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Kosuke Yusa Lab Plasmids


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Title Authors Publication
Genome-wide recessive genetic screening in mammalian cells with a lentiviral CRISPR-guide RNA library. Koike-Yusa H, Li Y, Tan EP, Velasco-Herrera Mdel C, Yusa K Nat Biotechnol. 2014 Mar;32(3):267-73. doi: 10.1038/nbt.2800. Epub 2013 Dec 23.
A CRISPR Dropout Screen Identifies Genetic Vulnerabilities and Therapeutic Targets in Acute Myeloid Leukemia. Tzelepis K, Koike-Yusa H, De Braekeleer E, Li Y, Metzakopian E, Dovey OM, Mupo A, Grinkevich V, Li M, Mazan M, Gozdecka M, Ohnishi S, Cooper J, Patel M, McKerrell T, Chen B, Domingues AF, Gallipoli P, Teichmann S, Ponstingl H, McDermott U, Saez-Rodriguez J, Huntly BJ, Iorio F, Pina C, Vassiliou GS, Yusa K Cell Rep. 2016 Oct 18;17(4):1193-1205. doi: 10.1016/j.celrep.2016.09.079.
ID Plasmid Gene/Insert Vector Type Publication Hidden Extra Search Info  
50946pKLV-U6gRNA(BbsI)-PGKpuro2ABFPNoneMammalian Expression, Lentiviral, CRISPRGenome-wide recessive genetic screening in mammalian cells with a lentiviral CRISPR-guide RNA library. Nat Biotechnol. 2014 Mar;32(3):267-73. doi: 10.1038/nbt.2800. Epub 2013 Dec 23. An empty gRNA expression vector pBluescript Add to Cart
67974pKLV2-U6gRNA5(BbsI)-PGKpuro2ABFP-WU6gRNA cassette, PGKpuro2ABFP cassette, WPRE (Homo sapiens)LentiviralA CRISPR Dropout Screen Identifies Genetic Vulnerabilities and Therapeutic Targets in Acute Myeloid Leukemia. Cell Rep. 2016 Oct 18;17(4):1193-1205. doi: 10.1016/j.celrep.2016.09.079. CRISPR gRNA expression vector with an improved scaffold and puro/BFP markers pKLV2 lentiviral vector Add to Cart
67975pKLV2-U6gRNA5(BbsI)-PGKpuro2AZsG-WU6gRNA cassette, PGKpuro2AZsG cassette, WPRE (Homo sapiens)LentiviralA CRISPR Dropout Screen Identifies Genetic Vulnerabilities and Therapeutic Targets in Acute Myeloid Leukemia. Cell Rep. 2016 Oct 18;17(4):1193-1205. doi: 10.1016/j.celrep.2016.09.079. CRISPR gRNA expression vector with an improved scaffold and puro/ZsG markers pKLV2 lentiviral vector Add to Cart
67976pKLV2-U6gRNA5(BbsI)-PGKpuro2AmAG-WU6gRNA cassette, PGKpuro2AmAG cassette, WPRE (Homo sapiens)LentiviralA CRISPR Dropout Screen Identifies Genetic Vulnerabilities and Therapeutic Targets in Acute Myeloid Leukemia. Cell Rep. 2016 Oct 18;17(4):1193-1205. doi: 10.1016/j.celrep.2016.09.079. CRISPR gRNA expression vector with an improved scaffold and puro/mAG markers pKLV2 lentiviral vector Add to Cart
67977pKLV2-U6gRNA5(BbsI)-PGKpuro2AmCherry-WU6gRNA cassette, PGKpuro2AmCherry cassette, WPRE (Homo sapiens)LentiviralA CRISPR Dropout Screen Identifies Genetic Vulnerabilities and Therapeutic Targets in Acute Myeloid Leukemia. Cell Rep. 2016 Oct 18;17(4):1193-1205. doi: 10.1016/j.celrep.2016.09.079. CRISPR gRNA expression vector with an improved scaffold and puro/mCherry markers pKLV2 lentiviral vector Add to Cart
67978pKLV2-EF1a-BsdCas9-WEF1a-Cas9 cassette, WPRE (Homo sapiens)Lentiviral, CRISPRA CRISPR Dropout Screen Identifies Genetic Vulnerabilities and Therapeutic Targets in Acute Myeloid Leukemia. Cell Rep. 2016 Oct 18;17(4):1193-1205. doi: 10.1016/j.celrep.2016.09.079. Lentiviral vector expressing Cas9 fused with the Blasticidin resistant gene at the N-terminus pKLV2 lentiviral vector Add to Cart
