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Huimin Zhao Lab Plasmids


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Title Authors Publication
Modular assembly of designer PUF proteins for specific post-transcriptional regulation of endogenous RNA. Abil Z, Denard CA, Zhao H J Biol Eng. 2014 Mar 1;8(1):7.
Homology-Integrated CRISPR-Cas (HI-CRISPR) System for One-Step Multigene Disruption in Saccharomyces cerevisiae. Bao Z, Xiao H, Liang J, Zhang L, Xiong X, Sun N, Si T, Zhao H ACS Synth Biol. 2014 Sep 19.
Further improvement of phosphite dehydrogenase thermostability by saturation mutagenesis. McLachlan MJ, Johannes TW, Zhao H Biotechnol Bioeng. 2008 Feb 1;99(2):268-74.
Efficient regeneration of NADPH using an engineered phosphite dehydrogenase. Johannes TW, Woodyer RD, Zhao H Biotechnol Bioeng. 2007 Jan 1;96(1):18-26.
A single-chain TALEN architecture for genome engineering. Sun N, Zhao H Mol Biosyst. 2014 Mar 4;10(3):446-53. doi: 10.1039/c3mb70412b. Epub 2013 Dec 16.
High-Efficiency Multiplex Genome Editing of Streptomyces Species Using an Engineered CRISPR/Cas System. Cobb RE, Wang Y, Zhao H ACS Synth Biol. 2014 Dec 8.
A highly sensitive selection method for directed evolution of homing endonucleases. Chen Z, Zhao H Nucleic Acids Res. 2005 Oct 6;33(18):e154.
ID Kit Hidden Extra Search Info  
1000000051PUF Assembly Kit Add to Cart
ID Plasmid Gene/Insert Vector Type Publication Hidden Extra Search Info  
52136pChlr-pNEB193Bacterial ExpressionModular assembly of designer PUF proteins for specific post-transcriptional regulation of endogenous RNA. J Biol Eng. 2014 Mar 1;8(1):7. A backbone plasmid for creation of additional PUF repeat modules pNEB193 Add to Cart
52137pET28-GG-PUFBacterial ExpressionModular assembly of designer PUF proteins for specific post-transcriptional regulation of endogenous RNA. J Biol Eng. 2014 Mar 1;8(1):7. Receiving vector for PUM-HD assembly and expression in E.coli pET28a
  • Tags / Fusion Proteins
    • PUM-HD flanking region 1' (N terminal on insert)
    • PUM-HD flanking region 8' (C terminal on insert)
  • Add to Cart
    52138pCMV-TTP(C147R)-GG-PUFMammalian ExpressionModular assembly of designer PUF proteins for specific post-transcriptional regulation of endogenous RNA. J Biol Eng. 2014 Mar 1;8(1):7. Receiving vector for PUM-HD assembly and expression in mammalian cells pCMV5
  • Tags / Fusion Proteins
    • 3xFlag (N terminal on insert)
    • Tristetraprolin C147R (N terminal on insert)
    • (GGGGS)3GGGG linker (N terminal on insert)
    • PUM-HD flanking region 1' (N terminal on backbone)
    • PUM-HD flanking region 8' (C terminal on backbone)
  • Add to Cart
    52139pChlr-1SQPumilio homology domain amino acids 25-60 (Homo sapiens)Bacterial ExpressionModular assembly of designer PUF proteins for specific post-transcriptional regulation of endogenous RNA. J Biol Eng. 2014 Mar 1;8(1):7. Encodes module 1 with amino acids 12S-16Q for recognition of nt 8A pChlr-pNEB193 PUM1 HSPUM, PUMH, PUMH1, PUML1 Add to Cart
