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Sebastian Lourido Lab Plasmids


The Sebastian Lourido Lab has deposited plasmids at Addgene for distribution to the research community. Addgene is a nonprofit plasmid repository dedicated to improving life science research.

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Title Authors Publication
Lourido lab Toxoplasma CRISPR Plasmid (unpublished)
Using a Genetically Encoded Sensor to Identify Inhibitors of Toxoplasma gondii Ca2+ Signaling. Sidik SM, Hortua Triana MA, Paul AS, El Bakkouri M, Hackett CG, Tran F, Westwood NJ, Hui R, Zuercher WJ, Duraisingh MT, Moreno SN, Lourido S J Biol Chem. 2016 Apr 29;291(18):9566-80. doi: 10.1074/jbc.M115.703546. Epub 2016 Mar 1.
A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik SM, Huet D, Ganesan SM, Huynh MH, Wang T, Nasamu AS, Thiru P, Saeij JP, Carruthers VB, Niles JC, Lourido S Cell. 2016 Sep 8;166(6):1423-1435.e12. doi: 10.1016/j.cell.2016.08.019. Epub 2016 Sep 2.
ID Plasmid Gene/Insert Vector Type Publication Hidden Extra Search Info  
52694pU6-UniversalCas9 (Synthetic), Toxoplasma U6 upstream region - protospacer cloning sequence - chiRNA (Synthetic)CRISPR ; Toxoplasma gondii Lourido lab Toxoplasma CRISPR Plasmid (unpublished) This plasmid can be used to mutate genes in Toxoplasma gondii using the CRISPR/Cas9 system after having an appropriate protospacer cloned into it. pX330 Add to Cart
78705pGCaMP5GCaMP5 (Synthetic)Using a Genetically Encoded Sensor to Identify Inhibitors of Toxoplasma gondii Ca2+ Signaling. J Biol Chem. 2016 Apr 29;291(18):9566-80. doi: 10.1074/jbc.M115.703546. Epub 2016 Mar 1. GCaMP5 under the Toxoplasma SAG1 5' UTR. No Toxoplasma selectable marker N/A Add to Cart
78706pGCaMP5-DHFRGCaMP5 (Synthetic), DHFR (Other)Using a Genetically Encoded Sensor to Identify Inhibitors of Toxoplasma gondii Ca2+ Signaling. J Biol Chem. 2016 Apr 29;291(18):9566-80. doi: 10.1074/jbc.M115.703546. Epub 2016 Mar 1. GCaMP5 under the Toxoplasma SAG1 5' UTR. Encodes a DHFR selectable marker. N/A Add to Cart
80322pU6-SAG1-DHFRSAG1 sgRNACRISPRA Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Cell. 2016 Sep 8;166(6):1423-1435.e12. doi: 10.1016/j.cell.2016.08.019. Epub 2016 Sep 2. Encodes a CRISPR sgRNA that allows for mutation of the surface antigen SAG1 in Toxoplasma gondii and confers resistance to pyrimethamine pU6-DHFR Add to Cart
80323pCas9-CATCas9 (Other), Chloramphenicol acetyltransferaseCRISPRA Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Cell. 2016 Sep 8;166(6):1423-1435.e12. doi: 10.1016/j.cell.2016.08.019. Epub 2016 Sep 2. Encodes Cas9 and chloramphenicol acetyltransferase (CAT) pU6-Universal Add to Cart
80324pU6-DecoyDecoy sgRNACRISPRA Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Cell. 2016 Sep 8;166(6):1423-1435.e12. doi: 10.1016/j.cell.2016.08.019. Epub 2016 Sep 2. Encodes Cas9 and a CRISPR sgRNA that alleviates toxicity to Cas9 in Toxoplasma gondii pU6-Universal Add to Cart
80329pU6-DHFRDHFR-TSc3 (Other)CRISPRA Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Cell. 2016 Sep 8;166(6):1423-1435.e12. doi: 10.1016/j.cell.2016.08.019. Epub 2016 Sep 2. Allows for expression of an sgRNA in Toxoplasma gondii and confers resistance to pyrimethamine pU6-Universal Add to Cart
80636Lourido Toxoplasma CRISPR Library V1~80,000 sgRNAs, ten each against each of the 8,158 predicted Toxoplasma gondii protein coding genesA Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Cell. 2016 Sep 8;166(6):1423-1435.e12. doi: 10.1016/j.cell.2016.08.019. Epub 2016 Sep 2. Plasmid sgRNA library containing ten guides against each of the 8,158 predicted Toxoplasma gondii protein coding genes. pU6-DHFR Add to Cart