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Michael Ruppert Lab Plasmids


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Title Authors Publication
SOX9 inhibits beta-TrCP-mediated protein degradation to promote nuclear GLI1 expression and cancer stem cell properties. Deng W, Vanderbilt DB, Lin CC, Martin KH, Brundage KM, Ruppert JM J Cell Sci. 2015 Jan 27. pii: jcs.162164.
MicroRNAs 206 and 21 cooperate to promote RAS-extracellular signal-regulated kinase signaling by suppressing the translation of RASA1 and SPRED1. Sharma SB, Lin CC, Farrugia MK, McLaughlin SL, Ellis EJ, Brundage KM, Salkeni MA, Ruppert JM Mol Cell Biol. 2014 Nov 15;34(22):4143-64. doi: 10.1128/MCB.00480-14. Epub 2014 Sep 8.
A KLF4-miRNA-206 autoregulatory feedback loop can promote or inhibit protein translation depending upon cell context. Lin CC, Liu LZ, Addison JB, Wonderlin WF, Ivanov AV, Ruppert JM Mol Cell Biol. 2011 Jun;31(12):2513-27. Epub 2011 Apr 25.
Induction of KLF4 in basal keratinocytes blocks the proliferation-differentiation switch and initiates squamous epithelial dysplasia. Foster KW, Liu Z, Nail CD, Li X, Fitzgerald TJ, Bailey SK, Frost AR, Louro ID, Townes TM, Paterson AJ, Kudlow JE, Lobo-Ruppert SM, Ruppert JM Oncogene. 2005 Feb 24;24(9):1491-500.
ID Plasmid Gene/Insert Vector Type Publication Hidden Extra Search Info  
34589 pBpuro - HA KLF4 FL KLF4 (Homo sapiens) Mammalian Expression, Retroviral A KLF4-miRNA-206 autoregulatory feedback loop can promote or inhibit protein translation depending upon cell context. Mol Cell Biol. 2011 Jun;31(12):2513-27. Epub 2011 Apr 25. pBABE-puro KLF4 EZF, GKLF Add to Cart
34592 pBpuro - HA KLF4 FL delta K5S KLF4 (Homo sapiens) Mammalian Expression, Retroviral A KLF4-miRNA-206 autoregulatory feedback loop can promote or inhibit protein translation depending upon cell context. Mol Cell Biol. 2011 Jun;31(12):2513-27. Epub 2011 Apr 25. pBABE-puro KLF4 EZF, GKLF Add to Cart
34593 pcDNA3.1 - HA-KLF4 FL KLF4 (Homo sapiens) Mammalian Expression A KLF4-miRNA-206 autoregulatory feedback loop can promote or inhibit protein translation depending upon cell context. Mol Cell Biol. 2011 Jun;31(12):2513-27. Epub 2011 Apr 25. pcDNA3.1+ KLF4 EZF, GKLF Add to Cart
34594 pcDNA3.1 - HA-KLF4 FL delta K5S KLF4 (Homo sapiens) Mammalian Expression A KLF4-miRNA-206 autoregulatory feedback loop can promote or inhibit protein translation depending upon cell context. Mol Cell Biol. 2011 Jun;31(12):2513-27. Epub 2011 Apr 25. pcDNA3.1+ KLF4 EZF, GKLF Add to Cart
34595 pLJD vector Mammalian Expression, Retroviral A KLF4-miRNA-206 autoregulatory feedback loop can promote or inhibit protein translation depending upon cell context. Mol Cell Biol. 2011 Jun;31(12):2513-27. Epub 2011 Apr 25. pLJD Add to Cart
34596 pLJD-KLF4-ER KLF4 (Homo sapiens) Mammalian Expression, Retroviral A KLF4-miRNA-206 autoregulatory feedback loop can promote or inhibit protein translation depending upon cell context. Mol Cell Biol. 2011 Jun;31(12):2513-27. Epub 2011 Apr 25. pLJD KLF4 EZF, GKLF Add to Cart
