Geraldine Seydoux Lab Plasmids


The Geraldine Seydoux Lab has deposited plasmids at Addgene for distribution to the research community. Addgene is a non-profit plasmid repository dedicated to improving life science research.

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Title Authors Publication
Microtubules induce self-organization of polarized PAR domains in Caenorhabditis elegans zygotes. Motegi F, Zonies S, Hao Y, Cuenca AA, Griffin E, Seydoux G Nat Cell Biol. 2011 Oct 9;13(11):1361-7. doi: 10.1038/ncb2354.
Regulation of the MEX-5 gradient by a spatially segregated kinase/phosphatase cycle. Griffin EE, Odde DJ, Seydoux G Cell. 2011 Sep 16;146(6):955-68.
The C. elegans homolog of nucleoporin Nup98 is required for the integrity and function of germline P granules. Voronina E, Seydoux G Development. 2010 May;137(9):1441-50. Epub 2010 Mar 24.
3' UTRs Are the Primary Regulators of Gene Expression in the C. elegans Germline. Merritt C, Rasoloson D, Ko D, Seydoux G Curr Biol. 2008 Sep 23. ():.
Processing bodies and germ granules are distinct RNA granules that interact in C. elegans embryos. Gallo CM, Munro E, Rasoloson D, Merritt C, Seydoux G Dev Biol. 2008 Nov 1. 323(1):76-87.
Translational repression restricts expression of the C. elegans Nanos homolog NOS-2 to the embryonic germline. D'Agostino I, Merritt C, Chen PL, Seydoux G, Subramaniam K Dev Biol. 2006 Apr 1. 292(1):244-52.
Fourth generation Seydoux lab vectors (unpublished)
Forth generation Seydoux lab vectors (unpublished)
Seydoux Lab - Third Generation Vector Kit (unpublished)
ID Plasmid Gene/Insert Vector Type Publication Hidden Extra Search Info  
17246 pCG142 pie-1 intron:pie-1 promoter in PDONRP4P1R (Caenorhabditis elegans) Gateway Seydoux Lab - Third Generation Vector Kit (unpublished) PDONRP4P1R
17247 pCG150 unc-119 rescue fragment in pDESTR4R3 (Caenorhabditis elegans) Gateway Seydoux Lab - Third Generation Vector Kit (unpublished) pDESTR4R3
17248 pCM1.35 GFP:H2B from pKS111-His in pDONR201 (Caenorhabditis elegans) Gateway Seydoux Lab - Third Generation Vector Kit (unpublished) pDONR201
17249 pCM1.36 tbb-2 3'UTR in pDONRP2RP3 (Caenorhabditis elegans) Gateway Seydoux Lab - Third Generation Vector Kit (unpublished) pDONRP2RP3
17250 pCM1.53 GFP-no stop in pDONR201 (Caenorhabditis elegans) Gateway Seydoux Lab - Third Generation Vector Kit (unpublished) pDONR201
17251 pCM1.56 L3787 promoter NO ATG in pDONRP4P1R (Caenorhabditis elegans) Gateway Seydoux Lab - Third Generation Vector Kit (unpublished) pDONRP4P1R
17252 pCM1.57 L3788 promoter NO ATG in pDONRP4P1R (Caenorhabditis elegans) Gateway Seydoux Lab - Third Generation Vector Kit (unpublished) pDONRP4P1R
17253 pCM5.37 unc-54 3' from pPD49.78 in pDONRP2RP3 (Caenorhabditis elegans) Gateway Seydoux Lab - Third Generation Vector Kit (unpublished) pDONRP2RP3
17254 pCM5.47 pie-1 3'utr in pDONRP2RP3 (Caenorhabditis elegans) Gateway Seydoux Lab - Third Generation Vector Kit (unpublished) pDONRP2RP3
19366 pDR3.13 him-3 ORF+3' in pDONR201 (Caenorhabditis elegans) Worm Expression Fourth generation Seydoux lab vectors (unpublished) pDONR201 him-3
19367 pDR3.10 msp-142 ORF+3' in pDONR201 (Caenorhabditis elegans) Worm Expression Fourth generation Seydoux lab vectors (unpublished) pDONR201
19368 pDR3.06 daz-1 ORF+3' in pDONR201 (Caenorhabditis elegans) Worm Expression Fourth generation Seydoux lab vectors (unpublished) pDONR201
