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Ezra Burstein Lab Plasmids


The Ezra Burstein Lab has deposited plasmids at Addgene for distribution to the research community. Addgene is a non-profit plasmid repository dedicated to improving life science research.

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Title Authors Publication
A bimolecular affinity purification method under denaturing conditions for rapid isolation of a ubiquitinated protein for mass spectrometry analysis. Maine GN, Li H, Zaidi IW, Basrur V, Elenitoba-Johnson KS, Burstein E Nat Protoc. 2010 Aug;5(8):1447-59. Epub 2010 Jul 22.
pEBB-SBP-HA (unpublished)
GCN5 is a required cofactor for a ubiquitin ligase that targets NF-kappaB/RelA. Mao X, Gluck N, Li D, Maine GN, Li H, Zaidi IW, Repaka A, Mayo MW, Burstein E Genes Dev. 2009 Apr 1;23(7):849-61. doi: 10.1101/gad.1748409.
COMMD proteins, a novel family of structural and functional homologs of MURR1. Burstein E, Hoberg JE, Wilkinson AS, Rumble JM, Csomos RA, Komarck CM, Maine GN, Wilkinson JC, Mayo MW, Duckett CS J Biol Chem. 2005 Jun 10;280(23):22222-32. Epub 2005 Mar 30.
CCDC22 deficiency in humans blunts activation of proinflammatory NF-kappaB signaling. Starokadomskyy P, Gluck N, Li H, Chen B, Wallis M, Maine GN, Mao X, Zaidi IW, Hein MY, McDonald FJ, Lenzner S, Zecha A, Ropers HH, Kuss AW, McGaughran J, Gecz J, Burstein E J Clin Invest. 2013 May;123(5):2244-56. doi: 10.1172/JCI66466. Epub 2013 Apr 8.
ID Plasmid Gene/Insert Vector Type Publication Hidden Extra Search Info  
36098pEBB-BT-UbiquitinUbiquitin (Homo sapiens)Mammalian ExpressionA bimolecular affinity purification method under denaturing conditions for rapid isolation of a ubiquitinated protein for mass spectrometry analysis. Nat Protoc. 2010 Aug;5(8):1447-59. Epub 2010 Jul 22. pEBB-TB RPS27A CEP80, HEL112, S27A, UBA80, UBC, UBCEP1, UBCEP80 Add to Cart
36099pEBB-TBMammalian ExpressionA bimolecular affinity purification method under denaturing conditions for rapid isolation of a ubiquitinated protein for mass spectrometry analysis. Nat Protoc. 2010 Aug;5(8):1447-59. Epub 2010 Jul 22. pEBB
  • Tag / Fusion Protein
    • BT-tag (C terminal on insert)
  • Add to Cart
    36100pEBB-SBP-HAMammalian Expression pEBB-SBP-HA (unpublished) pEBB
  • Tags / Fusion Proteins
    • SBP (N terminal on backbone)
    • HA (C terminal on backbone)
  • Add to Cart
    36295pEBB-BTMammalian ExpressionA bimolecular affinity purification method under denaturing conditions for rapid isolation of a ubiquitinated protein for mass spectrometry analysis. Nat Protoc. 2010 Aug;5(8):1447-59. Epub 2010 Jul 22. pEBB
  • Tag / Fusion Protein
    • BT-tag (peptide sequence from P.freudenreichii transcarboxylase, that is biotinylated in vivo+TEV-site (N terminal on backbone)
  • Add to Cart
    74784pEBB Flag GCN5GCN5 (Homo sapiens)Mammalian ExpressionGCN5 is a required cofactor for a ubiquitin ligase that targets NF-kappaB/RelA. Genes Dev. 2009 Apr 1;23(7):849-61. doi: 10.1101/gad.1748409. express of GCN5 in mammalian cells pEBB KAT2A GCN5, GCN5L2, PCAF-b, hGCN5 Add to Cart
