Edward Chan Lab Plasmids


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Title Authors Publication
miR-375 activates p21 and suppresses telomerase activity by coordinately regulating HPV E6/E7, E6AP, CIP2A, and 14-3-3zeta. Jung HM, Phillips BL, Chan EK Mol Cancer. 2014 Apr 8;13(1):80. doi: 10.1186/1476-4598-13-80.
Tumor suppressor miR-375 regulates MYC expression via repression of CIP2A coding sequence through multiple miRNA-mRNA interactions. Jung HM, Patel RS, Phillips BL, Wang H, Cohen DM, Reinhold WC, Chang LJ, Yang LJ, Chan EK Mol Biol Cell. 2013 Jun;24(11):1638-48, S1-7. doi: 10.1091/mbc.E12-12-0891. Epub 2013 Apr 3.
Keratinization-associated miR-7 and miR-21 regulate tumor suppressor reversion-inducing cysteine-rich protein with kazal motifs (RECK) in oral cancer. Jung HM, Phillips BL, Patel RS, Cohen DM, Jakymiw A, Kong WW, Cheng JQ, Chan EK J Biol Chem. 2012 Aug 24;287(35):29261-72. doi: 10.1074/jbc.M112.366518. Epub 2012 Jul 2.
Divergent GW182 functional domains in the regulation of translational silencing. Yao B, Li S, Jung HM, Lian SL, Abadal GX, Han F, Fritzler MJ, Chan EK Nucleic Acids Res. 2011 Apr;39(7):2534-47. Epub 2010 Dec 2.
The C-terminal half of human Ago2 binds to multiple GW-rich regions of GW182 and requires GW182 to mediate silencing. Lian SL, Li S, Abadal GX, Pauley BA, Fritzler MJ, Chan EK RNA. 2009 May . 15(5):804-13.
Disruption of GW bodies impairs mammalian RNA interference. Jakymiw A, Lian S, Eystathioy T, Li S, Satoh M, Hamel JC, Fritzler MJ, Chan EK Nat Cell Biol. 2005 Dec . 7(12):1267-74.
ID Plasmid Gene/Insert Vector Type Publication Hidden Extra Search Info  
11590 GFP-hAgo2 Ago2 (Homo sapiens) Mammalian Expression Jakymiw et al Nat Cell Biol. 2005 Dec . 7(12):1267-74. custom-made pDEST:gfp vector AGO2 EIF2C2, Q10
11591 GFP-PIWI Ago2 (Homo sapiens) Mammalian Expression Jakymiw et al Nat Cell Biol. 2005 Dec . 7(12):1267-74. custom-made pDEST:gfp vector AGO2 EIF2C2, Q10
11592 GFP-GW182delta1 GW182 (Homo sapiens) Mammalian Expression Jakymiw et al Nat Cell Biol. 2005 Dec . 7(12):1267-74. custom-made pDEST:gfp vector TNRC6A CAGH26, GW1, GW182, TNRC6
21514 GST-TNR(aa1-204) TNRC6A (Homo sapiens) Mammalian Expression Lian et al RNA. 2009 May . 15(5):804-13. pCG-GST TNRC6A CAGH26, GW1, GW182, TNRC6
21516 GST-GW1D10(aa566-1343) TNRC6A (Homo sapiens) Mammalian Expression Lian et al RNA. 2009 May . 15(5):804-13. pCG-GST TNRC6A CAGH26, GW1, GW182, TNRC6
21517 GFP-GW1D10(aa566-1343) TNRC6A (Homo sapiens) Mammalian Expression Lian et al RNA. 2009 May . 15(5):804-13. custom-made pDEST:gfp vector TNRC6A CAGH26, GW1, GW182, TNRC6
21519 GFP-GW1D5(aa1670-1962) TNRC6A (Homo sapiens) Mammalian Expression Lian et al RNA. 2009 May . 15(5):804-13. custom-made pDEST:gfp vector TNRC6A CAGH26, GW1, GW182, TNRC6
21520 GFP-GW1D1a(aa254-503) TNRC6A (Homo sapiens) Mammalian Expression Lian et al RNA. 2009 May . 15(5):804-13. custom-made pDEST:gfp vector TNRC6A CAGH26, GW1, GW182, TNRC6
