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Stanley Qi Lab Plasmids


The Stanley Qi Lab has deposited plasmids at Addgene for distribution to the research community. Addgene is a nonprofit plasmid repository dedicated to improving life science research.

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Title Authors Publication
Repurposing CRISPR as an RNA-Guided Platform for Sequence-Specific Control of Gene Expression. Qi LS, Larson MH, Gilbert LA, Doudna JA, Weissman JS, Arkin AP, Lim WA Cell. 2013 Feb 28;152(5):1173-83. doi: 10.1016/j.cell.2013.02.022.
RNA processing enables predictable programming of gene expression. Qi L, Haurwitz RE, Shao W, Doudna JA, Arkin AP Nat Biotechnol. 2012 Oct;30(10):1002-6. doi: 10.1038/nbt.2355. Epub 2012 Sep 16.
CRISPR-Mediated Modular RNA-Guided Regulation of Transcription in Eukaryotes. Gilbert LA, Larson MH, Morsut L, Liu Z, Brar GA, Torres SE, Stern-Ginossar N, Brandman O, Whitehead EH, Doudna JA, Lim WA, Weissman JS, Qi LS Cell. 2013 Jul 9. pii: S0092-8674(13)00826-X. doi: 10.1016/j.cell.2013.06.044.
Dynamic Imaging of Genomic Loci in Living Human Cells by an Optimized CRISPR/Cas System. Chen B, Gilbert LA, Cimini BA, Schnitzbauer J, Zhang W, Li GW, Park J, Blackburn EH, Weissman JS, Qi LS, Huang B Cell. 2013 Dec 19;155(7):1479-91. doi: 10.1016/j.cell.2013.12.001.
Engineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds. Zalatan JG, Lee ME, Almeida R, Gilbert LA, Whitehead EH, La Russa M, Tsai JC, Weissman JS, Dueber JE, Qi LS, Lim WA Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 10.1016/j.cell.2014.11.052.
Small Molecules Enhance CRISPR Genome Editing in Pluripotent Stem Cells. Yu C, Liu Y, Ma T, Liu K, Xu S, Zhang Y, Liu H, La Russa M, Xie M, Ding S, Qi LS Cell Stem Cell. 2015 Feb 5;16(2):142-7. doi: 10.1016/j.stem.2015.01.003.
A Comprehensive, CRISPR-based Functional Analysis of Essential Genes in Bacteria. Peters JM, Colavin A, Shi H, Czarny TL, Larson MH, Wong S, Hawkins JS, Lu CH, Koo BM, Marta E, Shiver AL, Whitehead EH, Weissman JS, Brown ED, Qi LS, Huang KC, Gross CA Cell. 2016 Jun 2;165(6):1493-506. doi: 10.1016/j.cell.2016.05.003. Epub 2016 May 26.
Complex transcriptional modulation with orthogonal and inducible dCas9 regulators. Gao Y, Xiong X, Wong S, Charles EJ, Lim WA, Qi LS Nat Methods. 2016 Oct 24. doi: 10.1038/nmeth.4042.
ID Plasmid Gene/Insert Vector Type Publication Hidden Extra Search Info  
44246pdCas9-humanizeddead Cas9 with 3X NLS (Homo sapiens)Mammalian Expression, Retroviral, CRISPR ; vectorRepurposing CRISPR as an RNA-Guided Platform for Sequence-Specific Control of Gene Expression. Cell. 2013 Feb 28;152(5):1173-83. doi: 10.1016/j.cell.2013.02.022. Expression of a catalytically inactive, human codon-optimized Cas9 under the control of Murine Stem Cell retroVirus LTR promoter for mammalian gene knockdown pMSCVpuro Add to Cart
44247pdCas9::BFP-humanizeddCas9 fused to BFP (Homo sapiens)Mammalian Expression, Retroviral, CRISPRRepurposing CRISPR as an RNA-Guided Platform for Sequence-Specific Control of Gene Expression. Cell. 2013 Feb 28;152(5):1173-83. doi: 10.1016/j.cell.2013.02.022. Expression of a catalytically inactive, human codon-optimized Cas9-BFP fusion under the control of Murine Stem Cell retroVirus LTR promoter for mammalian gene knockdown pMSCVpuro Add to Cart
44248pgRNA-humanizedguide RNA (Homo sapiens)Mammalian Expression, Lentiviral, CRISPRRepurposing CRISPR as an RNA-Guided Platform for Sequence-Specific Control of Gene Expression. Cell. 2013 Feb 28;152(5):1173-83. doi: 10.1016/j.cell.2013.02.022. Expression of customizable guide RNA (gRNA) under cotnrol of murine U6 promoter, also contains a CMV-puro-t2A-mCherry expression cassette for mammalian gene knockdown pU6 Add to Cart
