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Klaus Foerstemann Lab Plasmids


The Klaus Foerstemann Lab has deposited plasmids at Addgene for distribution to the research community. Addgene is a nonprofit plasmid repository dedicated to improving life science research.

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Title Authors Publication
Efficient chromosomal gene modification with CRISPR/cas9 and PCR-based homologous recombination donors in cultured Drosophila cells. Bottcher R, Hollmann M, Merk K, Nitschko V, Obermaier C, Philippou-Massier J, Wieland I, Gaul U, Forstemann K Nucleic Acids Res. 2014 Apr 19.
A comprehensive toolbox for genome editing in cultured Drosophila cells Kunzelmann S, Böttcher R, Schmidts I, and Förstemann K. G3 (Bethesda). Published online April 13,2016
ID Plasmid Gene/Insert Vector Type Publication Hidden Extra Search Info  
52522pRB14S. pyogenes cas9 with humanized codon bias (Other)Insect ExpressionEfficient chromosomal gene modification with CRISPR/cas9 and PCR-based homologous recombination donors in cultured Drosophila cells. Nucleic Acids Res. 2014 Apr 19. expresses a myc-tagged version of hCas9 in Drosophila pCASPER5 Add to Cart
52527pRB17Drosophila U6C promoter (Drosophila melanogaster)Insect Expression, CRISPR ; blunt cloning kitEfficient chromosomal gene modification with CRISPR/cas9 and PCR-based homologous recombination donors in cultured Drosophila cells. Nucleic Acids Res. 2014 Apr 19. serves as template for PCR-mediated fusion of U6-promoter with sgRNA pJet1.2 Add to Cart
52528pMH3eGFP and Blasticigin resistance cassette (Other)blunt cloning kitEfficient chromosomal gene modification with CRISPR/cas9 and PCR-based homologous recombination donors in cultured Drosophila cells. Nucleic Acids Res. 2014 Apr 19. serves as PCR-template to generate HR donors for C-terminal GFP-tag pJet1.2 Add to Cart
52529pMH42x FLAG-tag (Synthetic)blunt cloning kitEfficient chromosomal gene modification with CRISPR/cas9 and PCR-based homologous recombination donors in cultured Drosophila cells. Nucleic Acids Res. 2014 Apr 19. serves as PCR-template to generate HR donors for C-terminal 2xFLAG-tag pJet1.2 Add to Cart
52530pIW1Twin-STREP-tag (Synthetic)blunt cloning kitEfficient chromosomal gene modification with CRISPR/cas9 and PCR-based homologous recombination donors in cultured Drosophila cells. Nucleic Acids Res. 2014 Apr 19. serves as PCR-template to generate HR donors for C-terminal Twin-Strep tag pJet1.2 Add to Cart
52531pMH5FLP recombinase (Saccharomyces cerevisiae)Insect ExpressionEfficient chromosomal gene modification with CRISPR/cas9 and PCR-based homologous recombination donors in cultured Drosophila cells. Nucleic Acids Res. 2014 Apr 19. expresses FLP recombinase in Drosophila pCASPER2 flp Add to Cart
52532pKF251Double-GFP (Saccharomyces cerevisiae)Insect ExpressionEfficient chromosomal gene modification with CRISPR/cas9 and PCR-based homologous recombination donors in cultured Drosophila cells. Nucleic Acids Res. 2014 Apr 19. Double-GFP reporter for DNA cleavage pCASPER2 Add to Cart
52533pKF257I-SceI (Saccharomyces cerevisiae)Insect ExpressionEfficient chromosomal gene modification with CRISPR/cas9 and PCR-based homologous recombination donors in cultured Drosophila cells. Nucleic Acids Res. 2014 Apr 19. expresses I-SceI nuclease in Drosophila pCASPER5 SCEI Q0160 Add to Cart