    52140pChlr-1SEPumilio homology domain amino acids 25-60 (Homo sapiens)Bacterial ExpressionModular assembly of designer PUF proteins for specific post-transcriptional regulation of endogenous RNA. J Biol Eng. 2014 Mar 1;8(1):7. Encodes module 1 with amino acids 12S-16E for recognition of nt 8G pChlr-pNEB193 PUM1 HSPUM, PUMH, PUMH1, PUML1 Add to Cart
    52141pChlr-1NQPumilio homology domain amino acids 25-60 (Homo sapiens)Bacterial ExpressionModular assembly of designer PUF proteins for specific post-transcriptional regulation of endogenous RNA. J Biol Eng. 2014 Mar 1;8(1):7. Encodes module 1 with amino acids 12N-16Q for recognition of nt 8U pChlr-pNEB193 PUM1 HSPUM, PUMH, PUMH1, PUML1 Add to Cart
    52142pChlr-1SRPumilio homology domain amino acids 25-60 (Homo sapiens)Bacterial ExpressionModular assembly of designer PUF proteins for specific post-transcriptional regulation of endogenous RNA. J Biol Eng. 2014 Mar 1;8(1):7. Encodes module 1 with amino acids 12S-16R for recognition of nt 8C pChlr-pNEB193 PUM1 HSPUM, PUMH, PUMH1, PUML1 Add to Cart
    52143pChlr-2CQPumilio homology domain amino acids 61-96 (Homo sapiens)Bacterial ExpressionModular assembly of designer PUF proteins for specific post-transcriptional regulation of endogenous RNA. J Biol Eng. 2014 Mar 1;8(1):7. Encodes module 2 with amino acids 12C-16Q for recognition of nt 7A pChlr-pNEB193 PUM1 HSPUM, PUMH, PUMH1, PUML1 Add to Cart
    52144pChlr-2SEPumilio homology domain amino acids 61-96 (Homo sapiens)Bacterial ExpressionModular assembly of designer PUF proteins for specific post-transcriptional regulation of endogenous RNA. J Biol Eng. 2014 Mar 1;8(1):7. Encodes module 2 with amino acids 12S-16E for recognition of nt 7G pChlr-pNEB193 PUM1 HSPUM, PUMH, PUMH1, PUML1 Add to Cart
    52145pChlr-2NQPumilio homology domain amino acids 61-96 (Homo sapiens)Bacterial ExpressionModular assembly of designer PUF proteins for specific post-transcriptional regulation of endogenous RNA. J Biol Eng. 2014 Mar 1;8(1):7. Encodes module 2 with amino acids 12N-16Q for recognition of nt 7U pChlr-pNEB193 PUM1 HSPUM, PUMH, PUMH1, PUML1 Add to Cart
    52146pChlr-2SRPumilio homology domain amino acids 61-96 (Homo sapiens)Bacterial ExpressionModular assembly of designer PUF proteins for specific post-transcriptional regulation of endogenous RNA. J Biol Eng. 2014 Mar 1;8(1):7. Encodes module 2 with amino acids 12S-16R for recognition of nt 7C pChlr-pNEB193 PUM1 HSPUM, PUMH, PUMH1, PUML1 Add to Cart
    52147pChlr-3CQPumilio homology domain amino acids 97-132 (Homo sapiens)Bacterial ExpressionModular assembly of designer PUF proteins for specific post-transcriptional regulation of endogenous RNA. J Biol Eng. 2014 Mar 1;8(1):7. Encodes module 3 with amino acids 12C-16Q for recognition of nt 6A pChlr-pNEB193 PUM1 HSPUM, PUMH, PUMH1, PUML1 Add to Cart
    52148pChlr-3SEPumilio homology domain amino acids 97-132 (Homo sapiens)Bacterial ExpressionModular assembly of designer PUF proteins for specific post-transcriptional regulation of endogenous RNA. J Biol Eng. 2014 Mar 1;8(1):7. Encodes module 3 with amino acids 12S-16E for recognition of nt 6G pChlr-pNEB193 PUM1 HSPUM, PUMH, PUMH1, PUML1 Add to Cart
