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Jen Sheen Lab Plasmids


The Jen Sheen Lab has deposited plasmids at Addgene for distribution to the research community. Addgene is a nonprofit plasmid repository dedicated to improving life science research.

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Title Authors Publication
Multiplex and homologous recombination-mediated genome editing in Arabidopsis and Nicotiana benthamiana using guide RNA and Cas9. Li JF, Norville JE, Aach J, McCormack M, Zhang D, Bush J, Church GM, Sheen J Nat Biotechnol. 2013 Aug;31(8):688-91. doi: 10.1038/nbt.2654.
Sheen lab CRISPR plasmids (unpublished)
Sheen lab CRISPR plasmids (unpublished)
ID Plasmid Gene/Insert Vector Type Publication Hidden Extra Search Info  
52254HBT-pcoCas9pcoCas9 (Synthetic)CRISPR ; plant expressionMultiplex and homologous recombination-mediated genome editing in Arabidopsis and Nicotiana benthamiana using guide RNA and Cas9. Nat Biotechnol. 2013 Aug;31(8):688-91. doi: 10.1038/nbt.2654. transient expression of pcoCas9 gene in plant cells HBT-FLAG Add to Cart
52255pUC119-gRNAguide RNA targeting AtPDS3 (Arabidopsis thaliana)CRISPR ; Plant expressionMultiplex and homologous recombination-mediated genome editing in Arabidopsis and Nicotiana benthamiana using guide RNA and Cas9. Nat Biotechnol. 2013 Aug;31(8):688-91. doi: 10.1038/nbt.2654. Can use a PCR template to assemble new desired guide RNA. Contains an Arabidopsis U6 promoter to drive guide RNA (targeting AtPDS3 gene target site 1) expression with a TTTTTT as terminator. pUC119 PDS3 AT4G14210, DL3145C, FCAALL.28, PDE226, PDS, PHYTOENE DESATURASE, PIGMENT DEFECTIVE 226, phytoene desaturase 3 Add to Cart
52256pFGC-pcoCas935SPPDK:pcoCas9:NOS cassette (Synthetic)CRISPR ; Plant expression Sheen lab CRISPR plasmids (unpublished) A binary plasmid containing 35SPPDK:pcoCas9:NOS cassette for plant expression of Cas9 and multiple cloning sites for insertion of guide RNA pFGC-RCS Add to Cart
58807pUC119-MCSPlant Expression, CRISPR Sheen lab CRISPR plasmids (unpublished) contains multiple cloning sites (MCSs) for cloning and transient expression of single or multiple guide RNA (gRNA) expression cassettes. pUC119 Add to Cart