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DNA-binding-domain fusions enhance the targeting range and precision of Cas9.
Bolukbasi MF, Gupta A, Oikemus S, Derr AG, Garber M, Brodsky MH, Zhu LJ, Wolfe SA
Nat Methods. 2015 Oct 19. doi: 10.1038/nmeth.3624.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
69219pCSDest2-SpCas9-NLS-3XHA-NLS-Zif268Expresses wild type SpCas9 fused to Zif268 in mammalian cells
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69220pCSDest2-SpCas9-NLS-3XHA-NLSExpresses wild type SpCas9 in mammalian cells
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69221pCSDest2-SpCas9-MT1-NLS-3XHA-NLSExpresses R1333K mutant SpCas9 in mammalian cell
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69222pCSDest2-SpCas9-MT2-NLS-3XHA-NLSExpresses R1333S mutant SpCas9 in mammalian cell
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69223pCSDest2-SpCas9-MT3-NLS-3XHA-NLSExpresses R1335K mutant SpCas9 in mammalian cell
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69224pCSDest2-SpCas9-MT3-NLS-3XHA-NLS-Zif268Expresses R1335K mutant SpCas9 fused to Zif268 in mammalian cell
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69225pCSDest2-SpCas9-WT-NLS-3XHA-NLS-ZFP_TS2Expresses wild type SpCas9 fused to ZFP-TS2 in mammalian cells
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69226pCSDest2-SpCas9-WT-NLS-3XHA-NLS-ZFP_TS3Expresses wild type SpCas9 fused to ZFP-TS3 in mammalian cells
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69227pCSDest2-SpCas9-WT-NLS-3XHA-NLS-ZFP_TS4Expresses wild type SpCas9 fused to ZFP-TS4 in mammalian cells
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69228pCSDest2-SpCas9-MT3-NLS-3XHA-NLS-ZFP_TS2Expresses R1335K mutant SpCas9 fused to ZFP-TS2 in mammalian cell
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69229pCSDest2-SpCas9-MT3-NLS-3XHA-NLS-ZFP_TS3Expresses R1335K mutant SpCas9 fused to ZFP-TS3 in mammalian cell
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69230pCSDest2-SpCas9-MT3-NLS-3XHA-NLS-ZFP_TS4Expresses R1335K mutant SpCas9 fused to ZFP-TS4 in mammalian cell
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69231pCSDest2-2XNLS-SpCas9-MT3-NLS-3XHA-NLS-TALE_TS3Expresses R1335K mutant SpCas9 fused to TALE-TS3 in mammalian cell
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69232pCSDest2-2XNLS-SpCas9-WT-NLS-3XHA-NLS-TALentryWild type SpCas9 expression plasmid to clone TALEs through BbsI site (generates compatible overhangs with Acc65I and BamHI sites)
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69233pCSDest2-2XNLS-SpCas9-MT3-NLS-3XHA-NLS-TALentryR1335K mutant SpCas9 expression plasmid to clone TALEs through BbsI site (generates compatible overhangs with Acc65I and BamHI sites)
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69234pLKO1-puro-U6-sgRNA-TS2U6 driven SpCas9 sgRNA expression for TS2 site
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69235pLKO1-puro-U6-sgRNA-TS3U6 driven SpCas9 sgRNA expression for TS3 site
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69236pLKO1-puro-U6-sgRNA-TS4U6 driven SpCas9 sgRNA expression for TS4 site
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69237pLKO1-puro-U6-sgRNA-LRTM2U6 driven SpCas9 sgRNA expression for LRTM2 site
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69238pLKO1-puro-U6-sgRNA-KANK3U6 driven SpCas9 sgRNA expression for KANK3 site
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69239pLKO1-puro-U6-sgRNA-TGM2U6 driven SpCas9 sgRNA expression for TGM2 site
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69240pLKO1-puro-U6-sgRNA-PLXNB2U6 driven SpCas9 sgRNA expression for PLXNB2 site
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