19369 pDR3.01 spn-4 ORF+3' in pDONR201 (Caenorhabditis elegans) Worm Expression Fourth generation Seydoux lab vectors (unpublished) pDONR201
19370 pCM5.66 pos-1 3'utr in pDONRP2RP3 (Caenorhabditis elegans) Worm Expression Fourth generation Seydoux lab vectors (unpublished) pDONRP2RP3
19371 pCM5.65 mes-3 3'utr in pDONRP2RP3 (Caenorhabditis elegans) Worm Expression Fourth generation Seydoux lab vectors (unpublished) pDONRP2RP3
19372 pCM5.64 puf-5 3'utr in pDONRP2RP3 (Caenorhabditis elegans) Worm Expression Fourth generation Seydoux lab vectors (unpublished) pDONRP2RP3
19373 pCM5.63 spe-41 3'utr in pDONRP2RP3 (Caenorhabditis elegans) Worm Expression Fourth generation Seydoux lab vectors (unpublished) pDONRP2RP3
19374 pCM5.60 fog-1 3'utr in pDONRP2RP3 (Caenorhabditis elegans) Worm Expression Fourth generation Seydoux lab vectors (unpublished) pDONRP2RP3
19375 pCM5.58 spe-38 3'utr in pDONRP2RP3 (Caenorhabditis elegans) Worm Expression Fourth generation Seydoux lab vectors (unpublished) pDONRP2RP3
19378 pCM5.57 spe-11 3'utr in pDONRP2RP3 (Caenorhabditis elegans) Worm Expression Fourth generation Seydoux lab vectors (unpublished) pDONRP2RP3
19379 pCM5.56 msp-142 3'utr in pDONRP2RP3 (Caenorhabditis elegans) Worm Expression Fourth generation Seydoux lab vectors (unpublished) pDONRP2RP3
19381 pCM5.52 him-3 3'utr in pDONRP2RP3 (Caenorhabditis elegans) Worm Expression Fourth generation Seydoux lab vectors (unpublished) pDONRP2RP3
19382 pCM5.51 rme-2 3'utr in pDONRP2RP3 (Caenorhabditis elegans) Worm Expression Fourth generation Seydoux lab vectors (unpublished) pDONRP2RP3
19383 pCM5.50 spn-4 3'utr in pDONRP2RP3 (Caenorhabditis elegans) Worm Expression Fourth generation Seydoux lab vectors (unpublished) pDONRP2RP3
19385 pCM5.48 mex-5 3'utr in pDONRP2RP3 (Caenorhabditis elegans) Worm Expression Fourth generation Seydoux lab vectors (unpublished) pDONRP2RP3
19386 pCM5.46 pgl-3 3'utr in pDONRP2RP3 (Caenorhabditis elegans) Worm Expression Fourth generation Seydoux lab vectors (unpublished) pDONRP2RP3
19387 pCM5.45 mex-3 3'utr in pDONRP2RP3 (Caenorhabditis elegans) Worm Expression Fourth generation Seydoux lab vectors (unpublished) pDONRP2RP3
19389 pCM5.44 cep-1 3'utr in pDONRP2RP3 (Caenorhabditis elegans) Worm Expression Fourth generation Seydoux lab vectors (unpublished) pDONRP2RP3
19390 pCM5.42 fog-2 3'utr in pDONRP2RP3 (Caenorhabditis elegans) Worm Expression Fourth generation Seydoux lab vectors (unpublished) pDONRP2RP3
19391 pCM5.43 daz-1 3'utr in pDONRP2RP3 (Caenorhabditis elegans) Worm Expression Fourth generation Seydoux lab vectors (unpublished) pDONRP2RP3
19392 pCM5.41 lip-1 3'utr in pDONRP2RP3 (Caenorhabditis elegans) Worm Expression Fourth generation Seydoux lab vectors (unpublished) pDONRP2RP3
19393 pCM5.40 glp-1 3'utr in pDONRP2RP3 (Caenorhabditis elegans) Worm Expression Fourth generation Seydoux lab vectors (unpublished) pDONRP2RP3
19394 pCM5.36 gld-1 3'utr in pDONRP2RP3 (Caenorhabditis elegans) Worm Expression Fourth generation Seydoux lab vectors (unpublished) pDONRP2RP3 gld-1 CELE_T23G11.3
19395 pCM5.35 nos-3 3'utr in pDONRP2RP3 (Caenorhabditis elegans) Worm Expression Fourth generation Seydoux lab vectors (unpublished) pDONRP2RP3
19396 pCM5.33 fbf-1 3'utr in pDONRP2RP3 (Caenorhabditis elegans) Worm Expression Fourth generation Seydoux lab vectors (unpublished) pDONRP2RP3
