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Cell-free protein synthesis from a release factor 1 deficient Escherichia coli activates efficient and multiple site-specific nonstandard amino acid incorporation.
Hong SH, Ntai I, Haimovich AD, Kelleher NL, Isaacs FJ, Jewett MC
ACS Synth Biol. 2014 Jun 20;3(6):398-409. doi: 10.1021/sb400140t. Epub 2014 Jan 2.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
69494rEc.13Recoded strain from MG1655 with prfA
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69495rEc.13.delARecoded strain from MG1655, prfA deleted
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