44249pdCas9-bacteriadCas9 (bacteria) (Other)Bacterial Expression, CRISPRRepurposing CRISPR as an RNA-Guided Platform for Sequence-Specific Control of Gene Expression. Cell. 2013 Feb 28;152(5):1173-83. doi: 10.1016/j.cell.2013.02.022. aTc-inducible expression of a catalytically inactive bacterial Cas9 (S. pyogenes) for bacterial gene knockdown p15A vector Add to Cart
44250pwtCas9-bacteriawild-type Cas9Bacterial Expression, CRISPRRepurposing CRISPR as an RNA-Guided Platform for Sequence-Specific Control of Gene Expression. Cell. 2013 Feb 28;152(5):1173-83. doi: 10.1016/j.cell.2013.02.022. aTc-inducible expression of wild-type Cas9 (S .pyogenes) for bacterial gene knockdown pUC19 Add to Cart
44251pgRNA-bacteriaguide RNA (bacteria)Bacterial Expression, CRISPRRepurposing CRISPR as an RNA-Guided Platform for Sequence-Specific Control of Gene Expression. Cell. 2013 Feb 28;152(5):1173-83. doi: 10.1016/j.cell.2013.02.022. Expression of customizable guide RNA (gRNA) for bacterial gene knockdown pUC19 Add to Cart
44252pCsy4csy4 geneBacterial Expression, Synthetic BiologyRNA processing enables predictable programming of gene expression. Nat Biotechnol. 2012 Oct;30(10):1002-6. doi: 10.1038/nbt.2355. Epub 2012 Sep 16. pUC19 Add to Cart
46910pHR-SFFV-dCas9-BFPdCas9-BFP fusion (Homo sapiens)Mammalian Expression, Lentiviral, CRISPRCRISPR-Mediated Modular RNA-Guided Regulation of Transcription in Eukaryotes. Cell. 2013 Jul 9. pii: S0092-8674(13)00826-X. doi: 10.1016/j.cell.2013.06.044. Human expression vector containing SFFV promoter, dCas9 that is fused to 2x NLS and tagBFP pHR Add to Cart
46911pHR-SFFV-dCas9-BFP-KRABdCas9-BFP-KRAB fusion (Homo sapiens)Mammalian Expression, Lentiviral, CRISPRCRISPR-Mediated Modular RNA-Guided Regulation of Transcription in Eukaryotes. Cell. 2013 Jul 9. pii: S0092-8674(13)00826-X. doi: 10.1016/j.cell.2013.06.044. Human expression vector containing SFFV promoter, dCas9 that is fused to 2x NLS, tagBFP and a KRAB domain pHR Add to Cart
46912pMSCV-LTR-dCas9-VP64-BFPdCas9-VP64-BFP fusion (Homo sapiens), Puromycin resistanceMammalian Expression, Retroviral, CRISPRCRISPR-Mediated Modular RNA-Guided Regulation of Transcription in Eukaryotes. Cell. 2013 Jul 9. pii: S0092-8674(13)00826-X. doi: 10.1016/j.cell.2013.06.044. Human expression vector containing MSCV LTR promoter, dCas9 that is fused to 2x NLS, VP64 and tagBFP MSCV-puro Add to Cart
46913pMSCV-LTR-dCas9-p65AD-BFPdCas9-p65AD-BFP fusion (Homo sapiens), Puromycin resistanceMammalian Expression, Retroviral, CRISPRCRISPR-Mediated Modular RNA-Guided Regulation of Transcription in Eukaryotes. Cell. 2013 Jul 9. pii: S0092-8674(13)00826-X. doi: 10.1016/j.cell.2013.06.044. Human expression vector containing MSCV LTR promoter, dCas9 that is fused to 2x NLS, p65 activation domain and tagBFP MSCV-puro Add to Cart
46914pU6-sgGFP-NT1sgGFP-NT1Mammalian Expression, Lentiviral, CRISPRCRISPR-Mediated Modular RNA-Guided Regulation of Transcription in Eukaryotes. Cell. 2013 Jul 9. pii: S0092-8674(13)00826-X. doi: 10.1016/j.cell.2013.06.044. Human pSico-based U6 vector containing murine U6 promoter and sgRNA targeting GFP (NT1) pSICO derivative Add to Cart
46915pU6-sgGAL4-1sgGAL4-1, Puromycin resistance and mCherryMammalian Expression, Lentiviral, CRISPRCRISPR-Mediated Modular RNA-Guided Regulation of Transcription in Eukaryotes. Cell. 2013 Jul 9. pii: S0092-8674(13)00826-X. doi: 10.1016/j.cell.2013.06.044. Human pSico-based U6 vector containing murine U6 promoter and sgRNA targeting GAL4 UAS promoter pSICO derivative Add to Cart
46916pU6-sgGAL4-4sgGAL4-4, Puromycin resistance and mCherryMammalian Expression, Lentiviral, CRISPRCRISPR-Mediated Modular RNA-Guided Regulation of Transcription in Eukaryotes. Cell. 2013 Jul 9. pii: S0092-8674(13)00826-X. doi: 10.1016/j.cell.2013.06.044. Human pSico-based U6 vector containing murine U6 promoter and sgRNA targeting GAL4 UAS promoter (negative control) pSICO derivative Add to Cart
