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Musunuru/Cowan Lab TALEN Kit
(Kit # 1000000034 )

Depositing Labs:   Kiran Musunuru, Chad Cowan

TALENs are fusions of transcription activator-like (TAL) effectors of plant pathogenic Xanthomonas spp. to the FokI nuclease. TALENs bind and cleave DNA in pairs, and their binding specificity is determined by customizable arrays of polymorphic amino acid repeats in the TAL effectors. This collection and accompanying documentation allow one to efficiently assemble TALEN constructs with custom repeat arrays, each containing 15 repeats.

This kit consists of 9 plates, and will be shipped as bacterial glycerol stocks, 96-well format, on dry ice. Samples should be frozen at -80 degrees Celsius immediately upon arrival. Individual plasmids can be ordered at $65 per plasmid, and will be shipped as bacterial stabs.

Add to Cart
$2800 USD + shipping
Available to Academic and Nonprofits Only

Original Publication

A TALEN Genome-Editing System for Generating Human Stem Cell-Based Disease Models. Ding Q, Lee Y, Schaefer E, Peters D, Veres A, Kim K, Kuperwasser N, Motola D, Meissner T, Hendriks W, Trevisan M, Gupta R, Moisan A, Banks E, Friesen M, Schinzel R, Xia F, Tang A, Xia Y, Figueroa E, Wann A, Ahfeldt T, Daheron L, Zhang F, Rubin L, Peng L, Chung R, Musunuru K, Cowan C. Cell Stem Cell. 2013 Feb 7;12(2):238-51. doi: 10.1016/j.stem.2012.11.011. Epub 2012 Dec 13. PubMed.

Description

Transcription activator-like effector nucleases (TALENs) are a new class of engineered nucleases that, due to their modular domain structure, have proven more straightforward to design and construct for performing genome editing than other types of nucleases. TALENs are typically designed as a pair that binds to genomic sequences flanking a target site and generates a double-strand break, which is repaired by the cell using either homology-directed repair (HDR) or the error-prone process of nonhomologous end-joining (NHEJ).

talen-musunuru-cowan-figure-1.png

The DNA-binding domain of a TALEN comprises an array of 34-amino-acid monomers that are ‘‘coded’’ to recognize and bind specific DNA base pairs (bp) in a 1:1 fashion. We have built upon previously described modular Golden Gate methodologies to allow assembly of multiple DNA fragments in an ordered fashion, such that a single ligation of preassembled tetramers and trimers generates TALENs that recognize any 15 bp recognition site in the genome.

This assembly method requires only 1-2 days for completion and is not prone to errors that complicate methods that rely on PCR amplification of monomers. Furthermore, we have developed a set of optimized vectors and methods for the delivery of TALENs into mammalian cells and, in particular, human pluripotent stem cells and other difficult-to-transfect cell types.

In brief, we transfect or electroporate TALEN pairs into cells and then subject them to fluorescence-activated cell sorting (FACS) 48 hr posttransfection based on fluorescent-marker expression. We replate the sorted cells at low density and allow them to recover and grow for 1 week, resulting in the formation of distinct single colonies. Colonies are expanded, genomic DNA purified, and mutations analyzed by PCR, agarose-gel screening, and Sanger sequencing. The entire process from start to finish can be completed in less than one month.

TALEN Illustration

To assemble a TALEN that recognizes the sequence N1-N2-N3-N4-N5-N6-N7-N8-N9-N10-N11-N12-N13-N14-N15, use the plasmids called I-N1-N2-N3-N4, II-N5-N6-N7-N8, III-N9-N10-N11-N12, and IV-N13-N14-N15. (It is optimal, but not essential, for the nucleotide preceding N1 to be T, as TALENs have an intrinsic preference for a T in the "N0" position.)

For example, to recognize the sequence 5'-GATGATCTCAGAGGC-3', use the plasmids called I-GATG, II-ATCT, III-CAGA, and IV-GGC. These plasmids can be ordered either individually or as part of the full 834-plasmid Kit.

The inserts from these plasmids are then assembled into either pTAL_GFP or pTAL_RFP via a single ligation reaction. To create a working TALEN pair, one of the TALENs should be assembled in pTAL_GFP and the other in pTAL_RFP, as the two pTAL plasmids encode distinct FokI nuclease domains that form an obligate heterodimer.

The full sequences for the pTAL_GFP and pTAL_RFP plasmids in GenBank format, as well as a spreadsheet with the insert sequences of the I/II/III/IV vectors, is available as a ZIP file (see "Protocols & Resources" tab).

Kit Documentation

Icon Musunuru/Cowan Lab TALEN Kit Info (93.9 KB)

Protocols

A detailed protocol for the assembly of TALENs with this Kit and their use to perform genome editing of cells is available at StemBook. A PDF of the protocol can be downloaded from that page.

Links

Icon Musunuru/Cowan Lab TALEN Kit Sequences (51.7 KB)

How to Cite this Kit

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which they were created, and include Addgene in the Materials and Methods of your future publications.

For your Materials and Methods section:
"The plasmid kit used for generation of TALENs was a gift from Kiran Musunuru and Chad Cowan (Addgene kit # 1000000034)"

For your Reference section:
A TALEN Genome-Editing System for Generating Human Stem Cell-Based Disease Models. Ding Q, Lee Y, Schaefer E, Peters D, Veres A, Kim K, Kuperwasser N, Motola D, Meissner T, Hendriks W, Trevisan M, Gupta R, Moisan A, Banks E, Friesen M, Schinzel R, Xia F, Tang A, Xia Y, Figueroa E, Wann A, Ahfeldt T, Daheron L, Zhang F, Rubin L, Peng L, Chung R, Musunuru K, Cowan C. Cell Stem Cell. 2013 Feb 7;12(2):238-51. doi: 10.1016/j.stem.2012.11.011. Epub 2012 Dec 13. PubMed.

Resistance Color Key:

 
  Ampicillin and Kanamycin  
 
  Ampicillin  

Inventory

Well Plasmid Resistance
1 / A / 1 I-AAAA
  Ampicillin and Kanamycin
1 / A / 2 I-AAAC
  Ampicillin and Kanamycin
1 / A / 3 I-AAAG
  Ampicillin and Kanamycin
1 / A / 4 I-AAAT
  Ampicillin and Kanamycin
1 / A / 5 I-AACA
  Ampicillin and Kanamycin
1 / A / 6 I-AACC
  Ampicillin and Kanamycin
1 / A / 7 I-AACG
  Ampicillin and Kanamycin
1 / A / 8 I-AACT
  Ampicillin and Kanamycin
1 / A / 9 I-AAGA
  Ampicillin and Kanamycin
1 / A / 10 I-AAGC
  Ampicillin and Kanamycin
1 / A / 11 I-AAGG
  Ampicillin and Kanamycin
1 / A / 12 I-AAGT
  Ampicillin and Kanamycin
1 / B / 1 I-AATA
  Ampicillin and Kanamycin
1 / B / 2 I-AATC
  Ampicillin and Kanamycin
1 / B / 3 I-AATG
  Ampicillin and Kanamycin
1 / B / 4 I-AATT
  Ampicillin and Kanamycin
1 / B / 5 I-ACAA
  Ampicillin and Kanamycin
1 / B / 6 I-ACAC
  Ampicillin and Kanamycin
1 / B / 7 I-ACAG
  Ampicillin and Kanamycin
1 / B / 8 I-ACAT
  Ampicillin and Kanamycin
1 / B / 9 I-ACCA
  Ampicillin and Kanamycin
1 / B / 10 I-ACCC
  Ampicillin and Kanamycin
1 / B / 11 I-ACCG
  Ampicillin and Kanamycin
1 / B / 12 I-ACCT
  Ampicillin and Kanamycin
1 / C / 1 I-ACGA
  Ampicillin and Kanamycin
1 / C / 2 I-ACGC
  Ampicillin and Kanamycin
1 / C / 3 I-ACGG
  Ampicillin and Kanamycin
1 / C / 4 I-ACGT
  Ampicillin and Kanamycin
1 / C / 5 I-ACTA
  Ampicillin and Kanamycin
1 / C / 6 I-ACTC
  Ampicillin and Kanamycin
1 / C / 7 I-ACTG
  Ampicillin and Kanamycin
1 / C / 8 I-ACTT
  Ampicillin and Kanamycin
1 / C / 9 I-AGAA
  Ampicillin and Kanamycin
1 / C / 10 I-AGAC
  Ampicillin and Kanamycin
1 / C / 11 I-AGAG
  Ampicillin and Kanamycin
1 / C / 12 I-AGAT
  Ampicillin and Kanamycin
1 / D / 1 I-AGCA
  Ampicillin and Kanamycin
1 / D / 2 I-AGCC
  Ampicillin and Kanamycin
1 / D / 3 I-AGCG
  Ampicillin and Kanamycin
1 / D / 4 I-AGCT
  Ampicillin and Kanamycin
1 / D / 5 I-AGGA
  Ampicillin and Kanamycin
1 / D / 6 I-AGGC
  Ampicillin and Kanamycin
1 / D / 7 I-AGGG
  Ampicillin and Kanamycin
1 / D / 8 I-AGGT
  Ampicillin and Kanamycin
1 / D / 9 I-AGTA
  Ampicillin and Kanamycin
1 / D / 10 I-AGTC
  Ampicillin and Kanamycin
1 / D / 11 I-AGTG
  Ampicillin and Kanamycin
1 / D / 12 I-AGTT
  Ampicillin and Kanamycin
1 / E / 1 I-ATAA
  Ampicillin and Kanamycin
1 / E / 2 I-ATAC
  Ampicillin and Kanamycin
1 / E / 3 I-ATAG
  Ampicillin and Kanamycin
1 / E / 4 I-ATAT
  Ampicillin and Kanamycin
1 / E / 5 I-ATCA
  Ampicillin and Kanamycin
1 / E / 6 I-ATCC
  Ampicillin and Kanamycin
1 / E / 7 I-ATCG
  Ampicillin and Kanamycin
1 / E / 8 I-ATCT
  Ampicillin and Kanamycin
1 / E / 9 I-ATGA
  Ampicillin and Kanamycin
1 / E / 10 I-ATGC
  Ampicillin and Kanamycin
1 / E / 11 I-ATGG
  Ampicillin and Kanamycin
1 / E / 12 I-ATGT
  Ampicillin and Kanamycin
1 / F / 1 I-ATTA
  Ampicillin and Kanamycin
1 / F / 2 I-ATTC
  Ampicillin and Kanamycin
1 / F / 3 I-ATTG
  Ampicillin and Kanamycin
1 / F / 4 I-ATTT
  Ampicillin and Kanamycin
1 / F / 5 I-CAAA
  Ampicillin and Kanamycin
1 / F / 6 I-CAAC
  Ampicillin and Kanamycin
1 / F / 7 I-CAAG
  Ampicillin and Kanamycin
1 / F / 8 I-CAAT
  Ampicillin and Kanamycin
1 / F / 9 I-CACA
  Ampicillin and Kanamycin
1 / F / 10 I-CACC
  Ampicillin and Kanamycin
1 / F / 11 I-CACG
  Ampicillin and Kanamycin
1 / F / 12 I-CACT
  Ampicillin and Kanamycin
1 / G / 1 I-CAGA
  Ampicillin and Kanamycin
1 / G / 2 I-CAGC
  Ampicillin and Kanamycin
1 / G / 3 I-CAGG
  Ampicillin and Kanamycin
1 / G / 4 I-CAGT
  Ampicillin and Kanamycin
1 / G / 5 I-CATA
  Ampicillin and Kanamycin
1 / G / 6 I-CATC
  Ampicillin and Kanamycin
1 / G / 7 I-CATG
  Ampicillin and Kanamycin
1 / G / 8 I-CATT
  Ampicillin and Kanamycin
1 / G / 9 I-CCAA
  Ampicillin and Kanamycin
1 / G / 10 I-CCAC
  Ampicillin and Kanamycin
1 / G / 11 I-CCAG
  Ampicillin and Kanamycin
1 / G / 12 I-CCAT
  Ampicillin and Kanamycin
1 / H / 1 I-CCCA
  Ampicillin and Kanamycin
1 / H / 2 I-CCCC
  Ampicillin and Kanamycin
1 / H / 3 I-CCCG
  Ampicillin and Kanamycin
1 / H / 4 I-CCCT
  Ampicillin and Kanamycin
1 / H / 5 I-CCGA
  Ampicillin and Kanamycin
1 / H / 6 I-CCGC
  Ampicillin and Kanamycin
1 / H / 7 I-CCGG
  Ampicillin and Kanamycin
1 / H / 8 I-CCGT
  Ampicillin and Kanamycin
1 / H / 9 I-CCTA
  Ampicillin and Kanamycin
1 / H / 10 I-CCTC
  Ampicillin and Kanamycin
1 / H / 11 I-CCTG
  Ampicillin and Kanamycin
1 / H / 12 I-CCTT
  Ampicillin and Kanamycin
2 / A / 1 I-CGAA
  Ampicillin and Kanamycin
2 / A / 2 I-CGAC
  Ampicillin and Kanamycin
2 / A / 3 I-CGAG
  Ampicillin and Kanamycin
2 / A / 4 I-CGAT
  Ampicillin and Kanamycin
2 / A / 5 I-CGCA
  Ampicillin and Kanamycin
2 / A / 6 I-CGCC
  Ampicillin and Kanamycin
2 / A / 7 I-CGCG
  Ampicillin and Kanamycin
2 / A / 8 I-CGCT
  Ampicillin and Kanamycin
2 / A / 9 I-CGGA
  Ampicillin and Kanamycin
2 / A / 10 I-CGGC
  Ampicillin and Kanamycin
2 / A / 11 I-CGGG
  Ampicillin and Kanamycin
2 / A / 12 I-CGGT
  Ampicillin and Kanamycin
2 / B / 1 I-CGTA
  Ampicillin and Kanamycin
2 / B / 2 I-CGTC
  Ampicillin and Kanamycin
2 / B / 3 I-CGTG
  Ampicillin and Kanamycin
2 / B / 4 I-CGTT
  Ampicillin and Kanamycin
2 / B / 5 I-CTAA
  Ampicillin and Kanamycin
2 / B / 6 I-CTAC
  Ampicillin and Kanamycin
2 / B / 7 I-CTAG
  Ampicillin and Kanamycin
2 / B / 8 I-CTAT
  Ampicillin and Kanamycin
2 / B / 9 I-CTCA
  Ampicillin and Kanamycin
2 / B / 10 I-CTCC
  Ampicillin and Kanamycin
2 / B / 11 I-CTCG
  Ampicillin and Kanamycin
2 / B / 12 I-CTCT
  Ampicillin and Kanamycin
