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Scot Wolfe Lab Plasmids


The Scot Wolfe Lab has deposited plasmids at Addgene for distribution to the research community. Addgene is a nonprofit plasmid repository dedicated to improving life science research.

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Title Authors Publication
A bacterial one-hybrid system for determining the DNA-binding specificity of transcription factors. Meng X, Brodsky MH, Wolfe SA Nat Biotechnol. 2005 Aug . 23(8):988-94.
A systematic characterization of factors that regulate Drosophila segmentation via a bacterial one-hybrid system. Noyes MB, Meng X, Wakabayashi A, Sinha S, Brodsky MH, Wolfe SA Nucleic Acids Res. 2008 Mar 10. ():.
Targeted gene inactivation in zebrafish using engineered zinc-finger nucleases. Meng X, Noyes MB, Zhu LJ, Lawson ND, Wolfe SA Nat Biotechnol. 2008 Jun . 26(6):695-701.
Unpublished Plasmids (unpublished)
Evaluation and application of modularly assembled zinc-finger nucleases in zebrafish. Zhu C, Smith T, McNulty J, Rayla AL, Lakshmanan A, Siekmann AF, Buffardi M, Meng X, Shin J, Padmanabhan A, Cifuentes D, Giraldez AJ, Look AT, Epstein JA, Lawson ND, Wolfe SA Development. 2011 Oct;138(20):4555-64.
Cas9 effector-mediated regulation of transcription and differentiation in human pluripotent stem cells. Kearns NA, Genga RM, Enuameh MS, Garber M, Wolfe SA, Maehr R Development. 2014 Jan;141(1):219-23. doi: 10.1242/dev.103341.
Improved U6-sgRNA vector for expression (unpublished)
DNA-binding-domain fusions enhance the targeting range and precision of Cas9. Bolukbasi MF, Gupta A, Oikemus S, Derr AG, Garber M, Brodsky MH, Zhu LJ, Wolfe SA Nat Methods. 2015 Oct 19. doi: 10.1038/nmeth.3624.
pLKO.1-puro U6 N. meningitidis sgRNA BfuAI large stuffer (unpublished)
ID Kit Hidden Extra Search Info  
1000000018Wolfe-Lawson ZFN modular assembly kit Add to Cart
ID Plasmid Gene/Insert Vector Type Publication Hidden Extra Search Info  
12609pH3U3-mcsMultiple cloning site in His3 Ura3 reporter plasmid (Saccharomyces cerevisiae)Bacterial ExpressionA bacterial one-hybrid system for determining the DNA-binding specificity of transcription factors. Nat Biotechnol. 2005 Aug . 23(8):988-94. pH3U3 Add to Cart
12610pH3U3-Zif268Zif268 binding site in pH3U3Bacterial ExpressionA bacterial one-hybrid system for determining the DNA-binding specificity of transcription factors. Nat Biotechnol. 2005 Aug . 23(8):988-94. PH3U3-mcs Add to Cart
12611pB1H1Dorsal RHR in pB1H1 (Drosophila melanogaster)Bacterial ExpressionA bacterial one-hybrid system for determining the DNA-binding specificity of transcription factors. Nat Biotechnol. 2005 Aug . 23(8):988-94. pB1H1 dl Dmel_CG6667, CG6667, DL, Dl, Dmel\CG6667, Dor, Dorsal, GSd447, NFkappaB, REL, anon-EST:GressD7, d, dL, fs(2)k10816, mat(2)dorsal Add to Cart
12612pB1H1-Zif268Zif268 in pB1H1 (Mus musculus)Bacterial ExpressionA bacterial one-hybrid system for determining the DNA-binding specificity of transcription factors. Nat Biotechnol. 2005 Aug . 23(8):988-94. pB1H1 Egr1 A530045N19Rik, ETR103, Egr-1, Krox-1, Krox-24, Krox24, NGF1-A, NGFI-A, NGFIA, TIS8, Zenk, Zfp-6, Zif268, egr Add to Cart
12613pB1H2dorsal RHR in pB1H2 (Drosophila melanogaster)Bacterial ExpressionA bacterial one-hybrid system for determining the DNA-binding specificity of transcription factors. Nat Biotechnol. 2005 Aug . 23(8):988-94. pB1H2 dl Dmel_CG6667, CG6667, DL, Dl, Dmel\CG6667, Dor, Dorsal, GSd447, NFkappaB, REL, anon-EST:GressD7, d, dL, fs(2)k10816, mat(2)dorsal Add to Cart
12614US0hisB-pyrF-Bacteria hybrid selection strainBacterial ExpressionA bacterial one-hybrid system for determining the DNA-binding specificity of transcription factors. Nat Biotechnol. 2005 Aug . 23(8):988-94. XL-1 blue F' episome Add to Cart
18038pB1H2w2-PrdPaired (Drosophila melanogaster)Bacterial ExpressionA systematic characterization of factors that regulate Drosophila segmentation via a bacterial one-hybrid system. Nucleic Acids Res. 2008 Mar 10. ():. ColE1 prd CG6716, Dmel\CG6716, pr, Prd, PRD Add to Cart
18039pB1H2w5-PrdPaired (Drosophila melanogaster)Bacterial ExpressionA systematic characterization of factors that regulate Drosophila segmentation via a bacterial one-hybrid system. Nucleic Acids Res. 2008 Mar 10. ():. ColE1 prd CG6716, Dmel\CG6716, pr, Prd, PRD Add to Cart
