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Lionel Christiaen Lab Plasmids


The Lionel Christiaen Lab has deposited plasmids at Addgene for distribution to the research community. Addgene is a nonprofit plasmid repository dedicated to improving life science research.

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Title Authors Publication
Tissue-specific genome editing in Ciona embryos by CRISPR/Cas9. Stolfi A, Gandhi S, Salek F, Christiaen L Development. 2014 Nov;141(21):4115-20. doi: 10.1242/dev.114488.
Evaluation and rational design of guide RNAs for efficient CRISPR/Cas9-mediated mutagenesis in Ciona. Gandhi S, Haeussler M, Razy-Krajka F, Christiaen L, Stolfi A Dev Biol. 2017 May 1;425(1):8-20. doi: 10.1016/j.ydbio.2017.03.003. Epub 2017 Mar 22.
ID Plasmid Gene/Insert Vector Type Publication Hidden Extra Search Info  
59986U6>sgRNA(F+E)CRISPRTissue-specific genome editing in Ciona embryos by CRISPR/Cas9. Development. 2014 Nov;141(21):4115-20. doi: 10.1242/dev.114488. Empty vector for sgRNA cloning (F+E modified backbone) pCESAx Add to Cart
59987Eef1a-1955/-1>nls::Cas9::nlsnls::Cas9::nls (Other)CRISPRTissue-specific genome editing in Ciona embryos by CRISPR/Cas9. Development. 2014 Nov;141(21):4115-20. doi: 10.1242/dev.114488. Eef1a (EF1alpha) promoter driving nls::Cas9::nls pCESAx Add to Cart
59988Mesp-1916/-1>nls::Cas9::nlsnls::Cas9::nls (Other)CRISPRTissue-specific genome editing in Ciona embryos by CRISPR/Cas9. Development. 2014 Nov;141(21):4115-20. doi: 10.1242/dev.114488. Mesp driver driving nls::Cas9::nls pCESAx Add to Cart
59989Sox1/2/3-2373/+3>nls::Cas9::nlsnls::Cas9::nls (Other)CRISPRTissue-specific genome editing in Ciona embryos by CRISPR/Cas9. Development. 2014 Nov;141(21):4115-20. doi: 10.1242/dev.114488. Sox1/2/3 (SoxB1) driver driving nls::Cas9::nls pCESAx Add to Cart
59990U6>Ebf.774(F+E)Ebf.774 (F+E) sgRNACRISPRTissue-specific genome editing in Ciona embryos by CRISPR/Cas9. Development. 2014 Nov;141(21):4115-20. doi: 10.1242/dev.114488. U6 promoter driving Ebf.774 (F+E) sgRNA pCESAx Add to Cart
60006U6>Control(F+E)Control (F+E) sgRNACRISPRTissue-specific genome editing in Ciona embryos by CRISPR/Cas9. Development. 2014 Nov;141(21):4115-20. doi: 10.1242/dev.114488. U6 promoter driving control (F+E) sgRNA pCESAx Add to Cart
87542U6>XXEvaluation and rational design of guide RNAs for efficient CRISPR/Cas9-mediated mutagenesis in Ciona. Dev Biol. 2017 May 1;425(1):8-20. doi: 10.1016/j.ydbio.2017.03.003. Epub 2017 Mar 22. Component 1 of 2 for U6>sgRNA cassette OSO-PCR pcesa Add to Cart
87543XX>sgRNA F+EEvaluation and rational design of guide RNAs for efficient CRISPR/Cas9-mediated mutagenesis in Ciona. Dev Biol. 2017 May 1;425(1):8-20. doi: 10.1016/j.ydbio.2017.03.003. Epub 2017 Mar 22. Template 2 (of 2) for U6>sgRNA cassette OSO-PCR pcesa Add to Cart