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Mikel Zaratiegui Lab Plasmids


The Mikel Zaratiegui Lab has deposited plasmids at Addgene for distribution to the research community. Addgene is a nonprofit plasmid repository dedicated to improving life science research.

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Title Authors Publication
Implementation of the CRISPR-Cas9 system in fission yeast. Jacobs JZ, Ciccaglione KM, Tournier V, Zaratiegui M Nat Commun. 2014 Oct 29;5:5344. doi: 10.1038/ncomms6344.
sgRNA/Cas9 (unpublished)
ID Plasmid Gene/Insert Vector Type Publication Hidden Extra Search Info  
52223pMZ222Cas9 (Other)Yeast ExpressionImplementation of the CRISPR-Cas9 system in fission yeast. Nat Commun. 2014 Oct 29;5:5344. doi: 10.1038/ncomms6344. Constitutive expression of humanized Cas9 pART1 Add to Cart
52224pMZ283rrk1-sgRNA (Schizosaccharomyces pombe)Yeast Expression, CRISPRImplementation of the CRISPR-Cas9 system in fission yeast. Nat Commun. 2014 Oct 29;5:5344. doi: 10.1038/ncomms6344. Expression of sgRNA precursor with CspCI placeholder for target cloning (see comments). pUR18 Add to Cart
52225pMZ284rrk1-sgRNA (Schizosaccharomyces pombe)Yeast Expression, CRISPRImplementation of the CRISPR-Cas9 system in fission yeast. Nat Commun. 2014 Oct 29;5:5344. doi: 10.1038/ncomms6344. Expression of sgRNA precursor targeted against ade6+ pMZ283 Add to Cart
52226pMZ285rrk1-sgRNA (Schizosaccharomyces pombe)Yeast Expression, CRISPRImplementation of the CRISPR-Cas9 system in fission yeast. Nat Commun. 2014 Oct 29;5:5344. doi: 10.1038/ncomms6344. Expression of sgRNA precursor targeted against ade6M210 pMZ283 Add to Cart
52227pMZ286rrk1-sgRNA (Schizosaccharomyces pombe)Yeast Expression, CRISPRImplementation of the CRISPR-Cas9 system in fission yeast. Nat Commun. 2014 Oct 29;5:5344. doi: 10.1038/ncomms6344. Expression of sgRNA precursor targeted against ade6L469 pMZ283 Add to Cart
59896pMZ374Cas9 (Other), rrk1:sgRNA (Other)Yeast Expression, CRISPRImplementation of the CRISPR-Cas9 system in fission yeast. Nat Commun. 2014 Oct 29;5:5344. doi: 10.1038/ncomms6344. Combination adh1:cas9/rrk1:sgRNA for CRISPR genome editing in fission yeast: Empty sgRNA target (CspCI placeholder) (see comments). pMZ283 Add to Cart
59897pMZ288Cas9 (Other), rrk1:sgRNA (Other)Yeast Expression, CRISPRImplementation of the CRISPR-Cas9 system in fission yeast. Nat Commun. 2014 Oct 29;5:5344. doi: 10.1038/ncomms6344. Combination adh1:cas9/rrk1:sgRNA for CRISPR genome editing in fission yeast: sgRNA targeted against ade6+ pMZ283 Add to Cart
59898pMZ289Cas9 (Other), rrk1:sgRNA (Other)Yeast Expression, CRISPRImplementation of the CRISPR-Cas9 system in fission yeast. Nat Commun. 2014 Oct 29;5:5344. doi: 10.1038/ncomms6344. Combination adh1:cas9/rrk1:sgRNA for CRISPR genome editing in fission yeast: sgRNA targeted against ade6-M210 pMZ283 Add to Cart
59899pMZ381Cas9 (Other), rrk1:sgRNA (Other)Yeast Expression, CRISPRImplementation of the CRISPR-Cas9 system in fission yeast. Nat Commun. 2014 Oct 29;5:5344. doi: 10.1038/ncomms6344. Combination adh1:cas9/rrk1:sgRNA for CRISPR genome editing in fission yeast: sgRNA targeted against ade6-L469 pMZ283 Add to Cart
74213pMZ376Cas9 (Other), rrk1:sgRNA (Other)Yeast Expression, CRISPR sgRNA/Cas9 (unpublished) Combined sgRNA/Cas9 vector with KanMX marker pMZ374 Add to Cart
74214pMZ377Cas9 (Other), rrk1:sgRNA (Other)Yeast Expression, CRISPR sgRNA/Cas9 (unpublished) Combined sgRNA/Cas9 vector with LEU2 marker pMZ374 Add to Cart
74215pMZ379Cas9 (Other), rrk1:sgRNA (Other)Yeast Expression, CRISPR sgRNA/Cas9 (unpublished) Combined sgRNA/Cas9 vector with NatMX marker pMZ374 Add to Cart