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Intron targeting-mediated and endogenous gene integrity-maintaining knockin in zebrafish using the CRISPR/Cas9 system.
Li J, Zhang BB, Ren YG, Gu SY, Xiang YH, Huang C, Du JL
Cell Res. 2015 Apr 7. doi: 10.1038/cr.2015.43.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
65562th-P2A-EGFP donorIntron targeting-mediated EGFP knockin at the zebrafish th locus.
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65563th-P2A-Gal4 donorIntron targeting-mediated Gal4 knockin at the zebrafish th locus.
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65564gfap-EGFP donorIntron targeting-mediated EGFP knockin at the zebrafish gfap locus
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65565pT7-th-sgRNAin vitro trancription of sgRNA targeting the zebrafish th locus
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65566pT7-gfap-sgRNAin vitro trancription of sgRNA targeting the zebrafish gfap locus
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