18040pB1H2wL-PrdPaired (Drosophila melanogaster)Bacterial ExpressionA systematic characterization of factors that regulate Drosophila segmentation via a bacterial one-hybrid system. Nucleic Acids Res. 2008 Mar 10. ():. ColE1 prd CG6716, Dmel\CG6716, pr, Prd, PRD Add to Cart
18041pB1H2w2-12-Oct1HD of Oct1Bacterial ExpressionA systematic characterization of factors that regulate Drosophila segmentation via a bacterial one-hybrid system. Nucleic Acids Res. 2008 Mar 10. ():. ColE1 Add to Cart
18042pB1H2w2-12-C81SHhaI C81S mutantBacterial ExpressionA systematic characterization of factors that regulate Drosophila segmentation via a bacterial one-hybrid system. Nucleic Acids Res. 2008 Mar 10. ():. ColE1 Add to Cart
18043pB1H2w5-12-C81SHhaI C81S mutantBacterial ExpressionA systematic characterization of factors that regulate Drosophila segmentation via a bacterial one-hybrid system. Nucleic Acids Res. 2008 Mar 10. ():. ColE1 Add to Cart
18044pB1H2w2-mutOddmutant Odd Skipped (Drosophila melanogaster)Bacterial ExpressionA systematic characterization of factors that regulate Drosophila segmentation via a bacterial one-hybrid system. Nucleic Acids Res. 2008 Mar 10. ():. ColE1 Add to Cart
18045pB1H2w2-zif268zif268 (Mus musculus)Bacterial ExpressionA systematic characterization of factors that regulate Drosophila segmentation via a bacterial one-hybrid system. Nucleic Acids Res. 2008 Mar 10. ():. ColE1 Add to Cart
18046pH3U3-zif268 (omega)zif268 binding siteBacterial ExpressionA systematic characterization of factors that regulate Drosophila segmentation via a bacterial one-hybrid system. Nucleic Acids Res. 2008 Mar 10. ():. pH3U3 Add to Cart
18049USO hisB- pyrF- rpoZ-omega knockout Bacteria hybrid selection strainBacterial ExpressionA systematic characterization of factors that regulate Drosophila segmentation via a bacterial one-hybrid system. Nucleic Acids Res. 2008 Mar 10. ():. XL-1 blue F' episome Add to Cart
187521352wUV2 F2bbs kan GCANNNAGGk3 tandem zinc fingers (Mus musculus)Bacterial ExpressionTargeted gene inactivation in zebrafish using engineered zinc-finger nucleases. Nat Biotechnol. 2008 Jun . 26(6):695-701. ColE1 Add to Cart
187531352wUV2 F1bbs kan GCGTGGNNN3 tandem zinc fingers (Mus musculus)Bacterial ExpressionTargeted gene inactivation in zebrafish using engineered zinc-finger nucleases. Nat Biotechnol. 2008 Jun . 26(6):695-701. ColE1 Add to Cart
18754pCS2-HA-GAAZFP-FokI-RRzinc finger nuclease (Mus musculus)Bacterial ExpressionTargeted gene inactivation in zebrafish using engineered zinc-finger nucleases. Nat Biotechnol. 2008 Jun . 26(6):695-701. CS2 Add to Cart
18755pCS2-Flag-TTGZFP-FokI-DDzinc finger nuclease (Mus musculus)Bacterial ExpressionTargeted gene inactivation in zebrafish using engineered zinc-finger nucleases. Nat Biotechnol. 2008 Jun . 26(6):695-701. CS2 Add to Cart
187561352wUV2 F3bbs kan NNNTGGGCGk3 tandem zinc fingers (Mus musculus)Bacterial ExpressionTargeted gene inactivation in zebrafish using engineered zinc-finger nucleases. Nat Biotechnol. 2008 Jun . 26(6):695-701. ColE1 Add to Cart
207041352wUV2-kpn1-bamH1-stop4 finger ZFPBacterial Expression Unpublished Plasmids (unpublished) ColE1 Add to Cart
30687pBS-F1-AAGF1 AAGCloning vectorEvaluation and application of modularly assembled zinc-finger nucleases in zebrafish. Development. 2011 Oct;138(20):4555-64. pBlueScript II SK(+) Add to Cart
30688pBS-F1-ACGF1 ACGCloning vectorEvaluation and application of modularly assembled zinc-finger nucleases in zebrafish. Development. 2011 Oct;138(20):4555-64. pBlueScript II SK(+) Add to Cart
30689pBS-F1-AGGF1 AGGCloning vectorEvaluation and application of modularly assembled zinc-finger nucleases in zebrafish. Development. 2011 Oct;138(20):4555-64. pBlueScript II SK(+) Add to Cart
30690pBS-F1-ATGF1 ATGCloning vectorEvaluation and application of modularly assembled zinc-finger nucleases in zebrafish. Development. 2011 Oct;138(20):4555-64. pBlueScript II SK(+) Add to Cart
30691pCS2-F1-CAG-F2-GACF1 CAG, F2 GACmRNA productionEvaluation and application of modularly assembled zinc-finger nucleases in zebrafish. Development. 2011 Oct;138(20):4555-64. pCS2 (modified) Add to Cart
