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Gabor Balazsi Lab Plasmids


The Gabor Balazsi Lab has deposited plasmids at Addgene for distribution to the research community. Addgene is a non-profit plasmid repository dedicated to improving life science research.

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Title Authors Publication
Transferring a synthetic gene circuit from yeast to mammalian cells. Nevozhay D, Zal T, Balazsi G Nat Commun. 2013 Feb 5;4:1451. doi: 10.1038/ncomms2471.
Mapping the environmental fitness landscape of a synthetic gene circuit. Nevozhay D, Adams RM, Van Itallie E, Bennett MR, Balazsi G PLoS Comput Biol. 2012;8(4):e1002480. doi: 10.1371/journal.pcbi.1002480. Epub 2012 Apr 12.
Negative autoregulation linearizes the dose-response and suppresses the heterogeneity of gene expression. Nevozhay D, Adams RM, Murphy KF, Josic K, Balazsi G Proc Natl Acad Sci U S A. 2009 Mar 31;106(13):5123-8. doi: 10.1073/pnas.0809901106. Epub 2009 Mar 11.
Plasmid Gene/Insert Vector Type Publication Hidden Extra Search Info  
pDN-G1Gh PGal1-D12 promoter (Saccharomyces cerevisiae), yEGFP (Saccharomyces cerevisiae) Yeast Expression, Synthetic Biology ; Expression regulator/reporter Negative autoregulation linearizes the dose-response and suppresses the heterogeneity of gene expression. Nevozhay et al Proc Natl Acad Sci U S A. 2009 Mar 31;106(13):5123-8. doi: 10.1073/pnas.0809901106. Epub 2009 Mar 11. pRS403 GAL1 YBR020W Add to Cart
pDN-G1TGt PGal1-D12 promoter (Saccharomyces cerevisiae), tetR::yEGFP (Saccharomyces cerevisiae) Yeast Expression, Synthetic Biology ; Expression regulator/reporter Negative autoregulation linearizes the dose-response and suppresses the heterogeneity of gene expression. Nevozhay et al Proc Natl Acad Sci U S A. 2009 Mar 31;106(13):5123-8. doi: 10.1073/pnas.0809901106. Epub 2009 Mar 11. pRS404 GAL1 YBR020W Add to Cart
pDN-G1Tt PGal1-D12 promoter (Saccharomyces cerevisiae), tetR (Synthetic) Yeast Expression, Synthetic Biology ; Expression regulator/reporter Negative autoregulation linearizes the dose-response and suppresses the heterogeneity of gene expression. Nevozhay et al Proc Natl Acad Sci U S A. 2009 Mar 31;106(13):5123-8. doi: 10.1073/pnas.0809901106. Epub 2009 Mar 11. pRS404 GAL1 YBR020W Add to Cart
pDN-T1TCbt PGal1-T123 promoter (Saccharomyces cerevisiae), tetR::ymcherry (Saccharomyces cerevisiae) Yeast Expression, Synthetic Biology ; Expression regulator/reporter Negative autoregulation linearizes the dose-response and suppresses the heterogeneity of gene expression. Nevozhay et al Proc Natl Acad Sci U S A. 2009 Mar 31;106(13):5123-8. doi: 10.1073/pnas.0809901106. Epub 2009 Mar 11. pRS404 GAL1 YBR020W Add to Cart
pDN-G1TCbt PGal1-D12 promoter (Saccharomyces cerevisiae), tetR::ymcherry (Saccharomyces cerevisiae) Yeast Expression, Synthetic Biology ; Expression regulator/reporter Negative autoregulation linearizes the dose-response and suppresses the heterogeneity of gene expression. Nevozhay et al Proc Natl Acad Sci U S A. 2009 Mar 31;106(13):5123-8. doi: 10.1073/pnas.0809901106. Epub 2009 Mar 11. pRS404 GAL1 YBR020W Add to Cart
pDN-G1GZmbh PGal1-D12 promoter (Saccharomyces cerevisiae), yEGFP::ZeoR (Saccharomyces cerevisiae) Yeast Expression, Synthetic Biology ; Expression regulator/reporter Negative autoregulation linearizes the dose-response and suppresses the heterogeneity of gene expression. Nevozhay et al Proc Natl Acad Sci U S A. 2009 Mar 31;106(13):5123-8. doi: 10.1073/pnas.0809901106. Epub 2009 Mar 11. pRS403 GAL1 YBR020W Add to Cart
