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Peter Hegemann Lab Plasmids


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Title Authors Publication
Light modulation of cellular cAMP by a small bacterial photoactivated adenylyl cyclase, bPAC, of the soil bacterium Beggiatoa. Stierl M, Stumpf P, Udwari D, Gueta R, Hagedorn R, Losi A, Gartner W, Petereit L, Efetova M, Schwarzel M, Oertner TG, Nagel G, Hegemann P J Biol Chem. 2011 Jan 14. 286(2):1181-8.
Identification of a Natural Green Light Absorbing Chloride Conducting Channelrhodopsin from Proteomonas sulcata. Wietek J, Broser M, Krause BS, Hegemann P J Biol Chem. 2016 Feb 19;291(8):4121-7. doi: 10.1074/jbc.M115.699637. Epub 2016 Jan 6.
An improved chloride-conducting channelrhodopsin for light-induced inhibition of neuronal activity in vivo. Wietek J, Beltramo R, Scanziani M, Hegemann P, Oertner TG, Simon Wiegert J Sci Rep. 2015 Oct 7;5:14807. doi: 10.1038/srep14807.
Conversion of Channelrhodopsin into a Light-Gated Chloride Channel. Wietek J, Wiegert JS, Adeishvili N, Schneider F, Watanabe H, Tsunoda SP, Vogt A, Elstner M, Oertner TG, Hegemann P Science. 2014 Mar 27.
The rhodopsin-guanylyl cyclase of the aquatic fungus Blastocladiella emersonii enables fast optical control of cGMP signaling. Scheib U, Stehfest K, Gee CE, Korschen HG, Fudim R, Oertner TG, Hegemann P Sci Signal. 2015 Aug 11;8(389):rs8. doi: 10.1126/scisignal.aab0611.
Artificial anion-conducting channelrhodopsins with tuned spectra, modified kinetics and enhanced light sensitivity Wietek, Jonas, Silvia Rodriguez-Rozada, Janine Tutas, Federico Tenedini, Christiane Grimm, Thomas Oertner, Peter Soba, Peter Hegemann, J. Simon Wiegert biorxiv
ID Plasmid Gene/Insert Vector Type Publication Hidden Extra Search Info  
28134pGEM-HE-h_bPAC_cmychumanized photoactivated adenylyl cyclasein vitro transcriptionLight modulation of cellular cAMP by a small bacterial photoactivated adenylyl cyclase, bPAC, of the soil bacterium Beggiatoa. J Biol Chem. 2011 Jan 14. 286(2):1181-8. pGEM-HE Add to Cart
28135pET28a-ec_bPACE.coli adapted photoactivated adenylyl cyclaseBacterial ExpressionLight modulation of cellular cAMP by a small bacterial photoactivated adenylyl cyclase, bPAC, of the soil bacterium Beggiatoa. J Biol Chem. 2011 Jan 14. 286(2):1181-8. pET28a Add to Cart
85461p-mCherry-N1-ChloC ChloC (Synthetic)Mammalian ExpressionConversion of Channelrhodopsin into a Light-Gated Chloride Channel. Science. 2014 Mar 27. Artificial anion conducting ChR2 variant with fast kinetics. Codon optimized for mammalian expression (human/mouse) pEGFP-N1 Add to Cart
85462p-mCherry-N1-slowChloC slowChloC (Synthetic)Mammalian ExpressionConversion of Channelrhodopsin into a Light-Gated Chloride Channel. Science. 2014 Mar 27. Artificial anion conducting ChR2 variant with slow kinetics and high light sensitivity . Codon optimized for mammalian expression (human/mouse) pEGFP-N1 Add to Cart
85463p-mCherry-C1-GtACR2Synthetic construct ACR2 gene (Synthetic)Mammalian ExpressionIdentification of a Natural Green Light Absorbing Chloride Conducting Channelrhodopsin from Proteomonas sulcata. J Biol Chem. 2016 Feb 19;291(8):4121-7. doi: 10.1074/jbc.M115.699637. Epub 2016 Jan 6. Natural anion conducting Channelrhodopsin with blue absorption. Closing kinetics 60ms. High conductivity. Codon optimized for mammalian expression (human/mouse) pEGFP-C1 Add to Cart
