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Mark Howarth Lab Plasmids


The Mark Howarth Lab has deposited plasmids at Addgene for distribution to the research community. Addgene is a non-profit plasmid repository dedicated to improving life science research.

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Title Authors Publication
A streptavidin variant with slower biotin dissociation and increased mechanostability. Chivers CE, Crozat E, Chu C, Moy VT, Sherratt DJ, Howarth M Nat Methods. 2010 Apr 11. ():.
Peptide tag forming a rapid covalent bond to a protein, through engineering a bacterial adhesin. Zakeri B, Fierer JO, Celik E, Chittock EC, Schwarz-Linek U, Moy VT, Howarth M Proc Natl Acad Sci U S A. 2012 Mar 20;109(12):E690-7. Epub 2012 Feb 24.
Plug-And-Play Pairing Via Defined Divalent Streptavidins. Fairhead M, Krndija D, Lowe ED, Howarth M J Mol Biol. 2013 Sep 19. pii: S0022-2836(13)00600-1. doi: 10.1016/j.jmb.2013.09.016.
SpyLigase peptide-peptide ligation polymerizes affibodies to enhance magnetic cancer cell capture. Fierer JO, Veggiani G, Howarth M Proc Natl Acad Sci U S A. 2014 Mar 17.
SpyTag/SpyCatcher cyclization confers resilience to boiling on a mesophilic enzyme. Schoene C, Fierer JO, Bennett SP, Howarth M Angew Chem Int Ed Engl. 2014 Jun 10;53(24):6101-4. doi: 10.1002/anie.201402519. Epub 2014 May 9.
SpyAvidin hubs enable precise and ultrastable orthogonal nanoassembly. Fairhead M, Veggiani G, Lever M, Yan J, Mesner D, Robinson CV, Dushek O, van der Merwe PA, Howarth M J Am Chem Soc. 2014 Sep 3;136(35):12355-63. doi: 10.1021/ja505584f. Epub 2014 Aug 21.
Programmable polyproteams built using twin peptide superglues. Veggiani G, Nakamura T, Brenner MD, Gayet RV, Yan J, Robinson CV, Howarth M Proc Natl Acad Sci U S A. 2016 Jan 19. pii: 201519214.
ID Plasmid Gene/Insert Vector Type Publication Hidden Extra Search Info  
24753pET21a-TraptavidinTraptavidinBacterial ExpressionA streptavidin variant with slower biotin dissociation and increased mechanostability. Nat Methods. 2010 Apr 11. ():. pET21a Add to Cart
26054pET21a-Core TraptavidinCore TraptavidinBacterial ExpressionA streptavidin variant with slower biotin dissociation and increased mechanostability. Nat Methods. 2010 Apr 11. ():. pET21a Add to Cart
35044pDEST14-SpyCatcherSpyCatcherBacterial ExpressionPeptide tag forming a rapid covalent bond to a protein, through engineering a bacterial adhesin. Proc Natl Acad Sci U S A. 2012 Mar 20;109(12):E690-7. Epub 2012 Feb 24. pDEST14 Add to Cart
35045pDEST14-SpyCatcher EQSpyCatcher EQBacterial ExpressionPeptide tag forming a rapid covalent bond to a protein, through engineering a bacterial adhesin. Proc Natl Acad Sci U S A. 2012 Mar 20;109(12):E690-7. Epub 2012 Feb 24. pDEST14 Add to Cart
35050pET28a-SpyTagMBPSpyTagMBPBacterial ExpressionPeptide tag forming a rapid covalent bond to a protein, through engineering a bacterial adhesin. Proc Natl Acad Sci U S A. 2012 Mar 20;109(12):E690-7. Epub 2012 Feb 24. pET28a Add to Cart
46367pET21-Streptavidin-Glutamate_TagStreptavidin-Glutamate_TagBacterial ExpressionPlug-And-Play Pairing Via Defined Divalent Streptavidins. J Mol Biol. 2013 Sep 19. pii: S0022-2836(13)00600-1. doi: 10.1016/j.jmb.2013.09.016. pET21 Add to Cart
46368pET21-Streptavidin-Dead Aspartate LoopStreptavidin-Dead Aspartate LoopBacterial ExpressionPlug-And-Play Pairing Via Defined Divalent Streptavidins. J Mol Biol. 2013 Sep 19. pii: S0022-2836(13)00600-1. doi: 10.1016/j.jmb.2013.09.016. pET21 Add to Cart
51722SpyLigaseSpyLigase (Synthetic)Bacterial ExpressionSpyLigase peptide-peptide ligation polymerizes affibodies to enhance magnetic cancer cell capture. Proc Natl Acad Sci U S A. 2014 Mar 17. Plasmid for recombinant expression in bacteria of SpyLigase, a domain that promotes the formation of an isopeptide bond between 2 peptide tags (SpyTag and KTag) pDEST14 Add to Cart