30692pBS-F1-CGGF1 CGGCloning vectorEvaluation and application of modularly assembled zinc-finger nucleases in zebrafish. Development. 2011 Oct;138(20):4555-64. pBlueScript II SK(+) Add to Cart
30693pBS-F1-GAAF1 GAACloning vectorEvaluation and application of modularly assembled zinc-finger nucleases in zebrafish. Development. 2011 Oct;138(20):4555-64. pBlueScript II SK(+) Add to Cart
30694pCS2_F1-GACF1 GAC (Danio rerio)mRNA productionEvaluation and application of modularly assembled zinc-finger nucleases in zebrafish. Development. 2011 Oct;138(20):4555-64. pCS2 (modified) Add to Cart
30695pCS2-F1-GAG-F2-GAG-F3-AAGF1 GAG, F2 GAG, F3 AAGmRNA productionEvaluation and application of modularly assembled zinc-finger nucleases in zebrafish. Development. 2011 Oct;138(20):4555-64. pCS2 (modified) Add to Cart
30696pCS2-F2-GAAF2 GAA (Danio rerio)mRNA productionEvaluation and application of modularly assembled zinc-finger nucleases in zebrafish. Development. 2011 Oct;138(20):4555-64. pCS2 Add to Cart
30697pCS2-F1-GCAF1 GCA (Danio rerio)mRNA productionEvaluation and application of modularly assembled zinc-finger nucleases in zebrafish. Development. 2011 Oct;138(20):4555-64. pCS2 Add to Cart
30698pCS2-F1-GCCF1 GCC (Danio rerio)mRNA productionEvaluation and application of modularly assembled zinc-finger nucleases in zebrafish. Development. 2011 Oct;138(20):4555-64. pCS2 Add to Cart
30699pCS2-F1-GCGF1 GCG (Danio rerio)mRNA productionEvaluation and application of modularly assembled zinc-finger nucleases in zebrafish. Development. 2011 Oct;138(20):4555-64. pCS2 Add to Cart
30700pCS2-F1-GCTF1 GCT (Danio rerio)mRNA productionEvaluation and application of modularly assembled zinc-finger nucleases in zebrafish. Development. 2011 Oct;138(20):4555-64. pCS2 (modified) Add to Cart
30701pCS2-F1-GGA-F2-GTG-F3-TTGF1 GGA, F2 GTG, F3 TTGmRNA productionEvaluation and application of modularly assembled zinc-finger nucleases in zebrafish. Development. 2011 Oct;138(20):4555-64. pCS2 (modified) Add to Cart
30702pCS2-F1-GGCF1 GGCmRNA productionEvaluation and application of modularly assembled zinc-finger nucleases in zebrafish. Development. 2011 Oct;138(20):4555-64. pCS2 (modified) Add to Cart
30703pBS-F1-GGGF1 GGGCloning vectorEvaluation and application of modularly assembled zinc-finger nucleases in zebrafish. Development. 2011 Oct;138(20):4555-64. pBlueScript II SK(+) Add to Cart
30704pCS2-F1-GGT-F3-CAGF1 GGT, F3 CAGmRNA productionEvaluation and application of modularly assembled zinc-finger nucleases in zebrafish. Development. 2011 Oct;138(20):4555-64. pCS2 (modified) Add to Cart
30705pBS-F1-GTAF1 GTACloning vectorEvaluation and application of modularly assembled zinc-finger nucleases in zebrafish. Development. 2011 Oct;138(20):4555-64. pBlueScript II SK(+) Add to Cart
30706pBS-F1-GTCF1 GTCCloning vectorEvaluation and application of modularly assembled zinc-finger nucleases in zebrafish. Development. 2011 Oct;138(20):4555-64. pBlueScript II SK(+) Add to Cart
30707pCS2-F1-GTG-F2-GGT-F3-GAAF1 GTG, F2 GGT, F3 GAAmRNA productionEvaluation and application of modularly assembled zinc-finger nucleases in zebrafish. Development. 2011 Oct;138(20):4555-64. pCS2 (modified) Add to Cart
30708pCS2_F1-GTTF1 GTT (Danio rerio)mRNA productionEvaluation and application of modularly assembled zinc-finger nucleases in zebrafish. Development. 2011 Oct;138(20):4555-64. pCS2 (modified) Add to Cart
30709pBS-F1-TAGF1 TAGCloning vectorEvaluation and application of modularly assembled zinc-finger nucleases in zebrafish. Development. 2011 Oct;138(20):4555-64. pBlueScript II SK(+) Add to Cart
30710pCS2-F1-TGG-F3-GGGF1 TGG, F3 GGGmRNA productionEvaluation and application of modularly assembled zinc-finger nucleases in zebrafish. Development. 2011 Oct;138(20):4555-64. pCS2 (modified) Add to Cart
30711pBS-F1-TGTF1 TGT (Danio rerio)Cloning vectorEvaluation and application of modularly assembled zinc-finger nucleases in zebrafish. Development. 2011 Oct;138(20):4555-64. pBlueScript II SK(+) Add to Cart
30712pBS-F1-TTGF1 TTGCloning vectorEvaluation and application of modularly assembled zinc-finger nucleases in zebrafish. Development. 2011 Oct;138(20):4555-64. pBlueScript II SK(+) Add to Cart
30713pBS-F2-AAGF2 AAGCloning vectorEvaluation and application of modularly assembled zinc-finger nucleases in zebrafish. Development. 2011 Oct;138(20):4555-64. pBlueScript II SK(+) Add to Cart