pDN-T1GZmbh PGal1-T123 promoter (Saccharomyces cerevisiae), yEGFP::ZeoR (Saccharomyces cerevisiae) Yeast Expression, Synthetic Biology ; Expression regulator/reporter Negative autoregulation linearizes the dose-response and suppresses the heterogeneity of gene expression. Nevozhay et al Proc Natl Acad Sci U S A. 2009 Mar 31;106(13):5123-8. doi: 10.1073/pnas.0809901106. Epub 2009 Mar 11. pRS403 GAL1 YBR020W Add to Cart
pDN-NG1Tt PGal1 (wt) promoter (Saccharomyces cerevisiae), tetR (Synthetic) Yeast Expression, Synthetic Biology ; Expression regulator/reporter Negative autoregulation linearizes the dose-response and suppresses the heterogeneity of gene expression. Nevozhay et al Proc Natl Acad Sci U S A. 2009 Mar 31;106(13):5123-8. doi: 10.1073/pnas.0809901106. Epub 2009 Mar 11. pRS404 GAL1 YBR020W Add to Cart
pDN-T2dGZmxh PTETREG promoter (Synthetic), yEGFP::ZeoR (Saccharomyces cerevisiae) Yeast Expression, Synthetic Biology ; Expression regulator/reporter Mapping the environmental fitness landscape of a synthetic gene circuit. Nevozhay et al PLoS Comput Biol. 2012;8(4):e1002480. doi: 10.1371/journal.pcbi.1002480. Epub 2012 Apr 12. pRS403 Add to Cart
pDN-T2dMFot PTETREG promoter (Synthetic), rtetR-M2::FFF (Synthetic) Yeast Expression, Synthetic Biology ; Expression regulator/reporter Mapping the environmental fitness landscape of a synthetic gene circuit. Nevozhay et al PLoS Comput Biol. 2012;8(4):e1002480. doi: 10.1371/journal.pcbi.1002480. Epub 2012 Apr 12. pRS404 Add to Cart
pDN-D2irTNG4kwh pCMV-D2i promoter (Synthetic), htetR::NLS::EGFP (Homo sapiens) Mammalian Expression, Synthetic Biology ; Expression regulator/reporter; Expression "Linearizer" system Transferring a synthetic gene circuit from yeast to mammalian cells. Nevozhay et al Nat Commun. 2013 Feb 5;4:1451. doi: 10.1038/ncomms2471. pcDNA4/TO Add to Cart
pDN-D2irG4kwh pCMV-D2i promoter (Synthetic), EGFP (Homo sapiens) Mammalian Expression, Synthetic Biology ; Expression regulator/reporter Transferring a synthetic gene circuit from yeast to mammalian cells. Nevozhay et al Nat Commun. 2013 Feb 5;4:1451. doi: 10.1038/ncomms2471. pcDNA4/TO
  • Tags / Fusion Proteins
    • Rabbit β-globin intron II (N terminal on insert)
    • WPRE (C terminal on insert)
  • Add to Cart
    pDN-D2irTN6kwh pCMV-D2i promoter (Synthetic), htetR::NLS (Homo sapiens) Mammalian Expression, Synthetic Biology ; Expression regulator/reporter; Expression "Linearizer" system Transferring a synthetic gene circuit from yeast to mammalian cells. Nevozhay et al Nat Commun. 2013 Feb 5;4:1451. doi: 10.1038/ncomms2471. pcDNA6/TR
  • Tags / Fusion Proteins
    • Rabbit β-globin intron II (N terminal on insert)
    • WPRE (C terminal on insert)
  • Add to Cart
    pDN-D2irC6kwh pCMV-D2i promoter (Synthetic), mCherry (Homo sapiens) Mammalian Expression, Synthetic Biology ; Expression regulator/reporter Transferring a synthetic gene circuit from yeast to mammalian cells. Nevozhay et al Nat Commun. 2013 Feb 5;4:1451. doi: 10.1038/ncomms2471. pcDNA6/TR
  • Tags / Fusion Proteins
    • Rabbit β-globin intron II (N terminal on insert)
    • WPRE (C terminal on insert)
  • Add to Cart