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Paul Sternberg Lab Plasmids


The Paul Sternberg Lab has deposited plasmids at Addgene for distribution to the research community. Addgene is a nonprofit plasmid repository dedicated to improving life science research.

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Title Authors Publication
Visualization of C. elegans transgenic arrays by GFP. Gonzalez-Serricchio AS, Sternberg PW BMC Genet. 2006 Jun 7;7:36.
Opposing Wnt pathways orient cell polarity during organogenesis. Green JL, Inoue T, Sternberg PW Cell. 2008 Aug 22;134(4):646-56.
Transgene-Free Genome Editing in Caenorhabditis elegans Using CRISPR-Cas. Chiu H, Schwartz HT, Antoshechkin I, Sternberg PW Genetics. 2013 Aug 26.
cGAL, a temperature-robust GAL4-UAS system for Caenorhabditis elegans. Wang H, Liu J, Gharib S, Chai CM, Schwarz EM, Pokala N, Sternberg PW Nat Methods. 2016 Dec 19. doi: 10.1038/nmeth.4109.
ID Plasmid Gene/Insert Vector Type Publication Hidden Extra Search Info  
34848POPTOPTCF binding sitesBacterial ExpressionOpposing Wnt pathways orient cell polarity during organogenesis. Cell. 2008 Aug 22;134(4):646-56. pJIM20 Add to Cart
34849POPFOPMutated TCF binding sitesBacterial ExpressionOpposing Wnt pathways orient cell polarity during organogenesis. Cell. 2008 Aug 22;134(4):646-56. pJIM20
  • Tags / Fusion Proteins
    • mCherry (C terminal on backbone)
    • let-858 3'UTR (C terminal on backbone)
  • Add to Cart
    34853pTY1048-S65T-LacIGFP-LacIWorm ExpressionVisualization of C. elegans transgenic arrays by GFP. BMC Genet. 2006 Jun 7;7:36. pTY1048 Add to Cart
    47911SP6-hCas9-Ce-mRNAhCas9 with C. elegans 5' and 3' UTRs (Homo sapiens)CRISPRTransgene-Free Genome Editing in Caenorhabditis elegans Using CRISPR-Cas. Genetics. 2013 Aug 26. In vitro transcription of a mRNA encoding humanized Cas9 nuclease, with 3' UTRs suitable for germline expression in C. elegans, for doing CRISPR-Cas by RNA injection. Uses SP6 RNA polymerase. Bluescript Add to Cart
    47912SP6-sgRNA-scaffoldWorm Expression, CRISPRTransgene-Free Genome Editing in Caenorhabditis elegans Using CRISPR-Cas. Genetics. 2013 Aug 26. Scaffold into which clone a 20 bp targeting sequence to generate a plasmid for in vitro transcription of an sgRNA using SP6 RNA polymerase for use in CRISPR-Cas by RNA injection Bluescript
  • Tag / Fusion Protein
    • sgRNA 3' end (C terminal on insert)
  • Add to Cart
    85583pHW393 (Prab-3::GAL4-SK(DBD)::VP64::let-858 3'UTR)Prab-3-GAL4-SK(DBD)-VP64Worm ExpressioncGAL, a temperature-robust GAL4-UAS system for Caenorhabditis elegans. Nat Methods. 2016 Dec 19. doi: 10.1038/nmeth.4109. cGAL driver (Prab-3::GAL4-SK(DBD)::VP64::let-858 3'UTR) construct for C. elegans using the S. kudriavzevii (SK) GAL4 DNA-binding domain (DBD) pPD117.01 Add to Cart
    85584pHW394 (15xUAS::GFP::let-858 3'UTR)15xUAS-delta pes-10-GFP (Caenorhabditis elegans)Worm ExpressioncGAL, a temperature-robust GAL4-UAS system for Caenorhabditis elegans. Nat Methods. 2016 Dec 19. doi: 10.1038/nmeth.4109. cGAL effector (15xUAS::GFP::let-858 3'UTR) construct for C. elegans pPD117.01 Add to Cart