Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at help@addgene.org. Learn more

Jon Ashwell Lab Plasmids


The Jon Ashwell Lab has deposited plasmids at Addgene for distribution to the research community. Addgene is a non-profit plasmid repository dedicated to improving life science research.

Learn more about research in the Jon Ashwell Lab.

Addgene Alerts

You must login or create an account to subscribe for Addgene Alerts.

Title Authors Publication
TNF-RII and c-IAP1 mediate ubiquitination and degradation of TRAF2. Li X, Yang Y, Ashwell JD Nature 2002 Mar 21;416(6878):345-7.
Ubiquitin protein ligase activity of IAPs and their degradation in proteasomes in response to apoptotic stimuli. Yang Y, Fang S, Jensen JP, Weissman AM, Ashwell JD Science 2000 May 5;288(5467):874-7.
Posttranscriptional downregulation of c-IAP2 by the ubiquitin protein ligase c-IAP1 in vivo. Conze DB, Albert L, Ferrick DA, Goeddel DV, Yeh WC, Mak T, Ashwell JD Mol Cell Biol. 2005 Apr . 25(8):3348-56.
NEMO is a sensor of Lys 63-linked polyubiquitination and functions in NF-kappaB activation. Wu CJ, Conze DB, Li T, Srinivasula SM, Ashwell JD Nat Cell Biol. 2006 Apr . 8(4):398-406.
NEMO recognition of ubiquitinated Bcl10 is required for T cell receptor-mediated NF-kappaB activation. Wu CJ, Ashwell JD Proc Natl Acad Sci U S A. 2008 Feb 26. 105(8):3023-8.
Optineurin negatively regulates TNFalpha- induced NF-kappaB activation by competing with NEMO for ubiquitinated RIP. Zhu G, Wu CJ, Zhao Y, Ashwell JD Curr Biol. 2007 Aug 21. 17(16):1438-43.
ID Plasmid Gene/Insert Vector Type Publication Hidden Extra Search Info  
8311pcdna3.1 hciap1cIAP1 (Homo sapiens)Mammalian ExpressionTNF-RII and c-IAP1 mediate ubiquitination and degradation of TRAF2. Nature 2002 Mar 21;416(6878):345-7. pcDNA3.1 BIRC2 API1, HIAP2, Hiap-2, MIHB, RNF48, c-IAP1, cIAP1 Add to Cart
8328pgex-cIAP1cIAP1 (Homo sapiens)Bacterial ExpressionTNF-RII and c-IAP1 mediate ubiquitination and degradation of TRAF2. Nature 2002 Mar 21;416(6878):345-7. pGEX BIRC2 API1, HIAP2, Hiap-2, MIHB, RNF48, c-IAP1, cIAP1 Add to Cart
8334pgex-cIAP1 mutciap1mut (Homo sapiens)Bacterial ExpressionTNF-RII and c-IAP1 mediate ubiquitination and degradation of TRAF2. Nature 2002 Mar 21;416(6878):345-7. pGEX BIRC2 API1, HIAP2, Hiap-2, MIHB, RNF48, c-IAP1, cIAP1 Add to Cart
8336pGEX-cIAP2mutciap2mut (Homo sapiens)Bacterial ExpressionTNF-RII and c-IAP1 mediate ubiquitination and degradation of TRAF2. Nature 2002 Mar 21;416(6878):345-7. pGEX BIRC3 AIP1, API2, CIAP2, HAIP1, HIAP1, MALT2, MIHC, RNF49, c-IAP2 Add to Cart
8337pcdna3.1 hciap1mutciap1mut (Homo sapiens)Mammalian ExpressionTNF-RII and c-IAP1 mediate ubiquitination and degradation of TRAF2. Nature 2002 Mar 21;416(6878):345-7. pcDNA3.1 BIRC2 API1, HIAP2, Hiap-2, MIHB, RNF48, c-IAP1, cIAP1 Add to Cart
