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Robert Campbell Lab Plasmids


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Title Authors Publication
Exploration of New Chromophore Structures Leads to the Identification of Improved Blue Fluorescent Proteins. Ai HW, Shaner NC, Cheng Z, Tsien RY, Campbell RE Biochemistry. 2007 Apr 20. ():.
Fluorescent protein FRET pairs for ratiometric imaging of dual biosensors. Ai HW, Hazelwood KL, Davidson MW, Campbell RE Nat Methods. 2008 May . 5(5):401-3.
Red fluorescent protein pH biosensor to detect concentrative nucleoside transport. Johnson DE, Ai HW, Wong P, Young JD, Campbell RE, Casey J J Biol Chem. 2009 Jun 3. ():.
Circularly permuted monomeric red fluorescent proteins with new termini in the beta-sheet. Carlson HJ, Cotton DW, Campbell RE Protein Sci. 2010 Jun 2. ():.
An Expanded Palette of Genetically Encoded Ca2+ Indicators. Zhao Y, Araki S, Wu J, Teramoto T, Chang YF, Nakano M, Abdelfattah AS, Fujiwara M, Ishihara T, Nagai T, Campbell RE Science. 2011 Sep 8.
Forster resonance energy transfer-based biosensors for multiparameter ratiometric imaging of Ca2+ dynamics and caspase-3 activity in single cells. Ding Y, Ai HW, Hoi H, Campbell RE Anal Chem. 2011 Dec 15;83(24):9687-93. doi: 10.1021/ac202595g. Epub 2011 Nov 28.
A fluorogenic red fluorescent protein heterodimer. Alford SC, Abdelfattah AS, Ding Y, Campbell RE Chem Biol. 2012 Mar 23;19(3):353-60.
Dimerization-dependent green and yellow fluorescent proteins. Alford SC, Ding Y, Simmen T, Campbell RE ACS Synth Biol. 2012 Dec 21;1(12):569-75. doi: 10.1021/sb300050j. Epub 2012 Aug 14.
Highlightable Ca(2+) Indicators for Live Cell Imaging. Hoi H, Matsuda T, Nagai T, Campbell RE J Am Chem Soc. 2012 Dec 26.
Improved Orange and Red Ca Indicators and Photophysical Considerations for Optogenetic Applications. Wu J, Liu L, Matsuda T, Zhao Y, Rebane A, Drobizhev M, Chang YF, Araki S, Arai Y, March K, Hughes TE, Sagou K, Miyata T, Nagai T, Li WH, Campbell RE ACS Chem Neurosci. 2013 Mar 19.
An engineered monomeric Zoanthus sp. yellow fluorescent protein. Hoi H, Howe ES, Ding Y, Zhang W, Baird MA, Sell BR, Allen JR, Davidson MW, Campbell RE Chem Biol. 2013 Oct 24;20(10):1296-304. doi: 10.1016/j.chembiol.2013.08.008. Epub 2013 Oct 3.
Circular permutated red fluorescent proteins and calcium ion indicators based on mCherry. Carlson HJ, Campbell RE Protein Eng Des Sel. 2013 Dec;26(12):763-72. doi: 10.1093/protein/gzt052. Epub 2013 Oct 22.
Microfluidic cell sorter-aided directed evolution of a protein-based calcium ion indicator with an inverted fluorescent response. Zhao Y, Abdelfattah AS, Zhao Y, Ruangkittisakul A, Ballanyi K, Campbell RE, Harrison DJ Integr Biol (Camb). 2014 May 19.
Michael Davidson Empty Backbones (unpublished)
pHuji, a pH-sensitive red fluorescent protein for imaging of exo- and endocytosis. Shen Y, Rosendale M, Campbell RE, Perrais D J Cell Biol. 2014 Nov 10;207(3):419-32. doi: 10.1083/jcb.201404107.
Ratiometric biosensors based on dimerization-dependent fluorescent protein exchange. Ding Y, Li J, Enterina JR, Shen Y, Zhang I, Tewson PH, Mo GC, Zhang J, Quinn AM, Hughes TE, Maysinger D, Alford SC, Zhang Y, Campbell RE Nat Methods. 2015 Jan 26. doi: 10.1038/nmeth.3261.
Red fluorescent genetically encoded Ca2+ indicators for use in mitochondria and endoplasmic reticulum. Wu J, Prole DL, Shen Y, Lin Z, Gnanasekaran A, Liu Y, Chen L, Zhou H, Chen SR, Usachev YM, Taylor CW, Campbell RE Biochem J. 2014 Nov 15;464(1):13-22. doi: 10.1042/BJ20140931.
A long Stokes shift red fluorescent Ca(2+) indicator protein for two-photon and ratiometric imaging. Wu J, Abdelfattah AS, Miraucourt LS, Kutsarova E, Ruangkittisakul A, Zhou H, Ballanyi K, Wicks G, Drobizhev M, Rebane A, Ruthazer ES, Campbell RE Nat Commun. 2014 Oct 31;5:5262. doi: 10.1038/ncomms6262.
A Bright and Fast Red Fluorescent Protein Voltage Indicator That Reports Neuronal Activity in Organotypic Brain Slices. Abdelfattah AS, Farhi SL, Zhao Y, Brinks D, Zou P, Ruangkittisakul A, Platisa J, Pieribone VA, Ballanyi K, Cohen AE, Campbell RE J Neurosci. 2016 Feb 24;36(8):2458-72. doi: 10.1523/JNEUROSCI.3484-15.2016.
