Michael Rosbash Lab Plasmids


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Title Authors Publication
A role for microRNAs in the Drosophila circadian clock. Kadener S, Menet JS, Sugino K, Horwich MD, Weissbein U, Nawathean P, Vagin VV, Zamore PD, Nelson SB, Rosbash M Genes Dev. 2009 Sep 15;23(18):2179-91. Epub 2009 Aug 20.
Light-arousal and circadian photoreception circuits intersect at the large PDF cells of the Drosophila brain. Shang Y, Griffith LC, Rosbash M Proc Natl Acad Sci U S A. 2008 Dec 16;105(50):19587-94. Epub 2008 Dec 5.
The nuclear exosome and adenylation regulate posttranscriptional tethering of yeast GAL genes to the nuclear periphery. Vodala S, Abruzzi KC, Rosbash M Mol Cell. 2008 Jul 11;31(1):104-13.
Circadian transcription contributes to core period determination in Drosophila. Kadener S, Menet JS, Schoer R, Rosbash M PLoS Biol. 2008 May 20;6(5):e119.
Clockwork Orange is a transcriptional repressor and a new Drosophila circadian pacemaker component. Kadener S, Stoleru D, McDonald M, Nawathean P, Rosbash M Genes Dev. 2007 Jul 1;21(13):1675-86. Epub 2007 Jun 19.
The doubletime and CKII kinases collaborate to potentiate Drosophila PER transcriptional repressor activity. Nawathean P, Rosbash M Mol Cell. 2004 Jan 30;13(2):213-23.
T7 RNA polymerase-directed transcripts are processed in yeast and link 3' end formation to mRNA nuclear export. Dower K, Rosbash M RNA. 2002 May;8(5):686-97.
Wild-type circadian rhythmicity is dependent on closely spaced E boxes in the Drosophila timeless promoter. McDonald MJ, Rosbash M, Emery P Mol Cell Biol. 2001 Feb;21(4):1207-17.
Expression of a beta-galactosidase gene containing the ribosomal protein 51 intron is sensitive to the rna2 mutation of yeast. Teem JL, Rosbash M Proc Natl Acad Sci U S A. 1983 Jul;80(14):4403-7.
Some cis- and trans-acting mutants for splicing target pre-mRNA to the cytoplasm. Legrain P, Rosbash M Cell. 1989 May 19;57(4):573-83.
ID Plasmid Gene/Insert Vector Type Publication Hidden Extra Search Info  
33130 pRS314-T7-GFP GFP with TDH3 5' and 3' UTR Yeast Expression Dower et al RNA. 2002 May;8(5):686-97. pRS314 gfp pCmGFP_001
33131 pRRP51A+intron-LacZ (pHZ18) RP51A+intron-LacZ (Saccharomyces cerevisiae) Yeast Expression Teem et al Proc Natl Acad Sci U S A. 1983 Jul;80(14):4403-7. pLGSD5 RPS17A YML024W, RP51A, RPL51A
33132 pAC-luc-CLK CLK 3' UTR (Drosophila melanogaster) Insect Expression, Luciferase Kadener et al Genes Dev. 2009 Sep 15;23(18):2179-91. Epub 2009 Aug 20. pAc5.1/V5-His B Clk Dmel_CG7391, CG7391, CLK, CLOCK, Dmel\CG7391, Jerk, Jrk, PAS1, bHLHe10, clk, clock, dCLK, dCLK/JRK, dCLOCK, dClck, dClk, dClock, dclk, jrk
33133 pAC-luc-TimRA Tim-RA 3' UTR (Drosophila melanogaster) Insect Expression, Luciferase Kadener et al Genes Dev. 2009 Sep 15;23(18):2179-91. Epub 2009 Aug 20. pAc5.1/V5-His B tim Dmel_CG3234, CG3234, Dmel\CG3234, Ritsu, TIM, Tim, dTIM, dTim, dtim, mel_tim, rit, tim1
