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Rapid cell-free forward engineering of novel genetic ring oscillators.
Niederholtmeyer H, Sun ZZ, Hori Y, Yeung E, Verpoorte A, Murray RM, Maerkl SJ
Elife. 2015 Oct 2;4. doi: 10.7554/eLife.09771.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
70716pET21a(+)-Histag-Cerulean (526) Expression vector for Cerulean purification
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70718pET21a(+)-Histag-Citrine (525)Expression vector for Citrine purification
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70719pET21a(+)-Histag-mCherry (527)Expression vector for mCherry purification
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70723pBetI-BCD7-Citrine P69U18C68T11 v40-1 (532)Citrine reporter for transfer fxns
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70725pLacI-BCD7-Citrine P42U18C68T11 v40-1 (536)Citrine reporter for transfer fxns
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70726pCI-BCD7-Citrine P62U18C68T11 v40-1 (523)Citrine reporter for transfer fxns
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707273n1 (572)BetI/PhlF/SrpR BCD7 3-node v41-1
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707305n1 (560)TetR/PhlF/SrpR/LambdaCI/LacI BCD7 5-node v41-2
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70731pBetI-BCD7-phlF-ssrA P69U18C51T15 v40-3 (570)plasmid for building 3n1
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70732pBetI-BCD7-qacR-ssrA P69U18C63T16 v50-2 (568)plasmid for building 5n2
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70733pLacO1-BCD7-tetR-ssrA P42U18C45T13 v40-5a (497)plasmid for building 5n1
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70734pSrpR-BCD7-betI-ssrA P65U18C64T14 v50-2 (567)plasmid for building 3n1, 5n2
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70735pSrpR-BCD7-lambdaCI-ssrA P65U18C47T14 v40-2 (495)plasmid for building 5n1
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70736pSrpR-BCD7-tetR-ssrA P65U18C45T14 v40-2 (491)plasmid for building 3n2
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70737pCI-BCD7-lacI-ssrA P62U18C50T16 v40-4a (496)plasmid for building 5n1
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70739pQacR-BCD7-tetR-ssrA P68U18C45T13 v40-5 (569)plasmid for building 5n2
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70740pTetR-BCD7-phlF-ssrA P66U18C51T15 v40-3a (492)plasmid for building 3n2, 5n1, 5n2
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70741pPhlF-BCD20-sfGFP-ssrA P64U23C39T13 v1-1 (462)1 color strong reporter used in study
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70742pPhlF-BCD22-sfGFP-ssrA P64U26C39T11 v1-1 (632)1 color weak reporter used in study
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70743pPhlF-BCD20-Citrine-ssrA P64U23C88T18 v1-1 (586)for building 3-color reporter plasmid
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70744pSrpR-BCD20-Cerulean-ssrA P65U23C89T19 v50-2a (587)for building 3-color reporter plasmid
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70745pTetR-BCD20-mCherry-ssrA P66U23C90T20 v40-3a (588)for building 3-color reporter plasmid
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