8339pcdna3.1 hciap2mutciap2mut (Homo sapiens)Mammalian ExpressionTNF-RII and c-IAP1 mediate ubiquitination and degradation of TRAF2. Nature 2002 Mar 21;416(6878):345-7. pcDNA3.1 BIRC3 AIP1, API2, CIAP2, HAIP1, HIAP1, MALT2, MIHC, RNF49, c-IAP2 Add to Cart
8340pGEX-XIAPXIAP (Homo sapiens)Bacterial ExpressionUbiquitin protein ligase activity of IAPs and their degradation in proteasomes in response to apoptotic stimuli. Science 2000 May 5;288(5467):874-7. pGEX XIAP API3, BIRC4, IAP-3, ILP1, MIHA, XLP2, hIAP-3, hIAP3 Add to Cart
8341pGEX-XIAPmutXIAPmut (Homo sapiens)Bacterial ExpressionUbiquitin protein ligase activity of IAPs and their degradation in proteasomes in response to apoptotic stimuli. Science 2000 May 5;288(5467):874-7. pGEX XIAP API3, BIRC4, IAP-3, ILP1, MIHA, XLP2, hIAP-3, hIAP3 Add to Cart
8342pGEX-XIAP1-351XIAP1-351 (Homo sapiens)Bacterial ExpressionUbiquitin protein ligase activity of IAPs and their degradation in proteasomes in response to apoptotic stimuli. Science 2000 May 5;288(5467):874-7. pGEX XIAP API3, BIRC4, IAP-3, ILP1, MIHA, XLP2, hIAP-3, hIAP3 Add to Cart
11463pcmvtag2Bmciap2mciap2 (Mus musculus)Mammalian ExpressionPosttranscriptional downregulation of c-IAP2 by the ubiquitin protein ligase c-IAP1 in vivo. Mol Cell Biol. 2005 Apr . 25(8):3348-56. pcmvtag2B Birc3 AW107670, Api1, Api2, Birc2, HIAP2, IAP1, IAP2, MIAP1, MIAP2, MIHB, MIHC, RNF49, cIAP-1, cIAP-2, cIAP1, cIAP2 Add to Cart
11464pcmvtag2Bmciap2 mutmciap2mut (Mus musculus)Mammalian ExpressionPosttranscriptional downregulation of c-IAP2 by the ubiquitin protein ligase c-IAP1 in vivo. Mol Cell Biol. 2005 Apr . 25(8):3348-56. pcmvtag2B Birc3 AW107670, Api1, Api2, Birc2, HIAP2, IAP1, IAP2, MIAP1, MIAP2, MIHB, MIHC, RNF49, cIAP-1, cIAP-2, cIAP1, cIAP2 Add to Cart
11465pgex6p-1mciap1mciap1 (Mus musculus)Bacterial ExpressionPosttranscriptional downregulation of c-IAP2 by the ubiquitin protein ligase c-IAP1 in vivo. Mol Cell Biol. 2005 Apr . 25(8):3348-56. pGEX6p-1 Birc2 AW146227, Api1, Api2, Birc3, HIAP1, HIAP2, IAP1, IAP2, MIAP1, MIAP2, MIHB, MIHC, RNF48, cIAP1, cIAP2, mcIAP1 Add to Cart
11474pcdna3mciap1mciap1 (Mus musculus)Mammalian ExpressionPosttranscriptional downregulation of c-IAP2 by the ubiquitin protein ligase c-IAP1 in vivo. Mol Cell Biol. 2005 Apr . 25(8):3348-56. pcDNA3 Birc2 AW146227, Api1, Api2, Birc3, HIAP1, HIAP2, IAP1, IAP2, MIAP1, MIAP2, MIHB, MIHC, RNF48, cIAP1, cIAP2, mcIAP1 Add to Cart
11476pcdna3mciap1 mutmciap1mut (Mus musculus)Mammalian ExpressionPosttranscriptional downregulation of c-IAP2 by the ubiquitin protein ligase c-IAP1 in vivo. Mol Cell Biol. 2005 Apr . 25(8):3348-56. pcDNA3 Birc2 AW146227, Api1, Api2, Birc3, HIAP1, HIAP2, IAP1, IAP2, MIAP1, MIAP2, MIHB, MIHC, RNF48, cIAP1, cIAP2, mcIAP1 Add to Cart
11484pgex6p-1mciap1mutmciap1mut (Mus musculus)Bacterial ExpressionPosttranscriptional downregulation of c-IAP2 by the ubiquitin protein ligase c-IAP1 in vivo. Mol Cell Biol. 2005 Apr . 25(8):3348-56. pGEX6p-1 Birc2 AW146227, Api1, Api2, Birc3, HIAP1, HIAP2, IAP1, IAP2, MIAP1, MIAP2, MIHB, MIHC, RNF48, cIAP1, cIAP2, mcIAP1 Add to Cart