46917pU6-sgCXCR4-2sgCXCR4 -2 (Homo sapiens), Puromycin resistance and mCherryMammalian Expression, Lentiviral, CRISPRCRISPR-Mediated Modular RNA-Guided Regulation of Transcription in Eukaryotes. Cell. 2013 Jul 9. pii: S0092-8674(13)00826-X. doi: 10.1016/j.cell.2013.06.044. Human pSico-based U6 vector containing murine U6 promoter and sgRNA targeting endogenous CXCR4 gene pSICO derivative CXCR4 CD184, D2S201E, FB22, HM89, HSY3RR, LAP-3, LAP3, LCR1, LESTR, NPY3R, NPYR, NPYRL, NPYY3R, WHIM, WHIMS Add to Cart
46918pU6-sgCD71-2sgCD71 -2 (Homo sapiens), Puromycin resistance and mCherryMammalian Expression, Lentiviral, CRISPRCRISPR-Mediated Modular RNA-Guided Regulation of Transcription in Eukaryotes. Cell. 2013 Jul 9. pii: S0092-8674(13)00826-X. doi: 10.1016/j.cell.2013.06.044. Human pSico-based U6 vector containing murine U6 promoter and sgRNA targeting endogenous CD71 gene pSICO derivative TFRC CD71, IMD46, T9, TFR, TFR1, TR, TRFR, p90 Add to Cart
46919pMLS-SV40-EGFPGFPMammalian Expression, Retroviral, CRISPRCRISPR-Mediated Modular RNA-Guided Regulation of Transcription in Eukaryotes. Cell. 2013 Jul 9. pii: S0092-8674(13)00826-X. doi: 10.1016/j.cell.2013.06.044. Target EGFP gene that is stably integrated into HEK293 genome MSCV-puro Add to Cart
46920pTDH3-dCas9dCas9Yeast Expression, CRISPRCRISPR-Mediated Modular RNA-Guided Regulation of Transcription in Eukaryotes. Cell. 2013 Jul 9. pii: S0092-8674(13)00826-X. doi: 10.1016/j.cell.2013.06.044. Yeast CEN/ARS vector (Leu2) that contains dCas9 fused to NLS controlled by TDH3 promoter pJED103 Add to Cart
46921pTDH3-dCas9-Mxi1dCas9-Mxi1Yeast Expression, CRISPRCRISPR-Mediated Modular RNA-Guided Regulation of Transcription in Eukaryotes. Cell. 2013 Jul 9. pii: S0092-8674(13)00826-X. doi: 10.1016/j.cell.2013.06.044. Yeast CEN/ARS vector (Leu2) that contains dCas9 fused to NLS and Mxi1 domain controlled by TDH3 promoter pJED103 Add to Cart
46922pSNR52-sgTEF1sgTEF1 promoter (Saccharomyces cerevisiae)Yeast Expression, CRISPRCRISPR-Mediated Modular RNA-Guided Regulation of Transcription in Eukaryotes. Cell. 2013 Jul 9. pii: S0092-8674(13)00826-X. doi: 10.1016/j.cell.2013.06.044. Yeast CEN/ARS vector (Ura3) that contains sgRNA controlled by SNR 52 promoter, targeting endogenous TEF1 promoter AH057 TEF1 YPR080W Add to Cart
46923pSNR52-sgTETsgRNA targeting endogenous TRE elements of pTET07 promoter (Saccharomyces cerevisiae)Yeast Expression, CRISPRCRISPR-Mediated Modular RNA-Guided Regulation of Transcription in Eukaryotes. Cell. 2013 Jul 9. pii: S0092-8674(13)00826-X. doi: 10.1016/j.cell.2013.06.044. Yeast CEN/ARS vector (Ura3) that contains sgRNA controlled by SNR 52 promoter, targeting endogenous TRE elements of pTET07 promoter AH057 Add to Cart
51023pSLQ1658-dCas9-EGFPdCas9 fuse to EGFP (Homo sapiens)Mammalian Expression, Retroviral, CRISPRDynamic Imaging of Genomic Loci in Living Human Cells by an Optimized CRISPR/Cas System. Cell. 2013 Dec 19;155(7):1479-91. doi: 10.1016/j.cell.2013.12.001. Template for NLS-dCas9-NLS-EGFP fusion protein for CRISPR imaging (the recipient vector can be TetON 3G promoter system) pMSCVpuro Add to Cart
51024pSLQ1651-sgTelomere(F+E)Optimized sgRNA (Homo sapiens)Mammalian Expression, Lentiviral, CRISPRDynamic Imaging of Genomic Loci in Living Human Cells by an Optimized CRISPR/Cas System. Cell. 2013 Dec 19;155(7):1479-91. doi: 10.1016/j.cell.2013.12.001. Lentiviral vector that contains an optimized S. pyogenes sgRNA targeting human telomeres pSico Add to Cart
51025pSLQ1661-sgMUC4-E3(F+E)optimized sgRNA (Homo sapiens)Mammalian Expression, Lentiviral, CRISPRDynamic Imaging of Genomic Loci in Living Human Cells by an Optimized CRISPR/Cas System. Cell. 2013 Dec 19;155(7):1479-91. doi: 10.1016/j.cell.2013.12.001. Lentiviral vector that contains an optimized S. pyogenes sgRNA targeting the repetitive sequence of human MUC4 exon 3 pSico MUC4 ASGP, HSA276359, MUC-4 Add to Cart
62277pJZC518dCas9 (Other)Yeast ExpressionEngineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds. Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 10.1016/j.cell.2014.11.052. Yeast dCas9 expression plasmid pNH605 Add to Cart