2 / C / 1 I-CTGA
  Ampicillin and Kanamycin
2 / C / 2 I-CTGC
  Ampicillin and Kanamycin
2 / C / 3 I-CTGG
  Ampicillin and Kanamycin
2 / C / 4 I-CTGT
  Ampicillin and Kanamycin
2 / C / 5 I-CTTA
  Ampicillin and Kanamycin
2 / C / 6 I-CTTC
  Ampicillin and Kanamycin
2 / C / 7 I-CTTG
  Ampicillin and Kanamycin
2 / C / 8 I-CTTT
  Ampicillin and Kanamycin
2 / C / 9 I-GAAA
  Ampicillin and Kanamycin
2 / C / 10 I-GAAC
  Ampicillin and Kanamycin
2 / C / 11 I-GAAG
  Ampicillin and Kanamycin
2 / C / 12 I-GAAT
  Ampicillin and Kanamycin
2 / D / 1 I-GACA
  Ampicillin and Kanamycin
2 / D / 2 I-GACC
  Ampicillin and Kanamycin
2 / D / 3 I-GACG
  Ampicillin and Kanamycin
2 / D / 4 I-GACT
  Ampicillin and Kanamycin
2 / D / 5 I-GAGA
  Ampicillin and Kanamycin
2 / D / 6 I-GAGC
  Ampicillin and Kanamycin
2 / D / 7 I-GAGG
  Ampicillin and Kanamycin
2 / D / 8 I-GAGT
  Ampicillin and Kanamycin
2 / D / 9 I-GATA
  Ampicillin and Kanamycin
2 / D / 10 I-GATC
  Ampicillin and Kanamycin
2 / D / 11 I-GATG
  Ampicillin and Kanamycin
2 / D / 12 I-GATT
  Ampicillin and Kanamycin
2 / E / 1 I-GCAA
  Ampicillin and Kanamycin
2 / E / 2 I-GCAC
  Ampicillin and Kanamycin
2 / E / 3 I-GCAG
  Ampicillin and Kanamycin
2 / E / 4 I-GCAT
  Ampicillin and Kanamycin
2 / E / 5 I-GCCA
  Ampicillin and Kanamycin
2 / E / 6 I-GCCC
  Ampicillin and Kanamycin
2 / E / 7 I-GCCG
  Ampicillin and Kanamycin
2 / E / 8 I-GCCT
  Ampicillin and Kanamycin
2 / E / 9 I-GCGA
  Ampicillin and Kanamycin
2 / E / 10 I-GCGC
  Ampicillin and Kanamycin
2 / E / 11 I-GCGG
  Ampicillin and Kanamycin
2 / E / 12 I-GCGT
  Ampicillin and Kanamycin
2 / F / 1 I-GCTA
  Ampicillin and Kanamycin
2 / F / 2 I-GCTC
  Ampicillin and Kanamycin
2 / F / 3 I-GCTG
  Ampicillin and Kanamycin
2 / F / 4 I-GCTT
  Ampicillin and Kanamycin
2 / F / 5 I-GGAA
  Ampicillin and Kanamycin
2 / F / 6 I-GGAC
  Ampicillin and Kanamycin
2 / F / 7 I-GGAG
  Ampicillin and Kanamycin
2 / F / 8 I-GGAT
  Ampicillin and Kanamycin
2 / F / 9 I-GGCA
  Ampicillin and Kanamycin
2 / F / 10 I-GGCC
  Ampicillin and Kanamycin
2 / F / 11 I-GGCG
  Ampicillin and Kanamycin
2 / F / 12 I-GGCT
  Ampicillin and Kanamycin
2 / G / 1 I-GGGA
  Ampicillin and Kanamycin
2 / G / 2 I-GGGC
  Ampicillin and Kanamycin
2 / G / 3 I-GGGG
  Ampicillin and Kanamycin
2 / G / 4 I-GGGT
  Ampicillin and Kanamycin
2 / G / 5 I-GGTA
  Ampicillin and Kanamycin
2 / G / 6 I-GGTC
  Ampicillin and Kanamycin
2 / G / 7 I-GGTG
  Ampicillin and Kanamycin
2 / G / 8 I-GGTT
  Ampicillin and Kanamycin
2 / G / 9 I-GTAA
  Ampicillin and Kanamycin
2 / G / 10 I-GTAC
  Ampicillin and Kanamycin
2 / G / 11 I-GTAG
  Ampicillin and Kanamycin
2 / G / 12 I-GTAT
  Ampicillin and Kanamycin
2 / H / 1 I-GTCA
  Ampicillin and Kanamycin
2 / H / 2 I-GTCC
  Ampicillin and Kanamycin
2 / H / 3 I-GTCG
  Ampicillin and Kanamycin
2 / H / 4 I-GTCT
  Ampicillin and Kanamycin
2 / H / 5 I-GTGA
  Ampicillin and Kanamycin
2 / H / 6 I-GTGC
  Ampicillin and Kanamycin
2 / H / 7 I-GTGG
  Ampicillin and Kanamycin
2 / H / 8 I-GTGT
  Ampicillin and Kanamycin
2 / H / 9 I-GTTA
  Ampicillin and Kanamycin
2 / H / 10 I-GTTC
  Ampicillin and Kanamycin
2 / H / 11 I-GTTG
  Ampicillin and Kanamycin
2 / H / 12 I-GTTT
  Ampicillin and Kanamycin
3 / A / 1 I-TAAA
  Ampicillin and Kanamycin
3 / A / 2 I-TAAC
  Ampicillin and Kanamycin
3 / A / 3 I-TAAG
  Ampicillin and Kanamycin
3 / A / 4 I-TAAT
  Ampicillin and Kanamycin
3 / A / 5 I-TACA
  Ampicillin and Kanamycin
3 / A / 6 I-TACC
  Ampicillin and Kanamycin
3 / A / 7 I-TACG
  Ampicillin and Kanamycin
3 / A / 8 I-TACT
  Ampicillin and Kanamycin
3 / A / 9 I-TAGA
  Ampicillin and Kanamycin
3 / A / 10 I-TAGC
  Ampicillin and Kanamycin
3 / A / 11 I-TAGG
  Ampicillin and Kanamycin
3 / A / 12 I-TAGT
  Ampicillin and Kanamycin
3 / B / 1 I-TATA
  Ampicillin and Kanamycin
3 / B / 2 I-TATC
  Ampicillin and Kanamycin
3 / B / 3 I-TATG
  Ampicillin and Kanamycin
3 / B / 4 I-TATT
  Ampicillin and Kanamycin
3 / B / 5 I-TCAA
  Ampicillin and Kanamycin
3 / B / 6 I-TCAC
  Ampicillin and Kanamycin
3 / B / 7 I-TCAG
  Ampicillin and Kanamycin
3 / B / 8 I-TCAT
  Ampicillin and Kanamycin
3 / B / 9 I-TCCA
  Ampicillin and Kanamycin
3 / B / 10 I-TCCC
  Ampicillin and Kanamycin
3 / B / 11 I-TCCG
  Ampicillin and Kanamycin
3 / B / 12 I-TCCT
  Ampicillin and Kanamycin
3 / C / 1 I-TCGA
  Ampicillin and Kanamycin
3 / C / 2 I-TCGC
  Ampicillin and Kanamycin
3 / C / 3 I-TCGG
  Ampicillin and Kanamycin
3 / C / 4 I-TCGT
  Ampicillin and Kanamycin
3 / C / 5 I-TCTA
  Ampicillin and Kanamycin
3 / C / 6 I-TCTC
  Ampicillin and Kanamycin
3 / C / 7 I-TCTG
  Ampicillin and Kanamycin
3 / C / 8 I-TCTT
  Ampicillin and Kanamycin
3 / C / 9 I-TGAA
  Ampicillin and Kanamycin
3 / C / 10 I-TGAC
  Ampicillin and Kanamycin
3 / C / 11 I-TGAG
  Ampicillin and Kanamycin
3 / C / 12 I-TGAT
  Ampicillin and Kanamycin
3 / D / 1 I-TGCA
  Ampicillin and Kanamycin
3 / D / 2 I-TGCC
  Ampicillin and Kanamycin
3 / D / 3 I-TGCG
  Ampicillin and Kanamycin
3 / D / 4 I-TGCT
  Ampicillin and Kanamycin
3 / D / 5 I-TGGA
  Ampicillin and Kanamycin
3 / D / 6 I-TGGC
  Ampicillin and Kanamycin
3 / D / 7 I-TGGG
  Ampicillin and Kanamycin
3 / D / 8 I-TGGT
  Ampicillin and Kanamycin
3 / D / 9 I-TGTA
  Ampicillin and Kanamycin
3 / D / 10 I-TGTC
  Ampicillin and Kanamycin
3 / D / 11 I-TGTG
  Ampicillin and Kanamycin
3 / D / 12 I-TGTT
  Ampicillin and Kanamycin
3 / E / 1 I-TTAA
  Ampicillin and Kanamycin
3 / E / 2 I-TTAC
  Ampicillin and Kanamycin