21521 GFP-GW1D1b(aa502-751) TNRC6A (Homo sapiens) Mammalian Expression Lian et al RNA. 2009 May . 15(5):804-13. custom-made pDEST:gfp vector TNRC6A CAGH26, GW1, GW182, TNRC6
21522 GFP-GW1D1aMut(W>A) TNRC6A (Homo sapiens) Mammalian Expression Lian et al RNA. 2009 May . 15(5):804-13. custom-made pDEST:gfp vector TNRC6A CAGH26, GW1, GW182, TNRC6
21523 GST-GW1D1(aa254-751) TNRC6A (Homo sapiens) Mammalian Expression Lian et al RNA. 2009 May . 15(5):804-13. pCG-GST TNRC6A CAGH26, GW1, GW182, TNRC6
21524 GFP-D1a_W1-2(aa270-346) TNRC6A (Homo sapiens) Mammalian Expression Lian et al RNA. 2009 May . 15(5):804-13. custom-made pDEST:gfp vector TNRC6A CAGH26, GW1, GW182, TNRC6
21525 GFP-D1a_W2-3(aa318-399) TNRC6A (Homo sapiens) Mammalian Expression Lian et al RNA. 2009 May . 15(5):804-13. custom-made pDEST:gfp vector TNRC6A CAGH26, GW1, GW182, TNRC6
21526 GFP-D1a_W3-4(aa340-439) TNRC6A (Homo sapiens) Mammalian Expression Lian et al RNA. 2009 May . 15(5):804-13. custom-made pDEST:gfp vector TNRC6A CAGH26, GW1, GW182, TNRC6
21527 GFP-D1a_W4-5(aa409-495) TNRC6A (Homo sapiens) Mammalian Expression Lian et al RNA. 2009 May . 15(5):804-13. custom-made pDEST:gfp vector TNRC6A CAGH26, GW1, GW182, TNRC6
21528 GST-GW1D12(aa895-1211) TNRC6A (Homo sapiens) Mammalian Expression Lian et al RNA. 2009 May . 15(5):804-13. pCG-GST TNRC6A CAGH26, GW1, GW182, TNRC6
21529 FLAG-PAZ(aa1-480) Ago2 (Homo sapiens) Mammalian Expression Lian et al RNA. 2009 May . 15(5):804-13. p3XFLAG-myc-CMV-24 AGO2 EIF2C2, Q10
21530 NHA-PAZ(aa1-480) Ago2 (Homo sapiens) Mammalian Expression Lian et al RNA. 2009 May . 15(5):804-13. pCl-neo_NHA AGO2 EIF2C2, Q10
21531 GFP-Ago3m Ago3 (Homo sapiens) Mammalian Expression Lian et al RNA. 2009 May . 15(5):804-13. custom-made pDEST:gfp vector AGO3 RP4-789D17.2, EIF2C3
21532 NHA-Ago3m Ago3 (Homo sapiens) Mammalian Expression Lian et al RNA. 2009 May . 15(5):804-13. pCl-neo_NHA AGO3 RP4-789D17.2, EIF2C3
21533 FLAG-Ago1 Ago1 (Homo sapiens) Mammalian Expression Lian et al RNA. 2009 May . 15(5):804-13. p3XFLAG-myc-CMV-24 AGO1 RP4-789D17.1, EIF2C, EIF2C1, GERP95, Q99
21534 GFP-Ago1 Ago1 (Homo sapiens) Mammalian Expression Lian et al RNA. 2009 May . 15(5):804-13. custom-made pDEST:gfp vector AGO1 RP4-789D17.1, EIF2C, EIF2C1, GERP95, Q99
21535 NHA-Ago1 Ago1 (Homo sapiens) Mammalian Expression Lian et al RNA. 2009 May . 15(5):804-13. pCl-neo_NHA AGO1 RP4-789D17.1, EIF2C, EIF2C1, GERP95, Q99
21536 GFP-Ago4 Ago4 (Homo sapiens) Mammalian Expression Lian et al RNA. 2009 May . 15(5):804-13. custom-made pDEST:gfp vector AGO4 EIF2C4
21537 NHA-Ago4 Ago4 (Homo sapiens) Mammalian Expression Lian et al RNA. 2009 May . 15(5):804-13. pCl-neo_NHA AGO4 EIF2C4
21538 FLAG-Ago2 Ago2 (Homo sapiens) Mammalian Expression Lian et al RNA. 2009 May . 15(5):804-13. p3XFLAG-myc-CMV-24 AGO2 EIF2C2, Q10
21541 NHA-PIWI(aa478-860) Ago2 (Homo sapiens) Mammalian Expression Lian et al RNA. 2009 May . 15(5):804-13. pCl-neo_NHA AGO2 EIF2C2, Q10
21542 GST-PIWI(aa478-860) Ago2 (Homo sapiens) Mammalian Expression Lian et al RNA. 2009 May . 15(5):804-13. pCG-GST AGO2 EIF2C2, Q10
