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Simon Bullock Lab Plasmids


The Simon Bullock Lab has deposited plasmids at Addgene for distribution to the research community. Addgene is a nonprofit plasmid repository dedicated to improving life science research.

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Title Authors Publication
Optimized CRISPR/Cas tools for efficient germline and somatic genome engineering in Drosophila. Port F, Chen HM, Lee T, Bullock SL Proc Natl Acad Sci U S A. 2014 Jul 7. pii: 201405500.
CRISPRflydesign (unpublished)
Augmenting CRISPR applications in Drosophila with tRNA-flanked sgRNAs. Port F, Bullock SL Nat Methods. 2016 Oct;13(10):852-4. doi: 10.1038/nmeth.3972. Epub 2016 Sep 5.
ID Plasmid Gene/Insert Vector Type Publication Hidden Extra Search Info  
49408pCFD1-dU6:1gRNAdU6-1:gRNA (Drosophila melanogaster)Insect Expression, CRISPROptimized CRISPR/Cas tools for efficient germline and somatic genome engineering in Drosophila. Proc Natl Acad Sci U S A. 2014 Jul 7. pii: 201405500. expression of gRNA under control of the Drosophila U6:1 promoter pValium22 Add to Cart
49409pCFD2-dU6:2gRNAdU6-2:gRNA (Drosophila melanogaster)Insect Expression, CRISPROptimized CRISPR/Cas tools for efficient germline and somatic genome engineering in Drosophila. Proc Natl Acad Sci U S A. 2014 Jul 7. pii: 201405500. expression of gRNA under control of the Drosophila U6:2 promoter pValium22 Add to Cart
49410pCFD3-dU6:3gRNAdU6-3:gRNA (Drosophila melanogaster)Insect Expression, CRISPROptimized CRISPR/Cas tools for efficient germline and somatic genome engineering in Drosophila. Proc Natl Acad Sci U S A. 2014 Jul 7. pii: 201405500. expression of gRNA under control of the Drosophila U6:3 promoter pValium22 Add to Cart
49411pCFD4-U6:1_U6:3tandemgRNAsdU6-1:gRNA (Drosophila melanogaster), dU6-3:gRNA (Drosophila melanogaster)Insect Expression, CRISPROptimized CRISPR/Cas tools for efficient germline and somatic genome engineering in Drosophila. Proc Natl Acad Sci U S A. 2014 Jul 7. pii: 201405500. expression of two gRNAs from Drosophila U6:1 and U6:3 pValium22 Add to Cart
62208pnos-Cas9-noscas9Insect ExpressionOptimized CRISPR/Cas tools for efficient germline and somatic genome engineering in Drosophila. Proc Natl Acad Sci U S A. 2014 Jul 7. pii: 201405500. Expresses Cas9 under control of nanos promoter and 3'UTR. For germ line restricted genome engineering in Drosophila melanogaster. pnos Add to Cart
62209pAct-Cas9cas9Insect ExpressionOptimized CRISPR/Cas tools for efficient germline and somatic genome engineering in Drosophila. Proc Natl Acad Sci U S A. 2014 Jul 7. pii: 201405500. Expresses Cas9 under control of act5C promoter and SV40 3'UTR. For ubiquitous genome engineering in Drosophila melanogaster. pact Add to Cart
62210pnos-Fok1:dCas9-nosFok1-dCas9Insect Expression CRISPRflydesign (unpublished) Expresses Fok1:dCas9 under control of nanos promoter and 3'UTR. For germ line restricted high specificity genome engineering in Drosophila melanogaster. pnos Add to Cart
62211pAct-Fok1:dCas9Fok1-dCas9Insect Expression CRISPRflydesign (unpublished) Expresses Fok1:dCas9 under control of act5C promoter and SV40 3'UTR. For ubiquitous high specificity genome engineering in Drosophila melanogaster. pact Add to Cart
73914pCFD5dU6-3:tRNA:gRNA:tRNA:gRNA (Drosophila melanogaster)Insect Expression, CRISPRAugmenting CRISPR applications in Drosophila with tRNA-flanked sgRNAs. Nat Methods. 2016 Oct;13(10):852-4. doi: 10.1038/nmeth.3972. Epub 2016 Sep 5. multiplex gRNA plasmid pValium22 Add to Cart
73915pCFD6:tRNA:gRNA:tRNA:gRNA:tRNA (Drosophila melanogaster)Insect Expression, CRISPRAugmenting CRISPR applications in Drosophila with tRNA-flanked sgRNAs. Nat Methods. 2016 Oct;13(10):852-4. doi: 10.1038/nmeth.3972. Epub 2016 Sep 5. tissue-specific multiplex gRNA plasmid pBIC-UASC Add to Cart
73916pCFD7tRNA:hAsCpf1-gRNA:tRNA (Homo sapiens)Insect Expression, CRISPRAugmenting CRISPR applications in Drosophila with tRNA-flanked sgRNAs. Nat Methods. 2016 Oct;13(10):852-4. doi: 10.1038/nmeth.3972. Epub 2016 Sep 5. gRNA vector for AsCpf1 pValium22 Add to Cart
73917act-AsCpf1hAsCpf1 (Homo sapiens)Insect ExpressionAugmenting CRISPR applications in Drosophila with tRNA-flanked sgRNAs. Nat Methods. 2016 Oct;13(10):852-4. doi: 10.1038/nmeth.3972. Epub 2016 Sep 5. expression plasmid of AsCpf1 pact Add to Cart