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Linda Hicke Lab Plasmids


The Linda Hicke Lab has deposited plasmids at Addgene for distribution to the research community. Addgene is a non-profit plasmid repository dedicated to improving life science research.

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Title Authors Publication
The function of yeast epsin and Ede1 ubiquitin-binding domains during receptor internalization. Dores MR, Schnell JD, Maldonado-Baez L, Wendland B, Hicke L Traffic. 2010 Jan . 11(1):151-60.
Regulation of the RSP5 ubiquitin ligase by an intrinsic ubiquitin-binding site. French ME, Kretzmann BR, Hicke L J Biol Chem. 2009 May 1. 284(18):12071-9.
The Rsp5 ubiquitin ligase binds to and ubiquitinates members of the yeast CIN85-endophilin complex, Sla1-Rvs167. Stamenova SD, Dunn R, Adler AS, Hicke L J Biol Chem. 2004 Apr 16;279(16):16017-25. Epub 2004 Feb 3.
A ubiquitin-binding motif required for intramolecular monoubiquitylation, the CUE domain. Shih SC, Prag G, Francis SA, Sutanto MA, Hurley JH, Hicke L EMBO J. 2003 Mar 17;22(6):1273-81.
A function for monoubiquitination in the internalization of a G protein-coupled receptor. Terrell J, Shih S, Dunn R, Hicke L Mol Cell. 1998 Jan;1(2):193-202.
Linda Hicke lab plasmids (unpublished)
Linda Hicke lab unpublished Ubiquitin constructs (unpublished)
Linda Hicke Lab Plasmids (unpublished)
Plasmid Gene/Insert Vector Type Publication Hidden Extra Search Info  
YCplac22-ENT2 ENT2 (Saccharomyces cerevisiae) Yeast Expression The function of yeast epsin and Ede1 ubiquitin-binding domains during receptor internalization. Dores et al Traffic. 2010 Jan . 11(1):151-60. YCplac22 ENT2 YLR206W Add to Cart
pET-30 Ubwt (2) Ubiquitin (Saccharomyces cerevisiae) Bacterial Expression Regulation of the RSP5 ubiquitin ligase by an intrinsic ubiquitin-binding site. French et al J Biol Chem. 2009 May 1. 284(18):12071-9. pET-30 Xa/Lic Add to Cart
LHP306 - Ub - no Lys synthetic yeast Ubiquitin (Saccharomyces cerevisiae) Yeast Expression A function for monoubiquitination in the internalization of a G protein-coupled receptor. Terrell et al Mol Cell. 1998 Jan;1(2):193-202. pTER62 UBA1 YKL210W UBA2 YDR390C, UAL1 Add to Cart
LHP308 - Ub - wt synthetic yeast Ubiquitin (Saccharomyces cerevisiae) Yeast Expression A function for monoubiquitination in the internalization of a G protein-coupled receptor. Terrell et al Mol Cell. 1998 Jan;1(2):193-202. pES7 UBA1 YKL210W UBA2 YDR390C, UAL1 Add to Cart
LHP334-pcDNA3-STE2 STE2 (Saccharomyces cerevisiae) Mammalian Expression Linda Hicke Lab Plasmids (unpublished) pcDNA3 STE2 YFL026W Add to Cart
LHP416-PAN1-HA PAN1 (Saccharomyces cerevisiae) Yeast Expression Linda Hicke Lab Plasmids (unpublished) Unknown PAN1 YIR006C, DIM2, MDP3, MIP3 Add to Cart
LPH2781 - Ub-K63R synthetic yeast Ubiquitin (Saccharomyces cerevisiae) Yeast Expression Linda Hicke lab unpublished Ubiquitin constructs (unpublished) pES7 UBA1 YKL210W UBA2 YDR390C, UAL1 Add to Cart