19397 pCM5.32 mes-2 3'utr in pDONRP2RP3 (Caenorhabditis elegans) Worm Expression Fourth generation Seydoux lab vectors (unpublished) pDONRP2RP3
19398 pCM5.31 par-5 3'utr in pDONRP2RP3 (Caenorhabditis elegans) Worm Expression Fourth generation Seydoux lab vectors (unpublished) pDONRP2RP3
19399 pCM5.30 pgl-1 3'utr in pDONRP2RP3 (Caenorhabditis elegans) Worm Expression Fourth generation Seydoux lab vectors (unpublished) pDONRP2RP3
19401 pCM3.19 tbb-2 ORF+3' in pDONR201 (Caenorhabditis elegans) Worm Expression Fourth generation Seydoux lab vectors (unpublished) pDONR201 tbb-2
19402 pCM3.29 lip-1 ORF+3' in pDONR201 (Caenorhabditis elegans) Worm Expression Fourth generation Seydoux lab vectors (unpublished) pDONR201 lip-1
19403 pCM3.32 nos-3 ORF+3' in pDONR201 (Caenorhabditis elegans) Worm Expression Fourth generation Seydoux lab vectors (unpublished) pDONR201 nos-3
19404 pCM3.35 par-5 ORF+3' in pDONR201 (Caenorhabditis elegans) Worm Expression Fourth generation Seydoux lab vectors (unpublished) pDONR201 par-5 CELE_M117.2
19405 pCM3.08 pie-1 ORF+3' in pDONR201 (Caenorhabditis elegans) Worm Expression Fourth generation Seydoux lab vectors (unpublished) pDONR201 pie-1 Y49E10.14
19406 pCM3.09 fbf-2 ORF+3' in pDONR201 (Caenorhabditis elegans) Worm Expression Fourth generation Seydoux lab vectors (unpublished) pDONR201 fbf-2 F21H12.5
19592 pCM1.52 lip-1 prom in PDONRP4P1R (Caenorhabditis elegans) Worm Expression Forth generation Seydoux lab vectors (unpublished) PDONRP4P1R
19593 pCM1.59 gld-1 prom in PDONRP4P1R (Caenorhabditis elegans) Worm Expression Forth generation Seydoux lab vectors (unpublished) PDONRP4P1R
19594 pCM1.60 daz-1 prom in PDONRP4P1R (Caenorhabditis elegans) Worm Expression Forth generation Seydoux lab vectors (unpublished) PDONRP4P1R
19595 pCM1.107 fbf-1 prom in PDONRP4P1R (Caenorhabditis elegans) Worm Expression Forth generation Seydoux lab vectors (unpublished) PDONRP4P1R
19597 pCM1.109 pgl-3 prom in PDONRP4P1R (Caenorhabditis elegans) Worm Expression Forth generation Seydoux lab vectors (unpublished) PDONRP4P1R
19598 pCM1.111 mex-5 prom in PDONRP4P1R (Caenorhabditis elegans) Worm Expression Forth generation Seydoux lab vectors (unpublished) PDONRP4P1R
19599 pCM1.112 fbf-2 prom in PDONRP4P1R (Caenorhabditis elegans) Worm Expression Forth generation Seydoux lab vectors (unpublished) PDONRP4P1R
19600 pCM1.120 spn-4 prom in PDONRP4P1R (Caenorhabditis elegans) Worm Expression Forth generation Seydoux lab vectors (unpublished) PDONRP4P1R
19601 pCM3.05 mex-5 ORF+3' in pDONR201 (Caenorhabditis elegans) Worm Expression Forth generation Seydoux lab vectors (unpublished) pDONR201 mex-5 CELE_W02A2.7
19602 pCM3.06 fbf-1 ORF+3' in pDONR201 (Caenorhabditis elegans) Worm Expression Forth generation Seydoux lab vectors (unpublished) pDONR201 fbf-1 CELE_H12I13.4
19603 pCM3.07 pal-1 ORF+3' in pDONR201 (Caenorhabditis elegans) Worm Expression Forth generation Seydoux lab vectors (unpublished) pDONR201 pal-1 CELE_C38D4.6
19604 pCM3.10 pgl-3 ORF+3' in pDONR201 (Caenorhabditis elegans) Worm Expression Forth generation Seydoux lab vectors (unpublished) pDONR201 pgl-3 C18G1.4
19605 pCM3.11 pgl-1 ORF+3' in pDONR201 (Caenorhabditis elegans) Worm Expression Forth generation Seydoux lab vectors (unpublished) pDONR201 pgl-1 ZK381.4