62278pJZC519dCas9-VP64 (Other)Yeast ExpressionEngineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds. Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 10.1016/j.cell.2014.11.052. Yeast dCas9-VP64 expression plasmid pNH605 Add to Cart
62279pJZC522MCP-VP64Yeast ExpressionEngineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds. Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 10.1016/j.cell.2014.11.052. MCP-VP64 fusion pNH603 Add to Cart
62280pJZC504PCP-VP64Yeast ExpressionEngineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds. Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 10.1016/j.cell.2014.11.052. PCP-VP64 fusion pNH603 Add to Cart
62281pJZC506COM-VP64Yeast ExpressionEngineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds. Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 10.1016/j.cell.2014.11.052. COM-VP64 fusion pNH603 Add to Cart
62282pJZC620MCP-VP64, PCP-VP64, dCas9Yeast ExpressionEngineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds. Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 10.1016/j.cell.2014.11.052. Expresses dCas9, MCP-VP64, and PCP-VP64 in Yeast cells pNH605 Add to Cart
62283pJZC638MCP-VP64, dCas9 (Other)Yeast ExpressionEngineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds. Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 10.1016/j.cell.2014.11.052. Expresses MCP-VP64 and dCas9 in Yeast cells pNH605 Add to Cart
62313pJZC545sgRNA + 1x MS2 binding moduleYeast ExpressionEngineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds. Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 10.1016/j.cell.2014.11.052. sgRNA with 1x MS2 for yeast cells pRS416 Add to Cart
62314pJZC583sgRNA + 2x MS2 binding moduleYeast ExpressionEngineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds. Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 10.1016/j.cell.2014.11.052. sgRNA with 2x MS2 for yeast cells pRS416 Add to Cart
62315pJZC588sgRNA + 2x MS2 (wt+f6) binding moduleYeast ExpressionEngineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds. Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 10.1016/j.cell.2014.11.052. sgRNA with 2x MS2 (wt+f6) for yeast cells pRS416 Add to Cart
62316pJZC548sgRNA + 1x PP7Yeast ExpressionEngineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds. Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 10.1016/j.cell.2014.11.052. sgRNA with 1x PP7 for yeast cells pRS416 Add to Cart
62317pJZC603sgRNA + 2x PP7 RNA binding moduleYeast ExpressionEngineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds. Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 10.1016/j.cell.2014.11.052. sgRNA with 2x PP7 for yeast cells pRS416 Add to Cart
62318pJZC572sgRNA + 1x com RNA binding moduleYeast ExpressionEngineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds. Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 10.1016/j.cell.2014.11.052. sgRNA with 1x com for yeast cells pRS416 Add to Cart
62319pJZC593sgRNA + MS2-PP7 RNA binding moduleYeast ExpressionEngineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds. Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 10.1016/j.cell.2014.11.052. sgRNA with MS2-PP7 for yeast cells pRS416 Add to Cart
62320pJZC523sgRNAYeast ExpressionEngineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds. Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 10.1016/j.cell.2014.11.052. sgRNA for yeast cells pRS416 Add to Cart
62321pJZC625sgRNA +1x MS2, Pol II promoter with ribozyme cleavageYeast ExpressionEngineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds. Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 10.1016/j.cell.2014.11.052. sgRNA with 1x MS2, Pol II promoter with ribozyme-gRNA-ribozyme design for yeast cells pSV616 Add to Cart