ID Plasmid Gene/Insert Vector Type Publication Hidden Extra Search Info  
14891pBad-EBFP2EBFP2Bacterial ExpressionExploration of New Chromophore Structures Leads to the Identification of Improved Blue Fluorescent Proteins. Biochemistry. 2007 Apr 20. ():. Bacterial expression of EBFP2 blue fluorescent protein pBad/His B Add to Cart
14892pBad-mKalama1mKalama1Bacterial ExpressionExploration of New Chromophore Structures Leads to the Identification of Improved Blue Fluorescent Proteins. Biochemistry. 2007 Apr 20. ():. Bacterial expression of mKalama1 blue fluorescent protein pBad/His B Add to Cart
14893pEBFP2-NucpEBFP2-NucMammalian ExpressionExploration of New Chromophore Structures Leads to the Identification of Improved Blue Fluorescent Proteins. Biochemistry. 2007 Apr 20. ():. Mammalian expression of EBFP2 blue fluorescent protein with NLS pEYFP-Nuc Add to Cart
14894pmKalama1-NucpmKalama1-Nuc (Other)Mammalian ExpressionExploration of New Chromophore Structures Leads to the Identification of Improved Blue Fluorescent Proteins. Biochemistry. 2007 Apr 20. ():. Mammalian expression of mKalama1 blue fluorescent protein with NLS pEYFP-Nuc Add to Cart
18083pBad-mAmetrinemAmetrine fluorescent proteinsBacterial ExpressionFluorescent protein FRET pairs for ratiometric imaging of dual biosensors. Nat Methods. 2008 May . 5(5):401-3. Bacterial expression of mAmetrine fluorescent protein pBad/His B Add to Cart
18084pBad-mAmetrine1.1mAmetrine1.1Bacterial ExpressionFluorescent protein FRET pairs for ratiometric imaging of dual biosensors. Nat Methods. 2008 May . 5(5):401-3. Bacterial expression of mAmetrine (version 1.1) fluorescent protein pBad/His B Add to Cart
18879pmAmetrine-DEVD-tdTomatomAmetrine-DEVD-tdTomato (Homo sapiens)Mammalian ExpressionFluorescent protein FRET pairs for ratiometric imaging of dual biosensors. Nat Methods. 2008 May . 5(5):401-3. Caspase-3 FRET biosensor, mammalian expression of mAmetrine-DEVD-tdTomato pEYFP-Nuc CASP3 CPP32, CPP32B, SCA-1 Add to Cart
21717pBAD-mNectarinemNectarine fluorescent proteinsBacterial ExpressionRed fluorescent protein pH biosensor to detect concentrative nucleoside transport. J Biol Chem. 2009 Jun 3. ():. Bacterial expression of pH-sensitive monomeric mNectarine red fluorescent protein pBad/His B Add to Cart
25950cp193g7cp193g7Bacterial ExpressionCircularly permuted monomeric red fluorescent proteins with new termini in the beta-sheet. Protein Sci. 2010 Jun 2. ():. pBad/HisB Add to Cart
25951cp204cp204Bacterial ExpressionCircularly permuted monomeric red fluorescent proteins with new termini in the beta-sheet. Protein Sci. 2010 Jun 2. ():. pBad/HisB Add to Cart
25952cp196cp196Bacterial ExpressionCircularly permuted monomeric red fluorescent proteins with new termini in the beta-sheet. Protein Sci. 2010 Jun 2. ():. pBad/HisB Add to Cart
25953cp201cp201Bacterial ExpressionCircularly permuted monomeric red fluorescent proteins with new termini in the beta-sheet. Protein Sci. 2010 Jun 2. ():. pBad/HisB Add to Cart
25982cp193g7Ncp193g7NBacterial ExpressionCircularly permuted monomeric red fluorescent proteins with new termini in the beta-sheet. Protein Sci. 2010 Jun 2. ():. pBad/HisB Add to Cart
32442CMV-GEM-GECO1GEM-GECO1Mammalian ExpressionAn Expanded Palette of Genetically Encoded Ca2+ Indicators. Science. 2011 Sep 8. Mammalian expression of blue-green emission ratiometric genetically encoded Ca2+ indicator for optical imaging Customized Vector Add to Cart
32443CMV-GEX-GECO1GEX-GECO1.0Mammalian ExpressionAn Expanded Palette of Genetically Encoded Ca2+ Indicators. Science. 2011 Sep 8. Mammalian expression of green excitation ratiometric genetically encoded Ca2+ indicator for optical imaging Customized Vector Add to Cart
32444CMV-R-GECO1R-GECO1.0Mammalian ExpressionAn Expanded Palette of Genetically Encoded Ca2+ Indicators. Science. 2011 Sep 8. Mammalian expression of Red fluorescent genetically encoded Ca2+ indicator for optical imaging Customized Vector Add to Cart
32445CMV-G-GECO1.1G-GECO1.1Mammalian ExpressionAn Expanded Palette of Genetically Encoded Ca2+ Indicators. Science. 2011 Sep 8. Mammalian expression of green fluorescent genetically encoded Ca2+ indicator for optical imaging (version 1.1) Customized Vector Add to Cart
32446CMV-G-GECO1.2G-GECO1.2Mammalian ExpressionAn Expanded Palette of Genetically Encoded Ca2+ Indicators. Science. 2011 Sep 8. Mammalian expression of green fluorescent genetically encoded Ca2+ indicator for optical imaging (version 1.2) Customized Vector Add to Cart
