sect F Struct Biol Cryst Commun. 2006 Dec 1. 62(Pt 12):1191-5. pTWIN1 Add to Cart
15934EC.SurA.TYB1.a172-274.wtPeptidyl prolyl isomerase domain 1 of SurA (Other)Bacterial ExpressionThe Periplasmic Bacterial Molecular Chaperone SurA Adapts its Structure to Bind Peptides in Different Conformations to Assert a Sequence Preference for Aromatic Residues. J Mol Biol. 2007 Oct 19. 373(2):367-81. pTYB1 surA c0066 Add to Cart
15956EC.SurA.pTYB1.a21-428.M231Rmature SurA protein M231R mutant (Other)Bacterial ExpressionThe Periplasmic Bacterial Molecular Chaperone SurA Adapts its Structure to Bind Peptides in Different Conformations to Assert a Sequence Preference for Aromatic Residues. J Mol Biol. 2007 Oct 19. 373(2):367-81. pTYB1 Add to Cart
15957EC.SurA.pTYB1.a21-428.L239RsurA (Other)Bacterial ExpressionThe Periplasmic Bacterial Molecular Chaperone SurA Adapts its Structure to Bind Peptides in Different Conformations to Assert a Sequence Preference for Aromatic Residues. J Mol Biol. 2007 Oct 19. 373(2):367-81. pTYB1 surA c0066 Add to Cart
15958EC.SurA.pTYB1.a21-281&390-428.M231REscherichia coli surA delta PPIase domain 2 (Other)Bacterial ExpressionThe Periplasmic Bacterial Molecular Chaperone SurA Adapts its Structure to Bind Peptides in Different Conformations to Assert a Sequence Preference for Aromatic Residues. J Mol Biol. 2007 Oct 19. 373(2):367-81. pTYB1 Add to Cart
15959EC.SurA.pTYB1.a21-281&390-428.L239REscherichia coli surA delta PPIase domain 2 (Other)Bacterial ExpressionThe Periplasmic Bacterial Molecular Chaperone SurA Adapts its Structure to Bind Peptides in Different Conformations to Assert a Sequence Preference for Aromatic Residues. J Mol Biol. 2007 Oct 19. 373(2):367-81. pTYB1 Add to Cart
17516BS.YxiN.Twin1.a207-479.wtB. subtilis DEAD-box helicase YxiN second domainBacterial ExpressionThe Bacillus subtilis RNA helicase YxiN is distended in solution. Biophys J. 2008 Jan 1. 94(1):L01-3. pTWIN1 Add to Cart
17517BS.YxiN.Twin1.a1-368.E152QB. subtilis DEAD-box helicase YxiN first two domainsBacterial ExpressionThe Bacillus subtilis RNA helicase YxiN is distended in solution. Biophys J. 2008 Jan 1. 94(1):L01-3. pTWIN1 Add to Cart
17518BS.YxiN.Twin1.a1-479.E152QB. subtilis DEAD-box helicase YxiNBacterial ExpressionThe Bacillus subtilis RNA helicase YxiN is distended in solution. Biophys J. 2008 Jan 1. 94(1):L01-3. pTWIN1 Add to Cart
17859HI.HslU(delI)V.RSET.wt(Nde1).107-GG-244Haemophilus influenzae heat shock loci U and VBacterial ExpressionStructure and reactivity of an asymmetric complex between HslV and I-domain deleted HslU, a prokaryotic homolog of the eukaryotic proteasome. J Mol Biol. 2003 Jul 4. 330(2):185-95. pRSET_B Add to Cart
17949EC.RNe.TYB1.a1-400.wtEscherichia coli RNase E residues 1-400Bacterial ExpressionRetention of core catalytic functions by a conserved minimal ribonuclease E peptide that lacks the domain required for tetramer formation. J Biol Chem. 2006 Sep 15. 281(37):27046-51. pTYB1 Add to Cart
17950EC.RNe.TYB1.a1-395.wtEscherichia coli RNase E residues 1-395Bacterial ExpressionRetention of core catalytic functions by a conserved minimal ribonuclease E peptide that lacks the domain required for tetramer formation. J Biol Chem. 2006 Sep 15. 281(37):27046-51. pTYB1 Add to Cart
17961HI.HslUV.RSET.FL.wt(V).E258Q(U)Haemophilus influenzae heat shock loci U and VBacterial Expression Plasmids for expression of wt and E258Q mutant HslU (unpublished) pRSET_B Add to Cart
2619023SrRNA.RAV12(wt).2508-2580.G2530AAAC23S ribosomal RNA fragment (modified)Bacterial ExpressionStructure of the RNA Binding Domain of a DEAD-Box Helicase Bound to Its Ribosomal RNA Target Reveals a Novel Mode of Recognition by an RNA Recognition Motif. J Mol Biol. 2010 Jul 29. ():. modified pUC19 Add to Cart
2619123SrRNA.RAV12(wt).2508-2580.GAAAC.G2553U23S ribosomal RNA fragment (modified) with G2553U mutationBacterial ExpressionStructure of the RNA Binding Domain of a DEAD-Box Helicase Bound to Its Ribosomal RNA Target Reveals a Novel Mode of Recognition by an RNA Recognition Motif. J Mol Biol. 2010 Jul 29. ():. modified pUC19 Add to Cart
2619223SrRNA.RAV12(wt).2508-2580.GAAAC.duplexH9023S ribosomal RNA fragment (modified) with duplex H90Bacterial ExpressionStructure of the RNA Binding Domain of a DEAD-Box Helicase Bound to Its Ribosomal RNA Target Reveals a Novel Mode of Recognition by an RNA Recognition Motif. J Mol Biol. 2010 Jul 29. ():. modified pUC19 Add to Cart