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Highly specific epigenome editing by CRISPR-Cas9 repressors for silencing of distal regulatory elements.
Thakore PI, D'Ippolito AM, Song L, Safi A, Shivakumar NK, Kabadi AM, Reddy TE, Crawford GE, Gersbach CA
Nat Methods. 2015 Dec;12(12):1143-9. doi: 10.1038/nmeth.3630. Epub 2015 Oct 26.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
71236pLV hU6-sgRNA hUbC-dCas9-KRAB-T2a-PuroExpress sgRNA and dCas9-KRAB from lentiviral vector
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71237pLV hU6-sgRNA hUbC-dCas9-KRAB-T2a-GFPExpress sgRNA and dCas9-KRAB from 3rd generation lentiviral vector
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Antibodies from Article