Jon Ashwell Lab Plasmids


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Title Authors Publication
NEMO recognition of ubiquitinated Bcl10 is required for T cell receptor-mediated NF-kappaB activation. Wu CJ, Ashwell JD Proc Natl Acad Sci U S A. 2008 Feb 26. 105(8):3023-8.
Optineurin negatively regulates TNFalpha- induced NF-kappaB activation by competing with NEMO for ubiquitinated RIP. Zhu G, Wu CJ, Zhao Y, Ashwell JD Curr Biol. 2007 Aug 21. 17(16):1438-43.
NEMO is a sensor of Lys 63-linked polyubiquitination and functions in NF-kappaB activation. Wu CJ, Conze DB, Li T, Srinivasula SM, Ashwell JD Nat Cell Biol. 2006 Apr . 8(4):398-406.
Posttranscriptional downregulation of c-IAP2 by the ubiquitin protein ligase c-IAP1 in vivo. Conze DB, Albert L, Ferrick DA, Goeddel DV, Yeh WC, Mak T, Ashwell JD Mol Cell Biol. 2005 Apr . 25(8):3348-56.
TNF-RII and c-IAP1 mediate ubiquitination and degradation of TRAF2. Li X, Yang Y, Ashwell JD Nature 2002 Mar 21;416(6878):345-7.
Ubiquitin protein ligase activity of IAPs and their degradation in proteasomes in response to apoptotic stimuli. Yang Y, Fang S, Jensen JP, Weissman AM, Ashwell JD Science 2000 May 5;288(5467):874-7.
ID Plasmid Gene/Insert Vector Type Publication Hidden Extra Search Info  
8311 pcdna3.1 hciap1 cIAP1 (Homo sapiens) Mammalian Expression Li et al Nature 2002 Mar 21;416(6878):345-7. pcDNA3.1 BIRC2 API1, HIAP2, Hiap-2, MIHB, RNF48, c-IAP1, cIAP1
8328 pgex-cIAP1 cIAP1 (Homo sapiens) Bacterial Expression Li et al Nature 2002 Mar 21;416(6878):345-7. pGEX BIRC2 API1, HIAP2, Hiap-2, MIHB, RNF48, c-IAP1, cIAP1
8334 pgex-cIAP1 mut ciap1mut (Homo sapiens) Bacterial Expression Li et al Nature 2002 Mar 21;416(6878):345-7. pGEX BIRC2 API1, HIAP2, Hiap-2, MIHB, RNF48, c-IAP1, cIAP1
8336 pGEX-cIAP2mut ciap2mut (Homo sapiens) Bacterial Expression Li et al Nature 2002 Mar 21;416(6878):345-7. pGEX BIRC3 AIP1, API2, CIAP2, HAIP1, HIAP1, MALT2, MIHC, RNF49, c-IAP2
8337 pcdna3.1 hciap1mut ciap1mut (Homo sapiens) Mammalian Expression Li et al Nature 2002 Mar 21;416(6878):345-7. pcDNA3.1 BIRC2 API1, HIAP2, Hiap-2, MIHB, RNF48, c-IAP1, cIAP1
8339 pcdna3.1 hciap2mut ciap2mut (Homo sapiens) Mammalian Expression Li et al Nature 2002 Mar 21;416(6878):345-7. pcDNA3.1 BIRC3 AIP1, API2, CIAP2, HAIP1, HIAP1, MALT2, MIHC, RNF49, c-IAP2
8340 pGEX-XIAP XIAP (Homo sapiens) Bacterial Expression Yang et al Science 2000 May 5;288(5467):874-7. pGEX XIAP RP1-315G1.5, API3, BIRC4, IAP-3, ILP1, MIHA, XLP2, hIAP-3, hIAP3
8341 pGEX-XIAPmut XIAPmut (Homo sapiens) Bacterial Expression Yang et al Science 2000 May 5;288(5467):874-7. pGEX XIAP RP1-315G1.5, API3, BIRC4, IAP-3, ILP1, MIHA, XLP2, hIAP-3, hIAP3