3 / E / 3 I-TTAG
  Ampicillin and Kanamycin
3 / E / 4 I-TTAT
  Ampicillin and Kanamycin
3 / E / 5 I-TTCA
  Ampicillin and Kanamycin
3 / E / 6 I-TTCC
  Ampicillin and Kanamycin
3 / E / 7 I-TTCG
  Ampicillin and Kanamycin
3 / E / 8 I-TTCT
  Ampicillin and Kanamycin
3 / E / 9 I-TTGA
  Ampicillin and Kanamycin
3 / E / 10 I-TTGC
  Ampicillin and Kanamycin
3 / E / 11 I-TTGG
  Ampicillin and Kanamycin
3 / E / 12 I-TTGT
  Ampicillin and Kanamycin
3 / F / 1 I-TTTA
  Ampicillin and Kanamycin
3 / F / 2 I-TTTC
  Ampicillin and Kanamycin
3 / F / 3 I-TTTG
  Ampicillin and Kanamycin
3 / F / 4 I-TTTT
  Ampicillin and Kanamycin
3 / F / 5 II-AAAA
  Ampicillin and Kanamycin
3 / F / 6 II-AAAC
  Ampicillin and Kanamycin
3 / F / 7 II-AAAG
  Ampicillin and Kanamycin
3 / F / 8 II-AAAT
  Ampicillin and Kanamycin
3 / F / 9 II-AACA
  Ampicillin and Kanamycin
3 / F / 10 II-AACC
  Ampicillin and Kanamycin
3 / F / 11 II-AACG
  Ampicillin and Kanamycin
3 / F / 12 II-AACT
  Ampicillin and Kanamycin
3 / G / 1 II-AAGA
  Ampicillin and Kanamycin
3 / G / 2 II-AAGC
  Ampicillin and Kanamycin
3 / G / 3 II-AAGG
  Ampicillin and Kanamycin
3 / G / 4 II-AAGT
  Ampicillin and Kanamycin
3 / G / 5 II-AATA
  Ampicillin and Kanamycin
3 / G / 6 II-AATC
  Ampicillin and Kanamycin
3 / G / 7 II-AATG
  Ampicillin and Kanamycin
3 / G / 8 II-AATT
  Ampicillin and Kanamycin
3 / G / 9 II-ACAA
  Ampicillin and Kanamycin
3 / G / 10 II-ACAC
  Ampicillin and Kanamycin
3 / G / 11 II-ACAG
  Ampicillin and Kanamycin
3 / G / 12 II-ACAT
  Ampicillin and Kanamycin
3 / H / 1 II-ACCA
  Ampicillin and Kanamycin
3 / H / 2 II-ACCC
  Ampicillin and Kanamycin
3 / H / 3 II-ACCG
  Ampicillin and Kanamycin
3 / H / 4 II-ACCT
  Ampicillin and Kanamycin
3 / H / 5 II-ACGA
  Ampicillin and Kanamycin
3 / H / 6 II-ACGC
  Ampicillin and Kanamycin
3 / H / 7 II-ACGG
  Ampicillin and Kanamycin
3 / H / 8 II-ACGT
  Ampicillin and Kanamycin
3 / H / 9 II-ACTA
  Ampicillin and Kanamycin
3 / H / 10 II-ACTC
  Ampicillin and Kanamycin
3 / H / 11 II-ACTG
  Ampicillin and Kanamycin
3 / H / 12 II-ACTT
  Ampicillin and Kanamycin
4 / A / 1 II-AGAA
  Ampicillin and Kanamycin
4 / A / 2 II-AGAC
  Ampicillin and Kanamycin
4 / A / 3 II-AGAG
  Ampicillin and Kanamycin
4 / A / 4 II-AGAT
  Ampicillin and Kanamycin
4 / A / 5 II-AGCA
  Ampicillin and Kanamycin
4 / A / 6 II-AGCC
  Ampicillin and Kanamycin
4 / A / 7 II-AGCG
  Ampicillin and Kanamycin
4 / A / 8 II-AGCT
  Ampicillin and Kanamycin
4 / A / 9 II-AGGA
  Ampicillin and Kanamycin
4 / A / 10 II-AGGC
  Ampicillin and Kanamycin
4 / A / 11 II-AGGG
  Ampicillin and Kanamycin
4 / A / 12 II-AGGT
  Ampicillin and Kanamycin
4 / B / 1 II-AGTA
  Ampicillin and Kanamycin
4 / B / 2 II-AGTC
  Ampicillin and Kanamycin
4 / B / 3 II-AGTG
  Ampicillin and Kanamycin
4 / B / 4 II-AGTT
  Ampicillin and Kanamycin
4 / B / 5 II-ATAA
  Ampicillin and Kanamycin
4 / B / 6 II-ATAC
  Ampicillin and Kanamycin
4 / B / 7 II-ATAG
  Ampicillin and Kanamycin
4 / B / 8 II-ATAT
  Ampicillin and Kanamycin
4 / B / 9 II-ATCA
  Ampicillin and Kanamycin
4 / B / 10 II-ATCC
  Ampicillin and Kanamycin
4 / B / 11 II-ATCG
  Ampicillin and Kanamycin
4 / B / 12 II-ATCT
  Ampicillin and Kanamycin
4 / C / 1 II-ATGA
  Ampicillin and Kanamycin
4 / C / 2 II-ATGC
  Ampicillin and Kanamycin
4 / C / 3 II-ATGG
  Ampicillin and Kanamycin
4 / C / 4 II-ATGT
  Ampicillin and Kanamycin
4 / C / 5 II-ATTA
  Ampicillin and Kanamycin
4 / C / 6 II-ATTC
  Ampicillin and Kanamycin
4 / C / 7 II-ATTG
  Ampicillin and Kanamycin
4 / C / 8 II-ATTT
  Ampicillin and Kanamycin
4 / C / 9 II-CAAA
  Ampicillin and Kanamycin
4 / C / 10 II-CAAC
  Ampicillin and Kanamycin
4 / C / 11 II-CAAG
  Ampicillin and Kanamycin
4 / C / 12 II-CAAT
  Ampicillin and Kanamycin
4 / D / 1 II-CACA
  Ampicillin and Kanamycin
4 / D / 2 II-CACC
  Ampicillin and Kanamycin
4 / D / 3 II-CACG
  Ampicillin and Kanamycin
4 / D / 4 II-CACT
  Ampicillin and Kanamycin
4 / D / 5 II-CAGA
  Ampicillin and Kanamycin
4 / D / 6 II-CAGC
  Ampicillin and Kanamycin
4 / D / 7 II-CAGG
  Ampicillin and Kanamycin
4 / D / 8 II-CAGT
  Ampicillin and Kanamycin
4 / D / 9 II-CATA
  Ampicillin and Kanamycin
4 / D / 10 II-CATC
  Ampicillin and Kanamycin
4 / D / 11 II-CATG
  Ampicillin and Kanamycin
4 / D / 12 II-CATT
  Ampicillin and Kanamycin
4 / E / 1 II-CCAA
  Ampicillin and Kanamycin
4 / E / 2 II-CCAC
  Ampicillin and Kanamycin
4 / E / 3 II-CCAG
  Ampicillin and Kanamycin
4 / E / 4 II-CCAT
  Ampicillin and Kanamycin
4 / E / 5 II-CCCA
  Ampicillin and Kanamycin
4 / E / 6 II-CCCC
  Ampicillin and Kanamycin
4 / E / 7 II-CCCG
  Ampicillin and Kanamycin
4 / E / 8 II-CCCT
  Ampicillin and Kanamycin
4 / E / 9 II-CCGA
  Ampicillin and Kanamycin
4 / E / 10 II-CCGC
  Ampicillin and Kanamycin
4 / E / 11 II-CCGG
  Ampicillin and Kanamycin
4 / E / 12 II-CCGT
  Ampicillin and Kanamycin
4 / F / 1 II-CCTA
  Ampicillin and Kanamycin
4 / F / 2 II-CCTC
  Ampicillin and Kanamycin
4 / F / 3 II-CCTG
  Ampicillin and Kanamycin
4 / F / 4 II-CCTT
  Ampicillin and Kanamycin
4 / F / 5 II-CGAA
  Ampicillin and Kanamycin
4 / F / 6 II-CGAC
  Ampicillin and Kanamycin
4 / F / 7 II-CGAG
  Ampicillin and Kanamycin
4 / F / 8 II-CGAT
  Ampicillin and Kanamycin
4 / F / 9 II-CGCA
  Ampicillin and Kanamycin
4 / F / 10 II-CGCC
  Ampicillin and Kanamycin
4 / F / 11 II-CGCG
  Ampicillin and Kanamycin
4 / F / 12 II-CGCT
  Ampicillin and Kanamycin
4 / G / 1 II-CGGA
  Ampicillin and Kanamycin
4 / G / 2 II-CGGC
