Egg cell-specific promoter-controlled CRISPR/Cas9 efficiently generates homozygous mutants for multiple target genes in Arabidopsis in a single generation.
Wang ZP, Xing HL, Dong L, Zhang HY, Han CY, Wang XC, Chen QJ
Genome Biol. 2015 Jul 21;16:144. doi: 10.1186/s13059-015-0715-0. PubMed
Wang ZP, Xing HL, Dong L, Zhang HY, Han CY, Wang XC, Chen QJ
Genome Biol. 2015 Jul 21;16:144. doi: 10.1186/s13059-015-0715-0. PubMed
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 71286 | pHEE401 | Egg cell-specific promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 promoter), Hyg resistance |
| 71287 | pHEE401E | Egg cell-specific promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 promoter), Hyg resistance |
| 71288 | pHEE2E-TRI | Egg cell-specific promoter-controlled expression 3×FLAG-NLS-zCas9-NLS and two sgRNAs targeting three genes: ETC2, TRY, and CPC |
| 91715 | pKEE401 | CRISPR/Cas9-mediated genome editing in Arabidopsis. Contains Cas9 and empty gRNA scaffold, Kan resistance |
| 91716 | pXEE401B | CRISPR/Cas9-mediated genome editing in Arabidopsis. Contains Cas9 and empty gRNA scaffold, Bar resistance |
| 91717 | pXEE401BG | CRISPR/Cas9-mediated genome editing in Arabidopsis, Bar and glyphosate-resistance |