67979pKLV2-U6gRNA5(Empty)-PGKBFP2AGFP-WU6gRNA cassette, PGKBFP2AGFP cassette, WPRE (Homo sapiens)LentiviralA CRISPR Dropout Screen Identifies Genetic Vulnerabilities and Therapeutic Targets in Acute Myeloid Leukemia. Cell Rep. 2016 Oct 18;17(4):1193-1205. doi: 10.1016/j.celrep.2016.09.079. Cas9 activity reporter (control) with BFP and GFP. pKLV2 lentiviral vector Add to Cart
67980pKLV2-U6gRNA5(gGFP)-PGKBFP2AGFP-WU6gRNA cassette, PGKBFP2AGFP cassette, WPRE (Homo sapiens)LentiviralA CRISPR Dropout Screen Identifies Genetic Vulnerabilities and Therapeutic Targets in Acute Myeloid Leukemia. Cell Rep. 2016 Oct 18;17(4):1193-1205. doi: 10.1016/j.celrep.2016.09.079. Cas9 activity reporter with BFP and GFP. pKLV2 lentiviral vector Add to Cart
67981pKLV2-U6gRNA5(Empty)-PGKmCherry2AGFP-WU6gRNA cassette, PGKmCherry2AGFP cassette, WPRE (Homo sapiens)Lentiviral, CRISPRA CRISPR Dropout Screen Identifies Genetic Vulnerabilities and Therapeutic Targets in Acute Myeloid Leukemia. Cell Rep. 2016 Oct 18;17(4):1193-1205. doi: 10.1016/j.celrep.2016.09.079. Cas9 activity reporter (control) with mCherry and GFP pKLV2 lentiviral vector Add to Cart
67982pKLV2-U6gRNA5(gGFP)-PGKmCherry2AGFP-WU6gRNA cassette, PGKmCherry2AGFP cassette, WPRE (Homo sapiens)Lentiviral, CRISPRA CRISPR Dropout Screen Identifies Genetic Vulnerabilities and Therapeutic Targets in Acute Myeloid Leukemia. Cell Rep. 2016 Oct 18;17(4):1193-1205. doi: 10.1016/j.celrep.2016.09.079. Cas9 activity reporter with mCherry and GFP pKLV2 lentiviral vector Add to Cart
67983pKLV2-U6gRNA5(Empty)-PGKGFP2ABFP-WU6gRNA cassette, PGKGFP2ABFP cassette, WPRE (Homo sapiens)Lentiviral, CRISPRA CRISPR Dropout Screen Identifies Genetic Vulnerabilities and Therapeutic Targets in Acute Myeloid Leukemia. Cell Rep. 2016 Oct 18;17(4):1193-1205. doi: 10.1016/j.celrep.2016.09.079. Cas9 activity reporter (control) with GFP and BFP pKLV2 lentiviral vector Add to Cart
67984pKLV2-U6gRNA5(gBFP)-PGKGFP2ABFP-WU6gRNA cassette, PGKGFP2ABFP cassette, WPRE (Homo sapiens), Guide RNA targeting modified BFPLentiviral, CRISPRA CRISPR Dropout Screen Identifies Genetic Vulnerabilities and Therapeutic Targets in Acute Myeloid Leukemia. Cell Rep. 2016 Oct 18;17(4):1193-1205. doi: 10.1016/j.celrep.2016.09.079. Cas9 activity reporter with GFP and BFP pKLV2 lentiviral vector Add to Cart
67985pKLV2-U6gRNA5(Empty)-PGKmCherry2ABFP-WU6gRNA cassette, PGKmCherryABFP cassette, WPRE (Homo sapiens)Lentiviral, CRISPRA CRISPR Dropout Screen Identifies Genetic Vulnerabilities and Therapeutic Targets in Acute Myeloid Leukemia. Cell Rep. 2016 Oct 18;17(4):1193-1205. doi: 10.1016/j.celrep.2016.09.079. Cas9 activity reporter (control) with mCherry and BFP pKLV2 lentiviral vector Add to Cart
67986pKLV2-U6gRNA5(gBFP)-PGKmCherry2ABFP-WU6gRNA cassette, PGKmCherryABFP cassette, WPRE (Homo sapiens), Guide RNA targeting modified BFP (Synthetic)Lentiviral, CRISPRA CRISPR Dropout Screen Identifies Genetic Vulnerabilities and Therapeutic Targets in Acute Myeloid Leukemia. Cell Rep. 2016 Oct 18;17(4):1193-1205. doi: 10.1016/j.celrep.2016.09.079. Cas9 activity reporter with mCherry and BFP pKLV2 lentiviral vector Add to Cart
67987pRosa26-EF1a-hCas9IRESneoEF1a-hCas9IRESneopA (Synthetic)Mammalian ExpressionA CRISPR Dropout Screen Identifies Genetic Vulnerabilities and Therapeutic Targets in Acute Myeloid Leukemia. Cell Rep. 2016 Oct 18;17(4):1193-1205. doi: 10.1016/j.celrep.2016.09.079. Mouse Rosa26 targeting vector carrying the EF1a-hCas9-IRES-neo cassette pBluescriptII Add to Cart