34597 pMIR-Report-Luc-KLF4-FL KLF4 (Homo sapiens) Mammalian Expression, Luciferase A KLF4-miRNA-206 autoregulatory feedback loop can promote or inhibit protein translation depending upon cell context. Mol Cell Biol. 2011 Jun;31(12):2513-27. Epub 2011 Apr 25. pMIR-Report-LUC KLF4 EZF, GKLF Add to Cart
34598 pMIR-Report-Luc-KLF4-K5S KLF4 (Homo sapiens) Mammalian Expression, Luciferase A KLF4-miRNA-206 autoregulatory feedback loop can promote or inhibit protein translation depending upon cell context. Mol Cell Biol. 2011 Jun;31(12):2513-27. Epub 2011 Apr 25. pMIR-Report-LUC KLF4 EZF, GKLF Add to Cart
34599 pMIR-Report-Luc-KLF4-K5S-Mt206A KLF4 (Homo sapiens) Mammalian Expression, Luciferase A KLF4-miRNA-206 autoregulatory feedback loop can promote or inhibit protein translation depending upon cell context. Mol Cell Biol. 2011 Jun;31(12):2513-27. Epub 2011 Apr 25. pMIR-Report-LUC KLF4 EZF, GKLF Add to Cart
34600 pMIR-Report-Luc-KLF4-K5S-Mt206B KLF4 (Homo sapiens) Mammalian Expression, Luciferase A KLF4-miRNA-206 autoregulatory feedback loop can promote or inhibit protein translation depending upon cell context. Mol Cell Biol. 2011 Jun;31(12):2513-27. Epub 2011 Apr 25. pMIR-Report-LUC KLF4 EZF, GKLF Add to Cart
34601 pMIR-Report-Luc-KLF4-K5S-Mt344A KLF4 (Homo sapiens) Mammalian Expression, Luciferase A KLF4-miRNA-206 autoregulatory feedback loop can promote or inhibit protein translation depending upon cell context. Mol Cell Biol. 2011 Jun;31(12):2513-27. Epub 2011 Apr 25. pMIR-Report-LUC KLF4 EZF, GKLF Add to Cart
34602 pMIR-Report-Luc-KLF4-K5S-Mt344B KLF4 (Homo sapiens) Mammalian Expression, Luciferase A KLF4-miRNA-206 autoregulatory feedback loop can promote or inhibit protein translation depending upon cell context. Mol Cell Biol. 2011 Jun;31(12):2513-27. Epub 2011 Apr 25. pMIR-Report-LUC KLF4 EZF, GKLF Add to Cart
34609 pBpuro_KLF4_ER KLF4 (Homo sapiens) Mammalian Expression, Retroviral Induction of KLF4 in basal keratinocytes blocks the proliferation-differentiation switch and initiates squamous epithelial dysplasia. Oncogene. 2005 Feb 24;24(9):1491-500. pBABE-puro
  • Tags / Fusion Proteins
    • Myc epitope tag (N terminal on insert)
    • estrogen receptor fusion (C terminal on insert)
  • KLF4 EZF, GKLF
    Add to Cart
    62575 pMIR-Report-RASA1-3'UTR RASA1 (Homo sapiens) Luciferase MicroRNAs 206 and 21 cooperate to promote RAS-extracellular signal-regulated kinase signaling by suppressing the translation of RASA1 and SPRED1. Mol Cell Biol. 2014 Nov 15;34(22):4143-64. doi: 10.1128/MCB.00480-14. Epub 2014 Sep 8. Translational Luciferase Reporter containing a 926 bp fragment of the RASA1 3'UTR. pMIR-Report RASA1 CM-AVM, CMAVM, GAP, PKWS, RASA, RASGAP, p120GAP, p120RASGAP Add to Cart
    62576 pMIR-Report-RASA1-3'UTR (mut - miR-206 site) RASA1 (Homo sapiens) Luciferase MicroRNAs 206 and 21 cooperate to promote RAS-extracellular signal-regulated kinase signaling by suppressing the translation of RASA1 and SPRED1. Mol Cell Biol. 2014 Nov 15;34(22):4143-64. doi: 10.1128/MCB.00480-14. Epub 2014 Sep 8. Translational Luciferase Reporter containing the mutated 3'UTR of RASA1. The miR-206 binding site was mutated. pMIR-Report RASA1 CM-AVM, CMAVM, GAP, PKWS, RASA, RASGAP, p120GAP, p120RASGAP Add to Cart
