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James Thomson Lab Plasmids


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Title Authors Publication
Feeder-independent culture of human embryonic stem cells. Ludwig TE, Bergendahl V, Levenstein ME, Yu J, Probasco MD, Thomson JA Nat Methods. 2006 Aug . 3(8):637-646.
Induced Pluripotent Stem Cell Lines Derived from Human Somatic Cells. Yu J, Vodyanik MA, Smuga-Otto K, Antosiewicz-Bourget J, Frane JL, Tian S, Nie J, Jonsdottir GA, Ruotti V, Stewart R, Slukvin II, Thomson JA Science. 2007 Nov 20. ():.
Human Induced Pluripotent Stem Cells Free of Vector and Transgene Sequences Yu J, Hu K, Smuga-Otto K, Tian S, Stewart R, Slukvin II, Thomson JA Science. 2009 Mar 26.
Homologous recombination in human embryonic stem cells. Zwaka TP, Thomson JA Nat Biotechnol. 2003 Mar . 21(3):319-21.
Chemically defined conditions for human iPSC derivation and culture. Chen G, Gulbranson DR, Hou Z, Bolin JM, Ruotti V, Probasco MD, Smuga-Otto K, Howden SE, Diol NR, Propson NE, Wagner R, Lee GO, Antosiewicz-Bourget J, Teng JM, Thomson JA Nat Methods. 2011 Apr 10. ():.
Phosphorylation regulates human OCT4. Brumbaugh J, Hou Z, Russell JD, Howden SE, Yu P, Ledvina AR, Coon JJ, Thomson JA Proc Natl Acad Sci U S A. 2012 May 8;109(19):7162-8. Epub 2012 Apr 2.
Efficient genome engineering in human pluripotent stem cells using Cas9 from Neisseria meningitidis. Hou Z, Zhang Y, Propson NE, Howden SE, Chu LF, Sontheimer EJ, Thomson JA Proc Natl Acad Sci U S A. 2013 Aug 12.
Thomson/Sontheimer Lab NmCas9 plasmids (unpublished)
An expandable, inducible hemangioblast state regulated by fibroblast growth factor. Vereide DT, Vickerman V, Swanson SA, Chu LF, McIntosh BE, Thomson JA Stem Cell Reports. 2014 Dec 9;3(6):1043-57. doi: 10.1016/j.stemcr.2014.10.003. Epub 2014 Nov 13.
Single-cell RNA-seq reveals novel regulators of human embryonic stem cell differentiation to definitive endoderm. Chu LF, Leng N, Zhang J, Hou Z, Mamott D, Vereide DT, Choi J, Kendziorski C, Stewart R, Thomson JA Genome Biol. 2016 Aug 17;17(1):173. doi: 10.1186/s13059-016-1033-x.
Simultaneous Reprogramming and Gene Correction of Patient Fibroblasts. Howden SE, Maufort JP, Duffin BM, Elefanty AG, Stanley EG, Thomson JA Stem Cell Reports. 2015 Dec 8;5(6):1109-18. doi: 10.1016/j.stemcr.2015.10.009. Epub 2015 Nov 12.