21543 FLAG-PIWI(aa478-860) Ago2 (Homo sapiens) Mammalian Expression Lian et al RNA. 2009 May . 15(5):804-13. p3XFLAG-myc-CMV-24 AGO2 EIF2C2, Q10
36437 NHA-TNGW1 TNRC6A (Homo sapiens) Mammalian Expression Yao et al Nucleic Acids Res. 2011 Apr;39(7):2534-47. Epub 2010 Dec 2. pCl-neo_NHA TNRC6A CAGH26, GW1, GW182, TNRC6
36438 NHA-GW182 TNRC6A (Homo sapiens) Mammalian Expression Yao et al Nucleic Acids Res. 2011 Apr;39(7):2534-47. Epub 2010 Dec 2. pCl-neo_NHA TNRC6A CAGH26, GW1, GW182, TNRC6
36439 NHA-Delta 12 TNRC6A Delta 12 (Homo sapiens) Mammalian Expression Yao et al Nucleic Acids Res. 2011 Apr;39(7):2534-47. Epub 2010 Dec 2. pCl-neo_NHA TNRC6A CAGH26, GW1, GW182, TNRC6
36440 NHA- Delta 5 TNRC6A Delta 5 (Homo sapiens) Mammalian Expression Yao et al Nucleic Acids Res. 2011 Apr;39(7):2534-47. Epub 2010 Dec 2. pCl-neo_NHA TNRC6A CAGH26, GW1, GW182, TNRC6
36441 NHA-QN TNRC6A (Homo sapiens) Mammalian Expression Yao et al Nucleic Acids Res. 2011 Apr;39(7):2534-47. Epub 2010 Dec 2. pCl-neo_NHA TNRC6A CAGH26, GW1, GW182, TNRC6
36922 NHA-Ago2 Ago2 (Homo sapiens) Mammalian Expression Yao et al Nucleic Acids Res. 2011 Apr;39(7):2534-47. Epub 2010 Dec 2. pCl-neo_NHA AGO2 EIF2C2, Q10
36923 NHA-TNR TNGW1 (Homo sapiens) Mammalian Expression Yao et al Nucleic Acids Res. 2011 Apr;39(7):2534-47. Epub 2010 Dec 2. pCl-neo_NHA TNRC6A CAGH26, GW1, GW182, TNRC6
36925 NHA-Delta 10 TNGW1 (Homo sapiens) Mammalian Expression Yao et al Nucleic Acids Res. 2011 Apr;39(7):2534-47. Epub 2010 Dec 2. pCl-neo_NHA
36926 NHA-Delta 11 TNRC6A (Homo sapiens) Mammalian Expression Yao et al Nucleic Acids Res. 2011 Apr;39(7):2534-47. Epub 2010 Dec 2. pCl-neo_NHA TNRC6A CAGH26, GW1, GW182, TNRC6
36928 NHA-Delta 8 TNGW1 (Homo sapiens) Mammalian Expression Yao et al Nucleic Acids Res. 2011 Apr;39(7):2534-47. Epub 2010 Dec 2. pCl-neo_NHA TNRC6A CAGH26, GW1, GW182, TNRC6
36933 NHA-Delta 12a TNRC6A Delta 12a (Homo sapiens) Mammalian Expression Yao et al Nucleic Acids Res. 2011 Apr;39(7):2534-47. Epub 2010 Dec 2. pCl-neo_NHA TNRC6A CAGH26, GW1, GW182, TNRC6
36934 NHA-Delta 12b TNRC6A Delta 12b (Homo sapiens) Mammalian Expression Yao et al Nucleic Acids Res. 2011 Apr;39(7):2534-47. Epub 2010 Dec 2. pCl-neo_NHA TNRC6A CAGH26, GW1, GW182, TNRC6
36935 NHA-Delta 12am TNRC6A Delta 12am (Homo sapiens) Mammalian Expression Yao et al Nucleic Acids Res. 2011 Apr;39(7):2534-47. Epub 2010 Dec 2. pCl-neo_NHA TNRC6A CAGH26, GW1, GW182, TNRC6
36936 NHA-Delta 5a TNRC6A Delta 5a (Homo sapiens) Mammalian Expression Yao et al Nucleic Acids Res. 2011 Apr;39(7):2534-47. Epub 2010 Dec 2. pCl-neo_NHA TNRC6A CAGH26, GW1, GW182, TNRC6
36937 NHA-Delta 5b TNRC6A Delta 5b (Homo sapiens) Mammalian Expression Yao et al Nucleic Acids Res. 2011 Apr;39(7):2534-47. Epub 2010 Dec 2. pCl-neo_NHA TNRC6A CAGH26, GW1, GW182, TNRC6
53614 pMiR-RECK-3'UTR RECK-3'UTR (Homo sapiens) Luciferase Jung et al J Biol Chem. 2012 Aug 24;287(35):29261-72. doi: 10.1074/jbc.M112.366518. Epub 2012 Jul 2. Luciferase reporter assay for microRNA binding on the RECK 3'UTR pMiR-Target
