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Addgene
Introduction of selective intersubunit disulfide bonds into self-assembly protein scaffold to enhance an artificial multienzyme complex's activity.
Hirakawa H, Kakitani A, Nagamune T
Biotechnol Bioeng. 2013 Jul;110(7):1858-64. doi: 10.1002/bit.24861. Epub 2013 Feb 22. PubMed Article

Plasmids from Article

ID Plasmid Purpose
66139pET15b+PCNA1 WTExpresses the wild type of Sulfolobus solfataricus PCNA1
66140pET15b+PCNA1 G108CExpresses the G108C mutant of Sulfolobus solfataricus PCNA1
66173pET15b+PCNA1 P186CExpresses the P186C mutant of Sulfolobus solfataricus PCNA1
66174pET15b+PCNA1 G108C/P186CExpresses the G108C/P186C mutant of Sulfolobus solfataricus PCNA1
66175pET15b+PCNA2 WTExpresses the wild type of Sulfolobus solfataricus PCNA2
66176pET15b+PCNA2 E105CExpresses the E105C mutant of Sulfolobus solfataricus PCNA2
66177pET15b+PCNA2 L171CExpresses the L171C mutant of Sulfolobus solfataricus PCNA2
66178pET15b+PCNA2 E105C/L171CExpresses the E105C/L171C mutant of Sulfolobus solfataricus PCNA2
66179pET15b+PCNA3 WTExpresses the wild type of Sulfolobus solfataricus PCNA3
66180pET15b+PCNA3 R112CExpresses the R112C mutant of Sulfolobus solfataricus PCNA3
66181pET15b+PCNA3 T180CExpresses the T180C mutant of Sulfolobus solfataricus PCNA3
66182pET15b+PCNA3 R112C/T180CExpresses the R112C/T180C mutant of Sulfolobus solfataricus PCNA3

Antibodies from Article