ID Plasmid Gene/Insert Vector Type Publication Hidden Extra Search Info  
12309zbFGFFgf2 (Danio rerio)Bacterial ExpressionFeeder-independent culture of human embryonic stem cells. Nat Methods. 2006 Aug . 3(8):637-646. pET28b(+) fgf2 Add to Cart
16350pGEM-LIN28Homo sapiens lin-28 homolog (C. elegans) (Homo sapiens)Bacterial ExpressionInduced Pluripotent Stem Cell Lines Derived from Human Somatic Cells. Science. 2007 Nov 20. ():. pGEM-T Easy LIN28A CSDD1, LIN-28, LIN28, ZCCHC1, lin-28A Add to Cart
16351pGEM-NanogHomo sapiens Nanog homeobox (Homo sapiens)Bacterial ExpressionInduced Pluripotent Stem Cell Lines Derived from Human Somatic Cells. Science. 2007 Nov 20. ():. pGEM-T Easy NANOG Add to Cart
16352pGEM-Oct4Homo sapiens POU domain, class 5, transcription factor 1 (POU5F1) (Homo sapiens)Bacterial ExpressionInduced Pluripotent Stem Cell Lines Derived from Human Somatic Cells. Science. 2007 Nov 20. ():. pGEM-T Easy POU5F1 OCT3, OCT4, OTF-3, OTF3, OTF4, Oct-3, Oct-4 Add to Cart
16353pGEM-Sox2Homo sapiens SRY (sex determining region Y)-box 2 (SOX2) (Homo sapiens)Bacterial ExpressionInduced Pluripotent Stem Cell Lines Derived from Human Somatic Cells. Science. 2007 Nov 20. ():. pGEM-T Easy SOX2 ANOP3, MCOPS3 Add to Cart
16577pSin-EF2-Sox2-PurHomo sapiens SRY (sex determining region Y)-box 2 (SOX2) (Homo sapiens)Mammalian Expression, LentiviralInduced Pluripotent Stem Cell Lines Derived from Human Somatic Cells. Science. 2007 Nov 20. ():. pSin4-EF2-IRES-Pur SOX2 ANOP3, MCOPS3 Add to Cart
16578pSin-EF2-Nanog-PurHomo sapiens Nanog homeobox (Homo sapiens)Mammalian Expression, LentiviralInduced Pluripotent Stem Cell Lines Derived from Human Somatic Cells. Science. 2007 Nov 20. ():. pSin4-EF2-IRES-Pur NANOG Add to Cart
16579pSin-EF2-Oct4-PurHomo sapiens POU domain, class 5, transcription factor 1 (POU5F1) (Homo sapiens)Mammalian Expression, LentiviralInduced Pluripotent Stem Cell Lines Derived from Human Somatic Cells. Science. 2007 Nov 20. ():. pSin4-EF2-IRES-Pur POU5F1 OCT3, OCT4, OTF-3, OTF3, OTF4, Oct-3, Oct-4 Add to Cart
16580pSin-EF2-LIN28-PurHomo sapiens lin-28 homolog (C. elegans) (Homo sapiens)Mammalian Expression, LentiviralInduced Pluripotent Stem Cell Lines Derived from Human Somatic Cells. Science. 2007 Nov 20. ():. pSin4-EF2-IRES-Pur LIN28A CSDD1, LIN-28, LIN28, ZCCHC1, lin-28A Add to Cart
20922pEP4 E02S EN2LOct4 and Sox2; Nanog and Lin28 (Homo sapiens)Episomal/EBNAHuman Induced Pluripotent Stem Cells Free of Vector and Transgene Sequences Science. 2009 Mar 26. used in the derivation of human iPS cells using non-integrating episomal vectors; expresses Oct4 and Sox2; Nanog and Lin28 pCEP4 POU5F1 OCT3, OCT4, OTF-3, OTF3, OTF4, Oct-3, Oct-4 SOX2 ANOP3, MCOPS3 NANOG LIN28A CSDD1, LIN-28, LIN28, ZCCHC1, lin-28A Add to Cart
20923pEP4 E02S EM2KOct4 and Sox2; Myc and Klf4 (Homo sapiens)Episomal/EBNAHuman Induced Pluripotent Stem Cells Free of Vector and Transgene Sequences Science. 2009 Mar 26. used in the derivation of human iPS cells using non-integrating episomal vectors; expresses Oct4 and Sox2; Myc and Klf4 pCEP4 SOX2 ANOP3, MCOPS3 KLF4 EZF, GKLF MYC MRTL, MYCC, bHLHe39, c-Myc Add to Cart
