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Iskra Katic Lab Plasmids


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Title Authors Publication
Targeted Heritable Mutation and Gene Conversion by Cas9-CRISPR in Caenorhabditis elegans. Katic I, Grosshans H Genetics. 2013 Aug 26.
CRISPR/Cas9 Genome Editing in Caenorhabditis elegans: Evaluation of Templates for Homology-Mediated Repair and Knock-Ins by Homology-Independent DNA Repair. Katic I, Xu L, Ciosk R G3 (Bethesda). 2015 Jun 3. pii: g3.115.019273. doi: 10.1534/g3.115.019273.
ID Plasmid Gene/Insert Vector Type Publication Hidden Extra Search Info  
47933pIK86Cas9 (Synthetic)CRISPR, UnspecifiedTargeted Heritable Mutation and Gene Conversion by Cas9-CRISPR in Caenorhabditis elegans. Genetics. 2013 Aug 26. C. elegans codon-optimized Cas9 with 2xSV40 NLS sequences. It has a T7 promoter for in vitro transcription, germline-compatible 5'UTR, tbb-2 3'UTR and a polyA sequence. pUC57 Add to Cart
65629pIK198Worm Expression, CRISPRCRISPR/Cas9 Genome Editing in Caenorhabditis elegans: Evaluation of Templates for Homology-Mediated Repair and Knock-Ins by Homology-Independent DNA Repair. G3 (Bethesda). 2015 Jun 3. pii: g3.115.019273. doi: 10.1534/g3.115.019273. "flipped and extended" sgRNA backbone (Chen et al., 2013) following a C. elegans U6 promoter (Friedland et al., 2013). For Gibson cloning locus-specific sgRNA sequences. pUC57 Add to Cart
65630pIK208U6 promoter::dpy-10 sgRNA with a "flipped and extended" sgRNA backbone (Caenorhabditis elegans)Worm Expression, CRISPRCRISPR/Cas9 Genome Editing in Caenorhabditis elegans: Evaluation of Templates for Homology-Mediated Repair and Knock-Ins by Homology-Independent DNA Repair. G3 (Bethesda). 2015 Jun 3. pii: g3.115.019273. doi: 10.1534/g3.115.019273. sgRNA targeting dpy-10 (Arribere et al., 2014) expressed from a C. elegans U6 promoter (Friedland et al., 2013), in a "flipped and extended" sgRNA backbone (Chen et al., 2013)" pUC57 Add to Cart
65631pIK127Peft-3::gfp::h2b::tbb-2 3'UTR (Caenorhabditis elegans)Worm ExpressionCRISPR/Cas9 Genome Editing in Caenorhabditis elegans: Evaluation of Templates for Homology-Mediated Repair and Knock-Ins by Homology-Independent DNA Repair. G3 (Bethesda). 2015 Jun 3. pii: g3.115.019273. doi: 10.1534/g3.115.019273. Expresses gfp::h2b in C. elegans cells from the ubiquitous eef-1A.1 (eft-3) promoter. pUC57 Add to Cart
65632pIK137Peft-3::gfp::h2b::tbb-2 3'UTR, C. briggsae unc-119 rescue fragment (Caenorhabditis elegans)Worm ExpressionCRISPR/Cas9 Genome Editing in Caenorhabditis elegans: Evaluation of Templates for Homology-Mediated Repair and Knock-Ins by Homology-Independent DNA Repair. G3 (Bethesda). 2015 Jun 3. pii: g3.115.019273. doi: 10.1534/g3.115.019273. Expresses gfp::h2b in C. elegans cells from the ubiquitous eef-1A.1 (eft-3) promoter and contains a C. briggsae unc-119 rescuing fragment pUC57 Add to Cart