Hans Clevers Lab Plasmids


The Hans Clevers Lab has deposited plasmids at Addgene for distribution to the research community. Addgene is a non-profit plasmid repository dedicated to improving life science research.

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Title Authors Publication
Controlled gene expression in primary Lgr5 organoid cultures. Koo BK, Stange DE, Sato T, Karthaus W, Farin HF, Huch M, van Es JH, Clevers H Nat Methods. 2011 Dec 4. doi: 10.1038/nmeth.1802.
ID Plasmid Gene/Insert Vector Type Publication Hidden Extra Search Info  
32702 pMSCV-loxp-dsRed-loxp-eGFP-Puro-WPRE eGFP Mammalian Expression, Retroviral, Cre/Lox Koo et al Nat Methods. 2011 Dec 4. doi: 10.1038/nmeth.1802. Conditional overexpression vector. Deletion of dsRed by Cre recombinase results in the rapid loss of dsRed and the activation of your gene fused to eGFP expression. MSCV
32703 pMSCV-loxp-dsRed-loxp-3xHA-Puro-WPRE Mammalian Expression, Retroviral, Cre/Lox Koo et al Nat Methods. 2011 Dec 4. doi: 10.1038/nmeth.1802. MSCV
  • Tag / Fusion Protein
    • 3x HA tags (C terminal on backbone)
  • 32704 pMSCV-FLIP-puro-dsRed-GFP-miRNA emGFP-miRNA Mammalian Expression, Retroviral, RNAi, Cre/Lox Koo et al Nat Methods. 2011 Dec 4. doi: 10.1038/nmeth.1802. MSCV