20924pEP4 E02S CK2M EN2LOct4 and Sox2; Nanog and Lin28; Klf4 and Myc (Homo sapiens)Episomal/EBNAHuman Induced Pluripotent Stem Cells Free of Vector and Transgene Sequences Science. 2009 Mar 26. used in the derivation of human iPS cells using non-integrating episomal vectors; expresses Oct4 and Sox2; Klf4 and Myc; Nanog and Lin28 pCEP4 MYC MRTL, MYCC, bHLHe39, c-Myc Add to Cart
20925pEP4 E02S EN2KOct4 and Sox2; Nanog and Klf4 (Homo sapiens)Episomal/EBNAHuman Induced Pluripotent Stem Cells Free of Vector and Transgene Sequences Science. 2009 Mar 26. used in the derivation of human iPS cells using non-integrating episomal vectors; expresses Oct4 and Sox2; Nanog and Klf4 pCEP4 SOX2 ANOP3, MCOPS3 POU5F1 OCT3, OCT4, OTF-3, OTF3, OTF4, Oct-3, Oct-4 Add to Cart
20926pCEP4-M2LMYC (Homo sapiens), LIN28 (Homo sapiens)Episomal/EBNAHuman Induced Pluripotent Stem Cells Free of Vector and Transgene Sequences Science. 2009 Mar 26. pCEP4 MYC MRTL, MYCC, bHLHe39, c-Myc LIN28A CSDD1, LIN-28, LIN28, ZCCHC1, lin-28A Add to Cart
20927pEP4 E02S ET2KOct4 and Sox2; SV40LT and Klf4 (Homo sapiens)Episomal/EBNAHuman Induced Pluripotent Stem Cells Free of Vector and Transgene Sequences Science. 2009 Mar 26. used in the derivation of human iPS cells using non-integrating episomal vectors; expresses Oct4 and Sox2; SV40LT and Klf4 pCEP4 SOX2 ANOP3, MCOPS3 POU5F1 OCT3, OCT4, OTF-3, OTF3, OTF4, Oct-3, Oct-4 KLF4 EZF, GKLF Add to Cart
21162pSIN4-EF2-O2SOct4, Sox2 (Homo sapiens)Lentiviral ; reprogrammingHuman Induced Pluripotent Stem Cells Free of Vector and Transgene Sequences Science. 2009 Mar 26. pSIN4-EF2-IRES-Pur SOX2 ANOP3, MCOPS3 POU5F1 OCT3, OCT4, OTF-3, OTF3, OTF4, Oct-3, Oct-4 Add to Cart
21163pSIN4-EF2-N2LNanog, Lin28 (Homo sapiens)Mammalian Expression, Lentiviral ; reprogrammingHuman Induced Pluripotent Stem Cells Free of Vector and Transgene Sequences Science. 2009 Mar 26. pSIN4-EF2-IRES-Pur NANOG LIN28A CSDD1, LIN-28, LIN28, ZCCHC1, lin-28A Add to Cart
21164pSIN4-CMV-K2MKlf4, cMyc (Homo sapiens)Mammalian Expression, Lentiviral ; reprogrammingHuman Induced Pluripotent Stem Cells Free of Vector and Transgene Sequences Science. 2009 Mar 26. pSIN4-EF2-IRES-Pur MYC MRTL, MYCC, bHLHe39, c-Myc Add to Cart
21165pOTF3-AK (clone3)POUF5F1 (genomic DNA) (Homo sapiens)Cre/Lox ; homologous recombinationHomologous recombination in human embryonic stem cells. Nat Biotechnol. 2003 Mar . 21(3):319-21. pBluescript SK (-) POU5F1 OCT3, OCT4, OTF-3, OTF3, OTF4, Oct-3, Oct-4 Add to Cart
22884HPRThypoxanthine phosphoribosyltransferase-1 (Homo sapiens)Cre/Lox ; homologous recombinationHomologous recombination in human embryonic stem cells. Nat Biotechnol. 2003 Mar . 21(3):319-21. pBluescript SK (-) HPRT1 HGPRT, HPRT Add to Cart
30223pET-3c-rhVTN_FLrhVitronectin without deletions (Homo sapiens)Bacterial ExpressionChemically defined conditions for human iPSC derivation and culture. Nat Methods. 2011 Apr 10. ():. pET-3c VTN V75, VN, VNT Add to Cart