53692 pMiR-RECK-3'UTR-miR-7-mut RECK-3'UTR (Homo sapiens) Luciferase Jung et al J Biol Chem. 2012 Aug 24;287(35):29261-72. doi: 10.1074/jbc.M112.366518. Epub 2012 Jul 2. Luciferase reporter assay for RECK 3'UTR that has point mutations on miR-7 binding site pMiR-Target
53693 pMiR-RECK-3'UTR-miR-21-mut RECK-3'UTR (Homo sapiens) Luciferase Jung et al J Biol Chem. 2012 Aug 24;287(35):29261-72. doi: 10.1074/jbc.M112.366518. Epub 2012 Jul 2. Luciferase reporter assay for RECK 3'UTR that has point mutations on miR-21 binding site pMiR-Target
53694 pMiR-E6AP-3'UTR UBE3A 3'UTR (Homo sapiens) Luciferase Jung et al Mol Cancer. 2014 Apr 8;13(1):80. doi: 10.1186/1476-4598-13-80. Luciferase reporter assay for microRNA binding on E6AP 3'UTR pMiR-Target UBE3A ANCR, AS, E6-AP, EPVE6AP, HPVE6A
53695 pMiR-E6AP-3'UTR-miR-375-mut UBE3A 3'UTR (Homo sapiens) Luciferase Jung et al Mol Cancer. 2014 Apr 8;13(1):80. doi: 10.1186/1476-4598-13-80. Luciferase reporter assay for E6AP 3'UTR with point mutation on miR-375 binding site pMiR-Target UBE3A ANCR, AS, E6-AP, EPVE6AP, HPVE6A
53696 pMiR-HPV16-E7 HPV16-E7 (Other) Luciferase Jung et al Mol Cancer. 2014 Apr 8;13(1):80. doi: 10.1186/1476-4598-13-80. Luciferase reporter assay for microRNA binding on HPV16 E7 pMiR-Target
53697 pMiR-HPV16-E7-m1 HPV16-E7 (Other) Luciferase Jung et al Mol Cancer. 2014 Apr 8;13(1):80. doi: 10.1186/1476-4598-13-80. Luciferase reporter assay for HPV16 E7 with point mutation on miR-375 binding site pMiR-Target
53698 pMiR-HPV16-E7-m2 HPV16-E7 (Other) Luciferase Jung et al Mol Cancer. 2014 Apr 8;13(1):80. doi: 10.1186/1476-4598-13-80. Luciferase reporter assay for HPV16 E7 with point mutation on miR-375 binding site pMiR-Target
53699 pMiR-HPV16-E1(partial) HPV16-E1(partial) (Other) Luciferase Jung et al Mol Cancer. 2014 Apr 8;13(1):80. doi: 10.1186/1476-4598-13-80. Luciferase reporter assay for microRNA binding on HPV16 E1 pMiR-Target
53700 pMiR-HPV16-E1-m3 HPV16-E1(partial) (Other) Luciferase Jung et al Mol Cancer. 2014 Apr 8;13(1):80. doi: 10.1186/1476-4598-13-80. Luciferase reporter assay for HPV16 E7 with point mutation on miR-375 binding site pMiR-Target
53701 pMiR-HPV16-E1-m4 HPV16-E1(partial) (Other) Luciferase Jung et al Mol Cancer. 2014 Apr 8;13(1):80. doi: 10.1186/1476-4598-13-80. Luciferase reporter assay for HPV16 E7 with point mutation on miR-375 binding site pMiR-Target
53702 pMiR-HPV16-E1-m5 HPV16-E1(partial) (Other) Luciferase Jung et al Mol Cancer. 2014 Apr 8;13(1):80. doi: 10.1186/1476-4598-13-80. Luciferase reporter assay for HPV16 E7 with point mutation on miR-375 binding site pMiR-Target
53703 pMiR-HPV16-E7/E1 HPV16-E7/E1 (Other) Luciferase Jung et al Mol Cancer. 2014 Apr 8;13(1):80. doi: 10.1186/1476-4598-13-80. Luciferase reporter assay for microRNA binding on HPV16 E7/E1 pMiR-Target
