Rick Horwitz Lab Plasmids


The Rick Horwitz Lab has deposited plasmids at Addgene for distribution to the research community. Addgene is a non-profit plasmid repository dedicated to improving life science research.

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Title Authors Publication
Identification of protein networks associated with the PAK1-betaPIX-GIT1-paxillin signaling complex by mass spectrometry. Mayhew MW, Webb DJ, Kovalenko M, Whitmore L, Fox JW, Horwitz AF J Proteome Res. 2006 Sep . 5(9):2417-23.
Identification of phosphorylation sites in GIT1. Webb DJ, Mayhew MW, Kovalenko M, Schroeder MJ, Jeffery ED, Whitmore L, Shabanowitz J, Hunt DF, Horwitz AF J Cell Sci. 2006 Jul 15. 119(Pt 14):2847-50.
Paxillin phosphorylation sites mapped by mass spectrometry. Webb DJ, Schroeder MJ, Brame CJ, Whitmore L, Shabanowitz J, Hunt DF, Horwitz AR J Cell Sci. 2005 Nov 1. 118(Pt 21):4925-9.
GIT1 functions in a motile, multi-molecular signaling complex that regulates protrusive activity and cell migration. Manabe R, Kovalenko M, Webb DJ, Horwitz AR J Cell Sci. 2002 Apr 1. 115(Pt 7):1497-510.
Single-molecule speckle analysis of actin filament turnover in lamellipodia. Watanabe N, Mitchison TJ Science. 2002 Feb 8. 295(5557):1083-6.
Differential dynamics of alpha 5 integrin, paxillin, and alpha-actinin during formation and disassembly of adhesions in migrating cells. Laukaitis CM, Webb DJ, Donais K, Horwitz AF J Cell Biol. 2001 Jun 25. 153(7):1427-40.
Horwitz lab unpublished plasmids (unpublished)
ID Plasmid Gene/Insert Vector Type Publication Hidden Extra Search Info  
15211 Flag-ECFP-Paxillin paxillin (Gallus gallus) Mammalian Expression Webb et al J Cell Sci. 2005 Nov 1. 118(Pt 21):4925-9. pcDNA3 PXN
15212 Flag-Paxillin Paxillin (Gallus gallus) Mammalian Expression Webb et al J Cell Sci. 2005 Nov 1. 118(Pt 21):4925-9. pcDNA3 PXN
15223 Flag-ECFP-GIT1 G protein coupled receptor kinase-interacting protein 1 (Homo sapiens) Mammalian Expression Webb et al J Cell Sci. 2006 Jul 15. 119(Pt 14):2847-50. pCDNA3 GIT1
15225 GIT1A-FLAG G protein coupled receptor kinase-interacting protein 1 (Homo sapiens) Mammalian Expression Manabe et al J Cell Sci. 2002 Apr 1. 115(Pt 7):1497-510. pEGFP-N1 GIT1
15226 pEGFP-GIT1 G protein coupled receptor kinase-interacting protein 1 (Homo sapiens) Mammalian Expression Manabe et al J Cell Sci. 2002 Apr 1. 115(Pt 7):1497-510. PEGFP-C1 GIT1
15233 Paxillin-pEGFP paxillin (Gallus gallus) Mammalian Expression Laukaitis et al J Cell Biol. 2001 Jun 25. 153(7):1427-40. pEGFP-N3 PXN
15234 Flag-betaPIXa beta-PIXa (Mus musculus) Mammalian Expression Mayhew et al J Proteome Res. 2006 Sep . 5(9):2417-23. pcDNA3 Arhgef7 PIX, Cool, Pak3bp, cool-1, p85SPR, betaPix, p85Cool1, betaPix-b, betaPix-c, mKIAA0142
15235 Flag-ECFP-betaPIXa beta-PIXa (Mus musculus) Mammalian Expression Mayhew et al J Proteome Res. 2006 Sep . 5(9):2417-23. pcDNA3 Arhgef7 PIX, Cool, Pak3bp, cool-1, p85SPR, betaPix, p85Cool1, betaPix-b, betaPix-c, mKIAA0142
15238 Alpha 5 integrin-GFP alpha 5 integrin (Homo sapiens) Mammalian Expression Laukaitis et al J Cell Biol. 2001 Jun 25. 153(7):1427-40. pEGFP-N3 ITGA5 CD49e, FNRA, VLA5A
30450 Reduced Expression Alpha-5-Integrin-GFP alpha 5 integrin (Homo sapiens) Mammalian Expression Horwitz lab unpublished plasmids (unpublished) pEGFP-N3 ITGA5 CD49e, FNRA, VLA5A
31502 Reduced Expression GFP beta actin beta actin (Homo sapiens) Mammalian Expression Watanabe et al Science. 2002 Feb 8. 295(5557):1083-6. pEGFP-C1 ACTB BRWS1, PS1TP5BP1