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Thomas Wandless Lab Plasmids


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Title Authors Publication
Dicistronic regulation of fluorescent proteins in the budding yeast Saccharomyces cerevisiae. Edwards SR, Wandless TJ Yeast. 2009 Dec 17. ():.
A general chemical method to regulate protein stability in the mammalian central nervous system. Iwamoto M, Bjoerklund T, Lundberg C, Kirik D, Wandless TJ Chem Biol. 2010 Sep 24. 17(9):981-8.
A rapid, reversible, and tunable method to regulate protein function in living cells using synthetic small molecules. Banaszynski LA, Chen LC, Maynard-Smith LA, Ooi AG, Wandless TJ Cell. 2006 Sep 8;126(5):995-1004.
Recent progress with FKBP-derived destabilizing domains. Chu BW, Banaszynski LA, Chen LC, Wandless TJ Bioorg Med Chem Lett. 2008 Nov 15;18(22):5941-4. Epub 2008 Sep 12.
Small-molecule displacement of a cryptic degron causes conditional protein degradation. Bonger KM, Chen LC, Liu CW, Wandless TJ Nat Chem Biol. 2011 Jul 3. doi: 10.1038/nchembio.598.
Destabilizing domains derived from the human estrogen receptor. Miyazaki Y, Imoto H, Chen LC, Wandless TJ J Am Chem Soc. 2012 Mar 7;134(9):3942-5. Epub 2012 Feb 22.
Rapid and Tunable Control of Protein Stability in Caenorhabditis elegans Using a Small Molecule. Cho U, Zimmerman SM, Chen LC, Owen E, Kim JV, Kim SK, Wandless TJ PLoS One. 2013 Aug 22;8(8):e72393. doi: 10.1371/journal.pone.0072393.
A General Method for Regulating Protein Stability with Light. Bonger KM, Rakhit R, Payumo AY, Chen JK, Wandless TJ ACS Chem Biol. 2013 Nov 1.
Wandless lab ecDHFR plasmid (unpublished)
Ligand-switchable substrates for a ubiquitin-proteasome system. Egeler EL, Urner LM, Rakhit R, Liu CW, Wandless TJ J Biol Chem. 2011 Sep 9;286(36):31328-36. doi: 10.1074/jbc.M111.264101. Epub 2011 Jul 15.
A method to rapidly create protein aggregates in living cells. Miyazaki Y, Mizumoto K, Dey G, Kudo T, Perrino J, Chen LC, Meyer T, Wandless TJ Nat Commun. 2016 May 27;7:11689. doi: 10.1038/ncomms11689.
A Novel Destabilizing Domain Based on a Small-Molecule Dependent Fluorophore. Navarro R, Chen LC, Rakhit R, Wandless TJ ACS Chem Biol. 2016 Jun 6.
ID Plasmid Gene/Insert Vector Type Publication Hidden Extra Search Info  
24378pGADT7-ADH700-yeCherry-p150-yeGFP-DHFRpADH1 yeCherry::p150yeGFP-DHFR (Saccharomyces cerevisiae)Yeast ExpressionDicistronic regulation of fluorescent proteins in the budding yeast Saccharomyces cerevisiae. Yeast. 2009 Dec 17. ():. pGADT7 Add to Cart
24549pGADT7-ADH700-yeCherry-YAP1-yeGFP-DHFRpADH1 yeCherry::YAP1 yeGFP-DHFR (Saccharomyces cerevisiae)Yeast ExpressionDicistronic regulation of fluorescent proteins in the budding yeast Saccharomyces cerevisiae. Yeast. 2009 Dec 17. ():. pGADT7 YAP1 YML007W, PAR1, PDR4, SNQ3 Add to Cart
24584pGADT7-ADH700-yeCherry-pTEF1 (S.C.)-yeGFP-DHFRpADH1 yeCherry::pTEF1 (S.C.)yeGFP-DHFR (Saccharomyces cerevisiae)Yeast ExpressionDicistronic regulation of fluorescent proteins in the budding yeast Saccharomyces cerevisiae. Yeast. 2009 Dec 17. ():. pGADT7 Add to Cart
24585pGADT7-ADH700-yeCherry-pTEF1(ashbya)-yeGFP-DHFRpADH1 yeCherry::pTEF1 (Ashbya)yeGFP-DHFR (Saccharomyces cerevisiae)Yeast ExpressionDicistronic regulation of fluorescent proteins in the budding yeast Saccharomyces cerevisiae. Yeast. 2009 Dec 17. ():. pGADT7 Add to Cart
29325pBMN DHFR(DD)-YFPDHFR(DD)-YFP (Other)RetroviralA general chemical method to regulate protein stability in the mammalian central nervous system. Chem Biol. 2010 Sep 24. 17(9):981-8. pBMN Add to Cart
29326pBMN YFP-DHFR(DD)YFP-DHFR(DD) (Other)RetroviralA general chemical method to regulate protein stability in the mammalian central nervous system. Chem Biol. 2010 Sep 24. 17(9):981-8. pBMN Add to Cart
