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Stephen Royle Lab Plasmids


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Title Authors Publication
A TACC3/ch-TOG/clathrin complex stabilises kinetochore fibres by inter-microtubule bridging. Booth DG, Hood FE, Prior IA, Royle SJ EMBO J. 2011 Mar 2;30(5):906-19. doi: 10.1038/emboj.2011.15. Epub 2011 Feb 4.
Specific removal of TACC3-ch-TOG-clathrin at metaphase deregulates kinetochore fiber tension. Cheeseman LP, Harry EF, McAinsh AD, Prior IA, Royle SJ J Cell Sci. 2013 May 1;126(Pt 9):2102-13. doi: 10.1242/jcs.124834. Epub 2013 Mar 26.
The mesh is a network of microtubule connectors that stabilizes individual kinetochore fibers of the mitotic spindle. Nixon FM, Gutierrez-Caballero C, Hood FE, Booth DG, Prior IA, Royle SJ Elife. 2015 Jun 19;4. doi: 10.7554/eLife.07635.
TACC3-ch-TOG track the growing tips of microtubules independently of clathrin and Aurora-A phosphorylation. Gutierrez-Caballero C, Burgess SG, Bayliss R, Royle SJ Biol Open. 2015 Jan 16;4(2):170-9. doi: 10.1242/bio.201410843.
ID Plasmid Gene/Insert Vector Type Publication Hidden Extra Search Info  
59352pMito-mCherry-FRBMammalian ExpressionSpecific removal of TACC3-ch-TOG-clathrin at metaphase deregulates kinetochore fiber tension. J Cell Sci. 2013 May 1;126(Pt 9):2102-13. doi: 10.1242/jcs.124834. Epub 2013 Mar 26. Expresses mCherry-tagged MitoTrap in mammalian cells. pMito
  • Tags / Fusion Proteins
    • mCherry (C terminal on backbone)
    • FRB (C terminal on backbone)
  • Add to Cart
    59353GFP-FKBP-LCaClathrin Light Chain A (Homo sapiens)Mammalian ExpressionSpecific removal of TACC3-ch-TOG-clathrin at metaphase deregulates kinetochore fiber tension. J Cell Sci. 2013 May 1;126(Pt 9):2102-13. doi: 10.1242/jcs.124834. Epub 2013 Mar 26. Expresses human clathrin light chain A tagged with GFP & FKBP in mammalian cells. pEGFP-C1 Add to Cart
    59354pBrain-GFP-FKBP-TACC3-shTACC3Transforming Acidic Coiled Coil protein 3 (Homo sapiens)Mammalian Expression, RNAiSpecific removal of TACC3-ch-TOG-clathrin at metaphase deregulates kinetochore fiber tension. J Cell Sci. 2013 May 1;126(Pt 9):2102-13. doi: 10.1242/jcs.124834. Epub 2013 Mar 26. Knocks down endogenous TACC3 and expresses RNAi-resistant human TACC3 tagged with GFP & FKBP in mammalian cells. pBrain TACC3 ERIC-1, ERIC1 Add to Cart
    59355pBrain-GFP-shTACC3EGFP (Homo sapiens)Mammalian Expression, RNAiA TACC3/ch-TOG/clathrin complex stabilises kinetochore fibres by inter-microtubule bridging. EMBO J. 2011 Mar 2;30(5):906-19. doi: 10.1038/emboj.2011.15. Epub 2011 Feb 4. Knocks down TACC3 in mammalian cells via shRNA and co-expresses GFP. pBrain Add to Cart
    59356pBrain-GFP-TACC3KDP-shTACC3Transforming Acidic Coiled Coil protein 3 (Homo sapiens)Mammalian Expression, RNAiA TACC3/ch-TOG/clathrin complex stabilises kinetochore fibres by inter-microtubule bridging. EMBO J. 2011 Mar 2;30(5):906-19. doi: 10.1038/emboj.2011.15. Epub 2011 Feb 4. Knocks down endogenous TACC3 and re-expresses knockdown-proof (RNAi-resistant) GFP-tagged human TACC3. pBrain TACC3 ERIC-1, ERIC1 Add to Cart