    62577 pMIR-Report-SPRED1-3'UTR (WT - miR-206 site) SPRED1 (Homo sapiens) Luciferase MicroRNAs 206 and 21 cooperate to promote RAS-extracellular signal-regulated kinase signaling by suppressing the translation of RASA1 and SPRED1. Mol Cell Biol. 2014 Nov 15;34(22):4143-64. doi: 10.1128/MCB.00480-14. Epub 2014 Sep 8. Translational Luciferase Reporter containing a fragment of the 3'UTR of SPRED1 containing a miR-206 binding site pMIR-Report SPRED1 NFLS, PPP1R147, hSpred1, spred-1 Add to Cart
    62579 pMIR-Report-SPRED1-3'UTR (containing miR-21 sites) SPRED1 (Homo sapiens) Luciferase MicroRNAs 206 and 21 cooperate to promote RAS-extracellular signal-regulated kinase signaling by suppressing the translation of RASA1 and SPRED1. Mol Cell Biol. 2014 Nov 15;34(22):4143-64. doi: 10.1128/MCB.00480-14. Epub 2014 Sep 8. Translational Luciferase Reporter encoding a fragment of the 3'UTR of SPRED1 containing two miR-21 binding sites (sites 1 and 2) pMIR-Report SPRED1 NFLS, PPP1R147, hSpred1, spred-1 Add to Cart
    62580 pMIR-Report-SPRED1-3'UTR (mut - miR-21 site1) SPRED1 (Homo sapiens) Luciferase MicroRNAs 206 and 21 cooperate to promote RAS-extracellular signal-regulated kinase signaling by suppressing the translation of RASA1 and SPRED1. Mol Cell Biol. 2014 Nov 15;34(22):4143-64. doi: 10.1128/MCB.00480-14. Epub 2014 Sep 8. Translational Luciferase Reporter encoding a mutated fragment of the SPRED1 3'UTR. The upstream miR-21 (site 1) binding site was mutated. pMIR-Report SPRED1 NFLS, PPP1R147, hSpred1, spred-1 Add to Cart
    62581 pMIR-Report-SPRED1-3'UTR (mut - miR-21 site 2) SPRED1 (Homo sapiens) Luciferase MicroRNAs 206 and 21 cooperate to promote RAS-extracellular signal-regulated kinase signaling by suppressing the translation of RASA1 and SPRED1. Mol Cell Biol. 2014 Nov 15;34(22):4143-64. doi: 10.1128/MCB.00480-14. Epub 2014 Sep 8. Translational Luciferase Reporter encoding a mutated fragment of the SPRED1 3'UTR. The downstream miR-21 binding site (site 2) was mutated. pMIR-Report SPRED1 NFLS, PPP1R147, hSpred1, spred-1 Add to Cart
    62967 pBABEPuro-HA-GLI1 GLI1 (Homo sapiens) Retroviral SOX9 inhibits beta-TrCP-mediated protein degradation to promote nuclear GLI1 expression and cancer stem cell properties. J Cell Sci. 2015 Jan 27. pii: jcs.162164. Expresses HA-tagged Gli1 pBABE-puro GLI1 GLI Add to Cart
    62972 pcDNA3.1 HA-rnSOX9 SOX9 (Rattus norvegicus) Mammalian Expression SOX9 inhibits beta-TrCP-mediated protein degradation to promote nuclear GLI1 expression and cancer stem cell properties. J Cell Sci. 2015 Jan 27. pii: jcs.162164. Expresses HA-tagged rat SOX9 pcDNA3.1 Sox9 Add to Cart
    62973 pcDNA3.1 HA-rnSOX9 ΔC (1-381) SOX9 (Rattus norvegicus) Mammalian Expression SOX9 inhibits beta-TrCP-mediated protein degradation to promote nuclear GLI1 expression and cancer stem cell properties. J Cell Sci. 2015 Jan 27. pii: jcs.162164. Expresses HA-tagged rat SOX9 residues 1-383 pcDNA3.1 Sox9 Add to Cart
    62975 pcDNA3.1 HA-rnSOX9 PQA/S (286-507) SOX9 (Rattus norvegicus) Mammalian Expression SOX9 inhibits beta-TrCP-mediated protein degradation to promote nuclear GLI1 expression and cancer stem cell properties. J Cell Sci. 2015 Jan 27. pii: jcs.162164. Expresses HA-tagged rat SOX9 residues 286-507 pcDNA3.1 Sox9 Add to Cart
