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Martin Dorf Lab Plasmids


The Martin Dorf Lab has deposited plasmids at Addgene for distribution to the research community. Addgene is a nonprofit plasmid repository dedicated to improving life science research.

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Title Authors Publication
RNAi screen in mouse astrocytes identifies phosphatases that regulate NF-kappaB signaling. Li S, Wang L, Berman MA, Zhang Y, Dorf ME Mol Cell. 2006 Nov 17. 24(4):497-509.
PKC phosphorylation of TRAF2 mediates IKKalpha/beta recruitment and K63-linked polyubiquitination. Li S, Wang L, Dorf ME Mol Cell. 2009 Jan 16. 33(1):30-42.
Mapping a dynamic innate immunity protein interaction network regulating type I interferon production. Li S, Wang L, Berman M, Kong YY, Dorf ME Immunity. 2011 Sep 23;35(3):426-40. Epub 2011 Sep 8.
The ubiquitin conjugating enzyme UBE2L3 regulates TNFalpha-induced linear ubiquitination. Fu B, Li S, Wang L, Berman MA, Dorf ME Cell Res. 2013 Sep 24. doi: 10.1038/cr.2013.133.
TRIM65 regulates microRNA activity by ubiquitination of TNRC6. Li S, Wang L, Fu B, Berman MA, Diallo A, Dorf ME Proc Natl Acad Sci U S A. 2014 May 13;111(19):6970-5. doi: 10.1073/pnas.1322545111. Epub 2014 Apr 28.
TRIM32 Senses and Restricts Influenza A Virus by Ubiquitination of PB1 Polymerase. Fu B, Wang L, Ding H, Schwamborn JC, Li S, Dorf ME PLoS Pathog. 2015 Jun 9;11(6):e1004960. doi: 10.1371/journal.ppat.1004960. eCollection 2015 Jun.
ZMPSTE24 defends against influenza and other pathogenic viruses. Fu B, Wang L, Li S, Dorf ME J Exp Med. 2017 Feb 28. pii: jem.20161270. doi: 10.1084/jem.20161270.
ID Plasmid Gene/Insert Vector Type Publication Hidden Extra Search Info  
14055pBabe-DualU6 and H1 promotersMammalian Expression, Retroviral, RNAiRNAi screen in mouse astrocytes identifies phosphatases that regulate NF-kappaB signaling. Mol Cell. 2006 Nov 17. 24(4):497-509. pBabe Add to Cart
20229hTRAF2pLPCX-HA-Flag/P874TNF receptor-associated factor 2 (Homo sapiens)Mammalian Expression, RetroviralPKC phosphorylation of TRAF2 mediates IKKalpha/beta recruitment and K63-linked polyubiquitination. Mol Cell. 2009 Jan 16. 33(1):30-42. pLPCX TRAF2 MGC:45012, TRAP, TRAP3 Add to Cart
20230hTRAF2T117ApLPCX-HA-Flag/P875TNF receptor-associated factor 2 (Homo sapiens)Mammalian Expression, RetroviralPKC phosphorylation of TRAF2 mediates IKKalpha/beta recruitment and K63-linked polyubiquitination. Mol Cell. 2009 Jan 16. 33(1):30-42. pLPCX TRAF2 MGC:45012, TRAP, TRAP3 Add to Cart
20231hTRAF2K31RpLPCX-HA-Flag/P876TNF receptor-associated factor 2 (Homo sapiens)Mammalian Expression, RetroviralPKC phosphorylation of TRAF2 mediates IKKalpha/beta recruitment and K63-linked polyubiquitination. Mol Cell. 2009 Jan 16. 33(1):30-42. pLPCX TRAF2 MGC:45012, TRAP, TRAP3 Add to Cart
37116mMib1-FLAGMib1(Mus musculus mindbomb homolog 1 (Drosophila) (Mib1)) (Mus musculus)Mammalian ExpressionMapping a dynamic innate immunity protein interaction network regulating type I interferon production. Immunity. 2011 Sep 23;35(3):426-40. Epub 2011 Sep 8. pCMV-Tag4a Mib1 DIP-1, E430019M12Rik, Mib, mKIAA1323 Add to Cart
37117MIB2-FLAGMIB2, mindbomb E3 ubiquitin protein ligase 2 (Homo sapiens)Mammalian ExpressionMapping a dynamic innate immunity protein interaction network regulating type I interferon production. Immunity. 2011 Sep 23;35(3):426-40. Epub 2011 Sep 8. pCMV-3Tag-8 MIB2 RP11-345P4.1, FLJ20648, FLJ39787, FLJ41406, ZZANK1, ZZZ5 Add to Cart