32447CMV-G-GECO1.0G-GECO1.0Mammalian ExpressionAn Expanded Palette of Genetically Encoded Ca2+ Indicators. Science. 2011 Sep 8. Mammalian expression of green fluorescent genetically encoded Ca2+ indicator for optical imaging (version 1.0) Customized Vector Add to Cart
32448CMV-B-GECO1B-GECO1.0Mammalian ExpressionAn Expanded Palette of Genetically Encoded Ca2+ Indicators. Science. 2011 Sep 8. Mammalian expression of blue fluorescent genetically encoded Ca2+ indicator for optical imaging Customized Vector Add to Cart
32461CMV-mito-GEM-GECO1GEM-GECOMammalian ExpressionAn Expanded Palette of Genetically Encoded Ca2+ Indicators. Science. 2011 Sep 8. Mammalian expression of mitochondria-targeting blue-green emission ratiometric genetically encoded Ca2+ indicator for optical imaging pcDNA3.1 (-) Add to Cart
32462CMV-NLS-R-GECOR-GECO1.0Mammalian ExpressionAn Expanded Palette of Genetically Encoded Ca2+ Indicators. Science. 2011 Sep 8. Mammalian expression of nucleus-targeting red fluorescent genetically encoded Ca2+ indicator for optical imaging Customized Vector Add to Cart
32463pTorPE-GEM-GECO1GEM-GECO1Bacterial Expression ; Lab constructedAn Expanded Palette of Genetically Encoded Ca2+ Indicators. Science. 2011 Sep 8. Customized Vector Add to Cart
32464pTorPE-GEX-GECO1GEX-GECO1.0Bacterial Expression ; Lab constructedAn Expanded Palette of Genetically Encoded Ca2+ Indicators. Science. 2011 Sep 8. Customized Vector Add to Cart
32465pTorPE-R-GECO1R-GECO1.0Bacterial Expression ; Lab constructedAn Expanded Palette of Genetically Encoded Ca2+ Indicators. Science. 2011 Sep 8. Customized Vector Add to Cart
32466pTorPE-G-GECO1G-GECO1.0Bacterial Expression ; Lab constructedAn Expanded Palette of Genetically Encoded Ca2+ Indicators. Science. 2011 Sep 8. Customized Vector Add to Cart
32467pTorPE-G-GECO1.1G-GECO1.1Bacterial Expression ; Lab constructedAn Expanded Palette of Genetically Encoded Ca2+ Indicators. Science. 2011 Sep 8. Customized Vector Add to Cart
32468pTorPE-G-GECO1.2G-GECO1.2Bacterial Expression ; Lab constructedAn Expanded Palette of Genetically Encoded Ca2+ Indicators. Science. 2011 Sep 8. Customized Vector Add to Cart
32469pTorPE-B-GECO1B-GECO1.0Bacterial Expression ; Lab constructedAn Expanded Palette of Genetically Encoded Ca2+ Indicators. Science. 2011 Sep 8. Customized Vector Add to Cart
33067CaYin1CaYin1Bacterial ExpressionForster resonance energy transfer-based biosensors for multiparameter ratiometric imaging of Ca2+ dynamics and caspase-3 activity in single cells. Anal Chem. 2011 Dec 15;83(24):9687-93. doi: 10.1021/ac202595g. Epub 2011 Nov 28. pBAD hisB Add to Cart
33318CaYin1 NESCaYin1 NESMammalian ExpressionForster resonance energy transfer-based biosensors for multiparameter ratiometric imaging of Ca2+ dynamics and caspase-3 activity in single cells. Anal Chem. 2011 Dec 15;83(24):9687-93. doi: 10.1021/ac202595g. Epub 2011 Nov 28. pcDNA3 Add to Cart
36289pDDRFP-A1Dimerization dependent RFP A1Bacterial ExpressionA fluorogenic red fluorescent protein heterodimer. Chem Biol. 2012 Mar 23;19(3):353-60. pBad Add to Cart
36290pDDRFP-B1Dimerization dependent RFP-B1Bacterial ExpressionA fluorogenic red fluorescent protein heterodimer. Chem Biol. 2012 Mar 23;19(3):353-60. pBad Add to Cart
36291pDDRFP-A1B1Dimerization dependent RFP-A1B1Bacterial ExpressionA fluorogenic red fluorescent protein heterodimer. Chem Biol. 2012 Mar 23;19(3):353-60. pBad Add to Cart
36292pDDRFPA1-CaMddRFPA1-CaMMammalian ExpressionA fluorogenic red fluorescent protein heterodimer. Chem Biol. 2012 Mar 23;19(3):353-60. pcDNA3.1+ Add to Cart
36293pM13-DDRFPB1M13-DDRFP-B1Mammalian ExpressionA fluorogenic red fluorescent protein heterodimer. Chem Biol. 2012 Mar 23;19(3):353-60. pcDNA3.1+ Add to Cart
36294pDDRFP-A1B1-DEVDddRFPA1B1-DEVDMammalian ExpressionA fluorogenic red fluorescent protein heterodimer. Chem Biol. 2012 Mar 23;19(3):353-60. pcDNA3.1+ Add to Cart
40286pDDGFP-AddGFP-A (Synthetic)Bacterial ExpressionDimerization-dependent green and yellow fluorescent proteins. ACS Synth Biol. 2012 Dec 21;1(12):569-75. doi: 10.1021/sb300050j. Epub 2012 Aug 14. pBad-modified Add to Cart
40287pDDGFP-BddGFP-B (Synthetic)Bacterial ExpressionDimerization-dependent green and yellow fluorescent proteins. ACS Synth Biol. 2012 Dec 21;1(12):569-75. doi: 10.1021/sb300050j. Epub 2012 Aug 14. pBad-modified Add to Cart
40288pDDYFP-AddYFP-A (Synthetic)Bacterial ExpressionDimerization-dependent green and yellow fluorescent proteins. ACS Synth Biol. 2012 Dec 21;1(12):569-75. doi: 10.1021/sb300050j. Epub 2012 Aug 14. pBad-modified
  • Tag / Fusion Protein
    • His (N terminal on backbone)
  • Add to Cart