    52149pChlr-3NQPumilio homology domain amino acids 97-132 (Homo sapiens)Bacterial ExpressionModular assembly of designer PUF proteins for specific post-transcriptional regulation of endogenous RNA. J Biol Eng. 2014 Mar 1;8(1):7. Encodes module 3 with amino acids 12N-16Q for recognition of nt 6U pChlr-pNEB193 PUM1 HSPUM, PUMH, PUMH1, PUML1 Add to Cart
    52150pChlr-3SRPumilio homology domain amino acids 97-132 (Homo sapiens)Bacterial ExpressionModular assembly of designer PUF proteins for specific post-transcriptional regulation of endogenous RNA. J Biol Eng. 2014 Mar 1;8(1):7. Encodes module 3 with amino acids 12S-16R for recognition of nt 6C pChlr-pNEB193 PUM1 HSPUM, PUMH, PUMH1, PUML1 Add to Cart
    52151pChlr-4CQPumilio homology domain amino acids 133-168 (Homo sapiens)Bacterial ExpressionModular assembly of designer PUF proteins for specific post-transcriptional regulation of endogenous RNA. J Biol Eng. 2014 Mar 1;8(1):7. Encodes module 4 with amino acids 12C-16Q for recognition of nt 5A pChlr-pNEB193 PUM1 HSPUM, PUMH, PUMH1, PUML1 Add to Cart
    52152pChlr-4SEPumilio homology domain amino acids 133-168 (Homo sapiens)Bacterial ExpressionModular assembly of designer PUF proteins for specific post-transcriptional regulation of endogenous RNA. J Biol Eng. 2014 Mar 1;8(1):7. Encodes module 4 with amino acids 12S-16E for recognition of nt 5G pChlr-pNEB193 PUM1 HSPUM, PUMH, PUMH1, PUML1 Add to Cart
    52153pChlr-4NQPumilio homology domain amino acids 133-168 (Homo sapiens)Bacterial ExpressionModular assembly of designer PUF proteins for specific post-transcriptional regulation of endogenous RNA. J Biol Eng. 2014 Mar 1;8(1):7. Encodes module 4 with amino acids 12N-16Q for recognition of nt 5U pChlr-pNEB193 PUM1 HSPUM, PUMH, PUMH1, PUML1 Add to Cart
    52154pChlr-4SRPumilio homology domain amino acids 133-168 (Homo sapiens)Bacterial ExpressionModular assembly of designer PUF proteins for specific post-transcriptional regulation of endogenous RNA. J Biol Eng. 2014 Mar 1;8(1):7. Encodes module 4 with amino acids 12S-16R for recognition of nt 5C pChlr-pNEB193 PUM1 HSPUM, PUMH, PUMH1, PUML1 Add to Cart
    52155pChlr-4CYQPumilio homology domain amino acids 133-168 (Homo sapiens)Bacterial ExpressionModular assembly of designer PUF proteins for specific post-transcriptional regulation of endogenous RNA. J Biol Eng. 2014 Mar 1;8(1):7. Encodes module 4 with amino acids 12C-13Y-16Q for recognition of nt 5A pChlr-pNEB193 PUM1 HSPUM, PUMH, PUMH1, PUML1 Add to Cart
    52156pChlr-4SYED43951 (Homo sapiens)Bacterial ExpressionModular assembly of designer PUF proteins for specific post-transcriptional regulation of endogenous RNA. J Biol Eng. 2014 Mar 1;8(1):7. Encodes module 4 with amino acids 12S-13Y-16E for recognition of nt 5G pChlr-pNEB193 PUM1 HSPUM, PUMH, PUMH1, PUML1 Add to Cart