62322pJZC595MCP-VP64, dCas9Yeast ExpressionEngineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds. Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 10.1016/j.cell.2014.11.052. Expresses MCP-VP64 and dCas9 in Yeast cells pNH605 Add to Cart
62325pJZC35sgRNAMammalian Expression, LentiviralEngineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds. Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 10.1016/j.cell.2014.11.052. sgRNA for mammalian cells with mCherry marker MP177_U6 (derived from pSico) Add to Cart
62327pJZC32sgRNA, MCP-VP64Mammalian Expression, LentiviralEngineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds. Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 10.1016/j.cell.2014.11.052. sgRNA (no RNA aptamer addition) with MCP-VP64 effector for mammalian cells MP177_U6 (derived from pSico) Add to Cart
62328pJZC25sgRNA + 1x MS2 binding module, MCP-VP64Mammalian Expression, LentiviralEngineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds. Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 10.1016/j.cell.2014.11.052. sgRNA + 1x MS2 with MCP-VP64 effector for mammalian cells MP177_U6 (derived from pSico) Add to Cart
62330pJZC33sgRNA + 2x MS2 binding module, MCP-VP64Mammalian Expression, LentiviralEngineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds. Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 10.1016/j.cell.2014.11.052. sgRNA + 2x MS2 with MCP-VP64 effector for mammalian cells MP177_U6 (derived from pSico) Add to Cart
62331pJZC34sgRNA + 2x MS2(wt+f6) binding module, MCP-VP64Mammalian Expression, LentiviralEngineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds. Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 10.1016/j.cell.2014.11.052. sgRNA + 2x MS2(wt+f6) with MCP-VP64 effector for mammalian cells MP177_U6 (derived from pSico) Add to Cart
62332pJZC41sgRNA, PCP-VP64Mammalian Expression, LentiviralEngineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds. Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 10.1016/j.cell.2014.11.052. sgRNA (no RNA aptamer addition) with PCP-VP64 effector for mammalian cells MP177_U6 (derived from pSico) Add to Cart
62333pJZC39sgRNA + 1x PP7, mCherryMammalian Expression, LentiviralEngineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds. Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 10.1016/j.cell.2014.11.052. sgRNA + 1x PP7 with mCherry for mammalian cells MP177_U6 (derived from pSico) Add to Cart
62334pJZC40sgRNA + 2x PP7, mCherryMammalian Expression, LentiviralEngineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds. Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 10.1016/j.cell.2014.11.052. sgRNA + 2x PP7 with mCherry for mammalian cells MP177_U6 (derived from pSico) Add to Cart
62335pJZC101sgRNA, COM-VP64Mammalian Expression, LentiviralEngineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds. Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 10.1016/j.cell.2014.11.052. sgRNA (no RNA aptamer addition) with COM-VP64 effector for mammalian cells MP177_U6 (derived from pSico) Add to Cart
62336pJZC48sgRNA + 1x COM binding module, COM-VP64Mammalian Expression, LentiviralEngineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds. Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 10.1016/j.cell.2014.11.052. sgRNA + 1x COM with COM-VP64 effector for mammalian cells MP177_U6 (derived from pSico) Add to Cart
62337pJZC102sgRNAMammalian Expression, LentiviralEngineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds. Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 10.1016/j.cell.2014.11.052. sgRNA for mammalian cells with mCherry marker MP177_U6 (derived from pSico) Add to Cart
62338pJZC77sgRNA, COM-KRABMammalian Expression, LentiviralEngineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds. Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 10.1016/j.cell.2014.11.052. sgRNA (no RNA aptamer addition) with COM-KRAB effector for mammalian cells MP177_U6 (derived from pSico) Add to Cart