19606 pCM3.12 spe-11 ORF+3' in pDONR201 (Caenorhabditis elegans) Worm Expression Forth generation Seydoux lab vectors (unpublished) pDONR201 spe-11
19607 pCM3.17 spe-38 ORF+3' in pDONR201 (Caenorhabditis elegans) Worm Expression Forth generation Seydoux lab vectors (unpublished) pDONR201 spe-38
21376 pCG144 mCherry in pDONR201 (Caenorhabditis elegans) Gateway Merritt et al Curr Biol. 2008 Sep 23. ():. pDONR201
21377 pCM1.26 L3787 promoter::GFP in pDONRP4P1R (Caenorhabditis elegans) Gateway Merritt et al Curr Biol. 2008 Sep 23. ():. pDONRP4P1R
21378 pCM1.27 L3788 promoter::GFP in pDONRP4P1R (Caenorhabditis elegans) Gateway Merritt et al Curr Biol. 2008 Sep 23. ():. pDONRP4P1R
21379 pCM1.41 spe-11 promoter in pDONRP4P1R (Caenorhabditis elegans) Gateway Merritt et al Curr Biol. 2008 Sep 23. ():. pDONRP4P1R
21380 pCM1.58 pie-1 promoter (short) in pDONRP4P1R (Caenorhabditis elegans) Gateway Merritt et al Curr Biol. 2008 Sep 23. ():. pDONRP4P1R
21381 pCM1.95 lip-1 M1M2 3'utr in pDONRP2RP3 (Caenorhabditis elegans) Gateway Merritt et al Curr Biol. 2008 Sep 23. ():. pDONRP2RP3
21382 pCM1.108 him-3 prom in PDONRP4P1R (Caenorhabditis elegans) Gateway Merritt et al Curr Biol. 2008 Sep 23. ():. PDONRP4P1R
21383 pCM1.122 msp-56 promoter in pDONRP4P1R (Caenorhabditis elegans) Gateway Merritt et al Curr Biol. 2008 Sep 23. ():. pDONRP4P1R
21384 pCM1.127 pie-1 promoter (short, no ATG) in pDONRP4P1R (Caenorhabditis elegans) Gateway Merritt et al Curr Biol. 2008 Sep 23. ():. pDONRP4P1R
21385 pCM1.128 spe-11 promoter (no ATG) in pDONRP4P1R (Caenorhabditis elegans) Gateway Merritt et al Curr Biol. 2008 Sep 23. ():. pDONRP4P1R
21386 pCM1.151 mCherry::histone H2B in pDONR201 (Caenorhabditis elegans) Gateway Merritt et al Curr Biol. 2008 Sep 23. ():. pDONR201
21387 pCM1.153 gld-1 M1M2 3'utr in pDONRP2RP3 (Caenorhabditis elegans) Gateway Merritt et al Curr Biol. 2008 Sep 23. ():. pDONRP2RP3
21388 pCM1.157 gld-1/cye-1 operon in pDONRP2RP3 (Caenorhabditis elegans) Gateway Merritt et al Curr Biol. 2008 Sep 23. ():. pDONRP2RP3
21389 pCM3.23 gld-1 ORF+3' in pDONR201 (Caenorhabditis elegans) Gateway Merritt et al Curr Biol. 2008 Sep 23. ():. pDONR201 gld-1 CELE_T23G11.3
21390 pCM5.34 fbf-2 3'utr in pDONRP2RP3 (Caenorhabditis elegans) Gateway Merritt et al Curr Biol. 2008 Sep 23. ():. pDONRP2RP3
21391 pCM5.49 pal-1 3'utr in pDONRP2RP3 (Caenorhabditis elegans) Gateway Merritt et al Curr Biol. 2008 Sep 23. ():. pDONRP2RP3
21392 pCM5.54 cye-1 3'utr in pDONRP2RP3 (Caenorhabditis elegans) Gateway Merritt et al Curr Biol. 2008 Sep 23. ():. pDONRP2RP3
22720 pID3.01B unc-119 rescuing fragment / pie-1promoter-GFP- gateway destination cassette B -pie-1 3’ (Caenorhabditis elegans) Bacterial Expression D'Agostino et al Dev Biol. 2006 Apr 1. 292(1):244-52. n/a
26938 pCG26 dcap-1 (Caenorhabditis elegans) Worm Expression Gallo et al Dev Biol. 2008 Nov 1. 323(1):76-87. unknown dcap-1
26939 pCG31 lsm-1 (Caenorhabditis elegans) Worm Expression Gallo et al Dev Biol. 2008 Nov 1. 323(1):76-87. unknown lsm-1
26940 pCG34 cgh-1 (Caenorhabditis elegans) Worm Expression Gallo et al Dev Biol. 2008 Nov 1. 323(1):76-87. unknown cgh-1
26944 pCG111 lsm-3 (Caenorhabditis elegans) Worm Expression Gallo et al Dev Biol. 2008 Nov 1. 323(1):76-87. unknown lsm-3
26946 pJH4.52 histone H2B (Caenorhabditis elegans) Worm Expression Gallo et al Dev Biol. 2008 Nov 1. 323(1):76-87. pie-1 his-58