53704 pMiR-HPV16-E7/E1-miR-375-m1-5 HPV16-E7/E1 (Other) Luciferase Jung et al Mol Cancer. 2014 Apr 8;13(1):80. doi: 10.1186/1476-4598-13-80. Luciferase reporter assay for HPV16 E7/E1 with point mutation on miR-375 binding site pMiR-Target
53705 pMiR-CIP2A(partial) CIP2A (Homo sapiens) Luciferase Jung et al Mol Biol Cell. 2013 Jun;24(11):1638-48, S1-7. doi: 10.1091/mbc.E12-12-0891. Epub 2013 Apr 3. Luciferase reporter assay for microRNA binding on CIP2A coding sequence pMiR-Target
53706 pMiR-CIP2A-miR-375-mutA CIP2A (Homo sapiens) Luciferase Jung et al Mol Biol Cell. 2013 Jun;24(11):1638-48, S1-7. doi: 10.1091/mbc.E12-12-0891. Epub 2013 Apr 3. Luciferase reporter assay for CIP2A with point mutation on miR-375 binding site pMiR-Target
53707 pMiR-CIP2A-miR-375-mutB CIP2A (Homo sapiens) Luciferase Jung et al Mol Biol Cell. 2013 Jun;24(11):1638-48, S1-7. doi: 10.1091/mbc.E12-12-0891. Epub 2013 Apr 3. Luciferase reporter assay for CIP2A with point mutation on miR-375 binding site pMiR-Target
53708 pMiR-CIP2A-miR-375-mutC CIP2A (Homo sapiens) Luciferase Jung et al Mol Biol Cell. 2013 Jun;24(11):1638-48, S1-7. doi: 10.1091/mbc.E12-12-0891. Epub 2013 Apr 3. Luciferase reporter assay for CIP2A with point mutation on miR-375 binding site pMiR-Target
53709 pMiR-CIP2A-miR-375-mutD CIP2A (Homo sapiens) Luciferase Jung et al Mol Biol Cell. 2013 Jun;24(11):1638-48, S1-7. doi: 10.1091/mbc.E12-12-0891. Epub 2013 Apr 3. Luciferase reporter assay for CIP2A with point mutation on miR-375 binding site pMiR-Target
53710 pMiR-CIP2A-miR-375-mutE CIP2A (Homo sapiens) Luciferase Jung et al Mol Biol Cell. 2013 Jun;24(11):1638-48, S1-7. doi: 10.1091/mbc.E12-12-0891. Epub 2013 Apr 3. Luciferase reporter assay for CIP2A with point mutation on miR-375 binding site pMiR-Target
53711 pMiR-CIP2A-miR-375-mutA&B CIP2A (Homo sapiens) Luciferase Jung et al Mol Biol Cell. 2013 Jun;24(11):1638-48, S1-7. doi: 10.1091/mbc.E12-12-0891. Epub 2013 Apr 3. Luciferase reporter assay for CIP2A with point mutation on miR-375 binding sites pMiR-Target
53712 pMiR-CIP2A-miR-375-mutC&D CIP2A (Homo sapiens) Luciferase Jung et al Mol Biol Cell. 2013 Jun;24(11):1638-48, S1-7. doi: 10.1091/mbc.E12-12-0891. Epub 2013 Apr 3. Luciferase reporter assay for CIP2A with point mutation on miR-375 binding sites pMiR-Target
53713 pMiR-CIP2A-stop-mut CIP2A (Homo sapiens) Luciferase Jung et al Mol Biol Cell. 2013 Jun;24(11):1638-48, S1-7. doi: 10.1091/mbc.E12-12-0891. Epub 2013 Apr 3. Luciferase reporter assay for CIP2A with mutated firefly stop codon pMiR-Target
53714 pMiR-CIP2A-stop-mut-miR-375-mutA-E CIP2A (Homo sapiens) Luciferase Jung et al Mol Biol Cell. 2013 Jun;24(11):1638-48, S1-7. doi: 10.1091/mbc.E12-12-0891. Epub 2013 Apr 3. Luciferase reporter assay for CIP2A with mutated firefly stop codon that also has five miR-375 binding site mutations pMiR-Target
53754 pMiR-CIP2A-miR-375-mutA-E CIP2A (Homo sapiens) Luciferase Jung et al Mol Biol Cell. 2013 Jun;24(11):1638-48, S1-7. doi: 10.1091/mbc.E12-12-0891. Epub 2013 Apr 3. Luciferase reporter assay for CIP2A with point mutation on miR-375 binding sites pMiR-Target