52559pRB20eYFP and Blasticigin resistance cassette (Synthetic)blunt cloning kitEfficient chromosomal gene modification with CRISPR/cas9 and PCR-based homologous recombination donors in cultured Drosophila cells. Nucleic Acids Res. 2014 Apr 19. serves as PCR-template to generate HR donors for C-terminal YFP-tag pJet1.2 Add to Cart
72851pSK23eGFP (Other)Insect ExpressionA comprehensive toolbox for genome editing in cultured Drosophila cells G3 (Bethesda). Published online April 13,2016 template for C-terminal eGFP tagging, Puromycin-resistance pJet1.2 Add to Cart
72852pSK24twin Strep tag (Other)Insect ExpressionA comprehensive toolbox for genome editing in cultured Drosophila cells G3 (Bethesda). Published online April 13,2016 template for C-terminal Twin-Strep tag, Puromycin-resistance pJet1.2 Add to Cart
72853pSK25double Flag (Other)Insect ExpressionA comprehensive toolbox for genome editing in cultured Drosophila cells G3 (Bethesda). Published online April 13,2016 template for C-terminal double-FLAG tag, Puromycin-resistance pJet1.2 Add to Cart
72854pSK32TEV-V5 tag (Other)Insect ExpressionA comprehensive toolbox for genome editing in cultured Drosophila cells G3 (Bethesda). Published online April 13,2016 template for C-terminal TEV-site + V5-tag, Puromycin-Resistance pJet1.2 Add to Cart
72855pSK15attP site (Other)Insect ExpressionA comprehensive toolbox for genome editing in cultured Drosophila cells G3 (Bethesda). Published online April 13,2016 template for insertion of attP site (C-terminal tag plasmid design), Blasticidin-Resistance pJet1.2 Add to Cart
72856pKF292base vector for Nterm-tag templates (Other)Insect ExpressionA comprehensive toolbox for genome editing in cultured Drosophila cells G3 (Bethesda). Published online April 13,2016 template base vector for N-terminal tagging, inducible Blasticidin resistance pJet1.2 Add to Cart
72857pKF2933xFlag tag (Other)Insect ExpressionA comprehensive toolbox for genome editing in cultured Drosophila cells G3 (Bethesda). Published online April 13,2016 template for N-terminal 3x FLAG tag, inducible Blasticidin resistance pJet1.2 Add to Cart
72858pKF294single Strep tag (Other)Insect ExpressionA comprehensive toolbox for genome editing in cultured Drosophila cells G3 (Bethesda). Published online April 13,2016 template for N-terminal 1x Strep tag, inducible Blasticidin resistance pJet1.2 Add to Cart
72859pKF290Strep - His8 - Strep -TEV tag (Other)Insect ExpressionA comprehensive toolbox for genome editing in cultured Drosophila cells G3 (Bethesda). Published online April 13,2016 template for N-terminal Strep-8xHis-Strep-TEV site tag, inducible Blasticidin resistance pJet1.2 Add to Cart
72860pRB30eGFP (Other)Insect ExpressionA comprehensive toolbox for genome editing in cultured Drosophila cells G3 (Bethesda). Published online April 13,2016 template for N-terminal eGFP tag, inducible Blasticidin resistance pJet1.2 Add to Cart
72861pRB36base vector for introduction of tags (Other)Insect ExpressionA comprehensive toolbox for genome editing in cultured Drosophila cells G3 (Bethesda). Published online April 13,2016 template base vector for N-terminal tagging, constitutive Blasticidin resistance pJet1.2 Add to Cart
72862pRB343xFlag tag (Other)Insect ExpressionA comprehensive toolbox for genome editing in cultured Drosophila cells G3 (Bethesda). Published online April 13,2016 template for N-terminal 3x FLAG tag, constitutive Blasticidin resistance pJet1.2 Add to Cart