    40289pDDYFP-BddYFP-B (Synthetic)Bacterial ExpressionDimerization-dependent green and yellow fluorescent proteins. ACS Synth Biol. 2012 Dec 21;1(12):569-75. doi: 10.1021/sb300050j. Epub 2012 Aug 14. pBad-modified
  • Tag / Fusion Protein
    • His (N terminal on backbone)
  • Add to Cart
    40290pCalN-ddGFP-ACalnexin (Homo sapiens)Mammalian ExpressionDimerization-dependent green and yellow fluorescent proteins. ACS Synth Biol. 2012 Dec 21;1(12):569-75. doi: 10.1021/sb300050j. Epub 2012 Aug 14. pcDNA3.1 Add to Cart
    40291pTOM20-ddGFP-BTOMM20 (Homo sapiens)Mammalian ExpressionDimerization-dependent green and yellow fluorescent proteins. ACS Synth Biol. 2012 Dec 21;1(12):569-75. doi: 10.1021/sb300050j. Epub 2012 Aug 14. pcDNA3.1 TOMM20 MAS20, MOM19, TOM20 Add to Cart
    42170pcpmAmetrinecpmAmetrine173 (Synthetic)Bacterial ExpressionForster resonance energy transfer-based biosensors for multiparameter ratiometric imaging of Ca2+ dynamics and caspase-3 activity in single cells. Anal Chem. 2011 Dec 15;83(24):9687-93. doi: 10.1021/ac202595g. Epub 2011 Nov 28. pBAD-His B Add to Cart
    42171pmAmetrine1.2mAmetrine1.2 (Synthetic)Bacterial ExpressionForster resonance energy transfer-based biosensors for multiparameter ratiometric imaging of Ca2+ dynamics and caspase-3 activity in single cells. Anal Chem. 2011 Dec 15;83(24):9687-93. doi: 10.1021/ac202595g. Epub 2011 Nov 28. pBAD-His B Add to Cart
    42186pCMV-GR-GECO1.1GR-GECO1.1 (Synthetic)Highlightable Ca(2+) Indicators for Live Cell Imaging. J Am Chem Soc. 2012 Dec 26. modified pcDNA3.1 Add to Cart
    42187pCMV-GR-GECO1.2GR-GECO1.2 (Synthetic)Highlightable Ca(2+) Indicators for Live Cell Imaging. J Am Chem Soc. 2012 Dec 26. modified pcDNA3.1 Add to Cart
    42198pTorPE-GR-GECO1.1GR-GECO1.1 (Synthetic)Bacterial ExpressionHighlightable Ca(2+) Indicators for Live Cell Imaging. J Am Chem Soc. 2012 Dec 26. pTorPE Add to Cart
    42199pTorPE-GR-GECO1.2GR-GECO1.2 (Synthetic)Bacterial ExpressionHighlightable Ca(2+) Indicators for Live Cell Imaging. J Am Chem Soc. 2012 Dec 26. pTorPE Add to Cart
    45493CMV-CAR-GECO1CAR-GECO1 (Synthetic)Mammalian ExpressionImproved Orange and Red Ca Indicators and Photophysical Considerations for Optogenetic Applications. ACS Chem Neurosci. 2013 Mar 19. Carmine intensiometric genetically encoded Ca2+-indicators for optical imaging Customized Vector Add to Cart
    45494CMV-R-GECO1.2R-GECO1.2 (Synthetic)Mammalian ExpressionImproved Orange and Red Ca Indicators and Photophysical Considerations for Optogenetic Applications. ACS Chem Neurosci. 2013 Mar 19. Red intensiometric genetically encoded Ca2+-indicators for optical imaging 1.2 Customized Vector Add to Cart
    46021CMV-mito-R-GECO1R-GECO1 (Synthetic)Mammalian ExpressionImproved Orange and Red Ca Indicators and Photophysical Considerations for Optogenetic Applications. ACS Chem Neurosci. 2013 Mar 19. Red intensiometric genetically encoded Ca2+-indicators for optical imaging pcDNA3.1 (-) Add to Cart
    46022CMV-mito-CAR-GECO1CAR-GECO1 (Synthetic)Mammalian ExpressionImproved Orange and Red Ca Indicators and Photophysical Considerations for Optogenetic Applications. ACS Chem Neurosci. 2013 Mar 19. Carmine intensiometric genetically encoded Ca2+-indicators for optical imaging pcDNA3.1 (-) Add to Cart
    46025CMV-O-GECO1O-GECO1 (Synthetic)Mammalian ExpressionImproved Orange and Red Ca Indicators and Photophysical Considerations for Optogenetic Applications. ACS Chem Neurosci. 2013 Mar 19. Orange intensiometric genetically encoded Ca2+-indicators for optical imaging Customized Vector Add to Cart
    50835GANLS-DEVD-BNESddGFP A and ddRFP B (Synthetic)Mammalian ExpressionRatiometric biosensors based on dimerization-dependent fluorescent protein exchange. Nat Methods. 2015 Jan 26. doi: 10.1038/nmeth.3261. Expresses a tandem ddFP heterodimer in mammalian cells, construct 1 for a translocation-based green fluorescent caspase-3 biosensor, used together with plasmid 2 (BNLS). pcDNA3.1 Add to Cart
    50836BNLSddGFP B (Synthetic)Mammalian ExpressionRatiometric biosensors based on dimerization-dependent fluorescent protein exchange. Nat Methods. 2015 Jan 26. doi: 10.1038/nmeth.3261. Expresses a tandem ddFP heterodimer in mammalian cells, construct 1 for a translocation-based green fluorescent caspase-3 biosensor, used together with plasmid 1 (GANLS-DEVD-BNES or RANLS-DEVD-BNES). pcDNA3.1 Add to Cart
    50840RANLS-DEVD-BNESddRFP A and ddRFP B (Synthetic)Mammalian ExpressionRatiometric biosensors based on dimerization-dependent fluorescent protein exchange. Nat Methods. 2015 Jan 26. doi: 10.1038/nmeth.3261. Expresses a tandem ddFP heterodimer in mammalian cells, plasmid 1 for a translocation-based red fluorescent caspase-3 biosensor, used together with plasmid 2 (BNLS). pcDNA3.1 Add to Cart