  Ampicillin and Kanamycin
4 / G / 3 II-CGGG
  Ampicillin and Kanamycin
4 / G / 4 II-CGGT
  Ampicillin and Kanamycin
4 / G / 5 II-CGTA
  Ampicillin and Kanamycin
4 / G / 6 II-CGTC
  Ampicillin and Kanamycin
4 / G / 7 II-CGTG
  Ampicillin and Kanamycin
4 / G / 8 II-CGTT
  Ampicillin and Kanamycin
4 / G / 9 II-CTAA
  Ampicillin and Kanamycin
4 / G / 10 II-CTAC
  Ampicillin and Kanamycin
4 / G / 11 II-CTAG
  Ampicillin and Kanamycin
4 / G / 12 II-CTAT
  Ampicillin and Kanamycin
4 / H / 1 II-CTCA
  Ampicillin and Kanamycin
4 / H / 2 II-CTCC
  Ampicillin and Kanamycin
4 / H / 3 II-CTCG
  Ampicillin and Kanamycin
4 / H / 4 II-CTCT
  Ampicillin and Kanamycin
4 / H / 5 II-CTGA
  Ampicillin and Kanamycin
4 / H / 6 II-CTGC
  Ampicillin and Kanamycin
4 / H / 7 II-CTGG
  Ampicillin and Kanamycin
4 / H / 8 II-CTGT
  Ampicillin and Kanamycin
4 / H / 9 II-CTTA
  Ampicillin and Kanamycin
4 / H / 10 II-CTTC
  Ampicillin and Kanamycin
4 / H / 11 II-CTTG
  Ampicillin and Kanamycin
4 / H / 12 II-CTTT
  Ampicillin and Kanamycin
5 / A / 1 II-GAAA
  Ampicillin and Kanamycin
5 / A / 2 II-GAAC
  Ampicillin and Kanamycin
5 / A / 3 II-GAAG
  Ampicillin and Kanamycin
5 / A / 4 II-GAAT
  Ampicillin and Kanamycin
5 / A / 5 II-GACA
  Ampicillin and Kanamycin
5 / A / 6 II-GACC
  Ampicillin and Kanamycin
5 / A / 7 II-GACG
  Ampicillin and Kanamycin
5 / A / 8 II-GACT
  Ampicillin and Kanamycin
5 / A / 9 II-GAGA
  Ampicillin and Kanamycin
5 / A / 10 II-GAGC
  Ampicillin and Kanamycin
5 / A / 11 II-GAGG
  Ampicillin and Kanamycin
5 / A / 12 II-GAGT
  Ampicillin and Kanamycin
5 / B / 1 II-GATA
  Ampicillin and Kanamycin
5 / B / 2 II-GATC
  Ampicillin and Kanamycin
5 / B / 3 II-GATG
  Ampicillin and Kanamycin
5 / B / 4 II-GATT
  Ampicillin and Kanamycin
5 / B / 5 II-GCAA
  Ampicillin and Kanamycin
5 / B / 6 II-GCAC
  Ampicillin and Kanamycin
5 / B / 7 II-GCAG
  Ampicillin and Kanamycin
5 / B / 8 II-GCAT
  Ampicillin and Kanamycin
5 / B / 9 II-GCCA
  Ampicillin and Kanamycin
5 / B / 10 II-GCCC
  Ampicillin and Kanamycin
5 / B / 11 II-GCCG
  Ampicillin and Kanamycin
5 / B / 12 II-GCCT
  Ampicillin and Kanamycin
5 / C / 1 II-GCGA
  Ampicillin and Kanamycin
5 / C / 2 II-GCGC
  Ampicillin and Kanamycin
5 / C / 3 II-GCGG
  Ampicillin and Kanamycin
5 / C / 4 II-GCGT
  Ampicillin and Kanamycin
5 / C / 5 II-GCTA
  Ampicillin and Kanamycin
5 / C / 6 II-GCTC
  Ampicillin and Kanamycin
5 / C / 7 II-GCTG
  Ampicillin and Kanamycin
5 / C / 8 II-GCTT
  Ampicillin and Kanamycin
5 / C / 9 II-GGAA
  Ampicillin and Kanamycin
5 / C / 10 II-GGAC
  Ampicillin and Kanamycin
5 / C / 11 II-GGAG
  Ampicillin and Kanamycin
5 / C / 12 II-GGAT
  Ampicillin and Kanamycin
5 / D / 1 II-GGCA
  Ampicillin and Kanamycin
5 / D / 2 II-GGCC
  Ampicillin and Kanamycin
5 / D / 3 II-GGCG
  Ampicillin and Kanamycin
5 / D / 4 II-GGCT
  Ampicillin and Kanamycin
5 / D / 5 II-GGGA
  Ampicillin and Kanamycin
5 / D / 6 II-GGGC
  Ampicillin and Kanamycin
5 / D / 7 II-GGGG
  Ampicillin and Kanamycin
5 / D / 8 II-GGGT
  Ampicillin and Kanamycin
5 / D / 9 II-GGTA
  Ampicillin and Kanamycin
5 / D / 10 II-GGTC
  Ampicillin and Kanamycin
5 / D / 11 II-GGTG
  Ampicillin and Kanamycin
5 / D / 12 II-GGTT
  Ampicillin and Kanamycin
5 / E / 1 II-GTAA
  Ampicillin and Kanamycin
5 / E / 2 II-GTAC
  Ampicillin and Kanamycin
5 / E / 3 II-GTAG
  Ampicillin and Kanamycin
5 / E / 4 II-GTAT
  Ampicillin and Kanamycin
5 / E / 5 II-GTCA
  Ampicillin and Kanamycin
5 / E / 6 II-GTCC
  Ampicillin and Kanamycin
5 / E / 7 II-GTCG
  Ampicillin and Kanamycin
5 / E / 8 II-GTCT
  Ampicillin and Kanamycin
5 / E / 9 II-GTGA
  Ampicillin and Kanamycin
5 / E / 10 II-GTGC
  Ampicillin and Kanamycin
5 / E / 11 II-GTGG
  Ampicillin and Kanamycin
5 / E / 12 II-GTGT
  Ampicillin and Kanamycin
5 / F / 1 II-GTTA
  Ampicillin and Kanamycin
5 / F / 2 II-GTTC
  Ampicillin and Kanamycin
5 / F / 3 II-GTTG
  Ampicillin and Kanamycin
5 / F / 4 II-GTTT
  Ampicillin and Kanamycin
5 / F / 5 II-TAAA
  Ampicillin and Kanamycin
5 / F / 6 II-TAAC
  Ampicillin and Kanamycin
5 / F / 7 II-TAAG
  Ampicillin and Kanamycin
5 / F / 8 II-TAAT
  Ampicillin and Kanamycin
5 / F / 9 II-TACA
  Ampicillin and Kanamycin
5 / F / 10 II-TACC
  Ampicillin and Kanamycin
5 / F / 11 II-TACG
  Ampicillin and Kanamycin
5 / F / 12 II-TACT
  Ampicillin and Kanamycin
5 / G / 1 II-TAGA
  Ampicillin and Kanamycin
5 / G / 2 II-TAGC
  Ampicillin and Kanamycin
5 / G / 3 II-TAGG
  Ampicillin and Kanamycin
5 / G / 4 II-TAGT
  Ampicillin and Kanamycin
5 / G / 5 II-TATA
  Ampicillin and Kanamycin
5 / G / 6 II-TATC
  Ampicillin and Kanamycin
5 / G / 7 II-TATG
  Ampicillin and Kanamycin
5 / G / 8 II-TATT
  Ampicillin and Kanamycin
5 / G / 9 II-TCAA
  Ampicillin and Kanamycin
5 / G / 10 II-TCAC
  Ampicillin and Kanamycin
5 / G / 11 II-TCAG
  Ampicillin and Kanamycin
5 / G / 12 II-TCAT
  Ampicillin and Kanamycin
5 / H / 1 II-TCCA
  Ampicillin and Kanamycin
5 / H / 2 II-TCCC
  Ampicillin and Kanamycin
5 / H / 3 II-TCCG
  Ampicillin and Kanamycin
5 / H / 4 II-TCCT
  Ampicillin and Kanamycin
5 / H / 5 II-TCGA
  Ampicillin and Kanamycin
5 / H / 6 II-TCGC
  Ampicillin and Kanamycin
5 / H / 7 II-TCGG
  Ampicillin and Kanamycin
5 / H / 8 II-TCGT
  Ampicillin and Kanamycin
5 / H / 9 II-TCTA
  Ampicillin and Kanamycin
5 / H / 10 II-TCTC
  Ampicillin and Kanamycin
5 / H / 11 II-TCTG
  Ampicillin and Kanamycin
5 / H / 12 II-TCTT
  Ampicillin and Kanamycin
6 / A / 1 II-TGAA
  Ampicillin and Kanamycin