85464p-mCherry-C1-GtACR1Synthetic construct ACR1 gene (Synthetic)Mammalian ExpressionIdentification of a Natural Green Light Absorbing Chloride Conducting Channelrhodopsin from Proteomonas sulcata. J Biol Chem. 2016 Feb 19;291(8):4121-7. doi: 10.1074/jbc.M115.699637. Epub 2016 Jan 6. Natural anion conducting Channelrhodopsin with cyan absorption. Closing kinetics 140ms. High conductivity. Codon optimized for mammalian expression (human/mouse) pEGFP-C1 Add to Cart
85465p-mCherry-C1-PsACR1Synthetic construct PsChR1 gene (Synthetic)Mammalian ExpressionIdentification of a Natural Green Light Absorbing Chloride Conducting Channelrhodopsin from Proteomonas sulcata. J Biol Chem. 2016 Feb 19;291(8):4121-7. doi: 10.1074/jbc.M115.699637. Epub 2016 Jan 6. Natural anion conducting Channelrhodopsin with red shifted absorption. Closing kinetics 80ms. Codon optimized for mammalian expression (human/mouse) pEGFP-C1 Add to Cart
85467p-mCherry-N1-iChloCiChloC (Synthetic)Mammalian ExpressionAn improved chloride-conducting channelrhodopsin for light-induced inhibition of neuronal activity in vivo. Sci Rep. 2015 Oct 7;5:14807. doi: 10.1038/srep14807. improved slowChloC artificial anion conducting ChR. Codon optimized for mammalian expression (human/mouse) pEGFP-N1 Add to Cart
85468bPAC-mycHis_pGEMBacterial Photoactivated Adenylyl Cyclase (Synthetic)Mammalian ExpressionLight modulation of cellular cAMP by a small bacterial photoactivated adenylyl cyclase, bPAC, of the soil bacterium Beggiatoa. J Biol Chem. 2011 Jan 14. 286(2):1181-8. Expression of photoactivated cyclase bPAC of Beggiatoa sp. pGEM Add to Cart
85469RhGC(BE)_pGEMrhodopsin-guanylyl cyclase (Synthetic)Mammalian ExpressionThe rhodopsin-guanylyl cyclase of the aquatic fungus Blastocladiella emersonii enables fast optical control of cGMP signaling. Sci Signal. 2015 Aug 11;8(389):rs8. doi: 10.1126/scisignal.aab0611. The rhodopsin-guanylyl cyclase of the aquatic fungus Blastocladiella emersonii enables fast optical control of cGMP signaling pGEM Add to Cart
98165p-mCherry-C1-iC++Synthetic construct iC++ gene (Synthetic)Mammalian ExpressionArtificial anion-conducting channelrhodopsins with tuned spectra, modified kinetics and enhanced light sensitivity biorxiv artificial anion conducting channelrhodopsin (aACR). Activation max 488 nm; off-kinetics 35 ms. Codon optimized for mammalian expression. pEGFP-C1
  • Tag / Fusion Protein
    • mCherry (C terminal on backbone)
  • Add to Cart
    98166p-mCherry-C1-PhobosSynthetic construct Phobos gene (Synthetic)Mammalian ExpressionArtificial anion-conducting channelrhodopsins with tuned spectra, modified kinetics and enhanced light sensitivity biorxiv blue-shifted artificial anion conducting channelrhodopsin (aACR). Activation max 466 nm; off-kinetics 10 ms. Codon optimized for mammalian expression. pEGFP-C1
  • Tag / Fusion Protein
    • mCherry (C terminal on backbone)
  • Add to Cart
    98167p-mCherry-C1-AuroraSynthetic construct Aurora gene (Synthetic)Mammalian ExpressionArtificial anion-conducting channelrhodopsins with tuned spectra, modified kinetics and enhanced light sensitivity biorxiv red-shifted artificial anion conducting channelrhodopsin (aACR). Activation up to 600 nm; off-kinetics 260 ms. Codon optimized for mammalian expression. pEGFP-C1