11558pEBB-XIAPXIAP (Homo sapiens)Mammalian ExpressionUbiquitin protein ligase activity of IAPs and their degradation in proteasomes in response to apoptotic stimuli. Science 2000 May 5;288(5467):874-7. pEBB XIAP API3, BIRC4, IAP-3, ILP1, MIHA, XLP2, hIAP-3, hIAP3 Add to Cart
11559pEBB-XIAPmutXIAPmut (Homo sapiens)Mammalian ExpressionUbiquitin protein ligase activity of IAPs and their degradation in proteasomes in response to apoptotic stimuli. Science 2000 May 5;288(5467):874-7. pEBB XIAP API3, BIRC4, IAP-3, ILP1, MIHA, XLP2, hIAP-3, hIAP3 Add to Cart
11560pEBB-XIAP1-351XIAP1-351 (Homo sapiens)Mammalian ExpressionUbiquitin protein ligase activity of IAPs and their degradation in proteasomes in response to apoptotic stimuli. Science 2000 May 5;288(5467):874-7. pEBB XIAP API3, BIRC4, IAP-3, ILP1, MIHA, XLP2, hIAP-3, hIAP3 Add to Cart
11965pGEX-NEMONEMO (Homo sapiens)Bacterial ExpressionNEMO is a sensor of Lys 63-linked polyubiquitination and functions in NF-kappaB activation. Nat Cell Biol. 2006 Apr . 8(4):398-406. pGEX4T1 IKBKG AMCBX1, FIP-3, FIP3, Fip3p, IKK-gamma, IKKAP1, IKKG, IMD33, IP, IP1, IP2, IPD2, NEMO, ZC2HC9 Add to Cart
11966pET-NEMONEMO (Homo sapiens)Bacterial ExpressionNEMO is a sensor of Lys 63-linked polyubiquitination and functions in NF-kappaB activation. Nat Cell Biol. 2006 Apr . 8(4):398-406. pET28a IKBKG AMCBX1, FIP-3, FIP3, Fip3p, IKK-gamma, IKKAP1, IKKG, IMD33, IP, IP1, IP2, IPD2, NEMO, ZC2HC9 Add to Cart
11967pGEX-NEMOL329PNEMOL329P (Homo sapiens)Bacterial ExpressionNEMO is a sensor of Lys 63-linked polyubiquitination and functions in NF-kappaB activation. Nat Cell Biol. 2006 Apr . 8(4):398-406. pGEX4T1 IKBKG AMCBX1, FIP-3, FIP3, Fip3p, IKK-gamma, IKKAP1, IKKG, IMD33, IP, IP1, IP2, IPD2, NEMO, ZC2HC9 Add to Cart
11968pGEX-NEMOD311NNEMOD311N (Homo sapiens)Bacterial ExpressionNEMO is a sensor of Lys 63-linked polyubiquitination and functions in NF-kappaB activation. Nat Cell Biol. 2006 Apr . 8(4):398-406. pGEX4T1 IKBKG AMCBX1, FIP-3, FIP3, Fip3p, IKK-gamma, IKKAP1, IKKG, IMD33, IP, IP1, IP2, IPD2, NEMO, ZC2HC9 Add to Cart
11969pET-NEMOL329PNEMOL329P (Homo sapiens)Bacterial ExpressionNEMO is a sensor of Lys 63-linked polyubiquitination and functions in NF-kappaB activation. Nat Cell Biol. 2006 Apr . 8(4):398-406. pET28a IKBKG AMCBX1, FIP-3, FIP3, Fip3p, IKK-gamma, IKKAP1, IKKG, IMD33, IP, IP1, IP2, IPD2, NEMO, ZC2HC9 Add to Cart
11970pCMVTAG-NEMONEMO (Homo sapiens)Mammalian ExpressionNEMO is a sensor of Lys 63-linked polyubiquitination and functions in NF-kappaB activation. Nat Cell Biol. 2006 Apr . 8(4):398-406. pCMVTAG2B IKBKG AMCBX1, FIP-3, FIP3, Fip3p, IKK-gamma, IKKAP1, IKKG, IMD33, IP, IP1, IP2, IPD2, NEMO, ZC2HC9 Add to Cart