8342 pGEX-XIAP1-351 XIAP1-351 (Homo sapiens) Bacterial Expression Yang et al Science 2000 May 5;288(5467):874-7. pGEX XIAP RP1-315G1.5, API3, BIRC4, IAP-3, ILP1, MIHA, XLP2, hIAP-3, hIAP3
11463 pcmvtag2Bmciap2 mciap2 (Mus musculus) Mammalian Expression Conze et al Mol Cell Biol. 2005 Apr . 25(8):3348-56. pcmvtag2B Birc3 RP24-318P17.1, AW107670, Api1, Api2, Birc2, HIAP2, IAP1, IAP2, MIAP1, MIAP2, MIHB, MIHC, RNF49, cIAP-1, cIAP-2, cIAP1, cIAP2
11464 pcmvtag2Bmciap2 mut mciap2mut (Mus musculus) Mammalian Expression Conze et al Mol Cell Biol. 2005 Apr . 25(8):3348-56. pcmvtag2B Birc3 RP24-318P17.1, AW107670, Api1, Api2, Birc2, HIAP2, IAP1, IAP2, MIAP1, MIAP2, MIHB, MIHC, RNF49, cIAP-1, cIAP-2, cIAP1, cIAP2
11465 pgex6p-1mciap1 mciap1 (Mus musculus) Bacterial Expression Conze et al Mol Cell Biol. 2005 Apr . 25(8):3348-56. pGEX6p-1 Birc2 AW146227, Api1, Api2, Birc3, HIAP1, HIAP2, IAP1, IAP2, MIAP1, MIAP2, MIHB, MIHC, RNF48, cIAP1, cIAP2, mcIAP1
11474 pcdna3mciap1 mciap1 (Mus musculus) Mammalian Expression Conze et al Mol Cell Biol. 2005 Apr . 25(8):3348-56. pcDNA3 Birc2 AW146227, Api1, Api2, Birc3, HIAP1, HIAP2, IAP1, IAP2, MIAP1, MIAP2, MIHB, MIHC, RNF48, cIAP1, cIAP2, mcIAP1
11476 pcdna3mciap1 mut mciap1mut (Mus musculus) Mammalian Expression Conze et al Mol Cell Biol. 2005 Apr . 25(8):3348-56. pcDNA3 Birc2 AW146227, Api1, Api2, Birc3, HIAP1, HIAP2, IAP1, IAP2, MIAP1, MIAP2, MIHB, MIHC, RNF48, cIAP1, cIAP2, mcIAP1
11484 pgex6p-1mciap1mut mciap1mut (Mus musculus) Bacterial Expression Conze et al Mol Cell Biol. 2005 Apr . 25(8):3348-56. pGEX6p-1 Birc2 AW146227, Api1, Api2, Birc3, HIAP1, HIAP2, IAP1, IAP2, MIAP1, MIAP2, MIHB, MIHC, RNF48, cIAP1, cIAP2, mcIAP1
11558 pEBB-XIAP XIAP (Homo sapiens) Mammalian Expression Yang et al Science 2000 May 5;288(5467):874-7. pEBB XIAP RP1-315G1.5, API3, BIRC4, IAP-3, ILP1, MIHA, XLP2, hIAP-3, hIAP3
11559 pEBB-XIAPmut XIAPmut (Homo sapiens) Mammalian Expression Yang et al Science 2000 May 5;288(5467):874-7. pEBB XIAP RP1-315G1.5, API3, BIRC4, IAP-3, ILP1, MIHA, XLP2, hIAP-3, hIAP3
11560 pEBB-XIAP1-351 XIAP1-351 (Homo sapiens) Mammalian Expression Yang et al Science 2000 May 5;288(5467):874-7. pEBB XIAP RP1-315G1.5, API3, BIRC4, IAP-3, ILP1, MIHA, XLP2, hIAP-3, hIAP3
11965 pGEX-NEMO NEMO (Homo sapiens) Bacterial Expression Wu et al Nat Cell Biol. 2006 Apr . 8(4):398-406. pGEX4T1 IKBKG AMCBX1, FIP-3, FIP3, Fip3p, IKK-gamma, IMD33, IP, IP1, IP2, IPD2, NEMO, ZC2HC9
11966 pET-NEMO NEMO (Homo sapiens) Bacterial Expression Wu et al Nat Cell Biol. 2006 Apr . 8(4):398-406. pET28a IKBKG AMCBX1, FIP-3, FIP3, Fip3p, IKK-gamma, IMD33, IP, IP1, IP2, IPD2, NEMO, ZC2HC9
11967 pGEX-NEMOL329P NEMOL329P (Homo sapiens) Bacterial Expression Wu et al Nat Cell Biol. 2006 Apr . 8(4):398-406. pGEX4T1 IKBKG AMCBX1, FIP-3, FIP3, Fip3p, IKK-gamma, IMD33, IP, IP1, IP2, IPD2, NEMO, ZC2HC9
