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Qi-Jun Chen Lab Plasmids


The Qi-Jun Chen Lab has deposited plasmids at Addgene for distribution to the research community. Addgene is a nonprofit plasmid repository dedicated to improving life science research.

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Title Authors Publication
A CRISPR/Cas9 toolkit for multiplex genome editing in plants. Xing HL, Dong L, Wang ZP, Zhang HY, Han CY, Liu B, Wang XC, Chen QJ BMC Plant Biol. 2014 Nov 29;14(1):327.
Egg cell-specific promoter-controlled CRISPR/Cas9 efficiently generates homozygous mutants for multiple target genes in Arabidopsis in a single generation. Wang ZP, Xing HL, Dong L, Zhang HY, Han CY, Wang XC, Chen QJ Genome Biol. 2015 Jul 21;16:144. doi: 10.1186/s13059-015-0715-0.
CRISPR/Cas9-mediated base-editing system efficiently generates gain-of-function mutations in Arabidopsis. Chen Y, Wang Z, Ni H, Xu Y, Chen Q, Jiang L Sci China Life Sci. 2017 Mar 16. doi: 10.1007/s11427-017-9021-5.
ID Plasmid Gene/Insert Vector Type Publication Hidden Extra Search Info  
50579pBUN6A11dCas9-VP64 (Synthetic), gRNA scaffold (Synthetic)CRISPR ; Plant expressionA CRISPR/Cas9 toolkit for multiplex genome editing in plants. BMC Plant Biol. 2014 Nov 29;14(1):327. CRISPR/Cas based plant genome editing and gene regulation; expresses dCas9-VP64, gRNA scaffold for insertion of target sequence (OsU3 promoter), Bar resistance pGreen-like binary vector Add to Cart
50580pBUN6I11dCas9-KRAB (Synthetic), gRNA scaffold (Synthetic)CRISPR ; Plant expressionA CRISPR/Cas9 toolkit for multiplex genome editing in plants. BMC Plant Biol. 2014 Nov 29;14(1):327. CRISPR/Cas based plant genome editing and gene regulation; expresses dCas9-KRAB, gRNA scaffold for insertion of target sequence (OsU3 promoter), Bar resistance pGreen-like binary vector Add to Cart
50581pBUN4113×FLAG-NLS-zCas9-NLS (Synthetic), gRNA scaffold (Synthetic)CRISPR ; Plant expressionA CRISPR/Cas9 toolkit for multiplex genome editing in plants. BMC Plant Biol. 2014 Nov 29;14(1):327. CRISPR/Cas based plant genome editing and gene regulation; expresses 3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence (OsU3 promoter), Bar resistance pGreen-like binary vector Add to Cart
50582pBUN501zCas9D10A (Synthetic), gRNA scaffold (Synthetic)CRISPR ; Plant expressionA CRISPR/Cas9 toolkit for multiplex genome editing in plants. BMC Plant Biol. 2014 Nov 29;14(1):327. CRISPR/Cas based plant genome editing and gene regulation; expresses zCas9D10A, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Bar resistance pGreen-like binary vector Add to Cart
50586pHSN6A01dCas9-VP64 (Synthetic), gRNA scaffold (Synthetic)CRISPR ; Plant expressionA CRISPR/Cas9 toolkit for multiplex genome editing in plants. BMC Plant Biol. 2014 Nov 29;14(1):327. CRISPR/Cas based plant genome editing and gene regulation; expresses dCas9-VP64, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Hyg resistance pGreen-like binary vector Add to Cart
50587pHSN6I01dCas9-KRAB (Synthetic), gRNA scaffold (Synthetic)CRISPR ; Plant expressionA CRISPR/Cas9 toolkit for multiplex genome editing in plants. BMC Plant Biol. 2014 Nov 29;14(1):327. CRISPR/Cas based plant genome editing and gene regulation; expresses dCas9-KRAB, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Hyg resistance pGreen-like binary vector Add to Cart
50588pHSN4013×FLAG-NLS-zCas9-NLS (Synthetic), gRNA scaffold (Synthetic)CRISPR ; Plant expressionA CRISPR/Cas9 toolkit for multiplex genome editing in plants. BMC Plant Biol. 2014 Nov 29;14(1):327. CRISPR/Cas based plant genome editing and gene regulation; expresses 3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Hyg resistance pGreen-like binary vector Add to Cart
50589pHSN501zCas9D10A (Synthetic), gRNA scaffold (Synthetic)CRISPR ; Plant expressionA CRISPR/Cas9 toolkit for multiplex genome editing in plants. BMC Plant Biol. 2014 Nov 29;14(1):327. CRISPR/Cas based plant genome editing and gene regulation; expresses zCas9D10A, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Hyg resistance pGreen-like binary vector Add to Cart