26948 pJH4.67 gamma-tubulin (Caenorhabditis elegans) Worm Expression Gallo et al Dev Biol. 2008 Nov 1. 323(1):76-87. pie-1 tbg-1
26951 pJH4.66 beta-tubulin (Caenorhabditis elegans) Worm Expression Gallo et al Dev Biol. 2008 Nov 1. 323(1):76-87. pie-1 tbb-1
26952 pKR2.40 pie-1promoter-GFP- gateway destination cassette B -pie-1 3’ Worm Expression Gallo et al Dev Biol. 2008 Nov 1. 323(1):76-87. pie-1
26955 pID2.02 unc-119 rescuing fragment/pie-1promoter-gateway destination cassette B- pie-1 3’ (Caenorhabditis elegans) Worm Expression Gallo et al Dev Biol. 2008 Nov 1. 323(1):76-87. pie-1
37487 pEV1.02 GLH-1::nos-2 3’UTR (Caenorhabditis elegans) Worm Expression Voronina et al Development. 2010 May;137(9):1441-50. Epub 2010 Mar 24. pEV1
37495 pEV9.03 npp-16 (Caenorhabditis elegans) Worm Expression, RNAi Voronina et al Development. 2010 May;137(9):1441-50. Epub 2010 Mar 24. pL4440gw npp-16 Y56A3A.17
37498 pEV1.05 FBF-2::fbf-2 3’UTR (Caenorhabditis elegans) Worm Expression Voronina et al Development. 2010 May;137(9):1441-50. Epub 2010 Mar 24. pEV1
40074 pEG196 Mex-5 (Caenorhabditis elegans) Bacterial Expression Griffin et al Cell. 2011 Sep 16;146(6):955-68. pMal c2X (GW)
  • Tag / Fusion Protein
    • MBP (N terminal on backbone)
  • mex-5 CELE_W02A2.7
    40075 pEG268 Mex-5 (S458A) (Caenorhabditis elegans) Bacterial Expression Griffin et al Cell. 2011 Sep 16;146(6):955-68. pMal c2X (GW)
  • Tag / Fusion Protein
    • MBP (N terminal on backbone)
  • mex-5 CELE_W02A2.7
    40076 pFM032 Worm Expression Motegi et al Nat Cell Biol. 2011 Oct 9;13(11):1361-7. doi: 10.1038/ncb2354. pIC26
  • Tags / Fusion Proteins
    • Dendra2 PAFP (N terminal on backbone)
    • TEV protease cleavage site (N terminal on backbone)
    • S-peptide (N terminal on backbone)
  • 40077 pEG345 Mex-5 (Caenorhabditis elegans) Worm Expression Griffin et al Cell. 2011 Sep 16;146(6):955-68. pIC26
  • Tag / Fusion Protein
    • S-peptide (N terminal on backbone)
  • mex-5 CELE_W02A2.7
    40086 pEG408 Mex-5 (aa 345 - 468) (Caenorhabditis elegans) Worm Expression Griffin et al Cell. 2011 Sep 16;146(6):955-68. pIC26
  • Tags / Fusion Proteins
    • Dendra2 PFAF (N terminal on backbone)
    • TEV protease cleavage site (N terminal on backbone)
    • S-peptide (N terminal on backbone)
  • mex-5 CELE_W02A2.7
    40087 pEG412 Worm Expression Griffin et al Cell. 2011 Sep 16;146(6):955-68. pIC26
  • Tags / Fusion Proteins
    • Dendra2 PAFP (N terminal on backbone)
    • TEV protease cleavage site (N terminal on backbone)
    • S-peptide (N terminal on backbone)
  • 40088 pEG459 Mex-5 (S404A) (Caenorhabditis elegans) Bacterial Expression Griffin et al Cell. 2011 Sep 16;146(6):955-68. pMal c2X (GW)
  • Tag / Fusion Protein
    • MBP (N terminal on backbone)
  • mex-5 CELE_W02A2.7
    40089 pEG495 Mex-5 (S404A, S458A) (Caenorhabditis elegans) Bacterial Expression Griffin et al Cell. 2011 Sep 16;146(6):955-68. pMal c2X (GW)
  • Tag / Fusion Protein
    • MBP (N terminal on backbone)
  • mex-5 CELE_W02A2.7
    40116 pEG545 Gateway Donor vector Griffin et al Cell. 2011 Sep 16;146(6):955-68. pDONR201
  • Tags / Fusion Proteins
    • Dendra2 PAFP (sequence optimized for worm expression; three synthetic introns inserted) (N terminal on backbone)
    • TEV protease cleavage site (N terminal on backbone)