50014pCMV3flag8SHARPINSHANK-associated RH domain interactor (Homo sapiens)Mammalian ExpressionThe ubiquitin conjugating enzyme UBE2L3 regulates TNFalpha-induced linear ubiquitination. Cell Res. 2013 Sep 24. doi: 10.1038/cr.2013.133. Mammalian expression of human SHARPIN with a c-terminal 3xFLAG tag, and hygromycin selection. pCMV-3Tag-8 SHARPIN SIPL1 Add to Cart
50015pCMV3flag8HOIPring finger protein 31 (Homo sapiens)Mammalian ExpressionThe ubiquitin conjugating enzyme UBE2L3 regulates TNFalpha-induced linear ubiquitination. Cell Res. 2013 Sep 24. doi: 10.1038/cr.2013.133. Mammalian expression of human HOIP (RNF31) with a c-terminal 3xFLAG tag, and hygromycin selection. pCMV-3Tag-8 Add to Cart
50016pCMV3flag8HOIL-1LRanBP-type and C3HC4-type zinc finger containing 1 (Homo sapiens)Mammalian ExpressionThe ubiquitin conjugating enzyme UBE2L3 regulates TNFalpha-induced linear ubiquitination. Cell Res. 2013 Sep 24. doi: 10.1038/cr.2013.133. Mammalian expression of human HOIL-1L (RBCK1) with a c-terminal 3xFLAG tag, and hygromycin selection. pCMV-3Tag-8 Add to Cart
53966pCMVhTRIM65_3FlagTRIM65 (Homo sapiens)Mammalian ExpressionTRIM65 regulates microRNA activity by ubiquitination of TNRC6. Proc Natl Acad Sci U S A. 2014 May 13;111(19):6970-5. doi: 10.1073/pnas.1322545111. Epub 2014 Apr 28. Human TRIM65 mammalian expression vector with 3xFLAG epitope at c-terminus. pCMV-3Tag-8 TRIM65 4732463G12Rik Add to Cart
53968pCMVhPOP1_3FlagPOP1 (Homo sapiens)Mammalian ExpressionTRIM65 regulates microRNA activity by ubiquitination of TNRC6. Proc Natl Acad Sci U S A. 2014 May 13;111(19):6970-5. doi: 10.1073/pnas.1322545111. Epub 2014 Apr 28. Mammalian expression vector for human POP1 with c-terminal 3xFLAG epitopes. pCMV-3Tag-8 POP1 ANXD2 Add to Cart
53974pCMV3FLAG_hGTPBP4GTPBP4 (Homo sapiens)Mammalian ExpressionTRIM65 regulates microRNA activity by ubiquitination of TNRC6. Proc Natl Acad Sci U S A. 2014 May 13;111(19):6970-5. doi: 10.1073/pnas.1322545111. Epub 2014 Apr 28. Mammalian expression plasmid for human GTPBP4 with n-terminal 3xFLAG epitope fused. pCMV-3Tag-6 GTPBP4 RP11-38M7.5, CRFG, NGB, NOG1 Add to Cart
54320hTRIM65_EGFP_N1TRIM65 (Homo sapiens)Mammalian ExpressionTRIM65 regulates microRNA activity by ubiquitination of TNRC6. Proc Natl Acad Sci U S A. 2014 May 13;111(19):6970-5. doi: 10.1073/pnas.1322545111. Epub 2014 Apr 28. Mammalian expression vector for human TRIM65 fusion protein with c-terminal EGFP. pEGFP-N1 TRIM65 4732463G12Rik Add to Cart
69541pEGFP-N1_hTRIM32tripartite motif containing 32 (Homo sapiens)Mammalian ExpressionTRIM32 Senses and Restricts Influenza A Virus by Ubiquitination of PB1 Polymerase. PLoS Pathog. 2015 Jun 9;11(6):e1004960. doi: 10.1371/journal.ppat.1004960. eCollection 2015 Jun. Expresses human TRIM32 fused to c-terminal EGFP pEGFP-N1 Add to Cart
89759hZMPSTE24-3FLAG_pQCXIPHomo sapiens zinc metallopeptidase STE24 (ZMPSTE24) (Homo sapiens)Mammalian Expression, Retroviral ; Bicistronic expression: puromycin resistance transcript is downstreamZMPSTE24 defends against influenza and other pathogenic viruses. J Exp Med. 2017 Feb 28. pii: jem.20161270. doi: 10.1084/jem.20161270. Human ZMPSTE24 mammalian expression vector. Retroviral bicistronic puromycin vector. pQCXIP ZMPSTE24 FACE-1, FACE1, HGPS, PRO1, STE24, Ste24p Add to Cart