    52157pChlr-4NYQPumilio homology domain amino acids 133-168 (Homo sapiens)Bacterial ExpressionModular assembly of designer PUF proteins for specific post-transcriptional regulation of endogenous RNA. J Biol Eng. 2014 Mar 1;8(1):7. Encodes module 4 with amino acids 12N-13Y-16Q for recognition of nt 5U pChlr-pNEB193 PUM1 HSPUM, PUMH, PUMH1, PUML1 Add to Cart
    52158pChlr-4SYRPumilio homology domain amino acids 133-168 (Homo sapiens)Bacterial ExpressionModular assembly of designer PUF proteins for specific post-transcriptional regulation of endogenous RNA. J Biol Eng. 2014 Mar 1;8(1):7. Encodes module 4 with amino acids 12S-13Y-16R for recognition of nt 5C pChlr-pNEB193 PUM1 HSPUM, PUMH, PUMH1, PUML1 Add to Cart
    52159pChlr-5CQPumilio homology domain amino acids 169-204 (Homo sapiens)Bacterial ExpressionModular assembly of designer PUF proteins for specific post-transcriptional regulation of endogenous RNA. J Biol Eng. 2014 Mar 1;8(1):7. Encodes module 5 with amino acids 12C-16Q for recognition of nt 4A pChlr-pNEB193 PUM1 HSPUM, PUMH, PUMH1, PUML1 Add to Cart
    52160pChlr-5SEPumilio homology domain amino acids 169-204 (Homo sapiens)Bacterial ExpressionModular assembly of designer PUF proteins for specific post-transcriptional regulation of endogenous RNA. J Biol Eng. 2014 Mar 1;8(1):7. Encodes module 5 with amino acids 12S-16E for recognition of nt 4G pChlr-pNEB193 PUM1 HSPUM, PUMH, PUMH1, PUML1 Add to Cart
    52161pChlr-5NQPumilio homology domain amino acids 169-204 (Homo sapiens)Bacterial ExpressionModular assembly of designer PUF proteins for specific post-transcriptional regulation of endogenous RNA. J Biol Eng. 2014 Mar 1;8(1):7. Encodes module 5 with amino acids 12N-16Q for recognition of nt 4U pChlr-pNEB193 PUM1 HSPUM, PUMH, PUMH1, PUML1 Add to Cart
    52162pChlr-5SRPumilio homology domain amino acids 169-204 (Homo sapiens)Bacterial ExpressionModular assembly of designer PUF proteins for specific post-transcriptional regulation of endogenous RNA. J Biol Eng. 2014 Mar 1;8(1):7. Encodes module 5 with amino acids 12S-16R for recognition of nt 4C pChlr-pNEB193 PUM1 HSPUM, PUMH, PUMH1, PUML1 Add to Cart
    52163pChlr-6CQPumilio homology domain amino acids 205-240 (Homo sapiens)Bacterial ExpressionModular assembly of designer PUF proteins for specific post-transcriptional regulation of endogenous RNA. J Biol Eng. 2014 Mar 1;8(1):7. Encodes module 6 with amino acids 12C-16Q for recognition of nt 3A pChlr-pNEB193 PUM1 HSPUM, PUMH, PUMH1, PUML1 Add to Cart
    52164pChlr-6SEPumilio homology domain amino acids 205-240 (Homo sapiens)Bacterial ExpressionModular assembly of designer PUF proteins for specific post-transcriptional regulation of endogenous RNA. J Biol Eng. 2014 Mar 1;8(1):7. Encodes module 6 with amino acids 12S-16E for recognition of nt 3G pChlr-pNEB193 PUM1 HSPUM, PUMH, PUMH1, PUML1 Add to Cart
    52165pChlr-6NQPumilio homology domain amino acids 205-240 (Homo sapiens)Bacterial ExpressionModular assembly of designer PUF proteins for specific post-transcriptional regulation of endogenous RNA. J Biol Eng. 2014 Mar 1;8(1):7. Encodes module 6 with amino acids 12N-16Q for recognition of nt 3U pChlr-pNEB193 PUM1 HSPUM, PUMH, PUMH1, PUML1 Add to Cart