62339pJZC78sgRNA + 1x COM binding module, COM-KRABMammalian Expression, LentiviralEngineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds. Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 10.1016/j.cell.2014.11.052. sgRNA + 1x COM with COM-KRAB effector for mammalian cells MP177_U6 (derived from pSico) Add to Cart
62340pJZC103sgRNAMammalian Expression, LentiviralEngineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds. Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 10.1016/j.cell.2014.11.052. sgRNA for mammalian cells with mCherry marker MP177_U6 (derived from pSico) Add to Cart
62341pJZC73sgRNA, COM-KRABMammalian Expression, LentiviralEngineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds. Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 10.1016/j.cell.2014.11.052. sgRNA (no RNA aptamer addition) with COM-KRAB effector for mammalian cells MP177_U6 (derived from pSico) Add to Cart
62342pJZC74sgRNA + 1x COM binding module, COM-KRABMammalian Expression, LentiviralEngineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds. Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 10.1016/j.cell.2014.11.052. sgRNA + 1x COM with COM-KRAB effector for mammalian cells MP177_U6 (derived from pSico) Add to Cart
62344pJZC116sgRNA + 2x MS2 (wt+f6) binding module, MCP-VP64Mammalian Expression, LentiviralEngineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds. Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 10.1016/j.cell.2014.11.052. sgRNA + 2x MS2(wt+f6) with MCP-VP64 effector for mammalian cells, marked by BFP MP177_U6 (derived from pSico) Add to Cart
63709pP2A_NLS_sfGFPNanog homologous arm, p2A, NLS, sfGFP, Nanog homologous arm (Mus musculus)Just a donor DNA with Nanog homologous arm for knock-in sfGFPSmall Molecules Enhance CRISPR Genome Editing in Pluripotent Stem Cells. Cell Stem Cell. 2015 Feb 5;16(2):142-7. doi: 10.1016/j.stem.2015.01.003. Expressing P2A_NLS_sfGFP with homologous arm of Nanog. pUC19 Add to Cart
63710pSLQ1371_sgNanogsgNanog, CMV promoter, puro, mCherry (Mus musculus)Mammalian ExpressionSmall Molecules Enhance CRISPR Genome Editing in Pluripotent Stem Cells. Cell Stem Cell. 2015 Feb 5;16(2):142-7. doi: 10.1016/j.stem.2015.01.003. Express sgNanog in mammalian cells of mice. pHR Add to Cart
63711pSLQ1653_sgSOD1sgSOD1, CAG promoter, mCherry, puroMammalian ExpressionSmall Molecules Enhance CRISPR Genome Editing in Pluripotent Stem Cells. Cell Stem Cell. 2015 Feb 5;16(2):142-7. doi: 10.1016/j.stem.2015.01.003. Express sgSOD1 in mammalian cells of human. pHR Add to Cart
63712pX330_sgACTA2ACTA2 (Homo sapiens)Mammalian ExpressionSmall Molecules Enhance CRISPR Genome Editing in Pluripotent Stem Cells. Cell Stem Cell. 2015 Feb 5;16(2):142-7. doi: 10.1016/j.stem.2015.01.003. Express sgACTA2 in mammalian cells. pX330 Add to Cart
66564pJZC42sgRNA + 1XPP7, PCP-VP64 IRES mCherryMammalian ExpressionEngineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds. Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 10.1016/j.cell.2014.11.052. sgRNA + 1XPP7 with PCP-VP64 effector for mammalian cells, marked by mCherry pSico Add to Cart
66565pJZC43sgRNA + 2XPP7, PCP-VP64 IRES mCherryMammalian ExpressionEngineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds. Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 10.1016/j.cell.2014.11.052. sgRNA + 2XPP7 with PCP-VP64 effector for mammalian cells, marked by mCherry pSico Add to Cart
79873pJMP1dCas9 (Other)Bacterial Expression, CRISPRA Comprehensive, CRISPR-based Functional Analysis of Essential Genes in Bacteria. Cell. 2016 Jun 2;165(6):1493-506. doi: 10.1016/j.cell.2016.05.003. Epub 2016 May 26. Bacillus subtilis dCas9 expression vector; integrates into lacA/ganA pAX01 Add to Cart
79874pJMP2sgRNA RR1 (Other)Bacterial Expression, CRISPRA Comprehensive, CRISPR-based Functional Analysis of Essential Genes in Bacteria. Cell. 2016 Jun 2;165(6):1493-506. doi: 10.1016/j.cell.2016.05.003. Epub 2016 May 26. Bacillus subtilis sgRNA expression vector; integrates into amyE pDG1662 Add to Cart