    50842GANES-DEVD-BNLSddGFP A and ddGFP B (Synthetic)Mammalian ExpressionRatiometric biosensors based on dimerization-dependent fluorescent protein exchange. Nat Methods. 2015 Jan 26. doi: 10.1038/nmeth.3261. Expresses a tandem green ddFP heterodimer in mammalian cells, construct 1 for a green to red colour switch-based fluorescent caspase-3 biosensor, used together with a second plasmid RANLS. pcDNA3.1 Add to Cart
    50843RANLSddRFP A (Synthetic)Mammalian ExpressionRatiometric biosensors based on dimerization-dependent fluorescent protein exchange. Nat Methods. 2015 Jan 26. doi: 10.1038/nmeth.3261. Expresses one ddFP in mammalian cells, the second plasmid for a green to red colour switch-based fluorescent caspase-3 biosensor, used together with plasmid GANES-DEVD-BNLS. pcDNA3.1 Add to Cart
    50849NESRA-DEVD-BNLSddRFP A and ddRFP B (Synthetic)Mammalian ExpressionRatiometric biosensors based on dimerization-dependent fluorescent protein exchange. Nat Methods. 2015 Jan 26. doi: 10.1038/nmeth.3261. Expresses a tandem red ddFP heterodimer in mammalian cells, construct 1 for a red to green colour switch-based fluorescent caspase-3 biosensor, used together with a second plasmid GANLS. pcDNA3.1 Add to Cart
    50852GANLSddGFP A (Synthetic)Mammalian ExpressionRatiometric biosensors based on dimerization-dependent fluorescent protein exchange. Nat Methods. 2015 Jan 26. doi: 10.1038/nmeth.3261. Expresses one ddFP in mammalian cells, the second plasmid for a red to green colour switch-based fluorescent caspase-3 biosensor, used together with plasmid NESRA-DEVD-BNLS. pcDNA3.1 Add to Cart
    52099CH-GECO2.1CH-GECO2.1 (Synthetic)Bacterial ExpressionCircular permutated red fluorescent proteins and calcium ion indicators based on mCherry. Protein Eng Des Sel. 2013 Dec;26(12):763-72. doi: 10.1093/protein/gzt052. Epub 2013 Oct 22. mCherry-based Calcium indicators pBAD/HisB Add to Cart
    52100cp196V1.2cp196V1.2 (Synthetic)Bacterial ExpressionCircular permutated red fluorescent proteins and calcium ion indicators based on mCherry. Protein Eng Des Sel. 2013 Dec;26(12):763-72. doi: 10.1093/protein/gzt052. Epub 2013 Oct 22. A improved cpmCherry variant pBAD/HisB Add to Cart
    52304pcDNA3.1 mPapaya1.0fluorescent protein mpapaya (Synthetic)Mammalian ExpressionAn engineered monomeric Zoanthus sp. yellow fluorescent protein. Chem Biol. 2013 Oct 24;20(10):1296-304. doi: 10.1016/j.chembiol.2013.08.008. Epub 2013 Oct 3. An engineered monomeric Zoanthus sp. yellow fluorescent protein called mpapaya pcDNA3.1 Add to Cart
    54505mAmetrine-N1Mammalian ExpressionFluorescent protein FRET pairs for ratiometric imaging of dual biosensors. Nat Methods. 2008 May . 5(5):401-3. Localization: N1 Cloning Vector, Excitation: 406, Emission: 526 mAmetrine-N1
  • Tag / Fusion Protein
    • mAmetrine (C terminal on backbone)
  • Add to Cart
    54524mPapaya-C1Mammalian ExpressionAn engineered monomeric Zoanthus sp. yellow fluorescent protein. Chem Biol. 2013 Oct 24;20(10):1296-304. doi: 10.1016/j.chembiol.2013.08.008. Epub 2013 Oct 3. Localization: C1 Cloning Vector, Excitation: 530, Emission: 541 mPapaya-C1
  • Tag / Fusion Protein
    • mPapaya (N terminal on backbone)
  • Add to Cart
    54540mPapaya-Lifeact-7Mammalian ExpressionAn engineered monomeric Zoanthus sp. yellow fluorescent protein. Chem Biol. 2013 Oct 24;20(10):1296-304. doi: 10.1016/j.chembiol.2013.08.008. Epub 2013 Oct 3. Localization: Actin, Excitation: 530, Emission: 541 mPapaya-Lifeact
  • Tag / Fusion Protein
    • mPapaya-Lifeact (C terminal on backbone)
  • Add to Cart
    54545mT-Sapphire-C1Mammalian ExpressionFluorescent protein FRET pairs for ratiometric imaging of dual biosensors. Nat Methods. 2008 May . 5(5):401-3. Localization: C1 Cloning Vector, Excitation: 399, Emission: 511 mT-Sapphire-C1
  • Tag / Fusion Protein
    • mT-Sapphire (N terminal on backbone)
  • Add to Cart
    54569mT-Sapphire-N1Mammalian ExpressionFluorescent protein FRET pairs for ratiometric imaging of dual biosensors. Nat Methods. 2008 May . 5(5):401-3. Localization: N1 Cloning Vector, Excitation: 399, Emission: 511 mT-Sapphire-N1
  • Tag / Fusion Protein
    • mT (C terminal on backbone)
  • Add to Cart
    54571mT-Sapphire-pBADBacterial ExpressionFluorescent protein FRET pairs for ratiometric imaging of dual biosensors. Nat Methods. 2008 May . 5(5):401-3. Localization: Protein Expression Vector , Excitation: 399, Emission: 511 mT-Sapphire-pBAD
  • Tags / Fusion Proteins
