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Masahito Ikawa Lab Plasmids


The Masahito Ikawa Lab has deposited plasmids at Addgene for distribution to the research community. Addgene is a nonprofit plasmid repository dedicated to improving life science research.

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Title Authors Publication
Generation of mutant mice by pronuclear injection of circular plasmid expressing Cas9 and single guided RNA. Mashiko D, Fujihara Y, Satouh Y, Miyata H, Isotani A, Ikawa M Sci Rep. 2013 Nov 27;3:3355. doi: 10.1038/srep03355.
ID Plasmid Gene/Insert Vector Type Publication Hidden Extra Search Info  
50716pCAG-EGxxFPsplit EGFP (Synthetic)Mammalian Expression, CRISPRGeneration of mutant mice by pronuclear injection of circular plasmid expressing Cas9 and single guided RNA. Sci Rep. 2013 Nov 27;3:3355. doi: 10.1038/srep03355. 5’ and 3’ EGFP fragments that shares 482bp were placed under ubiquitous CAG promoter. Used for validation of gRNA sequences by DSB mediated EGFP reconstitution. pCAGGS Add to Cart
50717pCAG-EGxxFP-Cetn1Cetn1 (Mus musculus)Mammalian Expression, CRISPRGeneration of mutant mice by pronuclear injection of circular plasmid expressing Cas9 and single guided RNA. Sci Rep. 2013 Nov 27;3:3355. doi: 10.1038/srep03355. Cetn1 genomic region was placed in pCAG-EGxxFP. Positive control for DSB mediated EGFP reconstitution. pCAG-EGxxFP Cetn1 caltractin Add to Cart
50718pX330-Cetn1/1Cetn1 sgRNA1 (Synthetic), humanized S. pyogenes Cas9 (Other)Mammalian Expression, CRISPRGeneration of mutant mice by pronuclear injection of circular plasmid expressing Cas9 and single guided RNA. Sci Rep. 2013 Nov 27;3:3355. doi: 10.1038/srep03355. pX330 containing sgRNA against mouse Cent1. Positive control for DSB mediated EGFP reconstitution. pX330 Add to Cart