72863pRB32single Strep tag (Other)Insect ExpressionA comprehensive toolbox for genome editing in cultured Drosophila cells G3 (Bethesda). Published online April 13,2016 template for N-terminal 1x Strep tag, constitutive Blasticidin resistance pJet1.2 Add to Cart
72864pRB38Strep - His8 - Strep -TEV tag (Other)Insect ExpressionA comprehensive toolbox for genome editing in cultured Drosophila cells G3 (Bethesda). Published online April 13,2016 template for N-terminal Strep-8xHis-Strep-TEV site tag, constitutive Blasticidin resistance pJet1.2 Add to Cart
72865pRB40eGFP (Other)Insect ExpressionA comprehensive toolbox for genome editing in cultured Drosophila cells G3 (Bethesda). Published online April 13,2016 template for N-terminal eGFP tag, constitutive Blasticidin resistance pJet1.2 Add to Cart
72866pRB35base vector for N-terminal tags, Puro (Synthetic)Insect ExpressionA comprehensive toolbox for genome editing in cultured Drosophila cells G3 (Bethesda). Published online April 13,2016 template base vector for N-terminal tagging, constitutive Puromycin resistance pJet1.2 Add to Cart
72867pRB333xFlag tag (Synthetic)Insect ExpressionA comprehensive toolbox for genome editing in cultured Drosophila cells G3 (Bethesda). Published online April 13,2016 template for N-terminal 3x FLAG tag, constitutive Puromycin resistance pJet1.2 Add to Cart
72868pRB31single Strep tag (Synthetic)Insect ExpressionA comprehensive toolbox for genome editing in cultured Drosophila cells G3 (Bethesda). Published online April 13,2016 template for N-terminal 1x Strep tag, constitutive Puromycin resistance pJet1.2 Add to Cart
72869pRB37Strep - His8 - Strep -TEV tag (Synthetic)Insect ExpressionA comprehensive toolbox for genome editing in cultured Drosophila cells G3 (Bethesda). Published online April 13,2016 template for N-terminal Strep-8xHis-Strep-TEV site tag, constitutive Puromycin resistance pJet1.2 Add to Cart
72870pRB39eGFP (Synthetic)Insect ExpressionA comprehensive toolbox for genome editing in cultured Drosophila cells G3 (Bethesda). Published online April 13,2016 template for N-terminal eGFP tag, constitutive Puromycin resistance pJet1.2 Add to Cart
72871pKF295FLP recombinase (Saccharomyces cerevisiae)Insect ExpressionA comprehensive toolbox for genome editing in cultured Drosophila cells G3 (Bethesda). Published online April 13,2016 expression of improved FLP recombinase pCASPER flp Add to Cart
74773pKF296TEV- 2xV5 tag (short version) (Other)Insect ExpressionA comprehensive toolbox for genome editing in cultured Drosophila cells G3 (Bethesda). Published online April 13,2016 template for C-terminal TEV-site + 2x V5-tag (short version), Blasticidin-Resistance pJet1.2 Add to Cart
74774pKF297TEV- 2xV5 tag (short version) (Other)Insect ExpressionA comprehensive toolbox for genome editing in cultured Drosophila cells G3 (Bethesda). Published online April 13,2016 template for C-terminal TEV-site + 2x V5-tag (short version), Puromycin-Resistance pJet1.2 Add to Cart
74886pSK41attP site (Other)Insect ExpressionA comprehensive toolbox for genome editing in cultured Drosophila cells G3 (Bethesda). Published online April 13,2016 template for insertion of attP site (C-terminal tag plasmid design), Puromycin-Resistance pJet1.2 Add to Cart
74887pIS1Act5C upstream homology region (Drosophila melanogaster), Act5C downstream homology (Drosophila melanogaster)Insect ExpressionA comprehensive toolbox for genome editing in cultured Drosophila cells G3 (Bethesda). Published online April 13,2016 targeting vector for C-terminal eGFP tag on Drosophila melanogaster Act5C pMH3 Add to Cart