    • mT (N terminal on backbone)
    • 6xHis (N terminal on backbone)
  • Add to Cart
    54587mCitrine-C1Mammalian Expression Michael Davidson Empty Backbones (unpublished) Localization: C1 Cloning Vector, Excitation: 516, Emission: 529 mCitrine-C1
  • Tag / Fusion Protein
    • mCitrine (N terminal on backbone)
  • Add to Cart
    54593Citrine-N1Mammalian Expression Michael Davidson Empty Backbones (unpublished) Localization: N1 Cloning Vector, Excitation: 516, Emission: 529 Citrine-N1
  • Tag / Fusion Protein
    • Citrine (C terminal on backbone)
  • Add to Cart
    54594mCitrine-N1Mammalian Expression Michael Davidson Empty Backbones (unpublished) Localization: N1 Cloning Vector, Excitation: 516, Emission: 529 mCitrine-N1
  • Tag / Fusion Protein
    • mCitrine (C terminal on backbone)
  • Add to Cart
    54635mRFP1-N1Mammalian Expression Michael Davidson Empty Backbones (unpublished) Localization: N1 Cloning Vector, Excitation: 584, Emission: 607 mRFP1-N1
  • Tag / Fusion Protein
    • mRFP1 (C terminal on backbone)
  • Add to Cart
    54660mAmetrine-C1Mammalian ExpressionFluorescent protein FRET pairs for ratiometric imaging of dual biosensors. Nat Methods. 2008 May . 5(5):401-3. Localization: C1 Cloning Vector, Excitation: 406, Emission: 526 mAmetrine-C1
  • Tag / Fusion Protein
    • mAmetrine (N terminal on backbone)
  • Add to Cart
    54667mRFP1-pBADBacterial Expression Michael Davidson Empty Backbones (unpublished) Localization: Protein Expression Vector , Excitation: 584, Emission: 607 mRFP1-pBAD
  • Tags / Fusion Proteins
    • mRFP1 (N terminal on backbone)
    • 6xHis (N terminal on backbone)
  • Add to Cart
    54693mPapaya1-Lifeact-7Mammalian ExpressionAn engineered monomeric Zoanthus sp. yellow fluorescent protein. Chem Biol. 2013 Oct 24;20(10):1296-304. doi: 10.1016/j.chembiol.2013.08.008. Epub 2013 Oct 3. Localization: Actin, Excitation: 530, Emission: 541 mPapaya1-Lifeact
  • Tag / Fusion Protein
    • mPapaya1-Lifeact (C terminal on backbone)
  • Add to Cart
    54714mNeptune-pBADBacterial Expression Michael Davidson Empty Backbones (unpublished) Localization: Protein Expression Vector , Excitation: 600, Emission: 650 mNeptune-pBAD
  • Tags / Fusion Proteins
    • mNeptune (N terminal on backbone)
    • 6xHis (N terminal on backbone)
  • Add to Cart
    54715Citrine-C1Mammalian Expression Michael Davidson Empty Backbones (unpublished) Localization: C1 Cloning Vector, Excitation: 516, Emission: 529 Citrine-C1
  • Tag / Fusion Protein
    • Citrine (N terminal on backbone)
  • Add to Cart
    54716mPapaya-N1Mammalian ExpressionAn engineered monomeric Zoanthus sp. yellow fluorescent protein. Chem Biol. 2013 Oct 24;20(10):1296-304. doi: 10.1016/j.chembiol.2013.08.008. Epub 2013 Oct 3. Localization: N1 Cloning Vector, Excitation: 530, Emission: 541 mPapaya-N1
  • Tag / Fusion Protein
    • mPapaya (C terminal on backbone)
  • Add to Cart
    54722mPapaya1-C1Mammalian ExpressionAn engineered monomeric Zoanthus sp. yellow fluorescent protein. Chem Biol. 2013 Oct 24;20(10):1296-304. doi: 10.1016/j.chembiol.2013.08.008. Epub 2013 Oct 3. Localization: C1 Cloning Vector, Excitation: 530, Emission: 541 mPapaya1-C1
  • Tag / Fusion Protein
    • mPapaya1 (N terminal on backbone)
  • Add to Cart
    54723mCitrine-pBADBacterial Expression Michael Davidson Empty Backbones (unpublished) Localization: Protein Expression Vector , Excitation: 516, Emission: 529 mCitrine-pBAD
  • Tags / Fusion Proteins
    • mCitrine (N terminal on backbone)
    • 6xHis (N terminal on backbone)
  • Add to Cart
    54732mPapaya1-N1Mammalian ExpressionAn engineered monomeric Zoanthus sp. yellow fluorescent protein. Chem Biol. 2013 Oct 24;20(10):1296-304. doi: 10.1016/j.chembiol.2013.08.008. Epub 2013 Oct 3. Localization: N1 Cloning Vector, Excitation: 530, Emission: 541 mPapaya1-N1
  • Tag / Fusion Protein
    • mPapaya1 (C terminal on backbone)
  • Add to Cart
    54764mRFP1-C1Mammalian Expression Michael Davidson Empty Backbones (unpublished) Localization: C1 Cloning Vector, Excitation: 584, Emission: 607 mRFP1-C1
  • Tag / Fusion Protein
    • mRFP1 (N terminal on backbone)
  • Add to Cart
    54772Citrine-pBADBacterial Expression Michael Davidson Empty Backbones (unpublished) Localization: Protein Expression Vector , Excitation: 516, Emission: 529 Citrine-pBAD
  • Tags / Fusion Proteins
    • Citrine (N terminal on backbone)
    • 6xHis (N terminal on backbone)
  • Add to Cart
    54838mPapaya-pBADBacterial ExpressionAn engineered monomeric Zoanthus sp. yellow fluorescent protein. Chem Biol. 2013 Oct 24;20(10):1296-304. doi: 10.1016/j.chembiol.2013.08.008. Epub 2013 Oct 3. Localization: Protein Expression Vector , Excitation: 530, Emission: 541 mPapaya-pBAD