    52166pChlr-6SRPumilio homology domain amino acids 205-240 (Homo sapiens)Bacterial ExpressionModular assembly of designer PUF proteins for specific post-transcriptional regulation of endogenous RNA. J Biol Eng. 2014 Mar 1;8(1):7. Encodes module 6 with amino acids 12S-16R for recognition of nt 3C pChlr-pNEB193 PUM1 HSPUM, PUMH, PUMH1, PUML1 Add to Cart
    52167pChlr-7CQPumilio homology domain amino acids 241-283 (Homo sapiens)Bacterial ExpressionModular assembly of designer PUF proteins for specific post-transcriptional regulation of endogenous RNA. J Biol Eng. 2014 Mar 1;8(1):7. Encodes module 7 with amino acids 12C-16Q for recognition of nt 2A pChlr-pNEB193 PUM1 HSPUM, PUMH, PUMH1, PUML1 Add to Cart
    52168pChlr-7SEPumilio homology domain amino acids 241-283 (Homo sapiens)Bacterial ExpressionModular assembly of designer PUF proteins for specific post-transcriptional regulation of endogenous RNA. J Biol Eng. 2014 Mar 1;8(1):7. Encodes module 7 with amino acids 12S-16E for recognition of nt 2G pChlr-pNEB193 PUM1 HSPUM, PUMH, PUMH1, PUML1 Add to Cart
    52169pChlr-7NQPumilio homology domain amino acids 241-283 (Homo sapiens)Bacterial ExpressionModular assembly of designer PUF proteins for specific post-transcriptional regulation of endogenous RNA. J Biol Eng. 2014 Mar 1;8(1):7. Encodes module 7 with amino acids 12N-16Q for recognition of nt 2U pChlr-pNEB193 PUM1 HSPUM, PUMH, PUMH1, PUML1 Add to Cart
    52170pChlr-7SYRPumilio homology domain amino acids 241-283 (Homo sapiens)Bacterial ExpressionModular assembly of designer PUF proteins for specific post-transcriptional regulation of endogenous RNA. J Biol Eng. 2014 Mar 1;8(1):7. Encodes module 7 with amino acids 12S-13Y-16R for recognition of nt 2C pChlr-pNEB193 PUM1 HSPUM, PUMH, PUMH1, PUML1 Add to Cart
    52171pChlr-8CQPumilio homology domain amino acids 284-319 (Homo sapiens)Bacterial ExpressionModular assembly of designer PUF proteins for specific post-transcriptional regulation of endogenous RNA. J Biol Eng. 2014 Mar 1;8(1):7. Encodes module 8 with amino acids 12C-16Q for recognition of nt 1A pChlr-pNEB193 PUM1 HSPUM, PUMH, PUMH1, PUML1 Add to Cart
    52172pChlr-8SEPumilio homology domain amino acids 284-319 (Homo sapiens)Bacterial ExpressionModular assembly of designer PUF proteins for specific post-transcriptional regulation of endogenous RNA. J Biol Eng. 2014 Mar 1;8(1):7. Encodes module 8 with amino acids 12S-16E for recognition of nt 1G pChlr-pNEB193 PUM1 HSPUM, PUMH, PUMH1, PUML1 Add to Cart
    52173pChlr-8NQPumilio homology domain amino acids 284-319 (Homo sapiens)Bacterial ExpressionModular assembly of designer PUF proteins for specific post-transcriptional regulation of endogenous RNA. J Biol Eng. 2014 Mar 1;8(1):7. Encodes module 8 with amino acids 12N-16Q for recognition of nt 1U pChlr-pNEB193 PUM1 HSPUM, PUMH, PUMH1, PUML1 Add to Cart