79875pJMP3sgRNA RR1 (Other)Bacterial Expression, CRISPRA Comprehensive, CRISPR-based Functional Analysis of Essential Genes in Bacteria. Cell. 2016 Jun 2;165(6):1493-506. doi: 10.1016/j.cell.2016.05.003. Epub 2016 May 26. Bacillus subtilis sgRNA expression vector; integrates into thrC pDG1731 Add to Cart
84239pSLQ2817 pPB: CAG-PYL1-VPR-IRES-Puro-WPRE-SV40PA PGK-ABI-tagBFP-SpdCas9ABI-tagBFP-Sp dCas9 (Arabidopsis thaliana), PYL1-VPR (Arabidopsis thaliana)Mammalian Expression, CRISPR, Synthetic Biology ; PiggyBacComplex transcriptional modulation with orthogonal and inducible dCas9 regulators. Nat Methods. 2016 Oct 24. doi: 10.1038/nmeth.4042. Expresses ABA-inducible VPR-Sp dCas9 PB Add to Cart
84240pSLQ2812 pPB: CAG-GID1-VPR-IRES-Puro-WPRE PGK-GAI-tagBFP-SpdCas9GAI-tagBFP-Sp dCas9 (Arabidopsis thaliana), GID1-VPR (Arabidopsis thaliana)Mammalian Expression, CRISPR, Synthetic Biology ; PiggyBacComplex transcriptional modulation with orthogonal and inducible dCas9 regulators. Nat Methods. 2016 Oct 24. doi: 10.1038/nmeth.4042. Expresses GA-inducible VPR-Sp dCas9 PB Add to Cart
84241pSLQ2818 pPB: CAG-PYL1-KRAB-IRES-Puro-WPRE-SV40PA PGK-ABI-tagBFP-SpdCas9ABI-tagBFP-Sp dCas9 (Arabidopsis thaliana), PYL1-KRAB (Arabidopsis thaliana)Mammalian Expression, CRISPR, Synthetic Biology ; PiggyBacComplex transcriptional modulation with orthogonal and inducible dCas9 regulators. Nat Methods. 2016 Oct 24. doi: 10.1038/nmeth.4042. Expresses ABA-inducible KRAB-Sp dCas9 PB Add to Cart
84242pSLQ2813 pPB: CAG-GID1-KRAB-IRES-Puro-WPRE PGK-ABI-tagBFP-SpdCas9GAI-tagBFP-Sp dCas9 (Arabidopsis thaliana), GID1-KRAB (Arabidopsis thaliana)Mammalian Expression, CRISPR, Synthetic Biology ; PiggyBacComplex transcriptional modulation with orthogonal and inducible dCas9 regulators. Nat Methods. 2016 Oct 24. doi: 10.1038/nmeth.4042. Expresses GA-inducible KRAB-Sp dCas9 PB Add to Cart
84243pSLQ2833 pPB: CAG-PYL1-VPR-IRES-Zeocin-WPRE-SV40PA PGK-ABI-tagBFP-SadCas9ABI-tagBFP-Sa dCas9 (Arabidopsis thaliana), PYL1-VPR (Arabidopsis thaliana)Mammalian Expression, CRISPR, Synthetic Biology ; PiggyBacComplex transcriptional modulation with orthogonal and inducible dCas9 regulators. Nat Methods. 2016 Oct 24. doi: 10.1038/nmeth.4042. Expresses ABA-inducible VPR-Sa dCas9 PB Add to Cart
84244pSLQ2842 pPB: CAG-GID1-VPR-IRES-Zeocin-WPRE PGK-GAI-tagBFP-SadCas9GAI-tagBFP-Sa dCas9 (Arabidopsis thaliana), GID1-VPR (Arabidopsis thaliana)Mammalian Expression, CRISPR, Synthetic Biology ; PiggyBacComplex transcriptional modulation with orthogonal and inducible dCas9 regulators. Nat Methods. 2016 Oct 24. doi: 10.1038/nmeth.4042. Expresses GA-inducible VPR-Sa dCas9 PB Add to Cart
84245pSLQ2815 pPB: CAG-Puro-WPRE PGK-KRAB-tagBFP-SpdCas9KRAB-tagBFP-Sp dCas9 (Synthetic), PuroMammalian Expression, CRISPR, Synthetic Biology ; PiggyBacComplex transcriptional modulation with orthogonal and inducible dCas9 regulators. Nat Methods. 2016 Oct 24. doi: 10.1038/nmeth.4042. Expresses direct fusion KRAB-Sp dCas9 PB Add to Cart
84246pSLQ2840 pPB: CAG-Puro-WPRE PGK-VPR-tagBFP-SadCas9VPR-tagBFP-Sa dCas9 (Synthetic), PuroMammalian Expression, CRISPR, Synthetic Biology ; PiggyBacComplex transcriptional modulation with orthogonal and inducible dCas9 regulators. Nat Methods. 2016 Oct 24. doi: 10.1038/nmeth.4042. Expresses direct fusion VPR-Sa dCas9 PB Add to Cart
84247pSLQ2814 pPB: CAG-Puro-WPRE PGK-VPR-tagBFP-SpdCas9VPR-tagBFP-Sp dCas9 (Synthetic), PuroMammalian Expression, CRISPR, Synthetic Biology ; PiggyBacComplex transcriptional modulation with orthogonal and inducible dCas9 regulators. Nat Methods. 2016 Oct 24. doi: 10.1038/nmeth.4042. Expresses direct fusion VPR-Sp dCas9 PB Add to Cart
84248pSLQ1870-1 pHR: U6-SpsgTRE3G CMV-mCherrySp sgTRE3G (Synthetic)Mammalian Expression, Lentiviral, CRISPRComplex transcriptional modulation with orthogonal and inducible dCas9 regulators. Nat Methods. 2016 Oct 24. doi: 10.1038/nmeth.4042. Expresses Sp sgTRE3G gRNA with a mCherry fluorescent marker pHR Add to Cart