30714pCS2-F2-ACGF2 ACG (Danio rerio)mRNA productionEvaluation and application of modularly assembled zinc-finger nucleases in zebrafish. Development. 2011 Oct;138(20):4555-64. pCS2 (modified) Add to Cart
30715pBS-F2-AGGF2 AGGCloning vectorEvaluation and application of modularly assembled zinc-finger nucleases in zebrafish. Development. 2011 Oct;138(20):4555-64. pBlueScript II SK(+) Add to Cart
30716pBS-F2-ATGF2 ATGCloning vectorEvaluation and application of modularly assembled zinc-finger nucleases in zebrafish. Development. 2011 Oct;138(20):4555-64. pBlueScript II SK(+) Add to Cart
30717pBS-F2-CAGF2 CAG (Danio rerio)Cloning vectorEvaluation and application of modularly assembled zinc-finger nucleases in zebrafish. Development. 2011 Oct;138(20):4555-64. pBlueScript II SK(+) Add to Cart
30718pBS-F2-CGGF2 CGGCloning vectorEvaluation and application of modularly assembled zinc-finger nucleases in zebrafish. Development. 2011 Oct;138(20):4555-64. pBlueScript II SK(+) Add to Cart
30719pBS-F2-GATF2 GATCloning vectorEvaluation and application of modularly assembled zinc-finger nucleases in zebrafish. Development. 2011 Oct;138(20):4555-64. pBlueScript II SK(+) Add to Cart
30720pCS2-F2-GCAF2 GCAmRNA productionEvaluation and application of modularly assembled zinc-finger nucleases in zebrafish. Development. 2011 Oct;138(20):4555-64. pCS2 (modified) Add to Cart
30721pBS-F2-GCCF2 GCCCloning vectorEvaluation and application of modularly assembled zinc-finger nucleases in zebrafish. Development. 2011 Oct;138(20):4555-64. pBlueScript II SK(+) Add to Cart
30722pCS2-F2-GCGF2 GCGmRNA productionEvaluation and application of modularly assembled zinc-finger nucleases in zebrafish. Development. 2011 Oct;138(20):4555-64. pCS2 (modified) Add to Cart
30723pCS2-F2-GCTF2 GCT (Danio rerio)mRNA productionEvaluation and application of modularly assembled zinc-finger nucleases in zebrafish. Development. 2011 Oct;138(20):4555-64. pCS2 Add to Cart
30724pBS-F2-GGAF2 GGACloning vectorEvaluation and application of modularly assembled zinc-finger nucleases in zebrafish. Development. 2011 Oct;138(20):4555-64. pBlueScript II SK(+) Add to Cart
30725pCS2-F2-GGCF2 GGCmRNA productionEvaluation and application of modularly assembled zinc-finger nucleases in zebrafish. Development. 2011 Oct;138(20):4555-64. pCS2 (modified) Add to Cart
30726pBS-F3-GAGF3 GAGCloning vectorEvaluation and application of modularly assembled zinc-finger nucleases in zebrafish. Development. 2011 Oct;138(20):4555-64. pBlueScript II SK(+) Add to Cart
30727pBS-F2-GTAF2 GTACloning vectorEvaluation and application of modularly assembled zinc-finger nucleases in zebrafish. Development. 2011 Oct;138(20):4555-64. pBlueScript II SK(+) Add to Cart
30728pBS-F2-GTCF2 GTCCloning vectorEvaluation and application of modularly assembled zinc-finger nucleases in zebrafish. Development. 2011 Oct;138(20):4555-64. pBlueScript II SK(+) Add to Cart
30729pCS2-F2-GTT-F3-GGCF2 GTT, F3 GGCmRNA productionEvaluation and application of modularly assembled zinc-finger nucleases in zebrafish. Development. 2011 Oct;138(20):4555-64. pCS2 (modified) Add to Cart
30730pBS-F2-TAGF2 TAGCloning vectorEvaluation and application of modularly assembled zinc-finger nucleases in zebrafish. Development. 2011 Oct;138(20):4555-64. pBlueScript II SK(+) Add to Cart
30731pCS2-F2-TGGF2 TGGmRNA productionEvaluation and application of modularly assembled zinc-finger nucleases in zebrafish. Development. 2011 Oct;138(20):4555-64. pCS2 (modified) Add to Cart
30732pBS-F2-TGTF2 TGTCloning vectorEvaluation and application of modularly assembled zinc-finger nucleases in zebrafish. Development. 2011 Oct;138(20):4555-64. pBlueScript II SK(+) Add to Cart
30733pBS-F2-TTGF2 TTGCloning vectorEvaluation and application of modularly assembled zinc-finger nucleases in zebrafish. Development. 2011 Oct;138(20):4555-64. pBlueScript II SK(+) Add to Cart
30734pBS-F3-ACGF3 ACGCloning vectorEvaluation and application of modularly assembled zinc-finger nucleases in zebrafish. Development. 2011 Oct;138(20):4555-64. pBlueScript II SK(+) Add to Cart
30735pBS-F3-AGGF3 AGGCloning vectorEvaluation and application of modularly assembled zinc-finger nucleases in zebrafish. Development. 2011 Oct;138(20):4555-64. pBlueScript II SK(+) Add to Cart
