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Stephen Michnick Lab Plasmids


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Title Authors Publication
Michnick Lab IFP PCA plasmids (unpublished)
ID Plasmid Gene/Insert Vector Type Publication Hidden Extra Search Info  
52893p413GAL1-Zipp.-L-IFP-F[2] (Yeast)Zipp.-L-IFP-F[2] (Other)Yeast Expression Michnick Lab IFP PCA plasmids (unpublished) Plasmid+insert encoding GCN4-leucine zipper fused to IFP-F[2] by the linker (GGGGS) X 2. Zipper-L-IFP-F[2] (XbaI/XhoI). Zipper is (XbaI /ClaI). Plasmid confers ampicillin resistance to DH5α. p413GAL1 Add to Cart
52895p416GAL1-Zipp.-L-IFP-F[1] (Yeast)Zipp.-L-IFP-F[1] (Other)Yeast Expression Michnick Lab IFP PCA plasmids (unpublished) Plasmid+insert encoding GCN4-leucine zipper fused to IFP-F[1] by the linker (GGGGS) X 2. Zipper-L-IFP-F[1] (XbaI/XhoI). Zipper is (XbaI /ClaI). Plasmid confers ampicillin resistance to DH5α. p416GAL1 Add to Cart
52896p416GAL1-Skn7t-L-IFP-F[1] (Yeast)Skn7t-L-IFP-F[1] (Saccharomyces cerevisiae)Yeast Expression Michnick Lab IFP PCA plasmids (unpublished) Skn7t replaces the zipper and is fused to the IFP-F[1] through the linker (GGGGS) X 2. Plasmid confers ampicillin resistance to DH5α. p416GAL1 Add to Cart
52897p416GAL1-Ssk1t-L-IFP-F[1] (Yeast)Ssk1t-L-IFP-F[1] (Saccharomyces cerevisiae)Yeast Expression Michnick Lab IFP PCA plasmids (unpublished) Ssk1t replaces the zipper and is fused to the IFP-F[1] through the linker (GGGGS) X 2. Plasmid confers ampicillin resistance to DH5α. p416GAL1 Add to Cart
52898p413GAL1-Ypd*1-L-IFP-F[2] (Yeast)Ypd*1-L-IFP-F[2] (Saccharomyces cerevisiae)Yeast Expression Michnick Lab IFP PCA plasmids (unpublished) YpdI* is fused to the IFP-F[2] by (GGGGS) X 2. Ypd1*/Skn7t: no signal, Ypd1*/Ssk1t: weak signal. Internal ClaI site in Ypd*I. Plasmid confers ampicillin resistance to DH5α. p413GAL1 Add to Cart
52899pAG25-L-IFP-F[1] (clon 3)L-IFP-F[1] (Other)Vector for homologous recombination Michnick Lab IFP PCA plasmids (unpublished) Plasmid template for PCR of cassettes for HR, used to introduce IFP F[1] fragments 3’ to the ORF of the genes to study. Plasmid confers ampicillin resistance to DH5α. pAG25 Add to Cart
52900pAG32-L-IFP-F[2] (clon 3)L-IFP-F[2] (Other)Vector for homologous recombination Michnick Lab IFP PCA plasmids (unpublished) Plasmid template for PCR of cassettes for HR, used to introduce IFP F[2] fragments 3’ to the ORF of the genes to study. Plasmid confers ampicillin resistance to DH5α. pAG32 Add to Cart
52901pLpC-L-IFP-F[1] (mammalian)L-IFP-F[1] (Other)Mammalian Expression, Retroviral Michnick Lab IFP PCA plasmids (unpublished) Binary retroviral pl. (puromycin). MCS:(GGGGS)X 2 + IFP-F[1] (ClaI/EcoRI). Insert can be subcloned betweeen the MCS. Plasmid confers ampicillin resistance to DH5α. pLpC Add to Cart
52902pLpC-L-IFP-F[2] (mammalian)L-IFP-F[2] (Other)Mammalian Expression, Retroviral Michnick Lab IFP PCA plasmids (unpublished) Binary retroviral pl. (puromycin). MCS:(GGGGS)X 2 + IFP-F[2] (ClaI/EcoRI). Insert can be subcloned betweeen the MCS. Plasmid confers ampicillin resistance to DH5α. pLpC Add to Cart
52903pLpC-Reg-L-IFP-F[2]+Venus (mammalian)Reg-L-IFP-F[2]+Venus (Homo sapiens)Mammalian Expression, Retroviral Michnick Lab IFP PCA plasmids (unpublished) Binary retroviral pl. (puromycin). Reg:Linker (GGGGS)X2+IFP-F[2] (PacI/EcoRI). In the second MCS, the Venus fluorescent protein is cloned in between PmeI/SalI.Confers ampicillin resistance to DH5α. pLpC Add to Cart
52905pLpC-Cat-L-IFP-F[1]+Plum (mammalian)Cat-L-IFP-F[1]+Plum (Homo sapiens)Mammalian Expression, Retroviral Michnick Lab IFP PCA plasmids (unpublished) Binary retroviral pl. (puromycin). Cat(PacI/ClaI):Linker (GGGGS)X2+IFP-F[2]. In the second MCS, the Plum fluorescent protein is cloned in between PmeI/SalI.Confers ampicillin resistance to DH5α. pLpC Add to Cart
52906pLpC-Zipp.-L-IFP-F[2]+Venus (mammalian)Zipp.-L-IFP-F[2]+Venus (Other)Mammalian Expression, Retroviral Michnick Lab IFP PCA plasmids (unpublished) Binary retroviral pl. (puromycin). Zipper (PacI/ClaI):Linker (GGGGS)X2+IFP-F[2]. In the second MCS, the Venus fluorescent protein is cloned in between PmeI/SalI.Confers ampicillin resistance to DH5α. pLpC Add to Cart
52907pLpC-Zipp.-L-IFP-F[1]+Plum (mammalian)Zipp.-L-IFP-F[1]+Plum (Other)Mammalian Expression, Retroviral Michnick Lab IFP PCA plasmids (unpublished) Binary retroviral pl. (puromycin). Zipper (PacI/ClaI):Linker (GGGGS)X2+IFP-F[1]. In the second MCS, the Plum fluorescent protein is cloned in between PmeI/SalI. Confers ampicillin resistance to DH5α. pLpC Add to Cart