  • Tags / Fusion Proteins
    • mPapaya (N terminal on backbone)
    • 6xHis (N terminal on backbone)
  • Add to Cart
    55765pTorPE-Y-GECO1Y-GECO1.0 (Synthetic)Bacterial Expression ; Lab constructedMicrofluidic cell sorter-aided directed evolution of a protein-based calcium ion indicator with an inverted fluorescent response. Integr Biol (Camb). 2014 May 19. Encoded a yellow fluorescent calcium ion indicator with inverted fluorescence response for expression in bacterial cells Customized Vector Add to Cart
    55766CMV-Y-GECO1Y-GECO1.0 (Synthetic)Mammalian ExpressionMicrofluidic cell sorter-aided directed evolution of a protein-based calcium ion indicator with an inverted fluorescent response. Integr Biol (Camb). 2014 May 19. Encoded a yellow fluorescent calcium ion indicator with inverted fluorescence response for expression in mammalian cells Customized Vector Add to Cart
    55767AAV-Y-GECO1Y-GECO1.0 (Synthetic)Mammalian Expression, AAVMicrofluidic cell sorter-aided directed evolution of a protein-based calcium ion indicator with an inverted fluorescent response. Integr Biol (Camb). 2014 May 19. Express Y-GECO1 in neurons pAAV Add to Cart
    58200AAV-hSyn-Lyn-Y-GECO1Y-GECO1.0 (Synthetic)AAVMicrofluidic cell sorter-aided directed evolution of a protein-based calcium ion indicator with an inverted fluorescent response. Integr Biol (Camb). 2014 May 19. Express the membrane targeted Y-GECO1 in neurons AAV with hSyn promoter Add to Cart
    60883single polypeptide FPX biosensor for caspase-3single polypeptide FPX biosensor for caspase-3 (Synthetic)Mammalian ExpressionRatiometric biosensors based on dimerization-dependent fluorescent protein exchange. Nat Methods. 2015 Jan 26. doi: 10.1038/nmeth.3261. FPX biosensor, a new class of genetically encoded fluorescent biosensor based on reversible exchange of the heterodimeric partners of green and red dimerization dependent fluorescent proteins (ddFPs). pcDNA3 (+) Add to Cart
    60884single polypeptide FPX biosensor for caspase-8single polypeptide FPX biosensor for caspase-8 (Synthetic)Mammalian ExpressionRatiometric biosensors based on dimerization-dependent fluorescent protein exchange. Nat Methods. 2015 Jan 26. doi: 10.1038/nmeth.3261. FPX biosensor for caspase-8 pcDNA3 (+) Add to Cart
    60885single polypeptide FPX biosensor for caspase-1single polypeptide FPX biosensor for caspase-1 (Synthetic)Mammalian ExpressionRatiometric biosensors based on dimerization-dependent fluorescent protein exchange. Nat Methods. 2015 Jan 26. doi: 10.1038/nmeth.3261. FPX biosensor for caspase-1 pcDNA3 (+) Add to Cart
    60886single polypeptide FPX biosensor for both caspase-3 and -8single polypeptide FPX biosensor for caspase-3 and 8 (Synthetic)Mammalian ExpressionRatiometric biosensors based on dimerization-dependent fluorescent protein exchange. Nat Methods. 2015 Jan 26. doi: 10.1038/nmeth.3261. FPX biosensor pcDNA3 (+) Add to Cart
    60887single polypeptide FPX biosensor for calcium ionsingle polypeptide FPX biosensor for calcium ion (Synthetic)Mammalian ExpressionRatiometric biosensors based on dimerization-dependent fluorescent protein exchange. Nat Methods. 2015 Jan 26. doi: 10.1038/nmeth.3261. FPX biosensor pcDNA3 (+) Add to Cart
    60972RANLS-IETD-BNESddRFP A and ddRFP B (Synthetic)Mammalian ExpressionRatiometric biosensors based on dimerization-dependent fluorescent protein exchange. Nat Methods. 2015 Jan 26. doi: 10.1038/nmeth.3261. Expresses a tandem ddFP heterodimer in mammalian cells, plasmid 1 for a translocation-based red fluorescent caspase-8 biosensor, used together with plasmid 2 (BNLS). pcDNA3.1 Add to Cart
    60973RANLS-LEHD-BNESddRFP A and ddRFP B (Synthetic)Mammalian ExpressionRatiometric biosensors based on dimerization-dependent fluorescent protein exchange. Nat Methods. 2015 Jan 26. doi: 10.1038/nmeth.3261. Expresses a tandem ddFP heterodimer in mammalian cells, plasmid 1 for a translocation-based red fluorescent caspase-9 biosensor, used together with plasmid 2 (BNLS). pcDNA3.1 Add to Cart
    60974Ratiometric FPX sensor for ERK kinase activityddRFP A-WW domain-ERK substrate-ddFP B (Synthetic)Mammalian ExpressionRatiometric biosensors based on dimerization-dependent fluorescent protein exchange. Nat Methods. 2015 Jan 26. doi: 10.1038/nmeth.3261. For imaging of ERK kinase activity using a single polypeptide RA-WW domain-ERK substrate-B protein and free GA-NES. pcDNA3.0 Add to Cart
