Ezra Burstein Lab Plasmids


The Ezra Burstein Lab has deposited plasmids at Addgene for distribution to the research community. Addgene is a non-profit plasmid repository dedicated to improving life science research.

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Title Authors Publication
A bimolecular affinity purification method under denaturing conditions for rapid isolation of a ubiquitinated protein for mass spectrometry analysis. Maine GN, Li H, Zaidi IW, Basrur V, Elenitoba-Johnson KS, Burstein E Nat Protoc. 2010 Aug;5(8):1447-59. Epub 2010 Jul 22.
pEBB-SBP-HA (unpublished)
ID Plasmid Gene/Insert Vector Type Publication Hidden Extra Search Info  
36098 pEBB-BT-Ubiquitin Ubiquitin (Homo sapiens) Mammalian Expression Maine et al Nat Protoc. 2010 Aug;5(8):1447-59. Epub 2010 Jul 22. pEBB-TB RPS27A CEP80, S27A, UBA80, UBC, UBCEP1, UBCEP80
36099 pEBB-TB Mammalian Expression Maine et al Nat Protoc. 2010 Aug;5(8):1447-59. Epub 2010 Jul 22. pEBB
  • Tag / Fusion Protein
    • BT-tag (C terminal on insert)
  • 36100 pEBB-SBP-HA Mammalian Expression pEBB-SBP-HA (unpublished) pEBB
  • Tags / Fusion Proteins
    • SBP (N terminal on backbone)
    • HA (C terminal on backbone)
  • 36295 pEBB-BT Mammalian Expression Maine et al Nat Protoc. 2010 Aug;5(8):1447-59. Epub 2010 Jul 22. pEBB
  • Tag / Fusion Protein
    • BT-tag (peptide sequence from P.freudenreichii transcarboxylase, that is biotinylated in vivo+TEV-site (N terminal on backbone)