6 / A / 2 II-TGAC
  Ampicillin and Kanamycin
6 / A / 3 II-TGAG
  Ampicillin and Kanamycin
6 / A / 4 II-TGAT
  Ampicillin and Kanamycin
6 / A / 5 II-TGCA
  Ampicillin and Kanamycin
6 / A / 6 II-TGCC
  Ampicillin and Kanamycin
6 / A / 7 II-TGCG
  Ampicillin and Kanamycin
6 / A / 8 II-TGCT
  Ampicillin and Kanamycin
6 / A / 9 II-TGGA
  Ampicillin and Kanamycin
6 / A / 10 II-TGGC
  Ampicillin and Kanamycin
6 / A / 11 II-TGGG
  Ampicillin and Kanamycin
6 / A / 12 II-TGGT
  Ampicillin and Kanamycin
6 / B / 1 II-TGTA
  Ampicillin and Kanamycin
6 / B / 2 II-TGTC
  Ampicillin and Kanamycin
6 / B / 3 II-TGTG
  Ampicillin and Kanamycin
6 / B / 4 II-TGTT
  Ampicillin and Kanamycin
6 / B / 5 II-TTAA
  Ampicillin and Kanamycin
6 / B / 6 II-TTAC
  Ampicillin and Kanamycin
6 / B / 7 II-TTAG
  Ampicillin and Kanamycin
6 / B / 8 II-TTAT
  Ampicillin and Kanamycin
6 / B / 9 II-TTCA
  Ampicillin and Kanamycin
6 / B / 10 II-TTCC
  Ampicillin and Kanamycin
6 / B / 11 II-TTCG
  Ampicillin and Kanamycin
6 / B / 12 II-TTCT
  Ampicillin and Kanamycin
6 / C / 1 II-TTGA
  Ampicillin and Kanamycin
6 / C / 2 II-TTGC
  Ampicillin and Kanamycin
6 / C / 3 II-TTGG
  Ampicillin and Kanamycin
6 / C / 4 II-TTGT
  Ampicillin and Kanamycin
6 / C / 5 II-TTTA
  Ampicillin and Kanamycin
6 / C / 6 II-TTTC
  Ampicillin and Kanamycin
6 / C / 7 II-TTTG
  Ampicillin and Kanamycin
6 / C / 8 II-TTTT
  Ampicillin and Kanamycin
6 / C / 9 III-AAAA
  Ampicillin and Kanamycin
6 / C / 10 III-AAAC
  Ampicillin and Kanamycin
6 / C / 11 III-AAAG
  Ampicillin and Kanamycin
6 / C / 12 III-AAAT
  Ampicillin and Kanamycin
6 / D / 1 III-AACA
  Ampicillin and Kanamycin
6 / D / 2 III-AACC
  Ampicillin and Kanamycin
6 / D / 3 III-AACG
  Ampicillin and Kanamycin
6 / D / 4 III-AACT
  Ampicillin and Kanamycin
6 / D / 5 III-AAGA
  Ampicillin and Kanamycin
6 / D / 6 III-AAGC
  Ampicillin and Kanamycin
6 / D / 7 III-AAGG
  Ampicillin and Kanamycin
6 / D / 8 III-AAGT
  Ampicillin and Kanamycin
6 / D / 9 III-AATA
  Ampicillin and Kanamycin
6 / D / 10 III-AATC
  Ampicillin and Kanamycin
6 / D / 11 III-AATG
  Ampicillin and Kanamycin
6 / D / 12 III-AATT
  Ampicillin and Kanamycin
6 / E / 1 III-ACAA
  Ampicillin and Kanamycin
6 / E / 2 III-ACAC
  Ampicillin and Kanamycin
6 / E / 3 III-ACAG
  Ampicillin and Kanamycin
6 / E / 4 III-ACAT
  Ampicillin and Kanamycin
6 / E / 5 III-ACCA
  Ampicillin and Kanamycin
6 / E / 6 III-ACCC
  Ampicillin and Kanamycin
6 / E / 7 III-ACCG
  Ampicillin and Kanamycin
6 / E / 8 III-ACCT
  Ampicillin and Kanamycin
6 / E / 9 III-ACGA
  Ampicillin and Kanamycin
6 / E / 10 III-ACGC
  Ampicillin and Kanamycin
6 / E / 11 III-ACGG
  Ampicillin and Kanamycin
6 / E / 12 III-ACGT
  Ampicillin and Kanamycin
6 / F / 1 III-ACTA
  Ampicillin and Kanamycin
6 / F / 2 III-ACTC
  Ampicillin and Kanamycin
6 / F / 3 III-ACTG
  Ampicillin and Kanamycin
6 / F / 4 III-ACTT
  Ampicillin and Kanamycin
6 / F / 5 III-AGAA
  Ampicillin and Kanamycin
6 / F / 6 III-AGAC
  Ampicillin and Kanamycin
6 / F / 7 III-AGAG
  Ampicillin and Kanamycin
6 / F / 8 III-AGAT
  Ampicillin and Kanamycin
6 / F / 9 III-AGCA
  Ampicillin and Kanamycin
6 / F / 10 III-AGCC
  Ampicillin and Kanamycin
6 / F / 11 III-AGCG
  Ampicillin and Kanamycin
6 / F / 12 III-AGCT
  Ampicillin and Kanamycin
6 / G / 1 III-AGGA
  Ampicillin and Kanamycin
6 / G / 2 III-AGGC
  Ampicillin and Kanamycin
6 / G / 3 III-AGGG
  Ampicillin and Kanamycin
6 / G / 4 III-AGGT
  Ampicillin and Kanamycin
6 / G / 5 III-AGTA
  Ampicillin and Kanamycin
6 / G / 6 III-AGTC
  Ampicillin and Kanamycin
6 / G / 7 III-AGTG
  Ampicillin and Kanamycin
6 / G / 8 III-AGTT
  Ampicillin and Kanamycin
6 / G / 9 III-ATAA
  Ampicillin and Kanamycin
6 / G / 10 III-ATAC
  Ampicillin and Kanamycin
6 / G / 11 III-ATAG
  Ampicillin and Kanamycin
6 / G / 12 III-ATAT
  Ampicillin and Kanamycin
6 / H / 1 III-ATCA
  Ampicillin and Kanamycin
6 / H / 2 III-ATCC
  Ampicillin and Kanamycin
6 / H / 3 III-ATCG
  Ampicillin and Kanamycin
6 / H / 4 III-ATCT
  Ampicillin and Kanamycin
6 / H / 5 III-ATGA
  Ampicillin and Kanamycin
6 / H / 6 III-ATGC
  Ampicillin and Kanamycin
6 / H / 7 III-ATGG
  Ampicillin and Kanamycin
6 / H / 8 III-ATGT
  Ampicillin and Kanamycin
6 / H / 9 III-ATTA
  Ampicillin and Kanamycin
6 / H / 10 III-ATTC
  Ampicillin and Kanamycin
6 / H / 11 III-ATTG
  Ampicillin and Kanamycin
6 / H / 12 III-ATTT
  Ampicillin and Kanamycin
7 / A / 1 III-CAAA
  Ampicillin and Kanamycin
7 / A / 2 III-CAAC
  Ampicillin and Kanamycin
7 / A / 3 III-CAAG
  Ampicillin and Kanamycin
7 / A / 4 III-CAAT
  Ampicillin and Kanamycin
7 / A / 5 III-CACA
  Ampicillin and Kanamycin
7 / A / 6 III-CACC
  Ampicillin and Kanamycin
7 / A / 7 III-CACG
  Ampicillin and Kanamycin
7 / A / 8 III-CACT
  Ampicillin and Kanamycin
7 / A / 9 III-CAGA
  Ampicillin and Kanamycin
7 / A / 10 III-CAGC
  Ampicillin and Kanamycin
7 / A / 11 III-CAGG
  Ampicillin and Kanamycin
7 / A / 12 III-CAGT
  Ampicillin and Kanamycin
7 / B / 1 III-CATA
  Ampicillin and Kanamycin
7 / B / 2 III-CATC
  Ampicillin and Kanamycin
7 / B / 3 III-CATG
  Ampicillin and Kanamycin
7 / B / 4 III-CATT
  Ampicillin and Kanamycin
7 / B / 5 III-CCAA
  Ampicillin and Kanamycin
7 / B / 6 III-CCAC
  Ampicillin and Kanamycin
7 / B / 7 III-CCAG
  Ampicillin and Kanamycin
7 / B / 8 III-CCAT
  Ampicillin and Kanamycin
7 / B / 9 III-CCCA
  Ampicillin and Kanamycin
7 / B / 10 III-CCCC
  Ampicillin and Kanamycin
7 / B / 11 III-CCCG
