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Michael Rosbash Lab Plasmids


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Title Authors Publication
A role for microRNAs in the Drosophila circadian clock. Kadener S, Menet JS, Sugino K, Horwich MD, Weissbein U, Nawathean P, Vagin VV, Zamore PD, Nelson SB, Rosbash M Genes Dev. 2009 Sep 15;23(18):2179-91. Epub 2009 Aug 20.
Light-arousal and circadian photoreception circuits intersect at the large PDF cells of the Drosophila brain. Shang Y, Griffith LC, Rosbash M Proc Natl Acad Sci U S A. 2008 Dec 16;105(50):19587-94. Epub 2008 Dec 5.
The nuclear exosome and adenylation regulate posttranscriptional tethering of yeast GAL genes to the nuclear periphery. Vodala S, Abruzzi KC, Rosbash M Mol Cell. 2008 Jul 11;31(1):104-13.
Circadian transcription contributes to core period determination in Drosophila. Kadener S, Menet JS, Schoer R, Rosbash M PLoS Biol. 2008 May 20;6(5):e119.
Clockwork Orange is a transcriptional repressor and a new Drosophila circadian pacemaker component. Kadener S, Stoleru D, McDonald M, Nawathean P, Rosbash M Genes Dev. 2007 Jul 1;21(13):1675-86. Epub 2007 Jun 19.
The doubletime and CKII kinases collaborate to potentiate Drosophila PER transcriptional repressor activity. Nawathean P, Rosbash M Mol Cell. 2004 Jan 30;13(2):213-23.
T7 RNA polymerase-directed transcripts are processed in yeast and link 3' end formation to mRNA nuclear export. Dower K, Rosbash M RNA. 2002 May;8(5):686-97.
Wild-type circadian rhythmicity is dependent on closely spaced E boxes in the Drosophila timeless promoter. McDonald MJ, Rosbash M, Emery P Mol Cell Biol. 2001 Feb;21(4):1207-17.
Expression of a beta-galactosidase gene containing the ribosomal protein 51 intron is sensitive to the rna2 mutation of yeast. Teem JL, Rosbash M Proc Natl Acad Sci U S A. 1983 Jul;80(14):4403-7.
Some cis- and trans-acting mutants for splicing target pre-mRNA to the cytoplasm. Legrain P, Rosbash M Cell. 1989 May 19;57(4):573-83.
Plasmid Gene/Insert Vector Type Publication Hidden Extra Search Info  
pRS314-T7-GFP GFP with TDH3 5' and 3' UTR Yeast Expression T7 RNA polymerase-directed transcripts are processed in yeast and link 3' end formation to mRNA nuclear export. Dower et al RNA. 2002 May;8(5):686-97. pRS314 gfp pCmGFP_001 Add to Cart
pRRP51A+intron-LacZ (pHZ18) RP51A+intron-LacZ (Saccharomyces cerevisiae) Yeast Expression Expression of a beta-galactosidase gene containing the ribosomal protein 51 intron is sensitive to the rna2 mutation of yeast. Teem et al Proc Natl Acad Sci U S A. 1983 Jul;80(14):4403-7. pLGSD5 RPS17A YML024W, RP51A, RPL51A Add to Cart
pAC-luc-CLK CLK 3' UTR (Drosophila melanogaster) Insect Expression, Luciferase A role for microRNAs in the Drosophila circadian clock. Kadener et al Genes Dev. 2009 Sep 15;23(18):2179-91. Epub 2009 Aug 20. pAc5.1/V5-His B Clk Dmel_CG7391, CG7391, CLK, CLOCK, Dmel\CG7391, Jerk, Jrk, PAS1, bHLHe10, clk, clock, dCLK, dCLK/JRK, dCLOCK, dClck, dClk, dClock, dclk, jrk Add to Cart
pAC-luc-TimRA Tim-RA 3' UTR (Drosophila melanogaster) Insect Expression, Luciferase A role for microRNAs in the Drosophila circadian clock. Kadener et al Genes Dev. 2009 Sep 15;23(18):2179-91. Epub 2009 Aug 20. pAc5.1/V5-His B tim Dmel_CG3234, CG3234, Dmel\CG3234, Ritsu, TIM, Tim, dTIM, dTim, dtim, mel_tim, rit, tim1 Add to Cart
pVS1 LacO (256 copies) Yeast Expression The nuclear exosome and adenylation regulate posttranscriptional tethering of yeast GAL genes to the nuclear periphery. Vodala et al Mol Cell. 2008 Jul 11;31(1):104-13. Ylplac128 Add to Cart
pVS2 LacO (256 copies), GAL-GFP-kd200-GALpA reporter Yeast Expression The nuclear exosome and adenylation regulate posttranscriptional tethering of yeast GAL genes to the nuclear periphery. Vodala et al Mol Cell. 2008 Jul 11;31(1):104-13. pVS1 Add to Cart
