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YeastFab: the design and construction of standard biological parts for metabolic engineering in Saccharomyces cerevisiae.
Guo Y, Dong J, Zhou T, Auxillos J, Li T, Zhang W, Wang L, Shen Y, Luo Y, Zheng Y, Lin J, Chen GQ, Wu Q, Cai Y, Dai J
Nucleic Acids Res. 2015 May 8. pii: gkv464.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
65314HcKan_Preceiving vector of promoters for the construction of standard promoter parts in YeastFab work
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65315HcKan_Oreceiving vector of ORFs for the construction of standard ORF parts in YeastFab work
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65316HcKan_Treceiving vector of terminators for the construction of standard terminator parts in YeastFab work
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65318POT2-RFPreceiving vector of standard transcription units(TUs) in YeastFab work, useful for further assembly
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65319POT3-RFPreceiving vector of standard transcription units(TUs) in YeastFab work, useful for further assembly
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65320POT4-RFPreceiving vector of standard transcription units(TUs) in YeastFab work, useful for further assembly
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65321POT5-RFPreceiving vector of standard transcription units(TUs) in YeastFab work, useful for further assembly
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65322POT6-RFPreceiving vector of standard transcription units(TUs) in YeastFab work, useful for further assembly
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65323POT7-RFPreceiving vector of standard transcription units(TUs) in YeastFab work, useful for further assembly
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65324POT8-RFPreceiving vector of standard transcription units(TUs) in YeastFab work, useful for further assembly
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65325POT9-RFPreceiving vector of standard transcription units(TUs) in YeastFab work, useful for further assembly
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65326POT10-RFPreceiving vector of standard transcription units(TUs) in YeastFab work, useful for further assembly
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65327POT11-RFPreceiving vector of standard transcription units(TUs) in YeastFab work, useful for further assembly
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65328pYG026reporter plasmids used in YeastFab work, in which promoters could be inserted just upstream YFP(YEVenus) to drive its expression, also contains an mCherry gene driven by TEF2 promoter.
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65329pRS414-URRCEN plasmid as receiving vector for exogenous pathway
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65330pRS424-URRhigh copy number plasmid as receiving vector for exogenous pathway
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65331pMV-URR1standard plasmids for exogenus pathway assembly haboring recombination sequence 1(Universal Recombination Region 1) which could be released and ligated to series of TUs
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65332pMV-URR2standard plasmids for exogenus pathway assembly haboring recombination sequence 2 (Universal Recombination Region 2) which could be released and ligated to series of TUs
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65333pMV-HIS3standard plasmids for exogenus pathway assembly haboring yeast auxotrophic marker HIS3 which could be released and ligated to series of TUs
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65334pMV-LEU2standard plasmids for exogenus pathway assembly haboring yeast auxotrophic marker LEU2 which could be released and ligated to series of TUs
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65335pMV-TRP1standard plasmids for exogenus pathway assembly haboring yeast auxotrophic marker TRP1 which could be released and ligated to series of TUs
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65336HCKan-P-TDH3construction of TDH3 promoter in HCKan-P receiving vector
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65337HCKan-P-TEF2construction of TEF2 promoter in HCKan-P receiving vector
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65338HCKan-P-CYC1construction of CYC1 promoter in HCKan-P receiving vector
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65339HCKan-T-ADH1construction of ADH1 terminator in HCKan-P receiving vector
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65340HCKan-T-TEF2construction of TEF2 terminator in HCKan-P receiving vector
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65341PMV-ycrtEsysthesis of crtE gene in the beta-carotene pathway
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65342PMV-ycrtIsysthesis of crtI gene in the beta-carotene pathway
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65343PMV-ycrtYBsysthesis of crtYB gene in the beta-carotene pathway
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65344HCKan-O-ycrtEconstruction of ycrtE gene in HCKan-O receiving vector
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65345HCKan-O-ycrtIconstruction of ycrtI gene in HCKan-O receiving vector
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65346HCKan-O-ycrtYBconstruction of ycrtYB gene in HCKan-O receiving vector
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65347pKMV-VioAsysthesis of vioA gene in the violacein pathway
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65348pKMV-VioBsysthesis of vioB gene in the violacein pathway
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65349pKMV-VioCsysthesis of vioC gene in the violacein pathway
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65350pKMV-VioDsysthesis of vioD gene in the violacein pathway
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65351pKMV-VioEsysthesis of vioE gene in the violacein pathway
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65352POT2-TDH3p-ycrtE-ADH1tTU for combinatorial construction of ?-carotene pathway
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65353POT2-TEF2p-ycrtE-ADH1tTU for combinatorial construction of ?-carotene pathway
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65354POT2-CYC1p-ycrtE-ADH1tTU for combinatorial construction of ?-carotene pathway
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65355POT4-TDH3p-ycrtI-ADH1tTU for combinatorial construction of ?-carotene pathway
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65356POT4-TEF2p-ycrtI-ADH1tTU for combinatorial construction of ?-carotene pathway
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65357POT4-CYC1p-ycrtI-ADH1tTU for combinatorial construction of ?-carotene pathway
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65358POT5-TDH3p-ycrtYB-ADH1tTU for combinatorial construction of ?-carotene pathway
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65359POT5-TEF2p-ycrtYB-ADH1tTU for combinatorial construction of ?-carotene pathway
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65360POT5-CYC1p-ycrtYB-ADH1tTU for combinatorial construction of ?-carotene pathway
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