  Ampicillin and Kanamycin
7 / B / 12 III-CCCT
  Ampicillin and Kanamycin
7 / C / 1 III-CCGA
  Ampicillin and Kanamycin
7 / C / 2 III-CCGC
  Ampicillin and Kanamycin
7 / C / 3 III-CCGG
  Ampicillin and Kanamycin
7 / C / 4 III-CCGT
  Ampicillin and Kanamycin
7 / C / 5 III-CCTA
  Ampicillin and Kanamycin
7 / C / 6 III-CCTC
  Ampicillin and Kanamycin
7 / C / 7 III-CCTG
  Ampicillin and Kanamycin
7 / C / 8 III-CCTT
  Ampicillin and Kanamycin
7 / C / 9 III-CGAA
  Ampicillin and Kanamycin
7 / C / 10 III-CGAC
  Ampicillin and Kanamycin
7 / C / 11 III-CGAG
  Ampicillin and Kanamycin
7 / C / 12 III-CGAT
  Ampicillin and Kanamycin
7 / D / 1 III-CGCA
  Ampicillin and Kanamycin
7 / D / 2 III-CGCC
  Ampicillin and Kanamycin
7 / D / 3 III-CGCG
  Ampicillin and Kanamycin
7 / D / 4 III-CGCT
  Ampicillin and Kanamycin
7 / D / 5 III-CGGA
  Ampicillin and Kanamycin
7 / D / 6 III-CGGC
  Ampicillin and Kanamycin
7 / D / 7 III-CGGG
  Ampicillin and Kanamycin
7 / D / 8 III-CGGT
  Ampicillin and Kanamycin
7 / D / 9 III-CGTA
  Ampicillin and Kanamycin
7 / D / 10 III-CGTC
  Ampicillin and Kanamycin
7 / D / 11 III-CGTG
  Ampicillin and Kanamycin
7 / D / 12 III-CGTT
  Ampicillin and Kanamycin
7 / E / 1 III-CTAA
  Ampicillin and Kanamycin
7 / E / 2 III-CTAC
  Ampicillin and Kanamycin
7 / E / 3 III-CTAG
  Ampicillin and Kanamycin
7 / E / 4 III-CTAT
  Ampicillin and Kanamycin
7 / E / 5 III-CTCA
  Ampicillin and Kanamycin
7 / E / 6 III-CTCC
  Ampicillin and Kanamycin
7 / E / 7 III-CTCG
  Ampicillin and Kanamycin
7 / E / 8 III-CTCT
  Ampicillin and Kanamycin
7 / E / 9 III-CTGA
  Ampicillin and Kanamycin
7 / E / 10 III-CTGC
  Ampicillin and Kanamycin
7 / E / 11 III-CTGG
  Ampicillin and Kanamycin
7 / E / 12 III-CTGT
  Ampicillin and Kanamycin
7 / F / 1 III-CTTA
  Ampicillin and Kanamycin
7 / F / 2 III-CTTC
  Ampicillin and Kanamycin
7 / F / 3 III-CTTG
  Ampicillin and Kanamycin
7 / F / 4 III-CTTT
  Ampicillin and Kanamycin
7 / F / 5 III-GAAA
  Ampicillin and Kanamycin
7 / F / 6 III-GAAC
  Ampicillin and Kanamycin
7 / F / 7 III-GAAG
  Ampicillin and Kanamycin
7 / F / 8 III-GAAT
  Ampicillin and Kanamycin
7 / F / 9 III-GACA
  Ampicillin and Kanamycin
7 / F / 10 III-GACC
  Ampicillin and Kanamycin
7 / F / 11 III-GACG
  Ampicillin and Kanamycin
7 / F / 12 III-GACT
  Ampicillin and Kanamycin
7 / G / 1 III-GAGA
  Ampicillin and Kanamycin
7 / G / 2 III-GAGC
  Ampicillin and Kanamycin
7 / G / 3 III-GAGG
  Ampicillin and Kanamycin
7 / G / 4 III-GAGT
  Ampicillin and Kanamycin
7 / G / 5 III-GATA
  Ampicillin and Kanamycin
7 / G / 6 III-GATC
  Ampicillin and Kanamycin
7 / G / 7 III-GATG
  Ampicillin and Kanamycin
7 / G / 8 III-GATT
  Ampicillin and Kanamycin
7 / G / 9 III-GCAA
  Ampicillin and Kanamycin
7 / G / 10 III-GCAC
  Ampicillin and Kanamycin
7 / G / 11 III-GCAG
  Ampicillin and Kanamycin
7 / G / 12 III-GCAT
  Ampicillin and Kanamycin
7 / H / 1 III-GCCA
  Ampicillin and Kanamycin
7 / H / 2 III-GCCC
  Ampicillin and Kanamycin
7 / H / 3 III-GCCG
  Ampicillin and Kanamycin
7 / H / 4 III-GCCT
  Ampicillin and Kanamycin
7 / H / 5 III-GCGA
  Ampicillin and Kanamycin
7 / H / 6 III-GCGC
  Ampicillin and Kanamycin
7 / H / 7 III-GCGG
  Ampicillin and Kanamycin
7 / H / 8 III-GCGT
  Ampicillin and Kanamycin
7 / H / 9 III-GCTA
  Ampicillin and Kanamycin
7 / H / 10 III-GCTC
  Ampicillin and Kanamycin
7 / H / 11 III-GCTG
  Ampicillin and Kanamycin
7 / H / 12 III-GCTT
  Ampicillin and Kanamycin
8 / A / 1 III-GGAA
  Ampicillin and Kanamycin
8 / A / 2 III-GGAC
  Ampicillin and Kanamycin
8 / A / 3 III-GGAG
  Ampicillin and Kanamycin
8 / A / 4 III-GGAT
  Ampicillin and Kanamycin
8 / A / 5 III-GGCA
  Ampicillin and Kanamycin
8 / A / 6 III-GGCC
  Ampicillin and Kanamycin
8 / A / 7 III-GGCG
  Ampicillin and Kanamycin
8 / A / 8 III-GGCT
  Ampicillin and Kanamycin
8 / A / 9 III-GGGA
  Ampicillin and Kanamycin
8 / A / 10 III-GGGC
  Ampicillin and Kanamycin
8 / A / 11 III-GGGG
  Ampicillin and Kanamycin
8 / A / 12 III-GGGT
  Ampicillin and Kanamycin
8 / B / 1 III-GGTA
  Ampicillin and Kanamycin
8 / B / 2 III-GGTC
  Ampicillin and Kanamycin
8 / B / 3 III-GGTG
  Ampicillin and Kanamycin
8 / B / 4 III-GGTT
  Ampicillin and Kanamycin
8 / B / 5 III-GTAA
  Ampicillin and Kanamycin
8 / B / 6 III-GTAC
  Ampicillin and Kanamycin
8 / B / 7 III-GTAG
  Ampicillin and Kanamycin
8 / B / 8 III-GTAT
  Ampicillin and Kanamycin
8 / B / 9 III-GTCA
  Ampicillin and Kanamycin
8 / B / 10 III-GTCC
  Ampicillin and Kanamycin
8 / B / 11 III-GTCG
  Ampicillin and Kanamycin
8 / B / 12 III-GTCT
  Ampicillin and Kanamycin
8 / C / 1 III-GTGA
  Ampicillin and Kanamycin
8 / C / 2 III-GTGC
  Ampicillin and Kanamycin
8 / C / 3 III-GTGG
  Ampicillin and Kanamycin
8 / C / 4 III-GTGT
  Ampicillin and Kanamycin
8 / C / 5 III-GTTA
  Ampicillin and Kanamycin
8 / C / 6 III-GTTC
  Ampicillin and Kanamycin
8 / C / 7 III-GTTG
  Ampicillin and Kanamycin
8 / C / 8 III-GTTT
  Ampicillin and Kanamycin
8 / C / 9 III-TAAA
  Ampicillin and Kanamycin
8 / C / 10 III-TAAC
  Ampicillin and Kanamycin
8 / C / 11 III-TAAG
  Ampicillin and Kanamycin
8 / C / 12 III-TAAT
  Ampicillin and Kanamycin
8 / D / 1 III-TACA
  Ampicillin and Kanamycin
8 / D / 2 III-TACC
  Ampicillin and Kanamycin
8 / D / 3 III-TACG
  Ampicillin and Kanamycin
8 / D / 4 III-TACT
  Ampicillin and Kanamycin
8 / D / 5 III-TAGA
  Ampicillin and Kanamycin
8 / D / 6 III-TAGC
  Ampicillin and Kanamycin
8 / D / 7 III-TAGG
  Ampicillin and Kanamycin