    74892pEBB HA RelARelA (Homo sapiens)Mammalian ExpressionCOMMD proteins, a novel family of structural and functional homologs of MURR1. J Biol Chem. 2005 Jun 10;280(23):22222-32. Epub 2005 Mar 30. Express RelA in mammalian cells pEBB RELA NFKB3, p65 Add to Cart
    74893pEBB COMMD1 FlagCOMMD1 (Homo sapiens)Mammalian ExpressionCOMMD proteins, a novel family of structural and functional homologs of MURR1. J Biol Chem. 2005 Jun 10;280(23):22222-32. Epub 2005 Mar 30. Express COMMD1 in mammalian cells pEBB COMMD1 C2orf5, MURR1 Add to Cart
    74894pEBB COMMD2 FlagCOMMD2 (Homo sapiens)Mammalian ExpressionCOMMD proteins, a novel family of structural and functional homologs of MURR1. J Biol Chem. 2005 Jun 10;280(23):22222-32. Epub 2005 Mar 30. Express COMMD2 in mammalian cells pEBB COMMD2 HSPC042 Add to Cart
    74895pEBB COMMD3 FlagCOMMD3 (Homo sapiens)Mammalian ExpressionCOMMD proteins, a novel family of structural and functional homologs of MURR1. J Biol Chem. 2005 Jun 10;280(23):22222-32. Epub 2005 Mar 30. Express COMMD3 in mammalian cells pEBB COMMD3 BUP, C10orf8 Add to Cart
    74896pEBB COMMD4 FlagCOMMD4 (Homo sapiens)Mammalian ExpressionCOMMD proteins, a novel family of structural and functional homologs of MURR1. J Biol Chem. 2005 Jun 10;280(23):22222-32. Epub 2005 Mar 30. Express COMMD4 in mammalian cells pEBB COMMD4 Add to Cart
    74897pEBB COMMD5 FlagCOMMD5 (Homo sapiens)Mammalian ExpressionCOMMD proteins, a novel family of structural and functional homologs of MURR1. J Biol Chem. 2005 Jun 10;280(23):22222-32. Epub 2005 Mar 30. Express COMMD5 in mammalian cells pEBB COMMD5 HCARG, HT002 Add to Cart
    74898pEBB COMMD6 FlagCOMMD6 (Homo sapiens)Mammalian ExpressionCOMMD proteins, a novel family of structural and functional homologs of MURR1. J Biol Chem. 2005 Jun 10;280(23):22222-32. Epub 2005 Mar 30. Express COMMD6 in mammalian cells pEBB COMMD6 Acrg Add to Cart
    74899pEBB COMMD7 FlagCOMMD7 (Homo sapiens)Mammalian ExpressionCOMMD proteins, a novel family of structural and functional homologs of MURR1. J Biol Chem. 2005 Jun 10;280(23):22222-32. Epub 2005 Mar 30. Express COMMD7 in mammalian cells pEBB COMMD7 C20orf92, dJ1085F17.3 Add to Cart
    74900pEBB COMMD8 FlagCOMMD8 (Homo sapiens)Mammalian ExpressionCOMMD proteins, a novel family of structural and functional homologs of MURR1. J Biol Chem. 2005 Jun 10;280(23):22222-32. Epub 2005 Mar 30. Express COMMD8 in mammalian cells pEBB COMMD8 Add to Cart
    74901pEBB COMMD9 FlagCOMMD9 (Homo sapiens)Mammalian ExpressionCOMMD proteins, a novel family of structural and functional homologs of MURR1. J Biol Chem. 2005 Jun 10;280(23):22222-32. Epub 2005 Mar 30. Express COMMD9 in mammalian cells pEBB COMMD9 HSPC166 Add to Cart
    74902pEBB COMMD10 FlagCOMMD10 (Homo sapiens)Mammalian ExpressionCOMMD proteins, a novel family of structural and functional homologs of MURR1. J Biol Chem. 2005 Jun 10;280(23):22222-32. Epub 2005 Mar 30. Express COMMD10 in mammalian cells pEBB COMMD10 PTD002 Add to Cart
    74903pEBB HA CCDC22CCDC22 (Homo sapiens)Mammalian ExpressionCCDC22 deficiency in humans blunts activation of proinflammatory NF-kappaB signaling. J Clin Invest. 2013 May;123(5):2244-56. doi: 10.1172/JCI66466. Epub 2013 Apr 8. Express CCDC22 in mammalian cells pEBB CCDC22 CXorf37, JM1, RTSC2 Add to Cart