LPH2782 - Ub-K48R synthetic yeast Ubiquitin (Saccharomyces cerevisiae) Yeast Expression Linda Hicke lab unpublished Ubiquitin constructs (unpublished) pES7 UBA1 YKL210W UBA2 YDR390C, UAL1 Add to Cart
LPH2776 - Ub-I44A synthetic yeast Ubiquitin (Saccharomyces cerevisiae) Yeast Expression Linda Hicke lab unpublished Ubiquitin constructs (unpublished) pES7 UBA1 YKL210W UBA2 YDR390C, UAL1 Add to Cart
LHP336 - HA-Ub-K48R synthetic yeast Ubiquitin (Saccharomyces cerevisiae) Yeast Expression Linda Hicke lab unpublished Ubiquitin constructs (unpublished) N/A UBA1 YKL210W UBA2 YDR390C, UAL1 Add to Cart
LHP327 - Ub-F45A/G76A synthetic yeast Ubiquitin (Saccharomyces cerevisiae) Yeast Expression Linda Hicke lab unpublished Ubiquitin constructs (unpublished) N/A UBA1 YKL210W UBA2 YDR390C, UAL1 Add to Cart
LHP809 - Ub-K63A-CYC1 synthetic yeast Ubiquitin (Saccharomyces cerevisiae) Yeast Expression Linda Hicke lab unpublished Ubiquitin constructs (unpublished) YEplac195 UBA1 YKL210W UBA2 YDR390C, UAL1 Add to Cart
LHP398 - Ub-K48R/K63R synthetic yeast Ubiquitin (Saccharomyces cerevisiae) Yeast Expression Linda Hicke lab unpublished Ubiquitin constructs (unpublished) N/A UBA1 YKL210W UBA2 YDR390C, UAL1 Add to Cart
LHP585 - Ub-CYC1 uba1 yeast (Saccharomyces cerevisiae) Yeast Expression A ubiquitin-binding motif required for intramolecular monoubiquitylation, the CUE domain. Shih et al EMBO J. 2003 Mar 17;22(6):1273-81. YEplac195 UBA1 YKL210W UBA2 YDR390C, UAL1 Add to Cart
LHP462 - Ub-HA synthetic yeast Ubiquitin (Saccharomyces cerevisiae) Yeast Expression Linda Hicke lab unpublished Ubiquitin constructs (unpublished) pES7 UBA1 YKL210W UBA2 YDR390C, UAL1 Add to Cart
LHP599 - pACT2-Ub synthetic yeast Ubiquitin (Saccharomyces cerevisiae) Yeast Expression Linda Hicke lab unpublished Ubiquitin constructs (unpublished) pACT2 AD UBA1 YKL210W UBA2 YDR390C, UAL1 Add to Cart
LHP424-YCplac33-STE2-WT STE2 (Saccharomyces cerevisiae) Yeast Expression Linda Hicke Lab Plasmids (unpublished) YCPlac33 STE2 YFL026W Add to Cart
LHP428-YCplac33-STE2-378Stop STE2 (Saccharomyces cerevisiae) Yeast Expression Linda Hicke Lab Plasmids (unpublished) YCPlac33 STE2 YFL026W Add to Cart
LHP905 - Gal1-Ub synthetic yeast Ubiquitin (Saccharomyces cerevisiae) Yeast Expression Linda Hicke lab unpublished Ubiquitin constructs (unpublished) N/A UBA1 YKL210W UBA2 YDR390C, UAL1 Add to Cart
LHP938 - Gal1-HA-Ub synthetic yeast Ubiquitin (Saccharomyces cerevisiae) Yeast Expression Linda Hicke lab unpublished Ubiquitin constructs (unpublished) pYES2 UBA1 YKL210W UBA2 YDR390C, UAL1 Add to Cart
LHP2690 - pET30-Ub-K48A synthetic yeast Ubiquitin (Saccharomyces cerevisiae) Bacterial Expression Linda Hicke lab unpublished Ubiquitin constructs (unpublished) pET-30 Xa/LIC UBA1 YKL210W UBA2 YDR390C, UAL1 Add to Cart