    62976 pcDNA3.1 myc-mβTrCP F-box (1-247) BTRC (Mus musculus) Mammalian Expression SOX9 inhibits beta-TrCP-mediated protein degradation to promote nuclear GLI1 expression and cancer stem cell properties. J Cell Sci. 2015 Jan 27. pii: jcs.162164. Expresses myc-tagged mouse βTrCP residues 1-247 pcDNA3.1 Btrc Beta-Trcp1, E3RS-IkappaB, E3RSIkappaB, FWD1, Fbw1a, HOS, Slimb, b-TrCP, beta-TrCP Add to Cart
    62977 pcDNA3.1 myc-mβTrCP WD1-7 (241-569) BTRC (Mus musculus) Mammalian Expression SOX9 inhibits beta-TrCP-mediated protein degradation to promote nuclear GLI1 expression and cancer stem cell properties. J Cell Sci. 2015 Jan 27. pii: jcs.162164. Expresses myc-tagged mouse βTrCP residues 241-569 pcDNA3.1 Btrc Beta-Trcp1, E3RS-IkappaB, E3RSIkappaB, FWD1, Fbw1a, HOS, Slimb, b-TrCP, beta-TrCP Add to Cart
    62978 pcDNA3.1 myc-mβTrCP WD2-7 (292-569) BTRC (Mus musculus) Mammalian Expression SOX9 inhibits beta-TrCP-mediated protein degradation to promote nuclear GLI1 expression and cancer stem cell properties. J Cell Sci. 2015 Jan 27. pii: jcs.162164. Expresses myc-tagged mouse βTrCP residues 292-569 pcDNA3.1 Btrc Beta-Trcp1, E3RS-IkappaB, E3RSIkappaB, FWD1, Fbw1a, HOS, Slimb, b-TrCP, beta-TrCP Add to Cart
    62979 pMIR-Report-Luc-KLF4-K1S KLF4 partial sequence (Homo sapiens) Luciferase A KLF4-miRNA-206 autoregulatory feedback loop can promote or inhibit protein translation depending upon cell context. Mol Cell Biol. 2011 Jun;31(12):2513-27. Epub 2011 Apr 25. Translational reporter for KLF4 pMIR-Report-LUC KLF4 EZF, GKLF Add to Cart
    62980 pMIR-Report-Luc-KLF4-K2S KLF4 partial sequence (Homo sapiens) Luciferase A KLF4-miRNA-206 autoregulatory feedback loop can promote or inhibit protein translation depending upon cell context. Mol Cell Biol. 2011 Jun;31(12):2513-27. Epub 2011 Apr 25. Translational reporter for KLF4 pMIR-Report-LUC KLF4 EZF, GKLF Add to Cart
    62981 pMIR-Report-Luc-KLF4-K3S KLF4 partial sequence (Homo sapiens) Luciferase A KLF4-miRNA-206 autoregulatory feedback loop can promote or inhibit protein translation depending upon cell context. Mol Cell Biol. 2011 Jun;31(12):2513-27. Epub 2011 Apr 25. Translational reporter for KLF4 pMIR-Report-LUC KLF4 EZF, GKLF Add to Cart
    62982 pMIR-Report-Luc-KLF4-K4S KLF4 partial sequence (Homo sapiens) Luciferase A KLF4-miRNA-206 autoregulatory feedback loop can promote or inhibit protein translation depending upon cell context. Mol Cell Biol. 2011 Jun;31(12):2513-27. Epub 2011 Apr 25. Translational reporter for KLF4 pMIR-Report-LUC KLF4 EZF, GKLF Add to Cart
    62983 pMIR-Report-Luc-KLF4-K6S KLF4 partial sequence (Homo sapiens) Luciferase A KLF4-miRNA-206 autoregulatory feedback loop can promote or inhibit protein translation depending upon cell context. Mol Cell Biol. 2011 Jun;31(12):2513-27. Epub 2011 Apr 25. Translational reporter for KLF4 pMIR-Report-LUC KLF4 EZF, GKLF Add to Cart
    63009 pBABEPuro-rnSOX9 SOX9 (Rattus norvegicus) Retroviral SOX9 inhibits beta-TrCP-mediated protein degradation to promote nuclear GLI1 expression and cancer stem cell properties. J Cell Sci. 2015 Jan 27. pii: jcs.162164. Expresses rat SOX9 pBABE-puro Sox9 Add to Cart