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Electron donation to an archaeal cytochrome P450 is enhanced by PCNA-mediated selective complex formation with foreign redox proteins.
Suzuki R, Hirakawa H, Nagamune T
Biotechnol J. 2014 Dec;9(12):1573-81. doi: 10.1002/biot.201400007. Epub 2014 Jul 21. PubMed Article

Plasmids from Article

ID Plasmid Purpose
66131pET11a+CYP119Expresses the wild type of CYP119
66134pET11a+T214VExpresses the T214V mutant of CYP119
66135pET11a+D77R/T214VExpresses the D77R/T214V mutant of CYP119
66136pET11+PCNA3-CYP119Expresses the wild type of CYP119 fused to Sulfolobus solfataricus PCNA3
66137pET11+PCNA3-CYP119(T214V)Expresses the T214V mutant of CYP119 fused to Sulfolobus solfataricus PCNA3
66138pET11+PCNA3-CYP119(D77R/T214V)Expresses the D77R/T214V mutant of CYP119 fused to Sulfolobus solfataricus PCNA3

Antibodies from Article