    59357pBrain-GFP-TACC3KDP(S558A)-shTACC3Transforming Acidic Coiled Coil protein 3 (Homo sapiens)Mammalian Expression, RNAiA TACC3/ch-TOG/clathrin complex stabilises kinetochore fibres by inter-microtubule bridging. EMBO J. 2011 Mar 2;30(5):906-19. doi: 10.1038/emboj.2011.15. Epub 2011 Feb 4. Expresses GFP-tagged human TACC3 (RNAi-resistant) with S558A substitution and knocks down endogenous TACC3 via shRNA. pBrain TACC3 ERIC-1, ERIC1 Add to Cart
    59799GFP-CHC17KDPCHC17 (Homo sapiens)Mammalian ExpressionA TACC3/ch-TOG/clathrin complex stabilises kinetochore fibres by inter-microtubule bridging. EMBO J. 2011 Mar 2;30(5):906-19. doi: 10.1038/emboj.2011.15. Epub 2011 Feb 4. Expresses knockdown-proof human CHC17 tagged with GFP. pEGFP-C1 CLTC CHC, CHC17, CLH-17, CLTCL2, Hc Add to Cart
    60004pBrain-GFP-shGL2EGFP (Other)Mammalian Expression, RNAiA TACC3/ch-TOG/clathrin complex stabilises kinetochore fibres by inter-microtubule bridging. EMBO J. 2011 Mar 2;30(5):906-19. doi: 10.1038/emboj.2011.15. Epub 2011 Feb 4. Co-expresses control GL2 shRNA and GFP. pBrain Add to Cart
    60005pMito-PAGFP-FRB.Mammalian Expression ; KnocksidewaysSpecific removal of TACC3-ch-TOG-clathrin at metaphase deregulates kinetochore fiber tension. J Cell Sci. 2013 May 1;126(Pt 9):2102-13. doi: 10.1242/jcs.124834. Epub 2013 Mar 26. Expresses MitoTrap tagged with photo-activatable GFP in mammalian cells. pMito
  • Tags / Fusion Proteins
    • PAGFP (C terminal on backbone)
    • FRB (C terminal on backbone)
  • Add to Cart
    69106GST-TACC3-His6Transforming Acidic Coiled Coil protein 3 (Homo sapiens)Bacterial ExpressionThe mesh is a network of microtubule connectors that stabilizes individual kinetochore fibers of the mitotic spindle. Elife. 2015 Jun 19;4. doi: 10.7554/eLife.07635. For bacterial expression of recombinant human TACC3 tagged with GST and His. pGEX-6p-1 TACC3 ERIC-1, ERIC1 Add to Cart
    69107GST-TPX2(1-43)Targeting Protein for Xklp2 (Homo sapiens)Bacterial ExpressionThe mesh is a network of microtubule connectors that stabilizes individual kinetochore fibers of the mitotic spindle. Elife. 2015 Jun 19;4. doi: 10.7554/eLife.07635. For bacterial expression of human TPX2(1-43aa) tagged with GST. pGEX-6p-1 TPX2 C20orf1, C20orf2, DIL-2, DIL2, FLS353, GD:C20orf1, HCA519, HCTP4, REPP86, p100 Add to Cart
    69108MBP-ch-TOG-His6Colonic Hepatic Tumour Over-expressed Gene (Homo sapiens)Bacterial ExpressionThe mesh is a network of microtubule connectors that stabilizes individual kinetochore fibers of the mitotic spindle. Elife. 2015 Jun 19;4. doi: 10.7554/eLife.07635. For bacterial expression of recombinant human ch-TOG tagged with MBP and His. pMALPre-His CKAP5 CHTOG, MSPS, TOG, TOGp, ch-TOG Add to Cart
    69109MBP-GTSE1-His6G2- and S-phase Expressed 1 (Homo sapiens)Bacterial ExpressionThe mesh is a network of microtubule connectors that stabilizes individual kinetochore fibers of the mitotic spindle. Elife. 2015 Jun 19;4. doi: 10.7554/eLife.07635. For bacterial expression of recombinant human GTSE1 tagged with MBP and His. pMALPre-His GTSE1 B99 Add to Cart
    69110MBP-GTSE1(long)-His6G2- and S-phase Expressed 1 (Homo sapiens)Bacterial ExpressionThe mesh is a network of microtubule connectors that stabilizes individual kinetochore fibers of the mitotic spindle. Elife. 2015 Jun 19;4. doi: 10.7554/eLife.07635. For bacterial expression of recombinant human GTSE1 (longer isoform) tagged with MBP and His. pMALPre-His GTSE1 B99 Add to Cart