LHP2691 - pET30-Ub-K63A synthetic yeast Ubiquitin (Saccharomyces cerevisiae) Bacterial Expression Linda Hicke lab unpublished Ubiquitin constructs (unpublished) pET-30 Xa/LIC
  • Tag / Fusion Protein
    • HIS (N terminal on backbone)
  • UBA1 YKL210W UBA2 YDR390C, UAL1
    Add to Cart
    LHP2260 - HA-INP53 INP53 (Saccharomyces cerevisiae) Yeast Expression Linda Hicke lab plasmids (unpublished) YEplac181 INP53 YOR109W, SJL3, SOP2 Add to Cart
    LHP1481 - TOPO-ProteinA Protein A Cloning Linda Hicke lab plasmids (unpublished) TOPO Add to Cart
    LHP1480 - TOPO-TAP-tag TAP tag Cloning Linda Hicke lab plasmids (unpublished) TOPO Add to Cart
    LHP1472 - EDE1 EDE1 (Saccharomyces cerevisiae) Yeast Expression Linda Hicke lab plasmids (unpublished) YCplac33 EDE1 YBL047C, BUD15 Add to Cart
    LHP1468 - VPS23 VPS23 (Saccharomyces cerevisiae) Yeast Expression Linda Hicke lab plasmids (unpublished) pRS316 STP22 YCL008C, VPL15, VPS23 Add to Cart
    LHP1462 - INP51 INP51 (Saccharomyces cerevisiae) Yeast Expression Linda Hicke lab plasmids (unpublished) PCplac22 INP51 YIL002C, SJL1 Add to Cart
    LHP1427 - VPS8 VPS8 (Saccharomyces cerevisiae) Yeast Expression Linda Hicke lab plasmids (unpublished) pRS313 VPS8 YAL002W, FUN15, VPL8, VPT8 Add to Cart
    LHP879 - ENT2 ENT2 (Saccharomyces cerevisiae) Yeast Expression Linda Hicke lab plasmids (unpublished) YCplac22 ENT2 YLR206W Add to Cart
    LHP1263 - HA-VPS9 VPS9 (Saccharomyces cerevisiae) Yeast Expression A ubiquitin-binding motif required for intramolecular monoubiquitylation, the CUE domain. Shih et al EMBO J. 2003 Mar 17;22(6):1273-81. YCplac111 VPS9 YML097C, VPL31, VPT9 Add to Cart
    LHP1463 - INP52 INP52 (Saccharomyces cerevisiae) Yeast Expression The Rsp5 ubiquitin ligase binds to and ubiquitinates members of the yeast CIN85-endophilin complex, Sla1-Rvs167. Stamenova et al J Biol Chem. 2004 Apr 16;279(16):16017-25. Epub 2004 Feb 3. YCplac22 INP52 YNL106C, SJL2 Add to Cart
    LHP1049 - pFB-YCK2 YCK2 (Saccharomyces cerevisiae) Insect Expression Linda Hicke lab plasmids (unpublished) pFastbac YCK2 YNL154C Add to Cart
    LHP1048 - pFB-RSP5 RSP5 (Saccharomyces cerevisiae) Insect Expression Linda Hicke lab plasmids (unpublished) pFastbac RSP5 YER125W, MDP1, MUT2, NPI1, SMM1, UBY1 Add to Cart
    LHP1035 - ECFP-Cterm ECFP Cloning Linda Hicke lab plasmids (unpublished) pCR2.1 TOPO Add to Cart
    LHP1034 - ECFP-Nterm ECFP Cloning Linda Hicke lab plasmids (unpublished) pCR2.1 TOPO Add to Cart
    LHP1033 - EYFP-Cterm EYFP Cloning Linda Hicke lab plasmids (unpublished) pCR2.1 TOPO Add to Cart
    LHP1032 - EYFP-Nterm EYFP Cloning Linda Hicke lab plasmids (unpublished) pCR2.1 TOPO Add to Cart
    LHP1021 - BUL1 BUL1 (Saccharomyces cerevisiae) Yeast Expression Linda Hicke lab plasmids (unpublished) YCplac111 BUL1 YMR275C, DAG1, RDS1, SMM2 Add to Cart
    LHP556 - UBC4 UBC4 (Saccharomyces cerevisiae) Yeast Expression Linda Hicke lab plasmids (unpublished) YCplac22 UBC4 YBR082C Add to Cart