Rachel Klevit Lab Plasmids


The Rachel Klevit Lab has deposited plasmids at Addgene for distribution to the research community. Addgene is a non-profit plasmid repository dedicated to improving life science research.

Learn more about research in the Rachel Klevit Lab.

Addgene Alerts

You must login or create an account to subscribe for Addgene Alerts.

Title Authors Publication
UBCH7 reactivity profile reveals parkin and HHARI to be RING/HECT hybrids. Wenzel DM, Lissounov A, Brzovic PS, Klevit RE Nature. 2011 May 1. ():.
E2-BRCA1 RING interactions dictate synthesis of mono- or specific polyubiquitin chain linkages. Christensen DE, Brzovic PS, Klevit RE Nat Struct Mol Biol. 2007 Oct . 14(10):941-8.
A UbcH5/ubiquitin noncovalent complex is required for processive BRCA1-directed ubiquitination. Brzovic PS, Lissounov A, Christensen DE, Hoyt DW, Klevit RE Mol Cell. 2006 Mar 17. 21(6):873-80.
ID Plasmid Gene/Insert Vector Type Publication Hidden Extra Search Info  
12643 UbcH5c WT pET28a UbcH5c - Ubiquitin conjugating enzyme (Homo sapiens) Bacterial Expression Brzovic et al Mol Cell. 2006 Mar 17. 21(6):873-80. pET28a UBE2D3 E2(17)KB3, UBC4/5, UBCH5C
12644 UbcH5c S22R pET28a Ubch5c - Ubiquitin conjugating enzyme (Homo sapiens) Bacterial Expression Brzovic et al Mol Cell. 2006 Mar 17. 21(6):873-80. pET28a UBE2D3 E2(17)KB3, UBC4/5, UBCH5C
12645 BRCA1 (1-304) E3-Ring domain, Ub-ligase (Homo sapiens) Bacterial Expression Brzovic et al Mol Cell. 2006 Mar 17. 21(6):873-80. pET151D topo BRCA1 BRCAI, BRCC1, BROVCA1, IRIS, PNCA4, PPP1R53, PSCP, RNF53
12646 BARD1 26-327 pET28a BRCA1 binding partner for a Ring domain E3 Ub-ligase (Homo sapiens) Bacterial Expression Brzovic et al Mol Cell. 2006 Mar 17. 21(6):873-80. pET28a BARD1
12647 Ubiquitin WT Ubiquitin - wild type (Homo sapiens) Bacterial Expression Brzovic et al Mol Cell. 2006 Mar 17. 21(6):873-80. pET15 UBC HMG20
12648 Ubiquitin I44A Ubiquitin (Homo sapiens) Bacterial Expression Brzovic et al Mol Cell. 2006 Mar 17. 21(6):873-80. pET15
31254 pGEX-4T HHARI RBR Human Homologue of Ariadne (HHARI) (Homo sapiens) Bacterial Expression Wenzel et al Nature. 2011 May 1. ():. pGEX-4T2 ARIH1 HUSSY-27, ARI, HARI, HHARI, UBCH7BP
31255 pGEX-4T2 Parkin RBR Parkin (Rattus norvegicus) Bacterial Expression Wenzel et al Nature. 2011 May 1. ():. pGEX-4T2 Park2 Park, Prkn
31407 Ube2g1 Ubiquitin Conjugating Enzyme (Homo sapiens) Yeast Expression Christensen et al Nat Struct Mol Biol. 2007 Oct . 14(10):941-8. pACT2 UBE2G1 E217K, UBC7, UBE2G
31408 Ube2b Ubiquitin Conjugating Enzyme (Homo sapiens) Yeast Expression Christensen et al Nat Struct Mol Biol. 2007 Oct . 14(10):941-8. pACT2 UBE2B E2-17kDa, HHR6B, HR6B, RAD6B, UBC2
31409 Ube2d1 Ubiquitin Conjugating Enzyme (Homo sapiens) Yeast Expression Christensen et al Nat Struct Mol Biol. 2007 Oct . 14(10):941-8. pACT2 UBE2D1 E2(17)KB1, SFT, UBC4/5, UBCH5, UBCH5A
31410 Ube2d2 Ubiquitin Conjugating Enzyme (Homo sapiens) Yeast Expression Christensen et al Nat Struct Mol Biol. 2007 Oct . 14(10):941-8. pACT2 UBE2D2 E2(17)KB2, PUBC1, UBC4, UBC4/5, UBCH4, UBCH5B
31411 Ube2d3 Ubiquitin Conjugating Enzyme (Homo sapiens) Yeast Expression Christensen et al Nat Struct Mol Biol. 2007 Oct . 14(10):941-8. pACT2 UBE2D3 E2(17)KB3, UBC4/5, UBCH5C
31412 TSG101 Tumor susceptibility gene 101 (Homo sapiens) Yeast Expression Christensen et al Nat Struct Mol Biol. 2007 Oct . 14(10):941-8. pACT2 TSG101 TSG10, VPS23
31413 Ube2e1 Ubiquitin Conjugating Enzyme (Homo sapiens) Yeast Expression Christensen et al Nat Struct Mol Biol. 2007 Oct . 14(10):941-8. pACT2 UBE2E1 UBCH6