  • Tag / Fusion Protein
    • mCherry (C terminal on backbone)
  • Add to Cart
    98168p-mCherry-C1-iC++_CASynthetic construct iC++_C128A gene (Synthetic)Mammalian ExpressionArtificial anion-conducting channelrhodopsins with tuned spectra, modified kinetics and enhanced light sensitivity biorxiv slow cycling (open for minutes) step-function artificial anion conducting channelrhodopsin (aACR). High light sensitivity. Activation with cyan light, inactivation max with 590 nm (accelarates closure to ms). Codon optimized for mammalian expression. pEGFP-C1
  • Tag / Fusion Protein
    • mCherry (C terminal on backbone)
  • Add to Cart
    98169p-mCherry-C1-Phobos_CASynthetic construct Phobos_C128A gene (Synthetic)Mammalian ExpressionArtificial anion-conducting channelrhodopsins with tuned spectra, modified kinetics and enhanced light sensitivity biorxiv slow cycling (open for minutes) step-function artificial anion conducting channelrhodopsin (aACR). High light sensitivity. Activation with blue light, inactivation max with 580 nm (accelarates closure to ms). Codon optimized for mammalian expression. pEGFP-C1
  • Tag / Fusion Protein
    • mCherry (C terminal on backbone)
  • Add to Cart
    98170p-mCherry-C1-Aurora_CASynthetic construct Aurora_C128A gene (Synthetic)Mammalian ExpressionArtificial anion-conducting channelrhodopsins with tuned spectra, modified kinetics and enhanced light sensitivity biorxiv slow cycling (open for minutes) step-function artificial anion conducting channelrhodopsin (aACR). High light sensitivity. Activation with green light, inactivation max with 625 nm (accelarates closure to ms). Not recommended for in vivo use. Codon optimized for mammalian expression. pEGFP-C1
  • Tag / Fusion Protein
    • mCherry (C terminal on backbone)
  • Add to Cart
    98171p-mCherry-N1-iChloC_CASynthetic construct iChloC_C128A gene (Synthetic)Mammalian ExpressionArtificial anion-conducting channelrhodopsins with tuned spectra, modified kinetics and enhanced light sensitivity biorxiv slow cycling (open for minutes) step-function artificial anion conducting channelrhodopsin (aACR). High light sensitivity. Activation with blue to green light, inactivation max with 605 nm (accelarates closure to ms). Codon optimized for mammalian expression. pEGFP-N1
  • Tag / Fusion Protein
    • mCherry (C terminal on backbone)
  • Add to Cart
    98172p-mCherry-C1-iChR2++Synthetic construct iChR2++ gene (Synthetic)Mammalian ExpressionArtificial anion-conducting channelrhodopsins with tuned spectra, modified kinetics and enhanced light sensitivity biorxiv artificial anion conducting channelrhodopsin (aACR). Activation with blue light. Codon optimized for mammalian expression. pEGFP-C1
  • Tag / Fusion Protein
    • mCherry (C terminal on backbone)
  • Add to Cart
    98173p-mCherry-C1-iCoChR++Synthetic construct iCoChR++ gene (Synthetic)Mammalian ExpressionArtificial anion-conducting channelrhodopsins with tuned spectra, modified kinetics and enhanced light sensitivity biorxiv artificial anion conducting channelrhodopsin (aACR). Activation with blue light. Codon optimized for mammalian expression. pEGFP-C1
  • Tag / Fusion Protein
    • mCherry (C terminal on backbone)
  • Add to Cart