33143 pVS1 LacO (256 copies) Yeast Expression Vodala et al Mol Cell. 2008 Jul 11;31(1):104-13. Ylplac128
33144 pVS2 LacO (256 copies), GAL-GFP-kd200-GALpA reporter Yeast Expression Vodala et al Mol Cell. 2008 Jul 11;31(1):104-13. pVS1
33145 pLG-PLint RP51A-synthetic minimal intron (Saccharomyces cerevisiae) Yeast Expression Legrain et al Cell. 1989 May 19;57(4):573-83. pLGSD5 RPS17A YML024W, RP51A, RPL51A
33148 pLG-Acc RP51A-synthetic minimal intron (Saccharomyces cerevisiae) Yeast Expression Legrain et al Cell. 1989 May 19;57(4):573-83. pLGSD5
  • Tag / Fusion Protein
    • LacZ (C terminal on backbone)
  • RPS17A YML024W, RP51A, RPL51A
    33149 pLG-Sty RP51A-synthetic minimal intron (Saccharomyces cerevisiae) Yeast Expression Legrain et al Cell. 1989 May 19;57(4):573-83. pLGSD5
  • Tag / Fusion Protein
    • LacZ (C terminal on backbone)
  • RPS17A YML024W, RP51A, RPL51A
    33150 pLG-Nde-Acc RP51A-synthetic minimal intron (Saccharomyces cerevisiae) Yeast Expression Legrain et al Cell. 1989 May 19;57(4):573-83. pLGSD5
  • Tag / Fusion Protein
    • LacZ (C terminal on backbone)
  • RPS17A YML024W, RP51A, RPL51A
    33151 pLG-deltaA RP51A-synthetic minimal intron (Saccharomyces cerevisiae) Yeast Expression Legrain et al Cell. 1989 May 19;57(4):573-83. pLGSD5
  • Tag / Fusion Protein
    • LacZ (C terminal on backbone)
  • RPS17A YML024W, RP51A, RPL51A
    33152 pRS315-nls-T7-RNAP T7 RNAP Yeast Expression Dower et al RNA. 2002 May;8(5):686-97. pRS315 GPD
    33153 pAC-dCLK CLK (Drosophila melanogaster) Insect Expression, Retroviral McDonald et al Mol Cell Biol. 2001 Feb;21(4):1207-17. pAc5.1/V5-His B Clk Dmel_CG7391, CG7391, CLK, CLOCK, Dmel\CG7391, Jerk, Jrk, PAS1, bHLHe10, clk, clock, dCLK, dCLK/JRK, dCLOCK, dClck, dClk, dClock, dclk, jrk
    33154 pAC-Luc Insect Expression, Retroviral, Luciferase Kadener et al Genes Dev. 2007 Jul 1;21(13):1675-86. Epub 2007 Jun 19. pAc5.1/V5-His B
  • Tag / Fusion Protein
    • Luciferase (N terminal on backbone)
  • 33155 pAC-cwo cwo (Drosophila melanogaster) Insect Expression, Retroviral Kadener et al Genes Dev. 2007 Jul 1;21(13):1675-86. Epub 2007 Jun 19. pAc5.1/V5-His B cwo Dmel_CG17100, AAF24476.1, CG17100, CWO, Dmel\CG17100, GM05287, bHLHe49
    33156 pAC-Cyc Cyc (Drosophila melanogaster) Insect Expression, Retroviral Kadener et al PLoS Biol. 2008 May 20;6(5):e119. pAc5.1/V5-His A cyc Dmel_CG8727, BMAL1, Bmal1, CG8727, CYC, CYCLE, Cyc, Cycle, Dmel\CG8727, MOP3, Mop3, bHLHe7, bMAL1, bmal1, dBMAL, dBMAL1, dBma1, dBmal, dCYC, dbmal, dbmal1
    33157 pAC-Per Per (Drosophila melanogaster) Insect Expression, Retroviral Nawathean et al Mol Cell. 2004 Jan 30;13(2):213-23. pAc5.1/V5-His B per Dmel_CG2647, CG2647, Clk, Dmel\CG2647, EG:155E2.4, PER, Per, clk-6, dPER, dmper, dper, mel_per
    33259 pCasper_Pdf-LexA::VP16 LexA::VP16-SV40 3'UTR Insect Expression Shang et al Proc Natl Acad Sci U S A. 2008 Dec 16;105(50):19587-94. Epub 2008 Dec 5. pCasPeR3 lexA b4043, ECK4035, JW4003, exrA, spr, tsl, umuA