Mark Howarth Lab Plasmids


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Title Authors Publication
SpyTag/SpyCatcher cyclization confers resilience to boiling on a mesophilic enzyme. Schoene C, Fierer JO, Bennett SP, Howarth M Angew Chem Int Ed Engl. 2014 Jun 10;53(24):6101-4. doi: 10.1002/anie.201402519. Epub 2014 May 9.
SpyLigase peptide-peptide ligation polymerizes affibodies to enhance magnetic cancer cell capture. Fierer JO, Veggiani G, Howarth M Proc Natl Acad Sci U S A. 2014 Mar 17.
Plug-And-Play Pairing Via Defined Divalent Streptavidins. Fairhead M, Krndija D, Lowe ED, Howarth M J Mol Biol. 2013 Sep 19. pii: S0022-2836(13)00600-1. doi: 10.1016/j.jmb.2013.09.016.
Peptide tag forming a rapid covalent bond to a protein, through engineering a bacterial adhesin. Zakeri B, Fierer JO, Celik E, Chittock EC, Schwarz-Linek U, Moy VT, Howarth M Proc Natl Acad Sci U S A. 2012 Mar 20;109(12):E690-7. Epub 2012 Feb 24.
A streptavidin variant with slower biotin dissociation and increased mechanostability. Chivers CE, Crozat E, Chu C, Moy VT, Sherratt DJ, Howarth M Nat Methods. 2010 Apr 11. ():.
Dead-dloop-Streptavidin-SpyCatcher (unpublished)
ID Plasmid Gene/Insert Vector Type Publication Hidden Extra Search Info  
24753 pET21a-Traptavidin Traptavidin Bacterial Expression Chivers et al Nat Methods. 2010 Apr 11. ():. pET21a
26054 pET21a-Core Traptavidin Core Traptavidin Bacterial Expression Chivers et al Nat Methods. 2010 Apr 11. ():. pET21a
35044 pDEST14-SpyCatcher SpyCatcher Bacterial Expression Zakeri et al Proc Natl Acad Sci U S A. 2012 Mar 20;109(12):E690-7. Epub 2012 Feb 24. pDEST14
35045 pDEST14-SpyCatcher EQ SpyCatcher EQ Bacterial Expression Zakeri et al Proc Natl Acad Sci U S A. 2012 Mar 20;109(12):E690-7. Epub 2012 Feb 24. pDEST14
35050 pET28a-SpyTagMBP SpyTagMBP Bacterial Expression Zakeri et al Proc Natl Acad Sci U S A. 2012 Mar 20;109(12):E690-7. Epub 2012 Feb 24. pET28a
46367 pET21-Streptavidin-Glutamate_Tag Streptavidin-Glutamate_Tag Bacterial Expression Fairhead et al J Mol Biol. 2013 Sep 19. pii: S0022-2836(13)00600-1. doi: 10.1016/j.jmb.2013.09.016. pET21
46368 pET21-Streptavidin-Dead Aspartate Loop Streptavidin-Dead Aspartate Loop Bacterial Expression Fairhead et al J Mol Biol. 2013 Sep 19. pii: S0022-2836(13)00600-1. doi: 10.1016/j.jmb.2013.09.016. pET21
51722 SpyLigase SpyLigase (Synthetic) Bacterial Expression Fierer et al Proc Natl Acad Sci U S A. 2014 Mar 17. Plasmid for recombinant expression in bacteria of SpyLigase, a domain that promotes the formation of an isopeptide bond between 2 peptide tags (SpyTag and KTag) pDEST14
51723 SUMO-KTag SUMO-KTag (Synthetic) Bacterial Expression Fierer et al Proc Natl Acad Sci U S A. 2014 Mar 17. Plasmid for recombinant expression in bacteria of SUMO domain fused to KTag, permitting covalent linkage to SpyTag pET28a
52656 SpyTag-β-Lactamase-SpyCatcher TEM-1 β-Lactamase (Synthetic) Bacterial Expression Schoene et al Angew Chem Int Ed Engl. 2014 Jun 10;53(24):6101-4. doi: 10.1002/anie.201402519. Epub 2014 May 9. Expresses SpyTag-SpyCatcher mediated cyclized version of TEM-1 β-lactamase (BLA). Cyclization of BLA led to an increase in thermal resilience of >60 degrees celsius. pET28a
59547 Dead Streptavidin-SpyCatcher Dead-dloop-Streptavidin-SpyCatcher (Other) Bacterial Expression Dead-dloop-Streptavidin-SpyCatcher (unpublished) pET21-DCatch encodes Dead streptavidin (negligible biotin binding) bearing a C-terminal SpyCatcher, for generation of chimeric SpyAvidin tetramers pET21a
59548 Dead Streptavidin-SpyTag Dead Streptavidin-SpyTag (Other) Bacterial Expression Dead-dloop-Streptavidin-SpyCatcher (unpublished) pET21-DTag encodes Dead streptavidin (negligible biotin binding) bearing a C-terminal SpyTag, for generation of chimeric SpyAvidin tetramers pET21a
59549 Traptavidin-E6 Core Traptavidin-E6 (Other) Bacterial Expression Dead-dloop-Streptavidin-SpyCatcher (unpublished) pET21-Tre for expression of traptavidin (high stability mutant of streptavidin) bearing a glutamic acid tag for ion-exchange chromatography pET21a