30736pBS-F3-ATGF3 ATGCloning vectorEvaluation and application of modularly assembled zinc-finger nucleases in zebrafish. Development. 2011 Oct;138(20):4555-64. pBlueScript II SK(+) Add to Cart
30737pBS-F3-CGGF3 CGGCloning vectorEvaluation and application of modularly assembled zinc-finger nucleases in zebrafish. Development. 2011 Oct;138(20):4555-64. pBlueScript II SK(+) Add to Cart
30738pBS-F3-GACF3 GACCloning vectorEvaluation and application of modularly assembled zinc-finger nucleases in zebrafish. Development. 2011 Oct;138(20):4555-64. pBlueScript II SK(+) Add to Cart
30739pCS2-F3-GATF3 GATmRNA productionEvaluation and application of modularly assembled zinc-finger nucleases in zebrafish. Development. 2011 Oct;138(20):4555-64. pCS2 (modified) Add to Cart
30740pBS-F3-GCCF3 GCC (Danio rerio)Cloning vectorEvaluation and application of modularly assembled zinc-finger nucleases in zebrafish. Development. 2011 Oct;138(20):4555-64. pBlueScript II SK(+) Add to Cart
30741pCS2-F3-GCTF3 GCTmRNA productionEvaluation and application of modularly assembled zinc-finger nucleases in zebrafish. Development. 2011 Oct;138(20):4555-64. pCS2 (modified) Add to Cart
30742pBS-F3-GGAF3 GGACloning vectorEvaluation and application of modularly assembled zinc-finger nucleases in zebrafish. Development. 2011 Oct;138(20):4555-64. pBlueScript II SK(+) Add to Cart
30743pCS2-F3-GGTF3 GGTmRNA productionEvaluation and application of modularly assembled zinc-finger nucleases in zebrafish. Development. 2011 Oct;138(20):4555-64. pCS2 (modified) Add to Cart
30744pBS-F3-GTAF3 GTACloning vectorEvaluation and application of modularly assembled zinc-finger nucleases in zebrafish. Development. 2011 Oct;138(20):4555-64. pBlueScript II SK(+) Add to Cart
30745pBS-F3-GTCF3 GTCCloning vectorEvaluation and application of modularly assembled zinc-finger nucleases in zebrafish. Development. 2011 Oct;138(20):4555-64. pBlueScript II SK(+) Add to Cart
30746pCS2-F3-GTGF3 GTGmRNA productionEvaluation and application of modularly assembled zinc-finger nucleases in zebrafish. Development. 2011 Oct;138(20):4555-64. pCS2 (modified) Add to Cart
30747pBS-F3-GTTF3 GTTCloning vectorEvaluation and application of modularly assembled zinc-finger nucleases in zebrafish. Development. 2011 Oct;138(20):4555-64. pBlueScript II SK(+) Add to Cart
30748pBS-F3-TAGF3 TAGCloning vectorEvaluation and application of modularly assembled zinc-finger nucleases in zebrafish. Development. 2011 Oct;138(20):4555-64. pBlueScript II SK(+) Add to Cart
30749pBS-F3-TGGF3 TGGCloning vectorEvaluation and application of modularly assembled zinc-finger nucleases in zebrafish. Development. 2011 Oct;138(20):4555-64. pBlueScript II SK(+) Add to Cart
30750pCS2-F3-TGTF3 TGTmRNA productionEvaluation and application of modularly assembled zinc-finger nucleases in zebrafish. Development. 2011 Oct;138(20):4555-64. pCS2 (modified) Add to Cart
30751pBS-F3-GCGF3 GCG (Danio rerio)Cloning vectorEvaluation and application of modularly assembled zinc-finger nucleases in zebrafish. Development. 2011 Oct;138(20):4555-64. pBlueScript II SK(+) Add to Cart
30752pCS2-F2-GGGF2 GGGmRNA productionEvaluation and application of modularly assembled zinc-finger nucleases in zebrafish. Development. 2011 Oct;138(20):4555-64. pCS2 (modified) Add to Cart
30753pBS-F1-GATF1 GAT (Danio rerio)Cloning vectorEvaluation and application of modularly assembled zinc-finger nucleases in zebrafish. Development. 2011 Oct;138(20):4555-64. pBlueScript II SK(+) Add to Cart
30754pCS2-F3-GCAF3 GCAmRNA productionEvaluation and application of modularly assembled zinc-finger nucleases in zebrafish. Development. 2011 Oct;138(20):4555-64. pCS2 (modified) Add to Cart
30755pCS2-F1-AGAF1 AGAmRNA productionEvaluation and application of modularly assembled zinc-finger nucleases in zebrafish. Development. 2011 Oct;138(20):4555-64. pCS2 (modified) Add to Cart
30756pBS-F2-AGAF2 AGA (Danio rerio)Cloning vectorEvaluation and application of modularly assembled zinc-finger nucleases in zebrafish. Development. 2011 Oct;138(20):4555-64. pBlueScript II SK(+) Add to Cart
30757pBS-F3-AGAF3 AGA (Danio rerio)Cloning vectorEvaluation and application of modularly assembled zinc-finger nucleases in zebrafish. Development. 2011 Oct;138(20):4555-64. pBlueScript II SK(+) Add to Cart