11968 pGEX-NEMOD311N NEMOD311N (Homo sapiens) Bacterial Expression Wu et al Nat Cell Biol. 2006 Apr . 8(4):398-406. pGEX4T1 IKBKG AMCBX1, FIP-3, FIP3, Fip3p, IKK-gamma, IMD33, IP, IP1, IP2, IPD2, NEMO, ZC2HC9
11969 pET-NEMOL329P NEMOL329P (Homo sapiens) Bacterial Expression Wu et al Nat Cell Biol. 2006 Apr . 8(4):398-406. pET28a IKBKG AMCBX1, FIP-3, FIP3, Fip3p, IKK-gamma, IMD33, IP, IP1, IP2, IPD2, NEMO, ZC2HC9
11970 pCMVTAG-NEMO NEMO (Homo sapiens) Mammalian Expression Wu et al Nat Cell Biol. 2006 Apr . 8(4):398-406. pCMVTAG2B IKBKG AMCBX1, FIP-3, FIP3, Fip3p, IKK-gamma, IMD33, IP, IP1, IP2, IPD2, NEMO, ZC2HC9
11971 pCMVTAG-NEMOL329P NEMOL329P (Homo sapiens) Mammalian Expression Wu et al Nat Cell Biol. 2006 Apr . 8(4):398-406. pCMVTAG2B IKBKG AMCBX1, FIP-3, FIP3, Fip3p, IKK-gamma, IMD33, IP, IP1, IP2, IPD2, NEMO, ZC2HC9
11972 pGEX-RIP RIP (Mus musculus) Bacterial Expression Wu et al Nat Cell Biol. 2006 Apr . 8(4):398-406. pGEX4T1 Ripk1 D330015H01Rik, RIP, Rinp, Rip1
18718 pMSCV-FlagBcl10 B cell lymphoma 10 (Homo sapiens) Mammalian Expression, Retroviral Wu et al Proc Natl Acad Sci U S A. 2008 Feb 26. 105(8):3023-8. pMSCVpuro BCL10 RP11-234D19.2, CARMEN, CIPER, CLAP, c-E10, mE10
18719 pMSCV-FlagBcl10K31RK63R B cell lymphoma 10 (Homo sapiens) Mammalian Expression, Retroviral Wu et al Proc Natl Acad Sci U S A. 2008 Feb 26. 105(8):3023-8. pMSCVpuro BCL10 RP11-234D19.2, CARMEN, CIPER, CLAP, c-E10, mE10
23050 pDEST26-hOPTN Optineurin (Homo sapiens) Mammalian Expression Zhu et al Curr Biol. 2007 Aug 21. 17(16):1438-43. pDEST26 OPTN RP11-730A19.1, ALS12, FIP2, GLC1E, HIP7, HYPL, NRP, TFIIIA-INTP
23051 pDEST26-hOPTN-D474N Optineurin (Homo sapiens) Mammalian Expression Zhu et al Curr Biol. 2007 Aug 21. 17(16):1438-43. pDEST26 OPTN RP11-730A19.1, ALS12, FIP2, GLC1E, HIP7, HYPL, NRP, TFIIIA-INTP
23052 pDEST15-hOPTN optineurin (Homo sapiens) Bacterial Expression Zhu et al Curr Biol. 2007 Aug 21. 17(16):1438-43. pDEST15 OPTN RP11-730A19.1, ALS12, FIP2, GLC1E, HIP7, HYPL, NRP, TFIIIA-INTP
23053 pDEST17-hOPTN optineurin (Homo sapiens) Bacterial Expression Zhu et al Curr Biol. 2007 Aug 21. 17(16):1438-43. pDEST17 OPTN RP11-730A19.1, ALS12, FIP2, GLC1E, HIP7, HYPL, NRP, TFIIIA-INTP
23054 pDEST15-hOPTN-D474N optineurin (Homo sapiens) Bacterial Expression Zhu et al Curr Biol. 2007 Aug 21. 17(16):1438-43. pDEST15 OPTN RP11-730A19.1, ALS12, FIP2, GLC1E, HIP7, HYPL, NRP, TFIIIA-INTP
23055 pDEST17-hOPTN-D474N Optineurin (Homo sapiens) Bacterial Expression Zhu et al Curr Biol. 2007 Aug 21. 17(16):1438-43. pDEST17 OPTN RP11-730A19.1, ALS12, FIP2, GLC1E, HIP7, HYPL, NRP, TFIIIA-INTP