84249pSLQ1869-1 pHR: U6-SpsgSV40 CMV-mCherrySp sgSV40 (Synthetic)Mammalian Expression, Lentiviral, CRISPRComplex transcriptional modulation with orthogonal and inducible dCas9 regulators. Nat Methods. 2016 Oct 24. doi: 10.1038/nmeth.4042. Expresses Sp sgSV40 gRNA with a mCherry fluorescent marker pHR Add to Cart
84250pSLQ2806-2 pHR: U6-Sasgv2TRE3G CMV-mCherrySa sgTRE3G (Synthetic)Mammalian Expression, Lentiviral, CRISPRComplex transcriptional modulation with orthogonal and inducible dCas9 regulators. Nat Methods. 2016 Oct 24. doi: 10.1038/nmeth.4042. Expresses optimized Sa sgTRE3G gRNA with a mCherry fluorescent marker pHR Add to Cart
84251pSLQ1852-2 pHR: U6-SpsgCD95-1 CMV-EGFPSp sgCD95-1 (Homo sapiens)Mammalian Expression, Lentiviral, CRISPRComplex transcriptional modulation with orthogonal and inducible dCas9 regulators. Nat Methods. 2016 Oct 24. doi: 10.1038/nmeth.4042. Expresses Sp sgCD95-1 gRNA with an EGFP fluorescent marker pHR Add to Cart
84252pSLQ1852-3 pHR: U6-SpsgCD95-2 CMV-EGFPSp sgCD95-2 (Homo sapiens)Mammalian Expression, Lentiviral, CRISPRComplex transcriptional modulation with orthogonal and inducible dCas9 regulators. Nat Methods. 2016 Oct 24. doi: 10.1038/nmeth.4042. Expresses Sp sgCD95-2 gRNA with an EGFP fluorescent marker pHR Add to Cart
84253pSLQ1852-5 pHR: U6-SpsgCD95-3 CMV-EGFPSp sgCD95-3 (Homo sapiens)Mammalian Expression, Lentiviral, CRISPRComplex transcriptional modulation with orthogonal and inducible dCas9 regulators. Nat Methods. 2016 Oct 24. doi: 10.1038/nmeth.4042. Expresses Sp sgCD95-3 gRNA with an EGFP fluorescent marker pHR Add to Cart
84254pSLQ2853-3 pHR: U6-Sasgv2CXCR4-1 CMV-EGFPSa sgCXCR4-1 (Homo sapiens)Mammalian Expression, Lentiviral, CRISPRComplex transcriptional modulation with orthogonal and inducible dCas9 regulators. Nat Methods. 2016 Oct 24. doi: 10.1038/nmeth.4042. Expresses optimized Sa sgCXCR4-1 gRNA with an EGFP fluorescent marker pHR Add to Cart
84255pSLQ2853-4 pHR: U6-Sasgv2CXCR4-2 CMV-EGFPSa sgCXCR4-2 (Homo sapiens)Mammalian Expression, Lentiviral, CRISPRComplex transcriptional modulation with orthogonal and inducible dCas9 regulators. Nat Methods. 2016 Oct 24. doi: 10.1038/nmeth.4042. Expresses optimized Sa sgCXCR4-2 gRNA with an EGFP fluorescent marker pHR Add to Cart
84256pSLQ2853-5 pHR: U6-Sasgv2CXCR4-3 CMV-EGFPSa sgCXCR4-3 (Homo sapiens)Mammalian Expression, Lentiviral, CRISPRComplex transcriptional modulation with orthogonal and inducible dCas9 regulators. Nat Methods. 2016 Oct 24. doi: 10.1038/nmeth.4042. Expresses optimized Sa sgCXCR4-3 gRNA with an EGFP fluorescent marker pHR Add to Cart
84258pSLQ2804 pHR: U6-SpsgTRE3G CMV-PYL1-VPR-IRES-mCherrySp sgTRE3G (Synthetic), PYL1-VPR (Arabidopsis thaliana)Mammalian Expression, Lentiviral, CRISPRComplex transcriptional modulation with orthogonal and inducible dCas9 regulators. Nat Methods. 2016 Oct 24. doi: 10.1038/nmeth.4042. Expresses Sp sgTRE3G gRNA with ABA-inducible VPR and mCherry for OR gate pHR Add to Cart
84260pSLQ2827 pHR: U6-SpsgSV40 CMV-PYL1-KRAB-IRES-mCherrySp sgSV40 (Synthetic), PYL1-KRAB (Arabidopsis thaliana)Mammalian Expression, Lentiviral, CRISPRComplex transcriptional modulation with orthogonal and inducible dCas9 regulators. Nat Methods. 2016 Oct 24. doi: 10.1038/nmeth.4042. Expresses Sp sgSV40 gRNA with ABA-inducible KRAB and mCherry for diametric regulation pHR Add to Cart
84261pSLQ2816 pPB: CAG-PYL1-VPR-p2A-GID1-ABI-WPRE PGK-GAI-tagBFP-SpdCas9GAI-tagBFP-Sp dCas9 (Arabidopsis thaliana), PYL1-VPR-p2a-GID1-ABI (Arabidopsis thaliana)Mammalian Expression, CRISPR, Synthetic Biology ; PiggyBacComplex transcriptional modulation with orthogonal and inducible dCas9 regulators. Nat Methods. 2016 Oct 24. doi: 10.1038/nmeth.4042. Expresses AND gate ABA/GA-inducible VPR-Sp dCas9 PB Add to Cart