    61017GB-NESddGFP B (Synthetic)Mammalian ExpressionRatiometric biosensors based on dimerization-dependent fluorescent protein exchange. Nat Methods. 2015 Jan 26. doi: 10.1038/nmeth.3261. ddGFP B-NES pcDNA3.1 Add to Cart
    61018GA-NESddGFP A (Synthetic)Mammalian ExpressionRatiometric biosensors based on dimerization-dependent fluorescent protein exchange. Nat Methods. 2015 Jan 26. doi: 10.1038/nmeth.3261. ddGFP A-NES pcDNA3.1 Add to Cart
    61019RA-NESddRFP A (Synthetic)Mammalian ExpressionRatiometric biosensors based on dimerization-dependent fluorescent protein exchange. Nat Methods. 2015 Jan 26. doi: 10.1038/nmeth.3261. ddRFP A-NES pcDNA3.1 Add to Cart
    61020GA-DEVD-GB-NESddGFP A and ddGFP B (Synthetic)Mammalian ExpressionRatiometric biosensors based on dimerization-dependent fluorescent protein exchange. Nat Methods. 2015 Jan 26. doi: 10.1038/nmeth.3261. green ddFP based caspase-3 biosensor pcDNA3 (+) Add to Cart
    61244CMV-ER-LAR-GECO1LAR-GECO1 (Synthetic)Mammalian ExpressionRed fluorescent genetically encoded Ca2+ indicators for use in mitochondria and endoplasmic reticulum. Biochem J. 2014 Nov 15;464(1):13-22. doi: 10.1042/BJ20140931. Expresses LAR-GECO1 in the endoplasmic reticulum in mammalian cells Customized Vector Add to Cart
    61245CMV-mito-LAR-GECO1.2LAR-GECO1.2 (Synthetic)Mammalian ExpressionRed fluorescent genetically encoded Ca2+ indicators for use in mitochondria and endoplasmic reticulum. Biochem J. 2014 Nov 15;464(1):13-22. doi: 10.1042/BJ20140931. Expresses LAR-GECO1.2 in the mitochondria in mammalian cells pcDNA3.1 (-) Add to Cart
    61246CMV-REX-GECO1REX-GECO1 (Synthetic)Mammalian ExpressionA long Stokes shift red fluorescent Ca(2+) indicator protein for two-photon and ratiometric imaging. Nat Commun. 2014 Oct 31;5:5262. doi: 10.1038/ncomms6262. Expresses REX-GECO1 in mammalian cells Customized Vector Add to Cart
    61247CMV-REX-GECO0.9REX-GECO0.9 (Synthetic)Mammalian ExpressionA long Stokes shift red fluorescent Ca(2+) indicator protein for two-photon and ratiometric imaging. Nat Commun. 2014 Oct 31;5:5262. doi: 10.1038/ncomms6262. Expresses REX-GECO0.9 in mammalian cells Customized Vector Add to Cart
    61248AAV-hSyn-REX-GECO1REX-GECO1 (Synthetic)Mammalian Expression, AAVA long Stokes shift red fluorescent Ca(2+) indicator protein for two-photon and ratiometric imaging. Nat Commun. 2014 Oct 31;5:5262. doi: 10.1038/ncomms6262. Expresses REX-GECO1 in neurons pAAV Add to Cart
    61249AAV-hSyn-REX-GECO0.9REX-GECO0.9 (Synthetic)Mammalian Expression, AAVA long Stokes shift red fluorescent Ca(2+) indicator protein for two-photon and ratiometric imaging. Nat Commun. 2014 Oct 31;5:5262. doi: 10.1038/ncomms6262. Expresses REX-GECO0.9 in neurons pAAV Add to Cart
    61555pBAD-pHujipHuji (Synthetic)Bacterial ExpressionpHuji, a pH-sensitive red fluorescent protein for imaging of exo- and endocytosis. J Cell Biol. 2014 Nov 10;207(3):419-32. doi: 10.1083/jcb.201404107. Red fluorescent protein based pH indicator in bacterial expression vector pBAD/His-B Add to Cart
    61556pDisplay-pHujipHuji (Synthetic)Mammalian ExpressionpHuji, a pH-sensitive red fluorescent protein for imaging of exo- and endocytosis. J Cell Biol. 2014 Nov 10;207(3):419-32. doi: 10.1083/jcb.201404107. Red fluorescent protein based pH indicator in pDisplay vector pDisplay Add to Cart
    61557pDisplay-pHoran4pHoran4 (Synthetic)Mammalian ExpressionpHuji, a pH-sensitive red fluorescent protein for imaging of exo- and endocytosis. J Cell Biol. 2014 Nov 10;207(3):419-32. doi: 10.1083/jcb.201404107. Orange fluorescent protein based pH indicator in pDisplay vector pDisplay Add to Cart
    61558pBAD-pHoran4pHoran4 (Synthetic)Bacterial ExpressionpHuji, a pH-sensitive red fluorescent protein for imaging of exo- and endocytosis. J Cell Biol. 2014 Nov 10;207(3):419-32. doi: 10.1083/jcb.201404107. Orange fluorescent protein based pH indicator in bacterial expression vector pBAD/His-B Add to Cart
    74142CMV-FlicR1FlicR1 (Synthetic)Mammalian Expression, Bacterial ExpressionA Bright and Fast Red Fluorescent Protein Voltage Indicator That Reports Neuronal Activity in Organotypic Brain Slices. J Neurosci. 2016 Feb 24;36(8):2458-72. doi: 10.1523/JNEUROSCI.3484-15.2016. Mammalian expression of FlicR1: A bright red fluorescent genetically encoded voltage indicator pcDNA3.1 Add to Cart
    74143AAV-hSyn-FlicR1FlicR1 (Synthetic)Mammalian Expression, AAVA Bright and Fast Red Fluorescent Protein Voltage Indicator That Reports Neuronal Activity in Organotypic Brain Slices. J Neurosci. 2016 Feb 24;36(8):2458-72. doi: 10.1523/JNEUROSCI.3484-15.2016. Express FlicR1 preferentially in neurons pAAV Add to Cart