30224pET-3c-rhVTN-CrhVitronectin with C terminus cleavage (Homo sapiens)Bacterial ExpressionChemically defined conditions for human iPSC derivation and culture. Nat Methods. 2011 Apr 10. ():. pET-3c VTN V75, VN, VNT Add to Cart
30225pET-3c-rhVTN-NrhVitronectin with N terminus cleavage (Homo sapiens)Bacterial ExpressionChemically defined conditions for human iPSC derivation and culture. Nat Methods. 2011 Apr 10. ():. pET-3c VTN V75, VN, VNT Add to Cart
30226pET-3c-rhVTN-NCrhVitronectin with N and C terminus cleavages (Homo sapiens)Bacterial ExpressionChemically defined conditions for human iPSC derivation and culture. Nat Methods. 2011 Apr 10. ():. pET-3c VTN V75, VN, VNT Add to Cart
40629pCEP4_WT_OCT4POU5F1 (Homo sapiens)Mammalian ExpressionPhosphorylation regulates human OCT4. Proc Natl Acad Sci U S A. 2012 May 8;109(19):7162-8. Epub 2012 Apr 2. pCEP4 Add to Cart
40630pCEP4_OCT4_T234A_S235APOU5F1_S234A_T235A (Homo sapiens)Mammalian ExpressionPhosphorylation regulates human OCT4. Proc Natl Acad Sci U S A. 2012 May 8;109(19):7162-8. Epub 2012 Apr 2. pCEP4 POU5F1 OCT3, OCT4, OTF-3, OTF3, OTF4, Oct-3, Oct-4 Add to Cart
40631pCEP4_OCT4_T234E_S235EPOU5F1 (Homo sapiens)Mammalian ExpressionPhosphorylation regulates human OCT4. Proc Natl Acad Sci U S A. 2012 May 8;109(19):7162-8. Epub 2012 Apr 2. pCEP4 Add to Cart
40633pGEX4T-1_WT_OCT4POU5F1 (Homo sapiens)Bacterial ExpressionPhosphorylation regulates human OCT4. Proc Natl Acad Sci U S A. 2012 May 8;109(19):7162-8. Epub 2012 Apr 2. pGEX4T-1 Add to Cart
40634pGEX4T-1_OCT4_T234A_S235APOU5F1 (Homo sapiens)Mammalian ExpressionPhosphorylation regulates human OCT4. Proc Natl Acad Sci U S A. 2012 May 8;109(19):7162-8. Epub 2012 Apr 2. pCEP4
  • Tags / Fusion Proteins
    • GST (N terminal on insert)
    • Thrombin cleavage (N terminal on insert)
    • TEV cleavage (N terminal on insert)
  • Add to Cart
    40635pGEX4T-1_OCT4_T234E_S235EPOU5F1 (Homo sapiens)Bacterial ExpressionPhosphorylation regulates human OCT4. Proc Natl Acad Sci U S A. 2012 May 8;109(19):7162-8. Epub 2012 Apr 2. pGEX4T-1
  • Tags / Fusion Proteins
    • GST (N terminal on insert)
    • Thrombin Cleavage (N terminal on insert)
    • TEV Cleavage (N terminal on insert)
  • Add to Cart
    40636pGEX4T-1_OCT4_T234EPOU5F1 (Homo sapiens)Bacterial ExpressionPhosphorylation regulates human OCT4. Proc Natl Acad Sci U S A. 2012 May 8;109(19):7162-8. Epub 2012 Apr 2. pGEX4T-1
  • Tags / Fusion Proteins
    • GST (N terminal on insert)
    • Thrombin Cleavage (N terminal on insert)
    • TEV Cleavage (N terminal on insert)
  • Add to Cart
    40637pGEX4T-1_OCT4_T234APOU5F1 (Homo sapiens)Bacterial ExpressionPhosphorylation regulates human OCT4. Proc Natl Acad Sci U S A. 2012 May 8;109(19):7162-8. Epub 2012 Apr 2. pGEX4T-1 Add to Cart
    40638pGEX4T-1_OCT4_S235EPOU5F1 (Homo sapiens)Bacterial ExpressionPhosphorylation regulates human OCT4. Proc Natl Acad Sci U S A. 2012 May 8;109(19):7162-8. Epub 2012 Apr 2. pGEX4T-1
  • Tags / Fusion Proteins
    • GST (N terminal on insert)
    • Thrombin Cleavage (N terminal on insert)
