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Rapid, specific, no-wash, far-red fluorogen activation in subcellular compartments by targeted fluorogen activating proteins.
Telmer CA, Verma R, Teng H, Andreko S, Law L, Bruchez MP
ACS Chem Biol. 2015 May 15;10(5):1239-46. doi: 10.1021/cb500957k. Epub 2015 Feb 16.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
73205pcDNA3.1-NLS-myc-dL5-2xG4S-mCer3Expresses myc-dL5(E52D)-mCer3 fusion protein in nuclei of mammalian cells. (MBIC5, dL5**, FAP)
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73206pcDNA3.1-kappa-myc-dL5-2xG4S-TMstExpresses myc-dL5(E52D)-TM (PDGFR derived) on the surface of mammalian cells, with an Igk-leader. (MBIC5, dL5**, FAP)
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73207pcDNA3.1-KozATG-dL5-2XG4S-mCer3Expresses dL5(E52D)-mCer3 fusion protein in cytosol of mammalian cells, penetrates nucleus. (MBIC5, dL5**, FAP)
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73208pcDNA3.1-COXIV-COX8-dL5-2XG4S-mCer3Expresses dL5(E52D)-mCer3 fusion protein in mitochondria of mammalian cells. (MBIC5, dL5**, FAP)
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73209pcDNA3.1-kappa-myc-dL5-2XG4S-mCer3-KDELExpresses myc-dL5(E52D)-mCer3 fusion protein in endoplasmic reticulum of mammalian cells with an Igk-leader. (MBIC5, dL5**, FAP)
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73210pcDNA3.1-KozATG-myc-dL5-2XG4S-mCer3-SKLExpresses myc-dL5(E52D)-mCer3 fusion protein in peroxisomes of mammalian cells with a C-terminal PTS (SKL). (MBIC5, dL5**, FAP)
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