    52174pChlr-8SRPumilio homology domain amino acids 284-319 (Homo sapiens)Bacterial ExpressionModular assembly of designer PUF proteins for specific post-transcriptional regulation of endogenous RNA. J Biol Eng. 2014 Mar 1;8(1):7. Encodes module 8 with amino acids 12S-16R for recognition of nt 1C pChlr-pNEB193 Add to Cart
    60619pCRYeast Expression, CRISPRHomology-Integrated CRISPR-Cas (HI-CRISPR) System for One-Step Multigene Disruption in Saccharomyces cerevisiae. ACS Synth Biol. 2014 Sep 19. crRNA expression plasmid on pRS423 backbone pRS423 Add to Cart
    60620pCTiCas9 (Other), tracrRNA (Other)Yeast Expression, CRISPRHomology-Integrated CRISPR-Cas (HI-CRISPR) System for One-Step Multigene Disruption in Saccharomyces cerevisiae. ACS Synth Biol. 2014 Sep 19. Plasmid encoding iCas9 and tracrRNA on pRS415 backbone pRS415 Add to Cart
    60621pCRCTiCas9 (Other), tracrRNA (Other)Yeast Expression, CRISPRHomology-Integrated CRISPR-Cas (HI-CRISPR) System for One-Step Multigene Disruption in Saccharomyces cerevisiae. ACS Synth Biol. 2014 Sep 19. Plasmid encoding iCas9, tracrRNA and crRNAs pRS426 Add to Cart
    61695pCMVJ-scTALEN-ABL1-RscTALEN-ABL1-R (Synthetic)Mammalian ExpressionA single-chain TALEN architecture for genome engineering. Mol Biosyst. 2014 Mar 4;10(3):446-53. doi: 10.1039/c3mb70412b. Epub 2013 Dec 16. Expresses a single-chain TALEN targeting ABL1 site in human cells pCMV5 Add to Cart
    61697pET15b-Opt12Opt12 (Other)Bacterial ExpressionFurther improvement of phosphite dehydrogenase thermostability by saturation mutagenesis. Biotechnol Bioeng. 2008 Feb 1;99(2):268-74. Expresses PTDH mutant Opt12 pET15b Add to Cart
    61698pET15b-Opt13Opt13 (Other)Bacterial ExpressionFurther improvement of phosphite dehydrogenase thermostability by saturation mutagenesis. Biotechnol Bioeng. 2008 Feb 1;99(2):268-74. Expresses PTDH mutant Opt13 pET15b Add to Cart
    61699pET15b-12xOpt12x (Other)Bacterial ExpressionEfficient regeneration of NADPH using an engineered phosphite dehydrogenase. Biotechnol Bioeng. 2007 Jan 1;96(1):18-26. Expresses PTDH mutant Opt12x pET15b Add to Cart
    61736pCRISPomyces-1sSpCas9 (Synthetic), tracrRNA (Other), crRNA cassette (Synthetic)Bacterial Expression, CRISPRHigh-Efficiency Multiplex Genome Editing of Streptomyces Species Using an Engineered CRISPR/Cas System. ACS Synth Biol. 2014 Dec 8. Streptomyces expression of codon-optimized Cas9, tracrRNA, and custom crRNA pKC1139 Add to Cart
    61737pCRISPomyces-2sSpCas9 (Synthetic), gRNA cassette (Synthetic)Bacterial Expression, CRISPRHigh-Efficiency Multiplex Genome Editing of Streptomyces Species Using an Engineered CRISPR/Cas System. ACS Synth Biol. 2014 Dec 8. Streptomyces expression of codon-optimized Cas9 and custom gRNA pKC1139 Add to Cart
    69056p11-LacY-wtx1ccdB, lacYA highly sensitive selection method for directed evolution of homing endonucleases. Nucleic Acids Res. 2005 Oct 6;33(18):e154. The reporter plasmid p11-LacY-wtx1 encodes the toxin CcdB gene under the inducible BAD promoter and a single copy of the endonuclease cleavage site which is readily extendable to multiple copies. pBAD18 ccdB pCoo051 lacY b0343, ECK0340, JW0334 Add to Cart