    • S-peptide (N terminal on backbone)
  • 40119 pEG593 Mex-5 (ATG through 648 bp of 3'UTR) R274E K318E (RNAi res) (Caenorhabditis elegans) Worm Expression ; Gateway Destination vector Griffin et al Cell. 2011 Sep 16;146(6):955-68. pCG150
  • Tags / Fusion Proteins
    • Dendra2 PAFP (sequence optimized for worm expression; three synthetic introns inserted) (N terminal on backbone)
    • TEV protease cleavage site (N terminal on backbone)
    • S-peptide (N terminal on backbone)
  • mex-5 CELE_W02A2.7
    40120 pEG628 Mex-5 (aa1-244) (RNAi resistant) (Caenorhabditis elegans) Worm Expression ; Gateway Destination vector Griffin et al Cell. 2011 Sep 16;146(6):955-68. pCG150
  • Tags / Fusion Proteins
    • Dendra2 PAFP (sequence optimized for worm expression; three synthetic introns inserted) (N terminal on backbone)
    • TEV protease cleavage site (N terminal on backbone)
    • S-peptide (N terminal on backbone)
  • mex-5 CELE_W02A2.7
    40121 pEG634 Mex-5 (aa244-468) (Caenorhabditis elegans) Worm Expression ; Gateway Destination vector Griffin et al Cell. 2011 Sep 16;146(6):955-68. pCG150
  • Tags / Fusion Proteins
    • Dendra2 PAFP (sequence optimized for worm expression; three synthetic introns inserted) (N terminal on backbone)
    • TEV protease cleavage site (N terminal on backbone)
    • S-peptide (N terminal on backbone)
  • mex-5 CELE_W02A2.7
    40122 pEG795 Mex-5 (ATG through 648 bp of 3'UTR) (RNAi resistant exon 2) (Caenorhabditis elegans) Gateway Donor vector Griffin et al Cell. 2011 Sep 16;146(6):955-68. pDONR P2RP3 mex-5 CELE_W02A2.7
    40137 pFM034 par-2 RNAi resistant (wild-type) Worm Expression ; Gateway Destination vector Motegi et al Nat Cell Biol. 2011 Oct 9;13(11):1361-7. doi: 10.1038/ncb2354. pID3.01 par-2 CELE_F58B6.3
    40139 pFM036 par-2 RNAi resistant (K162A) (Caenorhabditis elegans) Worm Expression ; Gateway Destination vector Motegi et al Nat Cell Biol. 2011 Oct 9;13(11):1361-7. doi: 10.1038/ncb2354. pID3.01
  • Tag / Fusion Protein
    • GFP (N terminal on backbone)
  • par-2 CELE_F58B6.3
    40140 pFM037 par-2 RNAi resistant (R163A) (Caenorhabditis elegans) Worm Expression ; Gateway Destination vector Motegi et al Nat Cell Biol. 2011 Oct 9;13(11):1361-7. doi: 10.1038/ncb2354. pID3.01
  • Tag / Fusion Protein
    • GFP (N terminal on backbone)
  • par-2 CELE_F58B6.3
    40141 pFM038 par-2 RNAi resistant (R183-5A + 7SA) (Caenorhabditis elegans) Worm Expression ; Gateway Destination vector Motegi et al Nat Cell Biol. 2011 Oct 9;13(11):1361-7. doi: 10.1038/ncb2354. pID3.01
  • Tag / Fusion Protein
    • GFP (N terminal on backbone)
  • par-2 CELE_F58B6.3
    40142 pFM039 par-2 RNAi resistant (R183-5A + S241A) (Caenorhabditis elegans) Worm Expression ; Gateway Destination vector Motegi et al Nat Cell Biol. 2011 Oct 9;13(11):1361-7. doi: 10.1038/ncb2354. pID3.01
  • Tag / Fusion Protein
    • GFP (N terminal on backbone)
  • par-2 CELE_F58B6.3
    40144 pFM041 par-2 RNAi resistant (S241A) (Caenorhabditis elegans) Worm Expression ; Gateway Destination vector Motegi et al Nat Cell Biol. 2011 Oct 9;13(11):1361-7. doi: 10.1038/ncb2354. pID3.01
  • Tag / Fusion Protein
    • GFP (N terminal on backbone)
  • par-2 CELE_F58B6.3
    40146 pFM052 par-2 RNAi resistant (C56S) (Caenorhabditis elegans) Worm Expression ; Gateway Destination vector Motegi et al Nat Cell Biol. 2011 Oct 9;13(11):1361-7. doi: 10.1038/ncb2354. pID3.01