50590pCBC-DT1T2T1, U626t plus, U629p, T2 (Synthetic)Plant Expression, CRISPR ; PCR templateA CRISPR/Cas9 toolkit for multiplex genome editing in plants. BMC Plant Biol. 2014 Nov 29;14(1):327. CRISPR/Cas based plant genome editing and gene regulation; used as template for making expression cassette with multiple gRNA target sites pGWC Add to Cart
50591pCBC-DT2T3T2, gRNA scaffold, U6-29t plus, U6-1p, T3 (Synthetic)Plant Expression, CRISPR ; PCR templateA CRISPR/Cas9 toolkit for multiplex genome editing in plants. BMC Plant Biol. 2014 Nov 29;14(1):327. CRISPR/Cas based plant genome editing and gene regulation; used as template for making expression cassette with multiple gRNA target sites pGWC Add to Cart
50592pCBC-DT3T4T3, gRNA scaffold, U6-1tplus, U6-26p, T4 (Synthetic)Plant Expression, CRISPR ; PCR templateA CRISPR/Cas9 toolkit for multiplex genome editing in plants. BMC Plant Biol. 2014 Nov 29;14(1):327. CRISPR/Cas based plant genome editing and gene regulation; used as template for making expression cassette with multiple gRNA target sites pGWC Add to Cart
50593pCBC-MT1T2T1, gRNA scaffold, OsU3t plus, TaU3p, T2 (Synthetic)Plant Expression, CRISPR ; PCR templateA CRISPR/Cas9 toolkit for multiplex genome editing in plants. BMC Plant Biol. 2014 Nov 29;14(1):327. CRISPR/Cas based plant genome editing and gene regulation; used as template for making expression cassette with multiple gRNA target sites pGWC Add to Cart
50594pCBC-MT2T3T2, gRNA scaffold, TaU3t plus, U6-26p, T3 (Synthetic)Plant Expression, CRISPR ; PCR templateA CRISPR/Cas9 toolkit for multiplex genome editing in plants. BMC Plant Biol. 2014 Nov 29;14(1):327. CRISPR/Cas based plant genome editing and gene regulation; used as template for making expression cassette with multiple gRNA target sites pGWC Add to Cart
50595pCBC-MT3T4T3, gRNA scaffold, U6-26t plus, OsU3p, T4 (Synthetic)Plant Expression, CRISPR ; PCR templateA CRISPR/Cas9 toolkit for multiplex genome editing in plants. BMC Plant Biol. 2014 Nov 29;14(1):327. CRISPR/Cas based plant genome editing and gene regulation; used as template for making expression cassette with multiple gRNA target sites pGWC Add to Cart
62200pBUE411gRNA scaffold (Synthetic), zCas9 (Other)Plant Expression ; plant binary vectorA CRISPR/Cas9 toolkit for multiplex genome editing in plants. BMC Plant Biol. 2014 Nov 29;14(1):327. CRISPR/Cas based plant genome editing and gene regulation; expresses 3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence (OsU3 promoter), Bar resistance pCambia Add to Cart
62201pHSE401gRNA scaffold (Synthetic), zCas9 (Other)Plant Expression ; plant binary vectorA CRISPR/Cas9 toolkit for multiplex genome editing in plants. BMC Plant Biol. 2014 Nov 29;14(1):327. CRISPR/Cas based plant genome editing and gene regulation; expresses 3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Hyg resistance pCambia Add to Cart
62202pKSE401gRNA scaffold (Synthetic), zCas9 (Other)Plant Expression ; plant binary vectorA CRISPR/Cas9 toolkit for multiplex genome editing in plants. BMC Plant Biol. 2014 Nov 29;14(1):327. CRISPR/Cas based plant genome editing and gene regulation; expresses 3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Kan resistance pCambia Add to Cart
62203pHUE411gRNA scaffold (Synthetic), zCas9 (Other)Plant Expression ; plant binary vectorA CRISPR/Cas9 toolkit for multiplex genome editing in plants. BMC Plant Biol. 2014 Nov 29;14(1):327. CRISPR/Cas based plant genome editing and gene regulation; expresses 3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence (OsU3 promoter), Hyg resistance pCambia Add to Cart
62204pBUN421gRNA scaffold (Synthetic), zCas9 (Other)Plant Expression ; plant binary vectorA CRISPR/Cas9 toolkit for multiplex genome editing in plants. BMC Plant Biol. 2014 Nov 29;14(1):327. CRISPR/Cas based plant genome editing and gene regulation; expresses 3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence (TaU3 promoter), Bar resistance pGreen-like Add to Cart