    69111His6-PRC1Protein Regulator of Cytokinesis 1 (Homo sapiens)Bacterial ExpressionThe mesh is a network of microtubule connectors that stabilizes individual kinetochore fibers of the mitotic spindle. Elife. 2015 Jun 19;4. doi: 10.7554/eLife.07635. For bacterial expression of recombinant human PRC1 tagged with His. pRSET-B PRC1 ASE1 Add to Cart
    69112ch-TOGKDP-GFPColonic Hepatic Tumour Over-expressed Gene (Homo sapiens)Mammalian ExpressionTACC3-ch-TOG track the growing tips of microtubules independently of clathrin and Aurora-A phosphorylation. Biol Open. 2015 Jan 16;4(2):170-9. doi: 10.1242/bio.201410843. For mammalian expression of knockdown-proof human ch-TOG tagged with EGFP. pEGFP-N3 CKAP5 CHTOG, MSPS, TOG, TOGp, ch-TOG Add to Cart
    69113pBrain-ch-TOGKDP-GFP-shch-TOGColonic Hepatic Tumour Over-expressed Gene (Homo sapiens), shch-TOG (Homo sapiens)Mammalian Expression, RNAiTACC3-ch-TOG track the growing tips of microtubules independently of clathrin and Aurora-A phosphorylation. Biol Open. 2015 Jan 16;4(2):170-9. doi: 10.1242/bio.201410843. Dual-promoter plasmid to express knockdown-proof human ch-TOG tagged with EGFP along with shRNA to knock down endogenous ch-TOG in mammalian cells. pBrain Add to Cart
    69114pBrain-ch-TOGKDP(L1939A,L1942A)-GFP-shch-TOGColonic Hepatic Tumour Over-expressed Gene (Homo sapiens), shch-TOG (Homo sapiens)Mammalian Expression, RNAiTACC3-ch-TOG track the growing tips of microtubules independently of clathrin and Aurora-A phosphorylation. Biol Open. 2015 Jan 16;4(2):170-9. doi: 10.1242/bio.201410843. Dual-promoter plasmid to express knockdown-proof human ch-TOG with L1939A and L1942A substitutions tagged with EGFP along with shRNA to knock down endogenous ch-TOG in mammalian cells. pBrain Add to Cart
    69115pBrain-GFP-TACC3KDP∆678-681-shTACC3Transforming Acidic Coiled Coil protein 3 (Homo sapiens), shTACC3 (Homo sapiens)Mammalian Expression, RNAiTACC3-ch-TOG track the growing tips of microtubules independently of clathrin and Aurora-A phosphorylation. Biol Open. 2015 Jan 16;4(2):170-9. doi: 10.1242/bio.201410843. Dual-promoter plasmid to express knockdown-proof human TACC3 (amino acids 678-681 deleted) tagged with EGFP along with shRNA to knock down endogenous TACC3 in mammalian cells. pBrain TACC3 ERIC-1, ERIC1 Add to Cart
    69116pBrain-GFP-TACC3KDP∆682-688-shTACC3Transforming Acidic Coiled Coil protein 3 (Homo sapiens), shTACC3 (Homo sapiens)Mammalian Expression, RNAiTACC3-ch-TOG track the growing tips of microtubules independently of clathrin and Aurora-A phosphorylation. Biol Open. 2015 Jan 16;4(2):170-9. doi: 10.1242/bio.201410843. Dual-promoter plasmid to express knockdown-proof human TACC3 (682-688aa ch-TOG binding site deleted) tagged with EGFP along with shRNA to knock down endogenous TACC3 in mammalian cells. pBrain TACC3 ERIC-1, ERIC1 Add to Cart
    69117pBrain-GFP-TACC3KDP(L566,567A)-shTACC3Transforming Acidic Coiled Coil protein 3 (Homo sapiens), shTACC3 (Homo sapiens)Mammalian Expression, RNAiTACC3-ch-TOG track the growing tips of microtubules independently of clathrin and Aurora-A phosphorylation. Biol Open. 2015 Jan 16;4(2):170-9. doi: 10.1242/bio.201410843. Dual-promoter plasmid to express knockdown-proof human TACC3 (L566A and L567A substitutions) tagged with EGFP along with shRNA to knock down endogenous TACC3 in mammalian cells. pBrain TACC3 ERIC-1, ERIC1 Add to Cart