51723SUMO-KTagSUMO-KTag (Synthetic)Bacterial ExpressionSpyLigase peptide-peptide ligation polymerizes affibodies to enhance magnetic cancer cell capture. Proc Natl Acad Sci U S A. 2014 Mar 17. Plasmid for recombinant expression in bacteria of SUMO domain fused to KTag, permitting covalent linkage to SpyTag pET28a Add to Cart
52656SpyTag-β-Lactamase-SpyCatcherTEM-1 β-Lactamase (Synthetic)Bacterial ExpressionSpyTag/SpyCatcher cyclization confers resilience to boiling on a mesophilic enzyme. Angew Chem Int Ed Engl. 2014 Jun 10;53(24):6101-4. doi: 10.1002/anie.201402519. Epub 2014 May 9. Expresses SpyTag-SpyCatcher mediated cyclized version of TEM-1 β-lactamase (BLA). Cyclization of BLA led to an increase in thermal resilience of >60 degrees celsius. pET28a Add to Cart
59547Dead Streptavidin-SpyCatcherDead-dloop-Streptavidin-SpyCatcher (Other)Bacterial ExpressionSpyAvidin hubs enable precise and ultrastable orthogonal nanoassembly. J Am Chem Soc. 2014 Sep 3;136(35):12355-63. doi: 10.1021/ja505584f. Epub 2014 Aug 21. pET21-DCatch encodes Dead streptavidin (negligible biotin binding) bearing a C-terminal SpyCatcher, for generation of chimeric SpyAvidin tetramers pET21a Add to Cart
59548Dead Streptavidin-SpyTagDead Streptavidin-SpyTag (Other)Bacterial ExpressionSpyAvidin hubs enable precise and ultrastable orthogonal nanoassembly. J Am Chem Soc. 2014 Sep 3;136(35):12355-63. doi: 10.1021/ja505584f. Epub 2014 Aug 21. pET21-DTag encodes Dead streptavidin (negligible biotin binding) bearing a C-terminal SpyTag, for generation of chimeric SpyAvidin tetramers pET21a Add to Cart
59549Traptavidin-E6Core Traptavidin-E6 (Other)Bacterial ExpressionSpyAvidin hubs enable precise and ultrastable orthogonal nanoassembly. J Am Chem Soc. 2014 Sep 3;136(35):12355-63. doi: 10.1021/ja505584f. Epub 2014 Aug 21. pET21-Tre for expression of traptavidin (high stability mutant of streptavidin) bearing a glutamic acid tag for ion-exchange chromatography pET21a Add to Cart
72322pET28a-SnoopCatcherpET28a-SnoopCatcher (Synthetic)Bacterial ExpressionProgrammable polyproteams built using twin peptide superglues. Proc Natl Acad Sci U S A. 2016 Jan 19. pii: 201519214. Bacterial expression of SnoopCatcher, an engineered protein forming a spontaneous covalent bond with its cognate peptide partner SnoopTag. pET28a Add to Cart
72323pET28a SnoopTag-MBPpET28a SnoopTag-MBP (Synthetic)Bacterial ExpressionProgrammable polyproteams built using twin peptide superglues. Proc Natl Acad Sci U S A. 2016 Jan 19. pii: 201519214. Bacterial expression of Maltose Binding Protein linked to SnoopTag, a peptide tag forming a spontaneous covalent bond with its protein partner Snoopcatcher. pET28a Add to Cart
72324pET28a SpyCatcher-SnoopCatcherpET28a SpyCatcher-SnoopCatcher (Synthetic)Bacterial ExpressionProgrammable polyproteams built using twin peptide superglues. Proc Natl Acad Sci U S A. 2016 Jan 19. pii: 201519214. Bacterial expression of SpyCatcher linked to SnoopCatcher. pET21b Add to Cart
72325pET28a SnoopTag-mEGFP-SpyTagpET28a SnoopTag-mEGFP-SpyTag (Synthetic)Bacterial ExpressionProgrammable polyproteams built using twin peptide superglues. Proc Natl Acad Sci U S A. 2016 Jan 19. pii: 201519214. Bacterial expression of monomeric enhanced green fluorescent protein (mEGFP) containing SnoopTag at the N-terminus and SpyTag at the C-terminus, for programmed polyprotein synthesis. pET28a Add to Cart
72326AviTag-SpyCatcherAviTag-SpyCatcher (Synthetic)Bacterial ExpressionProgrammable polyproteams built using twin peptide superglues. Proc Natl Acad Sci U S A. 2016 Jan 19. pii: 201519214. Bacterial expression of SpyCatcher with a peptide tag enabling site-specific biotinylation. pDEST14-SpyCatcher Add to Cart
72327pET21 MBPx-SpyCatcherpET21 MBPx-SpyCatcher (Synthetic)Bacterial ExpressionProgrammable polyproteams built using twin peptide superglues. Proc Natl Acad Sci U S A. 2016 Jan 19. pii: 201519214. Bacterial expression of tandem high affinity maltose binding protein domains linked to SpyCatcher, for solid-phase polyprotein synthesis. pET21b Add to Cart