8 / D / 8 III-TAGT
  Ampicillin and Kanamycin
8 / D / 9 III-TATA
  Ampicillin and Kanamycin
8 / D / 10 III-TATC
  Ampicillin and Kanamycin
8 / D / 11 III-TATG
  Ampicillin and Kanamycin
8 / D / 12 III-TATT
  Ampicillin and Kanamycin
8 / E / 1 III-TCAA
  Ampicillin and Kanamycin
8 / E / 2 III-TCAC
  Ampicillin and Kanamycin
8 / E / 3 III-TCAG
  Ampicillin and Kanamycin
8 / E / 4 III-TCAT
  Ampicillin and Kanamycin
8 / E / 5 III-TCCA
  Ampicillin and Kanamycin
8 / E / 6 III-TCCC
  Ampicillin and Kanamycin
8 / E / 7 III-TCCG
  Ampicillin and Kanamycin
8 / E / 8 III-TCCT
  Ampicillin and Kanamycin
8 / E / 9 III-TCGA
  Ampicillin and Kanamycin
8 / E / 10 III-TCGC
  Ampicillin and Kanamycin
8 / E / 11 III-TCGG
  Ampicillin and Kanamycin
8 / E / 12 III-TCGT
  Ampicillin and Kanamycin
8 / F / 1 III-TCTA
  Ampicillin and Kanamycin
8 / F / 2 III-TCTC
  Ampicillin and Kanamycin
8 / F / 3 III-TCTG
  Ampicillin and Kanamycin
8 / F / 4 III-TCTT
  Ampicillin and Kanamycin
8 / F / 5 III-TGAA
  Ampicillin and Kanamycin
8 / F / 6 III-TGAC
  Ampicillin and Kanamycin
8 / F / 7 III-TGAG
  Ampicillin and Kanamycin
8 / F / 8 III-TGAT
  Ampicillin and Kanamycin
8 / F / 9 III-TGCA
  Ampicillin and Kanamycin
8 / F / 10 III-TGCC
  Ampicillin and Kanamycin
8 / F / 11 III-TGCG
  Ampicillin and Kanamycin
8 / F / 12 III-TGCT
  Ampicillin and Kanamycin
8 / G / 1 III-TGGA
  Ampicillin and Kanamycin
8 / G / 2 III-TGGC
  Ampicillin and Kanamycin
8 / G / 3 III-TGGG
  Ampicillin and Kanamycin
8 / G / 4 III-TGGT
  Ampicillin and Kanamycin
8 / G / 5 III-TGTA
  Ampicillin and Kanamycin
8 / G / 6 III-TGTC
  Ampicillin and Kanamycin
8 / G / 7 III-TGTG
  Ampicillin and Kanamycin
8 / G / 8 III-TGTT
  Ampicillin and Kanamycin
8 / G / 9 III-TTAA
  Ampicillin and Kanamycin
8 / G / 10 III-TTAC
  Ampicillin and Kanamycin
8 / G / 11 III-TTAG
  Ampicillin and Kanamycin
8 / G / 12 III-TTAT
  Ampicillin and Kanamycin
8 / H / 1 III-TTCA
  Ampicillin and Kanamycin
8 / H / 2 III-TTCC
  Ampicillin and Kanamycin
8 / H / 3 III-TTCG
  Ampicillin and Kanamycin
8 / H / 4 III-TTCT
  Ampicillin and Kanamycin
8 / H / 5 III-TTGA
  Ampicillin and Kanamycin
8 / H / 6 III-TTGC
  Ampicillin and Kanamycin
8 / H / 7 III-TTGG
  Ampicillin and Kanamycin
8 / H / 8 III-TTGT
  Ampicillin and Kanamycin
8 / H / 9 III-TTTA
  Ampicillin and Kanamycin
8 / H / 10 III-TTTC
  Ampicillin and Kanamycin
8 / H / 11 III-TTTG
  Ampicillin and Kanamycin
8 / H / 12 III-TTTT
  Ampicillin and Kanamycin
9 / A / 1 IV-AAA
  Ampicillin and Kanamycin
9 / A / 2 IV-AAC
  Ampicillin and Kanamycin
9 / A / 3 IV-AAG
  Ampicillin and Kanamycin
9 / A / 4 IV-AAT
  Ampicillin and Kanamycin
9 / A / 5 IV-ACA
  Ampicillin and Kanamycin
9 / A / 6 IV-ACC
  Ampicillin and Kanamycin
9 / A / 7 IV-ACG
  Ampicillin and Kanamycin
9 / A / 8 IV-ACT
  Ampicillin and Kanamycin
9 / A / 9 IV-AGA
  Ampicillin and Kanamycin
9 / A / 10 IV-AGC
  Ampicillin and Kanamycin
9 / A / 11 IV-AGG
  Ampicillin and Kanamycin
9 / A / 12 IV-AGT
  Ampicillin and Kanamycin
9 / B / 1 IV-ATA
  Ampicillin and Kanamycin
9 / B / 2 IV-ATC
  Ampicillin and Kanamycin
9 / B / 3 IV-ATG
  Ampicillin and Kanamycin
9 / B / 4 IV-ATT
  Ampicillin and Kanamycin
9 / B / 5 IV-CAA
  Ampicillin and Kanamycin
9 / B / 6 IV-CAC
  Ampicillin and Kanamycin
9 / B / 7 IV-CAG
  Ampicillin and Kanamycin
9 / B / 8 IV-CAT
  Ampicillin and Kanamycin
9 / B / 9 IV-CCA
  Ampicillin and Kanamycin
9 / B / 10 IV-CCC
  Ampicillin and Kanamycin
9 / B / 11 IV-CCG
  Ampicillin and Kanamycin
9 / B / 12 IV-CCT
  Ampicillin and Kanamycin
9 / C / 1 IV-CGA
  Ampicillin and Kanamycin
9 / C / 2 IV-CGC
  Ampicillin and Kanamycin
9 / C / 3 IV-CGG
  Ampicillin and Kanamycin
9 / C / 4 IV-CGT
  Ampicillin and Kanamycin
9 / C / 5 IV-CTA
  Ampicillin and Kanamycin
9 / C / 6 IV-CTC
  Ampicillin and Kanamycin
9 / C / 7 IV-CTG
  Ampicillin and Kanamycin
9 / C / 8 IV-CTT
  Ampicillin and Kanamycin
9 / C / 9 IV-GAA
  Ampicillin and Kanamycin
9 / C / 10 IV-GAC
  Ampicillin and Kanamycin
9 / C / 11 IV-GAG
  Ampicillin and Kanamycin
9 / C / 12 IV-GAT
  Ampicillin and Kanamycin
9 / D / 1 IV-GCA
  Ampicillin and Kanamycin
9 / D / 2 IV-GCC
  Ampicillin and Kanamycin
9 / D / 3 IV-GCG
  Ampicillin and Kanamycin
9 / D / 4 IV-GCT
  Ampicillin and Kanamycin
9 / D / 5 IV-GGA
  Ampicillin and Kanamycin
9 / D / 6 IV-GGC
  Ampicillin and Kanamycin
9 / D / 7 IV-GGG
  Ampicillin and Kanamycin
9 / D / 8 IV-GGT
  Ampicillin and Kanamycin
9 / D / 9 IV-GTA
  Ampicillin and Kanamycin
9 / D / 10 IV-GTC
  Ampicillin and Kanamycin
9 / D / 11 IV-GTG
  Ampicillin and Kanamycin
9 / D / 12 IV-GTT
  Ampicillin and Kanamycin
9 / E / 1 IV-TAA
  Ampicillin and Kanamycin
9 / E / 2 IV-TAC
  Ampicillin and Kanamycin
9 / E / 3 IV-TAG
  Ampicillin and Kanamycin
9 / E / 4 IV-TAT
  Ampicillin and Kanamycin
9 / E / 5 IV-TCA
  Ampicillin and Kanamycin
9 / E / 6 IV-TCC
  Ampicillin and Kanamycin
9 / E / 7 IV-TCG
  Ampicillin and Kanamycin
9 / E / 8 IV-TCT
  Ampicillin and Kanamycin
9 / E / 9 IV-TGA
  Ampicillin and Kanamycin
9 / E / 10 IV-TGC
  Ampicillin and Kanamycin
9 / E / 11 IV-TGG
  Ampicillin and Kanamycin
9 / E / 12 IV-TGT
  Ampicillin and Kanamycin
9 / F / 1 IV-TTA
  Ampicillin and Kanamycin
9 / F / 2 IV-TTC
  Ampicillin and Kanamycin
9 / F / 3 IV-TTG
  Ampicillin and Kanamycin
9 / F / 4 IV-TTT
  Ampicillin and Kanamycin
9 / F / 5 pTAL_GFP
  Ampicillin
9 / F / 6 pTAL_RFP
  Ampicillin
Data from: CACHED @ 2014-12-21 12:42:55.467945