11971pCMVTAG-NEMOL329PNEMOL329P (Homo sapiens)Mammalian ExpressionNEMO is a sensor of Lys 63-linked polyubiquitination and functions in NF-kappaB activation. Nat Cell Biol. 2006 Apr . 8(4):398-406. pCMVTAG2B IKBKG AMCBX1, FIP-3, FIP3, Fip3p, IKK-gamma, IKKAP1, IKKG, IMD33, IP, IP1, IP2, IPD2, NEMO, ZC2HC9 Add to Cart
11972pGEX-RIPRIP (Mus musculus)Bacterial ExpressionNEMO is a sensor of Lys 63-linked polyubiquitination and functions in NF-kappaB activation. Nat Cell Biol. 2006 Apr . 8(4):398-406. pGEX4T1 Ripk1 D330015H01Rik, RIP, Rinp, Rip1 Add to Cart
18718pMSCV-FlagBcl10B cell lymphoma 10 (Homo sapiens)Mammalian Expression, RetroviralNEMO recognition of ubiquitinated Bcl10 is required for T cell receptor-mediated NF-kappaB activation. Proc Natl Acad Sci U S A. 2008 Feb 26. 105(8):3023-8. pMSCVpuro BCL10 CARMEN, CIPER, CLAP, IMD37, c-E10, mE10 Add to Cart
18719pMSCV-FlagBcl10K31RK63RB cell lymphoma 10 (Homo sapiens)Mammalian Expression, RetroviralNEMO recognition of ubiquitinated Bcl10 is required for T cell receptor-mediated NF-kappaB activation. Proc Natl Acad Sci U S A. 2008 Feb 26. 105(8):3023-8. pMSCVpuro BCL10 CARMEN, CIPER, CLAP, IMD37, c-E10, mE10 Add to Cart
23050pDEST26-hOPTNOptineurin (Homo sapiens)Mammalian ExpressionOptineurin negatively regulates TNFalpha- induced NF-kappaB activation by competing with NEMO for ubiquitinated RIP. Curr Biol. 2007 Aug 21. 17(16):1438-43. pDEST26 OPTN ALS12, FIP2, GLC1E, HIP7, HYPL, NRP, TFIIIA-INTP Add to Cart
23051pDEST26-hOPTN-D474NOptineurin (Homo sapiens)Mammalian ExpressionOptineurin negatively regulates TNFalpha- induced NF-kappaB activation by competing with NEMO for ubiquitinated RIP. Curr Biol. 2007 Aug 21. 17(16):1438-43. pDEST26 OPTN ALS12, FIP2, GLC1E, HIP7, HYPL, NRP, TFIIIA-INTP Add to Cart
23052pDEST15-hOPTNoptineurin (Homo sapiens)Bacterial ExpressionOptineurin negatively regulates TNFalpha- induced NF-kappaB activation by competing with NEMO for ubiquitinated RIP. Curr Biol. 2007 Aug 21. 17(16):1438-43. pDEST15 OPTN ALS12, FIP2, GLC1E, HIP7, HYPL, NRP, TFIIIA-INTP Add to Cart
23053pDEST17-hOPTNoptineurin (Homo sapiens)Bacterial ExpressionOptineurin negatively regulates TNFalpha- induced NF-kappaB activation by competing with NEMO for ubiquitinated RIP. Curr Biol. 2007 Aug 21. 17(16):1438-43. pDEST17 OPTN ALS12, FIP2, GLC1E, HIP7, HYPL, NRP, TFIIIA-INTP Add to Cart
23054pDEST15-hOPTN-D474Noptineurin (Homo sapiens)Bacterial ExpressionOptineurin negatively regulates TNFalpha- induced NF-kappaB activation by competing with NEMO for ubiquitinated RIP. Curr Biol. 2007 Aug 21. 17(16):1438-43. pDEST15 OPTN ALS12, FIP2, GLC1E, HIP7, HYPL, NRP, TFIIIA-INTP Add to Cart
23055pDEST17-hOPTN-D474NOptineurin (Homo sapiens)Bacterial ExpressionOptineurin negatively regulates TNFalpha- induced NF-kappaB activation by competing with NEMO for ubiquitinated RIP. Curr Biol. 2007 Aug 21. 17(16):1438-43. pDEST17 OPTN ALS12, FIP2, GLC1E, HIP7, HYPL, NRP, TFIIIA-INTP Add to Cart