    • TEV Cleavage (N terminal on insert)
  • Add to Cart
    40639pGEX4T-1_OCT4_S235APOU5F1 (Homo sapiens)Bacterial ExpressionPhosphorylation regulates human OCT4. Proc Natl Acad Sci U S A. 2012 May 8;109(19):7162-8. Epub 2012 Apr 2. pGEX4T-1
  • Tags / Fusion Proteins
    • GST (N terminal on insert)
    • Thrombin cleavage (N terminal on insert)
    • TEV cleavage (N terminal on insert)
  • Add to Cart
    47867pSimpleII-NLS-NmCas9-HA-NLS(s)NmCas9 (Other)Mammalian Expression, CRISPREfficient genome engineering in human pluripotent stem cells using Cas9 from Neisseria meningitidis. Proc Natl Acad Sci U S A. 2013 Aug 12. This plasmid encode NmCas9 with two NLS and a HA tag pSimpleII Add to Cart
    47868pSimpleII-U6-tracr-U6-BsmBI-NLS-NmCas9-HA-NLS(s)NmCas9 (Other), U6pr-tracrRNA (Other)Mammalian Expression, CRISPREfficient genome engineering in human pluripotent stem cells using Cas9 from Neisseria meningitidis. Proc Natl Acad Sci U S A. 2013 Aug 12. This plasmid contains expression cassette for NmCas9 with N and C NLS and an HA tag, a cassette for expression of tracrRNA, a cassette for cloning crRNA under the control of U6 promoter. pSimpleII Add to Cart
    47869pSimpleII-U6-tracr-U6-crRNA(tdTomato)-NLS-NmCas9-HA-NLS(s)NmCas9 (Other), U6pr-tracrRNA (Other), tdTomato crRNA (Synthetic)Mammalian Expression, CRISPREfficient genome engineering in human pluripotent stem cells using Cas9 from Neisseria meningitidis. Proc Natl Acad Sci U S A. 2013 Aug 12. All-in-one plasmid targeting tdTomato pSimpleII Add to Cart
    47870pSimpleII-U6-tracr-U6-crRNA(OCT4)-NLS-NmCas9-HA-NLS(s)NmCas9 (Other), U6pr-tracrRNA (Other), OCT4 crRNA (Synthetic)Mammalian Expression, CRISPREfficient genome engineering in human pluripotent stem cells using Cas9 from Neisseria meningitidis. Proc Natl Acad Sci U S A. 2013 Aug 12. All-in-one plasmid targeting human OCT4, cuts roughly ~84bp downstream of stop codon. pSimpleII Add to Cart
    47871pSTBlue-U6-sgRNA(tdTomato)sgRNA-tdTomato (Synthetic)Mammalian Expression, CRISPREfficient genome engineering in human pluripotent stem cells using Cas9 from Neisseria meningitidis. Proc Natl Acad Sci U S A. 2013 Aug 12. This plasmid contains expression cassette for sgRNA targeting tdTomato. pstBlue-1 Add to Cart
    47872pSimpleII-NmCas9-FLAGNmCas9 (Other)Mammalian Expression, CRISPREfficient genome engineering in human pluripotent stem cells using Cas9 from Neisseria meningitidis. Proc Natl Acad Sci U S A. 2013 Aug 12. FLAG tagged NmCas9 with no NLS. pSimpleII Add to Cart
    49157pSmart-Nm-sgRNA-BbsIMammalian Expression, CRISPR Thomson/Sontheimer Lab NmCas9 plasmids (unpublished) Plasmid for cloning spacer into sgRNA for NmCas9 pSmart-Kan-HC Add to Cart
    60665PB-Gata2Gata2 (Rattus norvegicus)Mammalian Expression ; PiggyBacAn expandable, inducible hemangioblast state regulated by fibroblast growth factor. Stem Cell Reports. 2014 Dec 9;3(6):1043-57. doi: 10.1016/j.stemcr.2014.10.003. Epub 2014 Nov 13. PiggyBac expression vector encoding rat Gata2 downstream of a doxycycline inducible promoter pBTO-Dest Gata2 Add to Cart