35684pCS2-Flag-Kdrl-5p-FokI-ELKdrl-5p-FokI-EL (Danio rerio)mRNA productionEvaluation and application of modularly assembled zinc-finger nucleases in zebrafish. Development. 2011 Oct;138(20):4555-64. pCS2
  • Tag / Fusion Protein
    • Flag (N terminal on backbone)
  • Add to Cart
    35685pCS2-HA-Kdrl-3p-FokI-KKKdrl-3p-FokI-KK (Danio rerio)mRNA productionEvaluation and application of modularly assembled zinc-finger nucleases in zebrafish. Development. 2011 Oct;138(20):4555-64. pCS2
  • Tag / Fusion Protein
    • HA (N terminal on backbone)
  • Add to Cart
    50915pHAGE TRE dCas9dCas9 (Other)Mammalian Expression, Lentiviral, CRISPRCas9 effector-mediated regulation of transcription and differentiation in human pluripotent stem cells. Development. 2014 Jan;141(1):219-23. doi: 10.1242/dev.103341. Tet-regulatable dCas9 lentiviral expression vector pHAGE Add to Cart
    50916pHAGE TRE dCas9-VP64dCas9 (Other)Mammalian Expression, Lentiviral, CRISPRCas9 effector-mediated regulation of transcription and differentiation in human pluripotent stem cells. Development. 2014 Jan;141(1):219-23. doi: 10.1242/dev.103341. Tet-regulatable dCas9-VP64 lentiviral expression vector pHAGE Add to Cart
    50917pHAGE TRE dCas9-KRABdCas9 (Other)Mammalian Expression, Lentiviral, CRISPRCas9 effector-mediated regulation of transcription and differentiation in human pluripotent stem cells. Development. 2014 Jan;141(1):219-23. doi: 10.1242/dev.103341. Tet-regulatable dCas9-KRAB lentiviral expression vector pHAGE Add to Cart
    50918pHAGE EF1α dCas9-VP64dCas9 (Other)Mammalian Expression, Lentiviral, CRISPRCas9 effector-mediated regulation of transcription and differentiation in human pluripotent stem cells. Development. 2014 Jan;141(1):219-23. doi: 10.1242/dev.103341. Constitutive dCas9-VP64 lentiviral expression vector pHAGE Add to Cart
    50919pHAGE EF1α dCas9-KRABdCas9 (Other)Mammalian Expression, Lentiviral, CRISPRCas9 effector-mediated regulation of transcription and differentiation in human pluripotent stem cells. Development. 2014 Jan;141(1):219-23. doi: 10.1242/dev.103341. Constitutive dCas9-KRAB lentiviral expression vector pHAGE Add to Cart
    50920pLKO.1-puro U6 sgRNA BfuAI stufferMammalian Expression, Lentiviral, CRISPRCas9 effector-mediated regulation of transcription and differentiation in human pluripotent stem cells. Development. 2014 Jan;141(1):219-23. doi: 10.1242/dev.103341. U6 driven sgRNA cloning vector where guide sequences are inserted between BfuAI sites pLKO.1 Add to Cart
    50921pLKO.1-puro U6 sgRNA Oct4A -12Oct4A -12 sgRNA (Homo sapiens)Mammalian Expression, Lentiviral, CRISPRCas9 effector-mediated regulation of transcription and differentiation in human pluripotent stem cells. Development. 2014 Jan;141(1):219-23. doi: 10.1242/dev.103341. U6 driven sgRNA targeting OCT4 isoform A -12 bp from TSS pLKO.1 POU5F1 OCT3, OCT4, OTF-3, OTF3, OTF4, Oct-3, Oct-4 Add to Cart
    50922pLKO.1-puro U6 sgRNA Oct4A -158Oct4A -158 sgRNA (Homo sapiens)Mammalian Expression, Lentiviral, CRISPRCas9 effector-mediated regulation of transcription and differentiation in human pluripotent stem cells. Development. 2014 Jan;141(1):219-23. doi: 10.1242/dev.103341. U6 driven sgRNA targeting OCT4 isoform A -158 bp from TSS pLKO.1 POU5F1 OCT3, OCT4, OTF-3, OTF3, OTF4, Oct-3, Oct-4 Add to Cart
    50923pLKO.1-puro U6 sgRNA SOX17 -91Sox17 -91 sgRNA (Homo sapiens)Mammalian Expression, Lentiviral, CRISPRCas9 effector-mediated regulation of transcription and differentiation in human pluripotent stem cells. Development. 2014 Jan;141(1):219-23. doi: 10.1242/dev.103341. U6 driven sgRNA targeting Sox17 -91 bp from TSS pLKO.1 SOX17 VUR3 Add to Cart
    50924pLKO.1-puro U6 sgRNA SOX17 -126Sox17 -126 sgRNA (Homo sapiens)Mammalian Expression, Lentiviral, CRISPRCas9 effector-mediated regulation of transcription and differentiation in human pluripotent stem cells. Development. 2014 Jan;141(1):219-23. doi: 10.1242/dev.103341. U6 driven sgRNA targeting Sox17 -126 bp from TSS pLKO.1 SOX17 VUR3 Add to Cart
    50925pLKO.1-puro U6 sgRNA SOX17 -177Sox17 -177 sgRNA (Homo sapiens)Mammalian Expression, Lentiviral, CRISPRCas9 effector-mediated regulation of transcription and differentiation in human pluripotent stem cells. Development. 2014 Jan;141(1):219-23. doi: 10.1242/dev.103341. U6 driven sgRNA targeting Sox17 -177 bp from TSS pLKO.1 SOX17 VUR3 Add to Cart