pLG-PLint RP51A-synthetic minimal intron (Saccharomyces cerevisiae) Yeast Expression Some cis- and trans-acting mutants for splicing target pre-mRNA to the cytoplasm. Legrain et al Cell. 1989 May 19;57(4):573-83. pLGSD5 RPS17A YML024W, RP51A, RPL51A Add to Cart
pLG-Acc RP51A-synthetic minimal intron (Saccharomyces cerevisiae) Yeast Expression Some cis- and trans-acting mutants for splicing target pre-mRNA to the cytoplasm. Legrain et al Cell. 1989 May 19;57(4):573-83. pLGSD5
  • Tag / Fusion Protein
    • LacZ (C terminal on backbone)
  • RPS17A YML024W, RP51A, RPL51A
    Add to Cart
    pLG-Sty RP51A-synthetic minimal intron (Saccharomyces cerevisiae) Yeast Expression Some cis- and trans-acting mutants for splicing target pre-mRNA to the cytoplasm. Legrain et al Cell. 1989 May 19;57(4):573-83. pLGSD5
  • Tag / Fusion Protein
    • LacZ (C terminal on backbone)
  • RPS17A YML024W, RP51A, RPL51A
    Add to Cart
    pLG-Nde-Acc RP51A-synthetic minimal intron (Saccharomyces cerevisiae) Yeast Expression Some cis- and trans-acting mutants for splicing target pre-mRNA to the cytoplasm. Legrain et al Cell. 1989 May 19;57(4):573-83. pLGSD5
  • Tag / Fusion Protein
    • LacZ (C terminal on backbone)
  • RPS17A YML024W, RP51A, RPL51A
    Add to Cart
    pLG-deltaA RP51A-synthetic minimal intron (Saccharomyces cerevisiae) Yeast Expression Some cis- and trans-acting mutants for splicing target pre-mRNA to the cytoplasm. Legrain et al Cell. 1989 May 19;57(4):573-83. pLGSD5
  • Tag / Fusion Protein
    • LacZ (C terminal on backbone)
  • RPS17A YML024W, RP51A, RPL51A
    Add to Cart
    pRS315-nls-T7-RNAP T7 RNAP Yeast Expression T7 RNA polymerase-directed transcripts are processed in yeast and link 3' end formation to mRNA nuclear export. Dower et al RNA. 2002 May;8(5):686-97. pRS315 GPD Add to Cart
    pAC-dCLK CLK (Drosophila melanogaster) Insect Expression, Retroviral Wild-type circadian rhythmicity is dependent on closely spaced E boxes in the Drosophila timeless promoter. McDonald et al Mol Cell Biol. 2001 Feb;21(4):1207-17. pAc5.1/V5-His B Clk Dmel_CG7391, CG7391, CLK, CLOCK, Dmel\CG7391, Jerk, Jrk, PAS1, bHLHe10, clk, clock, dCLK, dCLK/JRK, dCLOCK, dClck, dClk, dClock, dclk, jrk Add to Cart
    pAC-Luc Insect Expression, Retroviral, Luciferase Clockwork Orange is a transcriptional repressor and a new Drosophila circadian pacemaker component. Kadener et al Genes Dev. 2007 Jul 1;21(13):1675-86. Epub 2007 Jun 19. pAc5.1/V5-His B
  • Tag / Fusion Protein
    • Luciferase (N terminal on backbone)
  • Add to Cart
    pAC-cwo cwo (Drosophila melanogaster) Insect Expression, Retroviral Clockwork Orange is a transcriptional repressor and a new Drosophila circadian pacemaker component. Kadener et al Genes Dev. 2007 Jul 1;21(13):1675-86. Epub 2007 Jun 19. pAc5.1/V5-His B cwo Dmel_CG17100, AAF24476.1, CG17100, CWO, Dmel\CG17100, GM05287, bHLHe49 Add to Cart
    pAC-Cyc Cyc (Drosophila melanogaster) Insect Expression, Retroviral Circadian transcription contributes to core period determination in Drosophila. Kadener et al PLoS Biol. 2008 May 20;6(5):e119. pAc5.1/V5-His A cyc Dmel_CG8727, BMAL1, Bmal1, CG8727, CYC, CYCLE, Cyc, Cycle, Dmel\CG8727, MOP3, Mop3, bHLHe7, bMAL1, bmal1, dBMAL, dBMAL1, dBma1, dBmal, dCYC, dbmal, dbmal1 Add to Cart
    pAC-Per Per (Drosophila melanogaster) Insect Expression, Retroviral The doubletime and CKII kinases collaborate to potentiate Drosophila PER transcriptional repressor activity. Nawathean et al Mol Cell. 2004 Jan 30;13(2):213-23. pAc5.1/V5-His B per Dmel_CG2647, CG2647, Clk, Dmel\CG2647, EG:155E2.4, PER, Per, clk-6, dPER, dmper, dper, mel_per Add to Cart
    pCasper_Pdf-LexA::VP16 LexA::VP16-SV40 3'UTR Insect Expression Light-arousal and circadian photoreception circuits intersect at the large PDF cells of the Drosophila brain. Shang et al Proc Natl Acad Sci U S A. 2008 Dec 16;105(50):19587-94. Epub 2008 Dec 5. pCasPeR3 lexA b4043, ECK4035, JW4003, exrA, spr, tsl, umuA Add to Cart