    60666PB-Lmo2Lmo2 (Mus musculus)Mammalian Expression ; PiggyBacAn expandable, inducible hemangioblast state regulated by fibroblast growth factor. Stem Cell Reports. 2014 Dec 9;3(6):1043-57. doi: 10.1016/j.stemcr.2014.10.003. Epub 2014 Nov 13. PiggyBac expression vector encoding murine Lmo2 downstream of a doxycycline inducible promoter pBTO-Dest Lmo2 Rbtn-2, Rbtn2, Rhom-2, Ttg2 Add to Cart
    60667PB-MycnMycn (Mus musculus)Mammalian Expression ; PiggyBacAn expandable, inducible hemangioblast state regulated by fibroblast growth factor. Stem Cell Reports. 2014 Dec 9;3(6):1043-57. doi: 10.1016/j.stemcr.2014.10.003. Epub 2014 Nov 13. PiggyBac expression vector encoding murine Mycn downstream of a doxycycline inducible promoter pBTO-Dest Mycn N-myc, Nmyc, Nmyc-1, Nmyc1, bHLHe37, c-nmyc Add to Cart
    60668PB-Pitx2Pitx2 (Mus musculus)Mammalian Expression ; PiggyBacAn expandable, inducible hemangioblast state regulated by fibroblast growth factor. Stem Cell Reports. 2014 Dec 9;3(6):1043-57. doi: 10.1016/j.stemcr.2014.10.003. Epub 2014 Nov 13. PiggyBac expression vector encoding murine Pitx2 downstream of a doxycycline inducible promoter pBTO-Dest Pitx2 9430085M16Rik, Brx1, Brx1b, Munc30, Otlx2, Ptx2, Rieg Add to Cart
    60669PB-Sox17Sox17 (Mus musculus)Mammalian Expression ; PiggyBacAn expandable, inducible hemangioblast state regulated by fibroblast growth factor. Stem Cell Reports. 2014 Dec 9;3(6):1043-57. doi: 10.1016/j.stemcr.2014.10.003. Epub 2014 Nov 13. PiggyBac expression vector encoding murine Sox17 downstream of a doxycycline inducible promoter pBTO-Dest Sox17 Add to Cart
    60670PB-Tal1Tal1 (Mus musculus)Mammalian Expression ; PiggyBacAn expandable, inducible hemangioblast state regulated by fibroblast growth factor. Stem Cell Reports. 2014 Dec 9;3(6):1043-57. doi: 10.1016/j.stemcr.2014.10.003. Epub 2014 Nov 13. PiggyBac expression vector encoding murine Tal1 downstream of a doxycycline inducible promoter pBTO-Dest Tal1 Hpt, SCL/tal-1, Scl, bHLHa17, tal-1 Add to Cart
    83344T-2A-EGFP-PGK-PuroHuman Brachyury knockin homology arms (Homo sapiens)Single-cell RNA-seq reveals novel regulators of human embryonic stem cell differentiation to definitive endoderm. Genome Biol. 2016 Aug 17;17(1):173. doi: 10.1186/s13059-016-1033-x. Donor vector to knocin EGFP into human Brachyury(T) locus Addgene plasmid: 31938 T SAVA, TFT Add to Cart
    83346T-STOP sgRNA-1T-STOP sgRNA (Homo sapiens)Mammalian ExpressionSingle-cell RNA-seq reveals novel regulators of human embryonic stem cell differentiation to definitive endoderm. Genome Biol. 2016 Aug 17;17(1):173. doi: 10.1186/s13059-016-1033-x. The sgRNA at the stop codon of human Brachyury(T) locus pCR-Blunt II-TOPO Add to Cart
    98748pSimple-miR302/367miR302/367 (miRNA cluster) (Homo sapiens)Mammalian ExpressionSimultaneous Reprogramming and Gene Correction of Patient Fibroblasts. Stem Cell Reports. 2015 Dec 8;5(6):1109-18. doi: 10.1016/j.stemcr.2015.10.009. Epub 2015 Nov 12. Episomal reprogramming pSimpleII Add to Cart