  • Tag / Fusion Protein
    • GFP (N terminal on backbone)
  • par-2 CELE_F58B6.3
    40147 pFM101 gfp::par-2 wild-type RNAi resistant (Caenorhabditis elegans) Bacterial Expression Motegi et al Nat Cell Biol. 2011 Oct 9;13(11):1361-7. doi: 10.1038/ncb2354. pGEX-6p-1 par-2 CELE_F58B6.3
    40148 pFM102 gfp::par-2 RNAi resistant R183-5A (Caenorhabditis elegans) Bacterial Expression Motegi et al Nat Cell Biol. 2011 Oct 9;13(11):1361-7. doi: 10.1038/ncb2354. pGEX-6p-1
  • Tags / Fusion Proteins
    • GST (N terminal on backbone)
    • eGFP (N terminal on insert)
  • par-2 CELE_F58B6.3
    40149 pFM103 par-2 RNAi resistant, wild-type (Caenorhabditis elegans) Bacterial Expression Motegi et al Nat Cell Biol. 2011 Oct 9;13(11):1361-7. doi: 10.1038/ncb2354. pGEX-6p-1
  • Tag / Fusion Protein
    • GST (N terminal on backbone)
  • par-2 CELE_F58B6.3
    40150 pFM104 par-2 RNAi resistant, R183-5A (Caenorhabditis elegans) Bacterial Expression Motegi et al Nat Cell Biol. 2011 Oct 9;13(11):1361-7. doi: 10.1038/ncb2354. pGEX-6p-1
  • Tag / Fusion Protein
    • GST (N terminal on backbone)
  • par-2 CELE_F58B6.3
    40151 pFM105 par-2 RNAi resistant, K162A (Caenorhabditis elegans) Bacterial Expression Motegi et al Nat Cell Biol. 2011 Oct 9;13(11):1361-7. doi: 10.1038/ncb2354. pGEX-6p-1
  • Tag / Fusion Protein
    • GST (N terminal on backbone)
  • par-2 CELE_F58B6.3
    40152 pFM106 par-2 RNAi resistant, R163A (Caenorhabditis elegans) Bacterial Expression Motegi et al Nat Cell Biol. 2011 Oct 9;13(11):1361-7. doi: 10.1038/ncb2354. pGEX-6p-1
  • Tag / Fusion Protein
    • GST (N terminal on backbone)
  • par-2 CELE_F58B6.3
    40153 pFM107 par-2 RNAi resistant, 7SA (Caenorhabditis elegans) Bacterial Expression Motegi et al Nat Cell Biol. 2011 Oct 9;13(11):1361-7. doi: 10.1038/ncb2354. pGEX-6p-1
  • Tag / Fusion Protein
    • GST (N terminal on backbone)
  • par-2 CELE_F58B6.3
    40154 pFM108 par-2 RNAi resistant, S241A (Caenorhabditis elegans) Bacterial Expression Motegi et al Nat Cell Biol. 2011 Oct 9;13(11):1361-7. doi: 10.1038/ncb2354. pGEX-6p-1
  • Tag / Fusion Protein
    • GST (N terminal on backbone)
  • par-2 CELE_F58B6.3
    40155 pFM109 par-2 RNAi resistant, C56S (Caenorhabditis elegans) Bacterial Expression Motegi et al Nat Cell Biol. 2011 Oct 9;13(11):1361-7. doi: 10.1038/ncb2354. pGEX-6p-1
  • Tag / Fusion Protein
    • GST (N terminal on backbone)
  • par-2 CELE_F58B6.3
    40156 pFM110 par-2 RNAi resistant, 7SE (Caenorhabditis elegans) Bacterial Expression Motegi et al Nat Cell Biol. 2011 Oct 9;13(11):1361-7. doi: 10.1038/ncb2354. pGEX-6p-1
  • Tag / Fusion Protein
    • GST (N terminal on backbone)
  • par-2 CELE_F58B6.3
    40157 pFM111 par-1N:FLAG (Caenorhabditis elegans) Bacterial Expression Motegi et al Nat Cell Biol. 2011 Oct 9;13(11):1361-7. doi: 10.1038/ncb2354. pGEX-6p-1 par-1 CELE_H39E23.1
    40158 pFM112 par-1C:FLAG (Caenorhabditis elegans) Bacterial Expression Motegi et al Nat Cell Biol. 2011 Oct 9;13(11):1361-7. doi: 10.1038/ncb2354. pGEX-6p-1
  • Tags / Fusion Proteins
    • GST (N terminal on backbone)
    • modified Flag (C terminal on insert)
  • par-1 CELE_H39E23.1
    40159 pFM113 par-3N (Caenorhabditis elegans) Bacterial Expression Motegi et al Nat Cell Biol. 2011 Oct 9;13(11):1361-7. doi: 10.1038/ncb2354. pGEX-6p-1
