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Yunde Zhao Lab Plasmids


The Yunde Zhao Lab has deposited plasmids at Addgene for distribution to the research community. Addgene is a nonprofit plasmid repository dedicated to improving life science research.

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Title Authors Publication
Self-processing of ribozyme-flanked RNAs into guide RNAs in vitro and in vivo for CRISPR-mediated genome editing. Gao Y, Zhao Y J Integr Plant Biol. 2013 Dec 30. doi: 10.1111/jipb.12152.
An effective strategy for reliably isolating heritable and Cas9-free Arabidopsis mutants generated by CRISPR/Cas9-mediated genome editing. Gao X, Chen J, Dai X, Zhang D, Zhao Y Plant Physiol. 2016 May 15. pii: pp.00663.2016.
ID Plasmid Gene/Insert Vector Type Publication Hidden Extra Search Info  
51055pACT2-CAS9cCas9c (Synthetic)Bacterial Expression, Yeast Expression, CRISPRSelf-processing of ribozyme-flanked RNAs into guide RNAs in vitro and in vivo for CRISPR-mediated genome editing. J Integr Plant Biol. 2013 Dec 30. doi: 10.1111/jipb.12152. Express Eukaryotic-codon-optimized Cas9c gene in yeast under ADH1 promoter for genome editing. SV40 NLS is fused to the C terminal. GAL4 region from pACT2 is removed. pACT2 Add to Cart
51056pRS316-RGR-GFPRGR-GFP (Synthetic)Bacterial Expression, Yeast Expression, CRISPRSelf-processing of ribozyme-flanked RNAs into guide RNAs in vitro and in vivo for CRISPR-mediated genome editing. J Integr Plant Biol. 2013 Dec 30. doi: 10.1111/jipb.12152. Express self-cleaving-ribozyme-flanked sgRNA cassette (RGR) targeting GFP for CRISPR systems in yeast driven by ADH1 promoter. RGR has a HH ribozyme at its 5', and an HDV ribozyme at its 3'. pRS316 Add to Cart
51057pRS316-RGR-GFP-mHHRGR-GFP (Synthetic)Bacterial Expression, Yeast Expression, CRISPRSelf-processing of ribozyme-flanked RNAs into guide RNAs in vitro and in vivo for CRISPR-mediated genome editing. J Integr Plant Biol. 2013 Dec 30. doi: 10.1111/jipb.12152. Same as pRS316-RGR-GFP except for the HH ribozyme region which has a 13 bp deletion at its 5' end. pRS316 Add to Cart
51058pRS316-RGR-GFP-mHDVRGR-GFP (Synthetic)Bacterial Expression, Yeast Expression, CRISPRSelf-processing of ribozyme-flanked RNAs into guide RNAs in vitro and in vivo for CRISPR-mediated genome editing. J Integr Plant Biol. 2013 Dec 30. doi: 10.1111/jipb.12152. Same as pRS316-RGR-GFP except for the HDV ribozyme region which has a 15 bp deletion at its 3' end. pRS316 Add to Cart
51059pRS316-RGR-GFP-mmRGR-GFP (Synthetic)Bacterial Expression, Yeast Expression, CRISPRSelf-processing of ribozyme-flanked RNAs into guide RNAs in vitro and in vivo for CRISPR-mediated genome editing. J Integr Plant Biol. 2013 Dec 30. doi: 10.1111/jipb.12152. Same as pRS316-RGR-GFP except for the HH ribozyme region which has a 13 bp deletion at its 5' end, and for the HDV ribozyme region which has a 15 bp deletion at its 3' end. pRS316 Add to Cart
78931pHDE-35S-Cas9-mCherryPlant Expression, CRISPRAn effective strategy for reliably isolating heritable and Cas9-free Arabidopsis mutants generated by CRISPR/Cas9-mediated genome editing. Plant Physiol. 2016 May 15. pii: pp.00663.2016. For CRISPR/Cas9 mediated gene editing in Arabidopsis; provides a visual screen for Cas9-free plants pHDE Add to Cart
78932pHDE-35S-Cas9-mCherry-UBQPlant Expression, CRISPRAn effective strategy for reliably isolating heritable and Cas9-free Arabidopsis mutants generated by CRISPR/Cas9-mediated genome editing. Plant Physiol. 2016 May 15. pii: pp.00663.2016. For CRISPR/Cas9 mediated gene editing in Arabidopsis; provides a visual screen for Cas9-free plants. Enable production of two gRNAs pHDE Add to Cart