    50926pLKO.1-puro U6 sgRNA SOX17 -296Sox17-296 sgRNA (Homo sapiens)Mammalian Expression, Lentiviral, CRISPRCas9 effector-mediated regulation of transcription and differentiation in human pluripotent stem cells. Development. 2014 Jan;141(1):219-23. doi: 10.1242/dev.103341. U6 driven sgRNA targeting Sox17 -296 bp from TSS pLKO.1 SOX17 VUR3 Add to Cart
    50927pLKO.1-puro U6 sgRNA CAGnegative control sgRNA (Other)Mammalian Expression, Lentiviral, CRISPRCas9 effector-mediated regulation of transcription and differentiation in human pluripotent stem cells. Development. 2014 Jan;141(1):219-23. doi: 10.1242/dev.103341. U6 driven sgRNA negative control pLKO.1 Add to Cart
    52628pLKO.1-puro U6 sgRNA BfuAI large stufferKanamycin cassette (Other)Mammalian Expression, Lentiviral, CRISPR Improved U6-sgRNA vector for expression (unpublished) lentiviral U6 driven sgRNA cloning vector where guide sequences are inserted between BfuAI sites, improved cassette cloning efficiency pLKO.1 Add to Cart
    69219pCSDest2-SpCas9-NLS-3XHA-NLS-Zif268Zif268 (Mus musculus)Mammalian Expression, CRISPRDNA-binding-domain fusions enhance the targeting range and precision of Cas9. Nat Methods. 2015 Oct 19. doi: 10.1038/nmeth.3624. Expresses wild type SpCas9 fused to Zif268 in mammalian cells pCSDest2-SpCas9-NLS-3XHA-NLS Egr1 A530045N19Rik, ETR103, Egr-1, Krox-1, Krox-24, Krox24, NGF1-A, NGFI-A, NGFIA, TIS8, Zenk, Zfp-6, Zif268, egr Add to Cart
    69220pCSDest2-SpCas9-NLS-3XHA-NLSSpCas9 (Other)Mammalian Expression, CRISPRDNA-binding-domain fusions enhance the targeting range and precision of Cas9. Nat Methods. 2015 Oct 19. doi: 10.1038/nmeth.3624. Expresses wild type SpCas9 in mammalian cells pCSDest2 Add to Cart
    69221pCSDest2-SpCas9-MT1-NLS-3XHA-NLSSpCas9 (Other)Mammalian Expression, CRISPRDNA-binding-domain fusions enhance the targeting range and precision of Cas9. Nat Methods. 2015 Oct 19. doi: 10.1038/nmeth.3624. Expresses R1333K mutant SpCas9 in mammalian cell pCSDest2-SpCas9-NLS-3XHA-NLS Add to Cart
    69222pCSDest2-SpCas9-MT2-NLS-3XHA-NLSSpCas9 (Other)Mammalian Expression, CRISPRDNA-binding-domain fusions enhance the targeting range and precision of Cas9. Nat Methods. 2015 Oct 19. doi: 10.1038/nmeth.3624. Expresses R1333S mutant SpCas9 in mammalian cell pCSDest2-SpCas9-NLS-3XHA-NLS Add to Cart
    69223pCSDest2-SpCas9-MT3-NLS-3XHA-NLSSpCas9 (Other)Mammalian Expression, CRISPRDNA-binding-domain fusions enhance the targeting range and precision of Cas9. Nat Methods. 2015 Oct 19. doi: 10.1038/nmeth.3624. Expresses R1335K mutant SpCas9 in mammalian cell pCSDest2-SpCas9-NLS-3XHA-NLS Add to Cart
    69224pCSDest2-SpCas9-MT3-NLS-3XHA-NLS-Zif268SpCas9 (Other)Mammalian Expression, CRISPRDNA-binding-domain fusions enhance the targeting range and precision of Cas9. Nat Methods. 2015 Oct 19. doi: 10.1038/nmeth.3624. Expresses R1335K mutant SpCas9 fused to Zif268 in mammalian cell pCSDest2-SpCas9-NLS-3XHA-NLS-Zif268 Add to Cart
    69225pCSDest2-SpCas9-WT-NLS-3XHA-NLS-ZFP_TS2TS2 ZFP (Synthetic)Mammalian Expression, CRISPRDNA-binding-domain fusions enhance the targeting range and precision of Cas9. Nat Methods. 2015 Oct 19. doi: 10.1038/nmeth.3624. Expresses wild type SpCas9 fused to ZFP-TS2 in mammalian cells pCSDest2-SpCas9-NLS-3XHA-NLS Add to Cart
    69226pCSDest2-SpCas9-WT-NLS-3XHA-NLS-ZFP_TS3TS3 ZFP (Synthetic)Mammalian Expression, CRISPRDNA-binding-domain fusions enhance the targeting range and precision of Cas9. Nat Methods. 2015 Oct 19. doi: 10.1038/nmeth.3624. Expresses wild type SpCas9 fused to ZFP-TS3 in mammalian cells pCSDest2-SpCas9-NLS-3XHA-NLS Add to Cart
    69227pCSDest2-SpCas9-WT-NLS-3XHA-NLS-ZFP_TS4TS4 ZFP (Synthetic)Mammalian Expression, CRISPRDNA-binding-domain fusions enhance the targeting range and precision of Cas9. Nat Methods. 2015 Oct 19. doi: 10.1038/nmeth.3624. Expresses wild type SpCas9 fused to ZFP-TS4 in mammalian cells pCSDest2-SpCas9-NLS-3XHA-NLS Add to Cart
    69228pCSDest2-SpCas9-MT3-NLS-3XHA-NLS-ZFP_TS2TS2 ZFP (Synthetic)Mammalian Expression, CRISPRDNA-binding-domain fusions enhance the targeting range and precision of Cas9. Nat Methods. 2015 Oct 19. doi: 10.1038/nmeth.3624. Expresses R1335K mutant SpCas9 fused to ZFP-TS2 in mammalian cell pCSDest2-SpCas9-MT3-NLS-3XHA-NLS-Zif268 Add to Cart