  • Tag / Fusion Protein
    • GST (N terminal on backbone)
  • par-3 CELE_F54E7.3 par-3 CELE_F54E7.3
    40160 pFM114 par-3N+S251A (Caenorhabditis elegans) Bacterial Expression Motegi et al Nat Cell Biol. 2011 Oct 9;13(11):1361-7. doi: 10.1038/ncb2354. pGEX-6p-1
  • Tag / Fusion Protein
    • GST (N terminal on backbone)
  • par-3 CELE_F54E7.3
    40161 pFM115 par-3C (Caenorhabditis elegans) Bacterial Expression Motegi et al Nat Cell Biol. 2011 Oct 9;13(11):1361-7. doi: 10.1038/ncb2354. pGEX-6p-1
  • Tag / Fusion Protein
    • GST (N terminal on backbone)
  • par-3 CELE_F54E7.3
    40162 pFM116 par-3C+S950A (Caenorhabditis elegans) Bacterial Expression Motegi et al Nat Cell Biol. 2011 Oct 9;13(11):1361-7. doi: 10.1038/ncb2354. pGEX-6p-1
  • Tag / Fusion Protein
    • GST (N terminal on backbone)
  • par-3 CELE_F54E7.3
    40191 pEG584 Mex-5 (RNAi resistant) (Caenorhabditis elegans) Worm Expression Griffin et al Cell. 2011 Sep 16;146(6):955-68. pCG150 mex-5 CELE_W02A2.7
    40192 pEG585 Mex-5 (S404A) (RNAi resistant) (Caenorhabditis elegans) Worm Expression Griffin et al Cell. 2011 Sep 16;146(6):955-68. pCG150
  • Tags / Fusion Proteins
    • Dendra2 PAFP (sequence optimized for worm expression; three synthetic introns inserted) (N terminal on backbone)
    • TEV protease cleavage site (N terminal on backbone)
    • S-peptide (N terminal on backbone)
  • mex-5 CELE_W02A2.7
    40193 pEG607 Mex-5 (Caenorhabditis elegans) Worm Expression Griffin et al Cell. 2011 Sep 16;146(6):955-68. pCG150
  • Tags / Fusion Proteins
    • GFP (N terminal on backbone)
    • TEV protease cleavage site (N terminal on backbone)
    • 3xFLAG (DYKDHDGDYKDHDIDYKDDDDK) (N terminal on backbone)
  • mex-5 CELE_W02A2.7
    40194 pEG608 Mex-5 (RNAi resistant) (Caenorhabditis elegans) Worm Expression Griffin et al Cell. 2011 Sep 16;146(6):955-68. pCG150
  • Tags / Fusion Proteins
    • GFP (N terminal on backbone)
    • TEV protease cleavage site (N terminal on backbone)
    • 3xFLAG (DYKDHDGDYKDHDIDYKDDDDK) (N terminal on backbone)
  • mex-5 CELE_W02A2.7
    40195 pEG610 Mex-5 (aa1-355) (RNAi resistant) (Caenorhabditis elegans) Worm Expression Griffin et al Cell. 2011 Sep 16;146(6):955-68. pCG150
  • Tags / Fusion Proteins
    • Dendra2 PAFP (sequence optimized for worm expression; three synthetic introns inserted) (N terminal on backbone)
    • TEV protease cleavage site (N terminal on backbone)
    • S-peptide (N terminal on backbone)
  • mex-5 CELE_W02A2.7
    40196 pEG587 Mex-5 (S458A) (RNAi resistant) (Caenorhabditis elegans) Worm Expression Griffin et al Cell. 2011 Sep 16;146(6):955-68. pCG150
  • Tags / Fusion Proteins
    • Dendra2 PAFP (sequence optimized for worm expression; three synthetic introns inserted) (N terminal on backbone)
    • TEV protease cleavage site (N terminal on backbone)
    • S-peptide (N terminal on backbone)
  • mex-5 CELE_W02A2.7
    40198 pEG476 Mex-5 (S404A) (Caenorhabditis elegans) Worm Expression Griffin et al Cell. 2011 Sep 16;146(6):955-68. pIC26
  • Tags / Fusion Proteins
    • Dendra2 PAFP (sequence optimized for worm expression; three synthetic introns inserted) (N terminal on backbone)
    • TEV protease cleavage site (N terminal on backbone)
    • S-peptide (N terminal on backbone)
  • mex-5 CELE_W02A2.7