31763pBMN FKBP(DD)-YFPFKBP(DD)-YFP-HA (Homo sapiens)RetroviralA rapid, reversible, and tunable method to regulate protein function in living cells using synthetic small molecules. Cell. 2006 Sep 8;126(5):995-1004. pBMN Add to Cart
31766pBMN YFP-FKBP(DD)HA-YFP-FKBP(DD) (Homo sapiens)RetroviralRecent progress with FKBP-derived destabilizing domains. Bioorg Med Chem Lett. 2008 Nov 15;18(22):5941-4. Epub 2008 Sep 12. pBMN Add to Cart
31767pBMN YFP-LIDYFP-LID (Homo sapiens)RetroviralSmall-molecule displacement of a cryptic degron causes conditional protein degradation. Nat Chem Biol. 2011 Jul 3. doi: 10.1038/nchembio.598. pBMN Add to Cart
37259pBMN ER50-YFPER50 (Homo sapiens)RetroviralDestabilizing domains derived from the human estrogen receptor. J Am Chem Soc. 2012 Mar 7;134(9):3942-5. Epub 2012 Feb 22. pBMN iHcRed ESR1 ER, ESR, ESRA, ESTRR, Era, NR3A1 Add to Cart
37261PBMN YFP-ER50ER50 (Homo sapiens)RetroviralDestabilizing domains derived from the human estrogen receptor. J Am Chem Soc. 2012 Mar 7;134(9):3942-5. Epub 2012 Feb 22. pBMN iHcRed ESR1 ER, ESR, ESRA, ESTRR, Era, NR3A1 Add to Cart
47076pBMN ltDHFR(DD)-YFPltDHFR(DD) (Other)RetroviralRapid and Tunable Control of Protein Stability in Caenorhabditis elegans Using a Small Molecule. PLoS One. 2013 Aug 22;8(8):e72393. doi: 10.1371/journal.pone.0072393. ecDHFR based destable domain function at low temperature for regulating protein stability in Caenorhabditis elegans pBMN Add to Cart
47080pBMN YFP-ltDHFR(DD)YFP-ltDHFR(DD) (Other)RetroviralRapid and Tunable Control of Protein Stability in Caenorhabditis elegans Using a Small Molecule. PLoS One. 2013 Aug 22;8(8):e72393. doi: 10.1371/journal.pone.0072393. ecDHFR based destable domain function at low temperature for regulating protein stability in Caenorhabditis elegans pBMN Add to Cart
49570pBMN HAYFP-LOV24YFP-LOV24 (Other)RetroviralA General Method for Regulating Protein Stability with Light. ACS Chem Biol. 2013 Nov 1. regulate protein stability with light pBMN Add to Cart
73180pET15b His6-FKBPF36VFKBP(F36V) (Homo sapiens)Bacterial ExpressionLigand-switchable substrates for a ubiquitin-proteasome system. J Biol Chem. 2011 Sep 9;286(36):31328-36. doi: 10.1074/jbc.M111.264101. Epub 2011 Jul 15. Expressing FKBPF36V protein in washing out shield-1 ligand for FKBPdd pET15b FKBP1A FKBP-12, FKBP-1A, FKBP1, FKBP12, PKC12, PKCI2, PPIASE Add to Cart
73188pET15b His6-ecDHFR (WT)folA dihydrofolate reductase (Other)Bacterial Expression Wandless lab ecDHFR plasmid (unpublished) bacterial express ecDHFR protein for washing out TMP ligand for pBMN DHFR(DD)-YFP (Plasmid #29325) and pBMN YFP-DHFR(DD) (Plasmid #29326) pET15b folA b0048, ECK0049, JW0047, tmrA Add to Cart
78289AgDDNES-L106P-sfGFP (Homo sapiens)Mammalian ExpressionA method to rapidly create protein aggregates in living cells. Nat Commun. 2016 May 27;7:11689. doi: 10.1038/ncomms11689. create protein aggregates in living cells PiggyBac transformation vector pB FKBP1A FKBP-12, FKBP-1A, FKBP1, FKBP12, PKC12, PKCI2, PPIASE Add to Cart
80625NLS-AgDDNLS-NES-L106P-sfGFP (Homo sapiens)Mammalian ExpressionA method to rapidly create protein aggregates in living cells. Nat Commun. 2016 May 27;7:11689. doi: 10.1038/ncomms11689. create nuclear protein aggregates in mammalian cells PiggyBac transformation vector pB Add to Cart
80626Pvha6-AgDDNES-L106P-sfGFP (Homo sapiens)Worm ExpressionA method to rapidly create protein aggregates in living cells. Nat Commun. 2016 May 27;7:11689. doi: 10.1038/ncomms11689. create protein aggregates in C. elegans modified worm expression vector with Pvha6 promoter Add to Cart
80629mCherry-FDDUnaG (Other)RetroviralA Novel Destabilizing Domain Based on a Small-Molecule Dependent Fluorophore. ACS Chem Biol. 2016 Jun 6. Fluorescent destablizing domain for regulating protein stability and fluorescence pBMN Add to Cart