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Engineering a Circular Riboregulator in Escherichia coli.
Rostain W, Shen S, Cordero T, Rodrigo G, Jaramillo A
Biodes Res. 2020 Sep 12;2020:1916789. doi: 10.34133/2020/1916789. eCollection 2020. PubMed Article

Plasmids from Article

ID Plasmid Purpose
69926circRAJ31Expresses RAJ31 riboregulator encased in a permuted intron exon ribozyme, which circularises the riboregulator.
69928cisRAJ31-GFP-LAAExpresses cisRAJ31-GFP-LAA, a cis-repressed mRNA regulated by RAJ31.
69930circRAJ31-Toehold_mutantExpresses a toehold mutant of the circRAJ31 riboregulator encased in a permuted intron exon ribozyme, which circularises the riboregulator. This mutant does not activate cisRAJ31.
69931pT7_circRAJ31Expresses RAJ31 riboregulator encased in a permuted intron exon ribozyme, which circularises the riboregulator. Under control of a T7 promoter.
69932pT7_circRAJ31_C873UExpresses RAJ31 riboregulator encased in a permuted intron exon ribozyme, which circularises the riboregulator. This mutant is deficient in circularisation. Under control of a T7 promoter.
69933cisRAJ31-catExpresses cisRAJ31-cat, a cis-repressed mRNA regulated by RAJ31.
69934taRAJ31minExpresses taRAJ31min, a linear riboregulator.

Antibodies from Article