67988Mouse genome-wide lentiviral CRISPR gRNA library version 2U6gRNA cassette, PGKpuro2ABFP cassette, WPRE (Homo sapiens), Mouse CRISPR guide RNA library v2 (Synthetic)Lentiviral, CRISPRA CRISPR Dropout Screen Identifies Genetic Vulnerabilities and Therapeutic Targets in Acute Myeloid Leukemia. Cell Rep. 2016 Oct 18;17(4):1193-1205. doi: 10.1016/j.celrep.2016.09.079. Mouse genome-wide CRISPR guide RNA library V2. (pKLV2-U6gRNA5(MouseV2)-PGKpuro2ABFP-W) pKLV2 lentiviral vector Add to Cart
67989Human genome-wide lentiviral CRISPR gRNA library version 1U6gRNA cassette, PGKpuro2ABFP cassette, WPRE (Homo sapiens), Human CRISPR guide RNA library v2 (Synthetic)Lentiviral, CRISPRA CRISPR Dropout Screen Identifies Genetic Vulnerabilities and Therapeutic Targets in Acute Myeloid Leukemia. Cell Rep. 2016 Oct 18;17(4):1193-1205. doi: 10.1016/j.celrep.2016.09.079. Human genome-wide CRISPR guide RNA library V1 (pKLV2-U6gRNA5(HumanV1)-PGKpuro2ABFP-W) pKLV2 lentiviral vector Add to Cart
67990pKLV2-U6gRNA3(BbsI)-PGKpuro2ABFPU6gRNA cassette with the conventional scaffold, PGKpuro2ABFP cassette (Homo sapiens)Lentiviral, CRISPRA CRISPR Dropout Screen Identifies Genetic Vulnerabilities and Therapeutic Targets in Acute Myeloid Leukemia. Cell Rep. 2016 Oct 18;17(4):1193-1205. doi: 10.1016/j.celrep.2016.09.079. CRISPR gRNA expression vector with the conventional scaffold and puro/BFP markers without WPRE pKLV2 lentiviral vector Add to Cart
67991pKLV2-U6gRNA5(BbsI)-PGKpuro2ABFPU6gRNA cassette with an improved scaffold, PGKpuro2ABFP cassette (Homo sapiens)Lentiviral, CRISPRA CRISPR Dropout Screen Identifies Genetic Vulnerabilities and Therapeutic Targets in Acute Myeloid Leukemia. Cell Rep. 2016 Oct 18;17(4):1193-1205. doi: 10.1016/j.celrep.2016.09.079. CRISPR gRNA expression vector with an improved l scaffold and puro/BFP markers without WPRE pKLV2 lentiviral vector Add to Cart
68343pKLV2-EF1a-Cas9Bsd-WEF1a-Cas9 cassette, WPRE (Homo sapiens)Lentiviral, CRISPRA CRISPR Dropout Screen Identifies Genetic Vulnerabilities and Therapeutic Targets in Acute Myeloid Leukemia. Cell Rep. 2016 Oct 18;17(4):1193-1205. doi: 10.1016/j.celrep.2016.09.079. Lentiviral vector expressing Cas9 fused with the Blasticidin resistant gene at the C-terminus pKLV2 lentiviral vector Add to Cart
72666pKLV2.2-h7SKgRNA5(SapI)-hU6gRNA5(BbsI)-PGKpuroBFP-Wh7SKgRNA cassette, hU6gRNA cassette, PGKpuroBFP cassette, WPRE (Synthetic)Mammalian Expression, Lentiviral, CRISPRA CRISPR Dropout Screen Identifies Genetic Vulnerabilities and Therapeutic Targets in Acute Myeloid Leukemia. Cell Rep. 2016 Oct 18;17(4):1193-1205. doi: 10.1016/j.celrep.2016.09.079. Lentiviral dual CRISPR gRNA expression vector (human 7SK and human U6 promoters) pKLV2 lentiviral vector Add to Cart
72667pKLV2.2-mU6gRNA5(SapI)-hU6gRNA5(BbsI)-PGKpuroBFP-WmU6gRNA cassette, hU6gRNA cassette, PGKpuroBFP cassette, WPRE (Synthetic)Mammalian Expression, Lentiviral, CRISPRA CRISPR Dropout Screen Identifies Genetic Vulnerabilities and Therapeutic Targets in Acute Myeloid Leukemia. Cell Rep. 2016 Oct 18;17(4):1193-1205. doi: 10.1016/j.celrep.2016.09.079. Lentiviral dual CRISPR gRNA expression vector (mouse U6 and human U6 promoters) pKLV2 lentiviral vector Add to Cart