31414 Ube2e2 Ubiquitin Conjugating Enzyme (Homo sapiens) Yeast Expression Christensen et al Nat Struct Mol Biol. 2007 Oct . 14(10):941-8. pACT2 UBE2E2 UBCH8
31415 Ube2e3 Ubiquitin Conjugating Enzyme (Homo sapiens) Yeast Expression Christensen et al Nat Struct Mol Biol. 2007 Oct . 14(10):941-8. pACT2
31416 Ube2f Ubiquitin Conjugating Enzyme (Homo sapiens) Yeast Expression Christensen et al Nat Struct Mol Biol. 2007 Oct . 14(10):941-8. pACT2 UBE2F MGC18120, NCE2
31417 Ube2s Ubiquitin Conjugating Enzyme (Homo sapiens) Yeast Expression Christensen et al Nat Struct Mol Biol. 2007 Oct . 14(10):941-8. pACT2
31418 Ube2c Ubiquitin Conjugating Enzyme (Homo sapiens) Yeast Expression Christensen et al Nat Struct Mol Biol. 2007 Oct . 14(10):941-8. pACT2 UBE2C RP3-447F3.1, UBCH10, dJ447F3.2
31419 Ube2h Ubiquitin Conjugating Enzyme (Homo sapiens) Yeast Expression Christensen et al Nat Struct Mol Biol. 2007 Oct . 14(10):941-8. pACT2 UBE2H E2-20K, GID3, UBC8, UBCH, UBCH2
31420 Ube2i Ubiquitin Conjugating Enzyme (Homo sapiens) Yeast Expression Christensen et al Nat Struct Mol Biol. 2007 Oct . 14(10):941-8. pACT2 UBE2I LA16c-358B7.1, C358B7.1, P18, UBC9
31421 Ube2L3 Ubiquitin Conjugating Enzyme (Homo sapiens) Yeast Expression Christensen et al Nat Struct Mol Biol. 2007 Oct . 14(10):941-8. pACT2 UBE2L3 E2-F1, L-UBC, UBCH7, UbcM4
31422 Ube2L6 Ubiquitin Conjugating Enzyme (Homo sapiens) Yeast Expression Christensen et al Nat Struct Mol Biol. 2007 Oct . 14(10):941-8. pACT2 UBE2L6 MGC40331, RIG-B, UBCH8
31423 Ube2m Ubiquitin Conjugating Enzyme (Homo sapiens) Yeast Expression Christensen et al Nat Struct Mol Biol. 2007 Oct . 14(10):941-8. pACT2 UBE2M UBC-RS2, UBC12, hUbc12
31425 Ube2q2 Ubiquitin Conjugating Enzyme (Homo sapiens) Yeast Expression Christensen et al Nat Struct Mol Biol. 2007 Oct . 14(10):941-8. pACT2 UBE2Q2 DKFZp762C143
31426 Ube2r1 Ubiquitin Conjugating Enzyme (Homo sapiens) Yeast Expression Christensen et al Nat Struct Mol Biol. 2007 Oct . 14(10):941-8. pACT2 CDC34 E2-CDC34, UBC3, UBCH3, UBE2R1
31427 Ube2t Ubiquitin Conjugating Enzyme (Homo sapiens) Yeast Expression Christensen et al Nat Struct Mol Biol. 2007 Oct . 14(10):941-8. pACT2 UBE2T HSPC150, PIG50
31428 Ube2u Ubiquitin Conjugating Enzyme (Homo sapiens) Yeast Expression Christensen et al Nat Struct Mol Biol. 2007 Oct . 14(10):941-8. pACT2 UBE2U RP4-636O23.1
31429 Ube2v1 Ubiquitin Conjugating Enzyme (Homo sapiens) Yeast Expression Christensen et al Nat Struct Mol Biol. 2007 Oct . 14(10):941-8. pACT2 UBE2V1 P/OKcl.19, CIR1, CROC-1, CROC1, UBE2V, UEV-1, UEV1, UEV1A
31430 Ube2v2 Ubiquitin Conjugating Enzyme (Homo sapiens) Yeast Expression Christensen et al Nat Struct Mol Biol. 2007 Oct . 14(10):941-8. pACT2 UBE2V2 DDVIT1, DDVit-1, EDAF-1, EDPF-1, EDPF1, MMS2, UEV-2, UEV2
31431 Ube2v3 Ubiquitin Conjugating Enzyme (Homo sapiens) Yeast Expression Christensen et al Nat Struct Mol Biol. 2007 Oct . 14(10):941-8. pACT2 UEVLD ATTP, UEV3
31432 Ube2w Ubiquitin Conjugating Enzyme (Homo sapiens) Yeast Expression Christensen et al Nat Struct Mol Biol. 2007 Oct . 14(10):941-8. pACT2 UBE2W UBC-16, UBC16
31435 FT1 Fused Toes Homolog (Homo sapiens) Yeast Expression Christensen et al Nat Struct Mol Biol. 2007 Oct . 14(10):941-8. pACT2 AKTIP FT1, FTS
31436 HIP2 Huntingtin Interacting protein 2 (Homo sapiens) Yeast Expression Christensen et al Nat Struct Mol Biol. 2007 Oct . 14(10):941-8. pACT2 UBE2K E2-25K, HIP2, HYPG, LIG, UBC1