    69229pCSDest2-SpCas9-MT3-NLS-3XHA-NLS-ZFP_TS3TS3 ZFP (Synthetic)Mammalian Expression, CRISPRDNA-binding-domain fusions enhance the targeting range and precision of Cas9. Nat Methods. 2015 Oct 19. doi: 10.1038/nmeth.3624. Expresses R1335K mutant SpCas9 fused to ZFP-TS3 in mammalian cell pCSDest2-SpCas9-MT3-NLS-3XHA-NLS-Zif268 Add to Cart
    69230pCSDest2-SpCas9-MT3-NLS-3XHA-NLS-ZFP_TS4TS4 ZFP (Synthetic)Mammalian Expression, CRISPRDNA-binding-domain fusions enhance the targeting range and precision of Cas9. Nat Methods. 2015 Oct 19. doi: 10.1038/nmeth.3624. Expresses R1335K mutant SpCas9 fused to ZFP-TS4 in mammalian cell pCSDest2-SpCas9-MT3-NLS-3XHA-NLS-Zif268 Add to Cart
    69231pCSDest2-2XNLS-SpCas9-MT3-NLS-3XHA-NLS-TALE_TS3TS3 TALE (Synthetic)Mammalian Expression, CRISPRDNA-binding-domain fusions enhance the targeting range and precision of Cas9. Nat Methods. 2015 Oct 19. doi: 10.1038/nmeth.3624. Expresses R1335K mutant SpCas9 fused to TALE-TS3 in mammalian cell pCSDest2-2XNLS-SpCas9-MT3-NLS-3XHA-NLS-TALentry Add to Cart
    69232pCSDest2-2XNLS-SpCas9-WT-NLS-3XHA-NLS-TALentrySpCas9 (Other)Mammalian Expression, CRISPRDNA-binding-domain fusions enhance the targeting range and precision of Cas9. Nat Methods. 2015 Oct 19. doi: 10.1038/nmeth.3624. Wild type SpCas9 expression plasmid to clone TALEs through BbsI site (generates compatible overhangs with Acc65I and BamHI sites) pCSDest2 Add to Cart
    69233pCSDest2-2XNLS-SpCas9-MT3-NLS-3XHA-NLS-TALentrySpCas9 (Other)Mammalian Expression, CRISPRDNA-binding-domain fusions enhance the targeting range and precision of Cas9. Nat Methods. 2015 Oct 19. doi: 10.1038/nmeth.3624. R1335K mutant SpCas9 expression plasmid to clone TALEs through BbsI site (generates compatible overhangs with Acc65I and BamHI sites) pCSDest2 Add to Cart
    69234pLKO1-puro-U6-sgRNA-TS2TS2 sgRNA (Homo sapiens)Mammalian Expression, Lentiviral, CRISPRDNA-binding-domain fusions enhance the targeting range and precision of Cas9. Nat Methods. 2015 Oct 19. doi: 10.1038/nmeth.3624. U6 driven SpCas9 sgRNA expression for TS2 site pLKO.1 Add to Cart
    69235pLKO1-puro-U6-sgRNA-TS3TS3 sgRNA (Homo sapiens)Mammalian Expression, Lentiviral, CRISPRDNA-binding-domain fusions enhance the targeting range and precision of Cas9. Nat Methods. 2015 Oct 19. doi: 10.1038/nmeth.3624. U6 driven SpCas9 sgRNA expression for TS3 site pLKO.1 Add to Cart
    69236pLKO1-puro-U6-sgRNA-TS4TS4 sgRNA (Homo sapiens)Mammalian Expression, Lentiviral, CRISPRDNA-binding-domain fusions enhance the targeting range and precision of Cas9. Nat Methods. 2015 Oct 19. doi: 10.1038/nmeth.3624. U6 driven SpCas9 sgRNA expression for TS4 site pLKO.1 Add to Cart
    69237pLKO1-puro-U6-sgRNA-LRTM2LRTM2 sgRNA (Homo sapiens)Mammalian Expression, Lentiviral, CRISPRDNA-binding-domain fusions enhance the targeting range and precision of Cas9. Nat Methods. 2015 Oct 19. doi: 10.1038/nmeth.3624. U6 driven SpCas9 sgRNA expression for LRTM2 site pLKO.1 LRTM2 Add to Cart
    69238pLKO1-puro-U6-sgRNA-KANK3KANK3 sgRNA (Homo sapiens)Mammalian Expression, Lentiviral, CRISPRDNA-binding-domain fusions enhance the targeting range and precision of Cas9. Nat Methods. 2015 Oct 19. doi: 10.1038/nmeth.3624. U6 driven SpCas9 sgRNA expression for KANK3 site pLKO.1 KANK3 ANKRD47 Add to Cart
    69239pLKO1-puro-U6-sgRNA-TGM2TGM2 sgRNA (Homo sapiens)Mammalian Expression, Lentiviral, CRISPRDNA-binding-domain fusions enhance the targeting range and precision of Cas9. Nat Methods. 2015 Oct 19. doi: 10.1038/nmeth.3624. U6 driven SpCas9 sgRNA expression for TGM2 site pLKO.1 TGM2 TG(C), TGC Add to Cart
    69240pLKO1-puro-U6-sgRNA-PLXNB2PLXNB2 sgRNA (Homo sapiens)Mammalian Expression, Lentiviral, CRISPRDNA-binding-domain fusions enhance the targeting range and precision of Cas9. Nat Methods. 2015 Oct 19. doi: 10.1038/nmeth.3624. U6 driven SpCas9 sgRNA expression for PLXNB2 site pLKO.1 PLXNB2 MM1, Nbla00445, PLEXB2, dJ402G11.3 Add to Cart
    86195pLKO.1-puro U6 N. meningitidis sgRNA BfuAI large stuffernmCas9 sgRNA cloning cassete (Other)Lentiviral pLKO.1-puro U6 N. meningitidis sgRNA BfuAI large stuffer (unpublished) U6 based expression of N meningitidis sgRNA pLKO.1 Add to Cart