71286pHEE401sgRNA scaffold (Synthetic), zCas9 (Other)Plant Expression, CRISPR ; Plant binary vectorEgg cell-specific promoter-controlled CRISPR/Cas9 efficiently generates homozygous mutants for multiple target genes in Arabidopsis in a single generation. Genome Biol. 2015 Jul 21;16:144. doi: 10.1186/s13059-015-0715-0. Egg cell-specific promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 promoter), Hyg resistance pCambia Add to Cart
71287pHEE401EgRNA scaffold (Synthetic), zCas9 (Other)Plant Expression, CRISPR ; Plant binary vectorEgg cell-specific promoter-controlled CRISPR/Cas9 efficiently generates homozygous mutants for multiple target genes in Arabidopsis in a single generation. Genome Biol. 2015 Jul 21;16:144. doi: 10.1186/s13059-015-0715-0. Egg cell-specific promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 promoter), Hyg resistance pCambia Add to Cart
71288pHEE2E-TRIsgRNA targeting TRY and CPC genes (Synthetic), sgRNA targeting ETC2 gene (Synthetic), zCas9 (Other)Plant Expression, CRISPR ; Plant binary vectorEgg cell-specific promoter-controlled CRISPR/Cas9 efficiently generates homozygous mutants for multiple target genes in Arabidopsis in a single generation. Genome Biol. 2015 Jul 21;16:144. doi: 10.1186/s13059-015-0715-0. Egg cell-specific promoter-controlled expression 3×FLAG-NLS-zCas9-NLS and two sgRNAs targeting three genes: ETC2, TRY, and CPC pCambia Add to Cart
91707pHEE901Plant ExpressionCRISPR/Cas9-mediated base-editing system efficiently generates gain-of-function mutations in Arabidopsis. Sci China Life Sci. 2017 Mar 16. doi: 10.1007/s11427-017-9021-5. CRISPR/Cas9-mediated base editing in Arabidopsis. Contains Cas9(D10A) and empty gRNA scaffold, HygR pCambia Add to Cart
91708pHSE901Plant ExpressionCRISPR/Cas9-mediated base-editing system efficiently generates gain-of-function mutations in Arabidopsis. Sci China Life Sci. 2017 Mar 16. doi: 10.1007/s11427-017-9021-5. CRISPR/Cas9-mediated base editing in dicots. Contains Cas9(D10A) under CaMV 35S promoter and empty gRNA scaffold, HygR pCambia Add to Cart
91709pBSE901Plant ExpressionCRISPR/Cas9-mediated base-editing system efficiently generates gain-of-function mutations in Arabidopsis. Sci China Life Sci. 2017 Mar 16. doi: 10.1007/s11427-017-9021-5. CRISPR/Cas9-mediated base editing in dicots. Contains Cas9(D10A) under CaMV 35S promoter and empty gRNA scaffold, Basta resistance pCambia Add to Cart
91713pXSE901BPlant ExpressionCRISPR/Cas9-mediated base-editing system efficiently generates gain-of-function mutations in Arabidopsis. Sci China Life Sci. 2017 Mar 16. doi: 10.1007/s11427-017-9021-5. CRISPR/Cas9-mediated base editing in dicots. Contains Cas9(D10A) under CaMV 35S promoter and empty gRNA scaffold, Bar resistance pCambia Add to Cart
91714pXSE901BGPlant ExpressionCRISPR/Cas9-mediated base-editing system efficiently generates gain-of-function mutations in Arabidopsis. Sci China Life Sci. 2017 Mar 16. doi: 10.1007/s11427-017-9021-5. CRISPR/Cas9-mediated base editing in dicots. Contains Cas9(D10A) under CaMV 35S promoter and empty gRNA scaffold, Basta and glyphosate-resistance pCambia Add to Cart
91715pKEE401Plant ExpressionEgg cell-specific promoter-controlled CRISPR/Cas9 efficiently generates homozygous mutants for multiple target genes in Arabidopsis in a single generation. Genome Biol. 2015 Jul 21;16:144. doi: 10.1186/s13059-015-0715-0. CRISPR/Cas9-mediated genome editing in Arabidopsis. Contains Cas9 and empty gRNA scaffold, Kan resistance pCambia Add to Cart
91716pXEE401BPlant ExpressionEgg cell-specific promoter-controlled CRISPR/Cas9 efficiently generates homozygous mutants for multiple target genes in Arabidopsis in a single generation. Genome Biol. 2015 Jul 21;16:144. doi: 10.1186/s13059-015-0715-0. CRISPR/Cas9-mediated genome editing in Arabidopsis. Contains Cas9 and empty gRNA scaffold, Bar resistance pCambia Add to Cart
91717pXEE401BGPlant ExpressionEgg cell-specific promoter-controlled CRISPR/Cas9 efficiently generates homozygous mutants for multiple target genes in Arabidopsis in a single generation. Genome Biol. 2015 Jul 21;16:144. doi: 10.1186/s13059-015-0715-0. CRISPR/Cas9-mediated genome editing in Arabidopsis, Bar and glyphosate-resistance pCambia Add to Cart